##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547807_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2658371
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7570316558524	31.0	31.0	33.0	30.0	34.0
2	32.20465239802872	33.0	31.0	34.0	30.0	34.0
3	32.35778076122558	34.0	31.0	34.0	30.0	34.0
4	35.94068660845307	37.0	35.0	37.0	35.0	37.0
5	35.88770228083288	37.0	35.0	37.0	35.0	37.0
6	35.94449081787305	37.0	35.0	37.0	35.0	37.0
7	36.24112661475768	37.0	35.0	37.0	35.0	37.0
8	36.20741235892206	37.0	36.0	37.0	35.0	37.0
9	38.09497696145497	39.0	39.0	39.0	37.0	39.0
10-11	37.497282922511566	39.0	37.5	39.0	34.5	39.0
12-13	36.86169838596645	39.0	35.0	39.0	33.0	39.0
14-15	37.95864629128139	40.0	36.0	41.0	33.0	41.0
16-17	38.09786199894597	40.0	36.0	41.0	33.5	41.0
18-19	37.990329416022064	40.0	36.0	41.0	33.5	41.0
20-21	37.82842406120139	40.0	35.0	41.0	33.5	41.0
22-23	37.63273880884195	39.5	35.0	41.0	33.0	41.0
24-25	37.564490810349646	39.0	35.0	41.0	33.0	41.0
26-27	37.526158312741146	39.5	35.0	41.0	33.0	41.0
28-29	37.50718278223769	40.0	35.0	41.0	33.0	41.0
30-31	37.34923078832864	39.5	35.0	41.0	33.0	41.0
32-33	37.06780167252803	39.0	35.0	41.0	32.0	41.0
34-35	36.80432565657691	39.0	35.0	41.0	31.0	41.0
36-37	36.64163090855264	39.0	35.0	41.0	31.0	41.0
38-39	36.566695167830225	39.0	35.0	41.0	31.0	41.0
40-41	36.412521615681186	39.0	35.0	41.0	30.0	41.0
42-43	36.37767696833888	39.0	35.0	41.0	30.0	41.0
44-45	36.267280037285985	39.0	35.0	41.0	30.0	41.0
46-47	36.18242092619879	38.0	35.0	41.0	30.0	41.0
48-49	36.080154162078955	38.0	35.0	40.5	30.0	41.0
50-51	35.90517971344105	38.0	35.0	40.0	29.5	41.0
52-53	35.713187136031806	38.0	35.0	40.0	29.0	41.0
54-55	35.52448153399206	37.5	35.0	40.0	29.0	41.0
56-57	35.23323531591339	37.0	34.5	40.0	28.0	41.0
58-59	34.91476584720492	36.5	34.0	40.0	27.0	41.0
60-61	34.59752194106842	36.0	34.0	39.5	26.5	41.0
62-63	34.22095091317202	35.5	33.5	39.0	26.0	41.0
64-65	33.90393459001773	35.0	33.0	39.0	26.0	40.0
66-67	33.58924770094167	35.0	33.0	38.0	25.0	40.0
68-69	33.13424743950337	35.0	33.0	37.5	24.0	40.0
70-71	32.860952064252885	35.0	33.0	37.0	24.0	39.0
72-73	32.58355022681184	35.0	33.0	36.5	23.0	39.0
74-75	32.28972291677873	35.0	32.5	36.0	22.5	38.5
76-77	30.224890543870664	33.0	29.5	34.5	19.0	36.0
78-79	31.33552314556546	34.0	31.0	35.0	20.0	37.0
80-81	31.510771069952234	35.0	32.0	35.0	20.0	37.0
82-83	31.328999789720847	35.0	32.0	35.0	19.0	36.0
84-85	31.107905743780684	34.5	32.0	35.0	18.5	36.0
86	30.67915087848912	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	15.0
11	25.0
12	36.0
13	101.0
14	324.0
15	1067.0
16	2645.0
17	5826.0
18	11179.0
19	16399.0
20	19255.0
21	19759.0
22	17977.0
23	17467.0
24	18941.0
25	22167.0
26	26612.0
27	32225.0
28	40257.0
29	49504.0
30	62334.0
31	75402.0
32	93078.0
33	119545.0
34	266115.0
35	259941.0
36	234405.0
37	399455.0
38	562719.0
39	282501.0
40	1081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.575416298176588	24.67285416520117	31.389937672356496	13.361791864265749
2	29.3225813853672	25.887771119982876	32.10499964075744	12.684647853892479
3	28.9801160184188	25.212846513898924	32.225938366014375	13.5810991016679
4	26.527749512765524	27.678454211244407	31.881027892645537	13.912768383344536
5	23.795775683679967	32.218941599949744	30.014659353416057	13.97062336295423
