##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547808_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3834861
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70948725390568	31.0	31.0	33.0	30.0	34.0
2	32.15352551239797	33.0	31.0	34.0	30.0	34.0
3	32.30651671599049	34.0	31.0	34.0	30.0	34.0
4	35.901609732399685	37.0	35.0	37.0	35.0	37.0
5	35.840256270044726	37.0	35.0	37.0	35.0	37.0
6	35.88880509619514	37.0	35.0	37.0	35.0	37.0
7	36.20660331626101	37.0	35.0	37.0	35.0	37.0
8	36.17447829269431	37.0	35.0	37.0	35.0	37.0
9	38.04148520637384	39.0	39.0	39.0	35.0	39.0
10-11	37.419107107141556	39.0	37.0	39.0	34.5	39.0
12-13	36.764643751103364	39.0	35.0	39.0	33.0	39.0
14-15	37.857222986700165	40.0	36.0	41.0	33.0	41.0
16-17	38.021342103403484	40.0	36.0	41.0	33.0	41.0
18-19	37.91797199429132	40.0	36.0	41.0	33.5	41.0
20-21	37.746531099823436	40.0	35.0	41.0	33.0	41.0
22-23	37.550455674925374	39.5	35.0	41.0	33.0	41.0
24-25	37.46533681403315	39.0	35.0	41.0	33.0	41.0
26-27	37.42728093664934	39.0	35.0	41.0	33.0	41.0
28-29	37.38871434975088	39.0	35.0	41.0	33.0	41.0
30-31	37.20940459119639	39.0	35.0	41.0	32.5	41.0
32-33	36.93163467984889	39.0	35.0	41.0	31.5	41.0
34-35	36.695759768085466	39.0	35.0	41.0	31.0	41.0
36-37	36.509466053658784	39.0	35.0	41.0	30.5	41.0
38-39	36.43245700430863	39.0	35.0	41.0	30.0	41.0
40-41	36.28571178981455	39.0	35.0	41.0	30.0	41.0
42-43	36.227802520091345	39.0	35.0	41.0	30.0	41.0
44-45	36.119226485653584	38.0	35.0	41.0	30.0	41.0
46-47	36.021630119057775	38.0	35.0	41.0	30.0	41.0
48-49	35.912425509034094	38.0	35.0	40.0	29.5	41.0
50-51	35.74129270917512	38.0	35.0	40.0	29.0	41.0
52-53	35.55664286137099	37.5	35.0	40.0	29.0	41.0
54-55	35.34068418646726	37.0	34.5	40.0	28.0	41.0
56-57	35.028721119227	36.5	34.0	40.0	27.5	41.0
58-59	34.6979093896754	36.0	34.0	40.0	26.0	41.0
60-61	34.362754608315655	36.0	34.0	39.0	26.0	41.0
62-63	33.959950178115974	35.0	33.5	39.0	25.5	40.5
64-65	33.6165894930742	35.0	33.0	38.5	25.0	40.0
66-67	33.28338106648455	35.0	33.0	38.0	23.5	40.0
68-69	32.79122594013186	35.0	32.5	37.0	22.0	40.0
70-71	32.48087831605891	35.0	32.0	36.5	21.5	39.0
72-73	32.20434977956177	35.0	32.0	36.0	20.0	39.0
74-75	31.894016367216437	35.0	32.0	36.0	20.0	38.5
76-77	29.853963546527503	33.0	29.0	34.5	17.0	36.0
78-79	30.956324231830045	34.0	30.5	35.0	18.0	37.0
80-81	31.13227937075164	34.5	31.0	35.0	18.0	36.5
82-83	30.945581730341726	34.5	31.0	35.0	17.5	36.0
84-85	30.71161288505633	34.0	31.0	35.0	16.0	36.0
86	30.289466032797538	34.0	30.0	35.0	12.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	14.0
10	22.0
11	42.0
12	98.0
13	194.0
14	623.0
15	1847.0
16	4558.0
17	9381.0
18	16812.0
19	23743.0
20	27759.0
21	28406.0
22	27370.0
23	27739.0
24	30577.0
25	36063.0
26	43716.0
27	52793.0
28	64031.0
29	79854.0
30	97919.0
31	119018.0
32	142917.0
33	179433.0
34	384437.0
35	370130.0
36	342655.0
37	566015.0
38	774472.0
39	380809.0
40	1411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.40811648714256	24.4793748717359	31.405675459944966	13.706833181176581
2	29.62177768633596	26.163269020702444	31.381398178447668	12.833555114513928
3	27.92938257736069	25.312208186946016	32.96450640583844	13.79390282985485
