FastQCFastQC Report
Thu 26 May 2016
SRR1547807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547807_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2658371
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155030.5831766897848344No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34590.13011727858903066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG150.001309981280.02
CGTAAGG4050.078.024692
TCGCGCG700.074.285711
TACGGGA14800.072.972974
CTATGCG900.071.111111
CGTTTTT74900.069.639521
ATACGCG750.069.3333361
AGTTGCG1850.069.1891861
GTACGGG12150.068.806593
GCGTAAG2500.068.81
ATAGGGA43000.067.255814
TCGCGAT600.066.6666641
CGGGAGT6750.066.370386
AGGGATT58950.065.750636
CTATACG1100.065.4545441
TAAGGGA46450.065.016144
ACGGGAC11700.064.957265
CGGGATA7100.064.225356
GGGCGAT18900.064.126987
TAGGGAC30950.064.103395
Produced by FastQC (version 0.11.5)