6	21.991437613485854	40.694846580857224	26.933637178557845	10.380078627099076
7	89.35874639017653	3.371952221868204	5.759429364825301	1.5098720231299543
8	90.11360716769781	2.602082252627643	5.730351406933043	1.5539591727415023
9	86.13429051099338	5.2668344636621445	6.484422226995404	2.114452798349064
10-11	36.82627443648761	31.183025243654853	19.88956770894657	12.101132610910968
12-13	28.23966256026717	26.074539633482306	28.59252903375789	17.093268772492628
14-15	15.826045348824525	32.25210100471303	34.63634308379079	17.285510562671654
16-17	21.75127173746629	28.236408687876903	31.603433079882382	18.408886494774432
18-19	22.814178306940605	29.813916116298287	29.86720062775286	17.504704949008246
20-21	25.538910859319486	28.79560452623054	29.886573394007083	15.778911220442895
22-23	21.174696835016633	25.613618264719257	32.52435420037308	20.687330699891024
24-25	19.629182683681094	28.61822145968339	33.87975192326429	17.872843933371225
26-27	18.139586235329833	34.92796904570506	29.15114180827281	17.7813029106923
28-29	16.276866547220084	30.25636752733159	34.784083937117884	18.682681988330447
30-31	21.14501700477473	29.984340033802653	31.06552847589746	17.80511448552516
32-33	24.11087466723042	29.477657557955606	29.019801976473563	17.391665798340412
34-35	18.85049152281604	29.756117562221373	31.080123880376366	20.313267034586218
36-37	21.586133011532247	30.38906157191754	29.999349225521943	18.025456191028265
38-39	19.698924642196292	31.68993342163302	28.78328495157373	19.82785698459696
40-41	19.646975535017496	28.60227184241778	29.98537450190361	21.76537812066111
42-43	19.870533495889024	28.22175685786521	29.50551296263765	22.402196683608118
44-45	19.411549403751398	31.82385001942919	28.627287161949933	20.13731341486948
46-47	20.057885073227176	31.157803030502514	28.982184954620706	19.802126941649604
48-49	20.86057589403435	28.88590418718832	30.717044385452596	19.536475533324733
50-51	18.900390502303853	31.053886007633995	29.570834921085133	20.47488856897702
52-53	20.79517870154316	28.467132691411397	30.370553997165935	20.367134609879507
54-55	20.70583827464263	28.057502131944712	31.137414604658264	20.099244988754393
56-57	21.346079986578246	27.793505872581363	29.803082414004667	21.05733172683572
58-59	21.790731993389937	29.291547342338596	29.081249381670204	19.836471282601263
60-61	22.40798970497346	30.515962595138152	27.80787557492916	19.268172124959232
62-63	21.418925349396304	28.679066992530387	30.105786588854606	19.796221069218706
64-65	19.42608838269752	30.466552636934424	30.508363956723873	19.59899502364418
66-67	18.68382930749696	30.933210601530035	26.834497517464644	23.548462573508363
68-69	17.90519457216468	33.10882867741184	27.077823223319847	21.90815352710363
70-71	18.62458249807871	31.262773329982913	29.259140278012364	20.853503893926018
72-73	21.472924584266078	30.884853919938188	27.74614604206862	19.896075453727114
74-75	19.96643809310288	32.83097054549572	26.002954440896325	21.19963692050508
76-77	22.781733625592516	30.315162932487606	26.957862540631083	19.9452409012888
78-79	18.60641347652378	30.706379959757307	29.37024215205477	21.316964411664134
80-81	16.999226217860485	29.958120969571215	30.222098420423638	22.82055439214466
82-83	18.745107436095264	29.519675771365243	30.423424721380123	21.31179207115937
84-85	17.182740858969648	28.53333864987242	29.96062250152443	24.323297989633502