4	26.986793002406085	27.051697571307017	31.670300435921927	14.29120899036497
5	24.52599976896164	31.46922404749481	30.441494489630784	13.563281693912765
6	23.053117179475343	39.88832972042533	26.362415743360714	10.696137356738616
7	89.30662675909244	3.207078431265175	6.00525025548514	1.4810445541572432
8	89.792563537505	2.775015834993759	5.773220985063083	1.6591996424381483
9	85.2508343848708	5.691236266451378	6.869349371463529	2.1885799772142978
10-11	37.2589514978509	30.25848916036331	20.815917447855345	11.666641893930445
12-13	28.08825404623531	25.78398539086554	28.659604611483964	17.468155951415188
14-15	15.381717877127748	32.3402073764864	34.31506383151827	17.96301091486758
16-17	21.829461354661877	29.343332131203713	30.492682785634212	18.334523728500198
18-19	22.7550229330346	30.415118044695756	29.319967529461955	17.509891492807693
20-21	24.827627389884537	30.468392465854695	29.77693324477732	14.927046899483448
22-23	20.812579647606526	25.881850737223594	32.019309174439435	21.286260440730445
24-25	18.737589185109968	29.6789114390326	33.63884636235838	17.944653013499053
26-27	18.098283092920447	35.28018355815243	28.60383987842063	18.017693470506494
28-29	16.640016417804972	30.046408461740853	34.3248164666203	18.988758653833866
30-31	21.528016269690088	30.571016785223765	29.870026579842136	18.030940365244007
32-33	24.00656764352085	29.681401750937	28.723765998298244	17.588264607243914
34-35	19.233956589300107	29.735367201053702	30.619088931776144	20.411587277870044
36-37	21.58075612127793	30.657056409606504	29.409762179124616	18.352425289990954
38-39	18.866655140825184	32.23084226520857	28.59125793607643	20.311244657889816
40-41	20.064182248065837	28.927137124396424	28.84500898468028	22.16367164285746
42-43	20.01712708752677	28.13485286689661	29.10271063279738	22.74530941277924
44-45	19.153080124677267	32.13591053235045	28.33968428060365	20.371325062368623
46-47	20.264802296615184	30.59721069420769	28.82015801876522	20.317828990411908
48-49	19.87872572174063	28.923864515558712	31.411347112711518	19.786062649989137
50-51	18.669164280009106	31.667914430275307	28.965782592902322	20.697138696813262
52-53	20.55984558501599	28.637022306675526	30.67815235024164	20.12497975806685
54-55	20.75663237859208	28.773259317612816	30.447087912704006	20.023020391091098
56-57	21.103281188027413	27.63822469706203	29.696460966903366	21.56203314800719
58-59	22.0883625247434	29.29528606121578	28.85326221732678	19.76308919671404
60-61	22.303976597848006	30.376733341834296	27.459952264241128	19.859337796076574
62-63	20.91178793703344	28.61661739499815	30.776487074759686	19.695107593208725
64-65	19.50155429362368	31.812443788705767	29.708351880289797	18.977650037380755
66-67	18.269984231501482	30.67352376005284	26.731699010733372	24.3247929977123
68-69	17.69330100882405	32.87416414832246	26.83474055513355	22.597794287719946
70-71	18.968823120316486	31.076928212000382	29.530913897531097	20.423334770152035
72-73	20.93915529141734	31.26789993170548	27.921820373671952	19.871124403205226
74-75	19.797679759448908	32.302957004178246	26.757645192355085	21.14171804401776
76-77	22.808584196402425	30.01777639398143	27.19085515746203	19.982784252154122
78-79	18.222916032680196	30.623261182087173	30.18969918336023	20.9641236018724
