FastQCFastQC Report
Thu 26 May 2016
SRR1547808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547808_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3834861
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249020.649358607782655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40220.10487994219347194No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC40190.10480171250014017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA19950.074.185474
GCTAGCG1800.071.111111
CGTTTTT109200.070.3662951
ACGGGAT23100.068.917755
GTCGACG700.068.571431
CGTTAGG7000.068.02
TTACGGG16250.067.692313
GCGCGAC1600.067.59
TTAGGGA61950.065.924134
ATAGGGA57800.065.743944
TAGGGAC45150.064.584725
TAGGGAT67400.064.510385
TAGCGGG26200.064.274813
ACGGGAC14950.064.214055
GTAGCGG9000.064.02
GCGTAAG2450.063.673471
GGCGATA6000.063.3333328
CGGGACT16150.062.9102176
AGGGATT77750.062.302256
TAGTTCG1350.062.222221
Produced by FastQC (version 0.11.5)