FastQCFastQC Report
Thu 26 May 2016
SRR1547806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547806_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3593409
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208280.5796167371985766No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC38720.10775283303403538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG1850.073.513511
CGTTAGG6550.073.282442
TACGGGA14100.072.340424
CGTTTTT99300.071.581061
TATAGCG4100.068.292691
CTACGGG6750.067.555563
GCGGGAT29850.067.537695
CGAACGG5050.067.326732
GCGAATG3100.067.096781
CGGGATT22350.066.756166
CGTAAGG6250.066.562
CGAGGGA25450.066.326134
TAGCGGG27850.065.6373443
TCGAACG3850.065.4545441
AGCGGGA35100.065.071234
TAGGGAG47400.064.810135
TAGGGAC43950.063.799775
TGCGGGC4100.063.4146354
ATCGCGG2650.063.3962252
GTACGGG13750.062.8363653
Produced by FastQC (version 0.11.5)