86	17.811208443065322	29.280676023023123	30.012477566148593	22.895637967762962
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1903.0
1	2303.0
2	6619.0
3	6742.5
4	3380.0
5	4992.0
6	7174.5
7	8175.0
8	8728.5
9	9493.0
10	9434.5
11	8513.5
12	7537.5
13	7127.5
14	7042.0
15	6907.0
16	6888.0
17	7059.0
18	7260.5
19	7884.5
20	9274.0
21	10186.0
22	10593.0
23	10954.0
24	12635.0
25	16109.0
26	20173.0
27	25564.5
28	34212.5
29	39786.0
30	44523.5
31	51877.0
32	58455.5
33	66369.0
34	70831.0
35	75762.0
36	80182.0
37	83436.5
38	89596.0
39	98070.0
40	110204.5
41	122026.0
42	132658.0
43	138034.0
44	141336.5
45	140647.5
46	138889.0
47	141816.0
48	135592.0
49	121745.5
50	114817.0
51	98642.5
52	78778.5
53	70130.0
54	63442.0
55	58262.0
56	52005.0
57	49199.0
58	43641.0
59	31288.0
60	21631.0
61	16921.0
62	13700.0
63	10515.0
64	8703.0
65	6546.5
66	3527.0
67	2306.0
68	1707.0
69	1153.5
70	627.0
71	413.0
72	342.5
73	255.5
74	186.0
75	130.0
76	97.0
77	50.5
78	39.0
79	44.0
80	31.0
81	15.0
82	10.0
83	10.0
84	11.0
85	6.5
86	3.0
87	4.0
88	4.5
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2658371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.908950417712644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39722846728776	12.472176205721713
2	9.278852811097734	2.9523361861001485
3	3.068899842350629	1.4646892628664692
4	1.449396558602462	0.9223351194563963
5	0.8598434807143103	0.683960365083871
6	0.6207482948945688	0.5925272307214419
7	0.45790851661175735	0.5099390720637357
8	0.3473169532412964	0.44203585506774445
9	0.2916107904210328	0.4175299445470384
>10	2.588349640846709	8.904530836184927
>50	0.879009185384114	10.249587958685094
>100	1.7453477312584855	57.667561376249395
>500	0.011437090921227185	1.1539554594450077
>1k	0.003812363640409062	0.9791677114816368
>5k	0.0	0.0
>10k+	2.382727275255664E-4	0.5876674163253385
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15503	0.5831766897848344	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3459	0.13011727858903066	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.76170218528565E-5	0.0	0.0	0.05473276679590622	0.0
2	3.76170218528565E-5	0.0	0.0	0.18488766240678972	0.0
3	3.76170218528565E-5	0.0	0.0	0.2657642593904312	0.0
4	3.76170218528565E-5	0.0	0.0	0.3918565166412062	0.0
5	3.76170218528565E-5	0.0	0.0	0.6758650316302728	0.0
6	3.76170218528565E-5	0.0	0.0	0.8867460561373864	0.0
7	3.76170218528565E-5	0.0	0.0	1.0363113350243438	0.0
8	3.76170218528565E-5	0.0	0.0	1.3369465736723731	0.0
9	3.76170218528565E-5	0.0	0.0	1.4379482773472927	0.0
10	3.76170218528565E-5	0.0	0.0	1.6139207055749556	0.0
11	3.76170218528565E-5	0.0	0.0	1.9663922003362209	0.0
12	3.76170218528565E-5	0.0	0.0	2.207743012544148	0.0
13	3.76170218528565E-5	0.0	0.0	2.2920803755382524	0.0
14	3.76170218528565E-5	0.0	0.0	2.3277789292766133	0.0
15	3.76170218528565E-5	0.0	0.0	2.384806334405544	0.0
16	3.76170218528565E-5	0.0	0.0	2.507701144798826	0.0
17	3.76170218528565E-5	0.0	0.0	2.682018424064963	0.0
18	3.76170218528565E-5	0.0	0.0	2.8659656609254314	0.0
19	3.76170218528565E-5	0.0	0.0	3.000032726809012	0.0
20	3.76170218528565E-5	0.0	0.0	3.1385762182930823	0.0
21	3.76170218528565E-5	0.0	0.0	3.3139091571492467	0.0
22	3.76170218528565E-5	0.0	0.0	3.515197841083882	0.0
23	3.76170218528565E-5	0.0	0.0	3.7187435463296885	0.0