80-81	17.01284609794201	29.684335364436937	30.904653389001584	22.398165148619466
82-83	18.309255537554034	29.116883245572655	30.257511289196664	22.316349927676647
84-85	17.408336312580822	29.148475003396474	29.155711250029665	24.287477433993043
86	17.97809620739839	28.96136261522908	30.493282546616424	22.56725863075611
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2891.0
1	3642.5
2	11053.5
3	11201.5
4	5261.5
5	7523.5
6	11060.5
7	12907.0
8	14018.5
9	15529.5
10	15260.0
11	13941.0
12	12529.5
13	11421.0
14	11074.0
15	10721.0
16	10224.5
17	9939.5
18	9741.5
19	10478.5
20	11755.0
21	12238.0
22	12950.5
23	15698.5
24	19268.0
25	24223.5
26	31050.5
27	36709.0
28	44518.5
29	50075.0
30	57574.0
31	72257.0
32	81686.5
33	86172.0
34	93863.0
35	112490.0
36	125666.0
37	126554.5
38	133082.5
39	142863.5
40	157170.5
41	175240.0
42	186539.5
43	189935.0
44	193271.0
45	198412.0
46	199849.0
47	203389.5
48	197579.0
49	175708.5
50	163557.0
51	148717.5
52	127679.0
53	111862.0
54	94557.5
55	82813.0
56	73216.0
57	67677.0
58	60863.0
59	45925.5
60	32285.5
61	23492.0
62	18526.5
63	13692.0
64	10546.0
65	8241.5
66	5288.5
67	4115.0
68	2787.0
69	1854.0
70	1387.0
71	1050.0
72	830.5
73	568.5
74	422.0
75	241.0
76	146.0
77	132.0
78	102.0
79	68.0
80	68.5
81	56.0
82	40.5
83	29.5
84	19.5
85	15.0
86	12.0
87	9.0
88	4.5
89	2.5
90	1.0
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3834861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.406586467933774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53150312031343	11.790892203440524
2	10.340580841966403	3.186261057408297
3	3.5127743171767327	1.6235958377976107
4	1.6547115883367123	1.019738286608064
5	0.9889902428738641	0.7618481846389507
6	0.6724811201344131	0.6216383115242278
7	0.5150237076246853	0.5554330099188889
8	0.41915323965377294	0.5166176504032336
9	0.3413367348806958	0.4732950528559465
>10	2.7453406350498955	8.687103523937076
>50	0.6597276974869604	7.4384843326922985
>100	1.5604920345283684	56.752535515097904
>500	0.05360330577491453	4.779235543596734
>1k	0.0041101576313033504	1.1362874189588867
>5k	0.0	0.0
>10k+	1.7125656797097294E-4	0.6570340711213997
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24902	0.649358607782655	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4022	0.10487994219347194	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC	4019	0.10480171250014017	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07165839909190971	0.0
2	0.0	0.0	0.0	0.2334895580309169	0.0
3	0.0	0.0	0.0	0.335109929668898	0.0
4	0.0	0.0	0.0	0.48705807068365714	0.0
5	0.0	0.0	0.0	0.838518006258897	0.0
6	0.0	0.0	0.0	1.0927645095871794	0.0
7	0.0	0.0	0.0	1.3013770251385903	0.0
8	0.0	0.0	0.0	1.71453411218816	0.0
9	0.0	0.0	0.0	1.858059522887531	0.0
10	0.0	0.0	0.0	2.117051960944608	0.0
11	0.0	0.0	0.0	2.601945676779419	0.0
12	0.0	0.0	0.0	2.921566127168625	0.0
13	0.0	0.0	0.0	3.0357814794330227	0.0
14	0.0	0.0	0.0	3.081676232854333	0.0
15	0.0	0.0	0.0	3.1533085553817988	0.0
16	0.0	0.0	0.0	3.3046308588499036	0.0
17	0.0	0.0	0.0	3.507011075499216	0.0
18	0.0	0.0	0.0	3.7258456043126467	0.0
19	0.0	0.0	0.0	3.893257148042654	0.0
20	0.0	0.0	0.0	4.064997401470353	0.0
21	0.0	0.0	0.0	4.274757285857297	0.0