24	3.76170218528565E-5	0.0	0.0	3.864321420900243	0.0
25	3.76170218528565E-5	0.0	0.0	3.9929340186151596	0.0
26	3.76170218528565E-5	0.0	0.0	4.11883819075667	0.0
27	3.76170218528565E-5	0.0	0.0	4.259864405683029	0.0
28	3.76170218528565E-5	0.0	0.0	4.401229173806064	0.0
29	3.76170218528565E-5	0.0	0.0	4.5760354743562885	0.0
30	3.76170218528565E-5	0.0	0.0	4.772433945450052	0.0
31	3.76170218528565E-5	0.0	0.0	4.946939309825453	0.0
32	3.76170218528565E-5	0.0	0.0	5.108504418683472	0.0
33	7.5234043705713E-5	0.0	0.0	5.268451995601818	0.0
34	7.5234043705713E-5	0.0	0.0	5.441377445059399	0.0
35	7.5234043705713E-5	0.0	0.0	5.64353132049665	0.0
36	7.5234043705713E-5	0.0	0.0	5.829359408449761	0.0
37	7.5234043705713E-5	0.0	0.0	6.0189115815663055	0.0
38	7.5234043705713E-5	0.0	0.0	6.221178308069114	0.0
39	7.5234043705713E-5	0.0	0.0	6.496121120791643	0.0
40	7.5234043705713E-5	0.0	0.0	6.7217856348869285	0.0
41	7.5234043705713E-5	0.0	0.0	6.943048957425431	0.0
42	7.5234043705713E-5	0.0	0.0	7.175183599279408	0.0
43	7.5234043705713E-5	0.0	0.0	7.382867176929029	0.0
44	7.5234043705713E-5	0.0	0.0	7.5956290525287855	0.0
45	7.5234043705713E-5	0.0	0.0	7.801958417391703	0.0
46	7.5234043705713E-5	0.0	0.0	8.025629229328787	0.0
47	7.5234043705713E-5	0.0	0.0	8.293989063227066	0.0
48	7.5234043705713E-5	0.0	0.0	8.537596896746166	0.0
49	7.5234043705713E-5	0.0	0.0	8.781881836658615	0.0
50	7.5234043705713E-5	0.0	0.0	9.038505159738802	0.0
51	7.5234043705713E-5	0.0	0.0	9.299642525441332	0.0
52	7.5234043705713E-5	0.0	0.0	9.608666359962548	0.0
53	7.5234043705713E-5	0.0	0.0	9.873941598068892	0.0
54	7.5234043705713E-5	0.0	0.0	10.145799814999487	0.0
55	7.5234043705713E-5	0.0	0.0	10.419049861738635	0.0
56	7.5234043705713E-5	0.0	0.0	10.684626036019804	0.0
57	7.5234043705713E-5	0.0	0.0	10.967882210571812	0.0
58	7.5234043705713E-5	0.0	0.0	11.230599491192162	0.0
59	7.5234043705713E-5	0.0	0.0	11.512087665717088	0.0
60	7.5234043705713E-5	0.0	0.0	11.817725968271546	0.0
61	7.5234043705713E-5	0.0	0.0	12.127163590033144	0.0
62	1.50468087411426E-4	0.0	0.0	12.479710318838116	0.0
63	1.8808510926428252E-4	0.0	0.0	12.827404451824068	0.0
64	1.8808510926428252E-4	0.0	0.0	13.161293137790022	0.0
65	1.8808510926428252E-4	0.0	0.0	13.485965653402028	0.0
66	1.8808510926428252E-4	0.0	0.0	13.78663850907191	0.0
67	1.8808510926428252E-4	0.0	0.0	14.103712386269637	0.0
68	3.00936174822852E-4	0.0	0.0	14.442265582945344	0.0
69	3.00936174822852E-4	0.0	0.0	14.865381844746276	0.0
70	3.00936174822852E-4	0.0	0.0	15.214806360737459	0.0
71	3.00936174822852E-4	0.0	0.0	15.549108833943794	0.0
72	3.3855319667570855E-4	0.0	0.0	15.880401945401902	0.0
73	3.3855319667570855E-4	0.0	0.0	16.211544588772597	0.0
74	3.3855319667570855E-4	0.0	0.0	16.590084679677894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	15	0.0013099812	80.0	2
CGTAAGG	405	0.0	78.02469	2
TCGCGCG	70	0.0	74.28571	1
TACGGGA	1480	0.0	72.97297	4
CTATGCG	90	0.0	71.11111	1
CGTTTTT	7490	0.0	69.63952	1
ATACGCG	75	0.0	69.333336	1
AGTTGCG	185	0.0	69.189186	1
GTACGGG	1215	0.0	68.80659	3
GCGTAAG	250	0.0	68.8	1
ATAGGGA	4300	0.0	67.25581	4
TCGCGAT	60	0.0	66.666664	1
CGGGAGT	675	0.0	66.37038	6
AGGGATT	5895	0.0	65.75063	6
CTATACG	110	0.0	65.454544	1
TAAGGGA	4645	0.0	65.01614	4
ACGGGAC	1170	0.0	64.95726	5
CGGGATA	710	0.0	64.22535	6
GGGCGAT	1890	0.0	64.12698	7
TAGGGAC	3095	0.0	64.10339	5
>>END_MODULE