22	0.0	0.0	0.0	4.515365745981406	0.0
23	0.0	0.0	0.0	4.749298605607869	0.0
24	0.0	0.0	0.0	4.923933357688845	0.0
25	0.0	0.0	0.0	5.084773607178983	0.0
26	0.0	0.0	0.0	5.239225098380358	0.0
27	0.0	0.0	0.0	5.395449795963922	0.0
28	0.0	0.0	0.0	5.567294355649397	0.0
29	0.0	0.0	0.0	5.7710044770853495	0.0
30	0.0	0.0	0.0	6.007466763462874	0.0
31	0.0	0.0	0.0	6.213941000729883	0.0
32	0.0	0.0	0.0	6.400936044357279	0.0
33	0.0	0.0	0.0	6.585193048718063	0.0
34	0.0	0.0	0.0	6.78321847910524	0.0
35	0.0	0.0	0.0	7.008076694305218	0.0
36	0.0	0.0	0.0	7.213951170590017	0.0
37	0.0	0.0	0.0	7.427570386514661	0.0
38	0.0	0.0	0.0	7.667735545043223	0.0
39	2.607656444392639E-5	0.0	0.0	8.009990453369758	0.0
40	2.607656444392639E-5	0.0	0.0	8.249842693124991	0.0
41	2.607656444392639E-5	0.0	0.0	8.48646143888918	0.0
42	2.607656444392639E-5	0.0	0.0	8.743654593999626	0.0
43	2.607656444392639E-5	0.0	0.0	8.976674773870553	0.0
44	2.607656444392639E-5	0.0	0.0	9.211442083559222	0.0
45	2.607656444392639E-5	0.0	0.0	9.443836425883493	0.0
46	2.607656444392639E-5	0.0	0.0	9.690416419265262	0.0
47	2.607656444392639E-5	0.0	0.0	9.946201439895736	0.0
48	2.607656444392639E-5	0.0	0.0	10.196979760152976	0.0
49	2.607656444392639E-5	0.0	0.0	10.457849710850015	0.0
50	2.607656444392639E-5	0.0	0.0	10.731705790640131	0.0
51	2.607656444392639E-5	0.0	0.0	11.000085791897021	0.0
52	2.607656444392639E-5	0.0	0.0	11.309953607184198	0.0
53	5.215312888785278E-5	0.0	0.0	11.587408252867574	0.0
54	5.215312888785278E-5	0.0	0.0	11.872816250706348	0.0
55	5.215312888785278E-5	0.0	0.0	12.162213962905044	0.0
56	5.215312888785278E-5	0.0	0.0	12.447491577921598	0.0
57	5.215312888785278E-5	0.0	0.0	12.730969909991522	0.0
58	5.215312888785278E-5	0.0	0.0	13.008502785368231	0.0
59	5.215312888785278E-5	0.0	0.0	13.308435429602273	0.0
60	5.215312888785278E-5	0.0	0.0	13.636947988466858	0.0
61	5.215312888785278E-5	0.0	0.0	13.946242119336269	0.0
62	7.822969333177917E-5	0.0	0.0	14.298719040924821	0.0
63	7.822969333177917E-5	0.0	0.0	14.625693082487214	0.0
64	7.822969333177917E-5	0.0	0.0	14.971833398915892	0.0
65	7.822969333177917E-5	0.0	0.0	15.282014132976398	0.0
66	7.822969333177917E-5	0.0	0.0	15.585884338441472	0.0
67	7.822969333177917E-5	0.0	0.0	15.90399234809293	0.0
68	7.822969333177917E-5	0.0	0.0	16.276469994609975	0.0
69	1.0430625777570557E-4	0.0	0.0	16.784989077830982	0.0
70	1.0430625777570557E-4	0.0	0.0	17.130451403584118	0.0
71	1.0430625777570557E-4	0.0	0.0	17.463814203435273	0.0
72	1.3038282221963194E-4	0.0	0.0	17.798193989299744	0.0
73	1.3038282221963194E-4	0.0	0.0	18.126211093439892	0.0
74	1.3038282221963194E-4	0.0	0.0	18.513135156658873	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	1995	0.0	74.18547	4
GCTAGCG	180	0.0	71.11111	1
CGTTTTT	10920	0.0	70.366295	1
ACGGGAT	2310	0.0	68.91775	5
GTCGACG	70	0.0	68.57143	1
CGTTAGG	700	0.0	68.0	2
TTACGGG	1625	0.0	67.69231	3
GCGCGAC	160	0.0	67.5	9
TTAGGGA	6195	0.0	65.92413	4
ATAGGGA	5780	0.0	65.74394	4
TAGGGAC	4515	0.0	64.58472	5
TAGGGAT	6740	0.0	64.51038	5
TAGCGGG	2620	0.0	64.27481	3
ACGGGAC	1495	0.0	64.21405	5
GTAGCGG	900	0.0	64.0	2
GCGTAAG	245	0.0	63.67347	1
GGCGATA	600	0.0	63.333332	8
CGGGACT	1615	0.0	62.910217	6
AGGGATT	7775	0.0	62.30225	6
TAGTTCG	135	0.0	62.22222	1
>>END_MODULE