##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547806_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3593409
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745116684463138	31.0	31.0	33.0	30.0	34.0
2	32.181557123055015	33.0	31.0	34.0	30.0	34.0
3	32.33602325813733	34.0	31.0	34.0	30.0	34.0
4	35.92244773695396	37.0	35.0	37.0	35.0	37.0
5	35.85882792635071	37.0	35.0	37.0	35.0	37.0
6	35.91985855214366	37.0	35.0	37.0	35.0	37.0
7	36.23119578094227	37.0	35.0	37.0	35.0	37.0
8	36.20117609768329	37.0	36.0	37.0	35.0	37.0
9	38.07909258311536	39.0	39.0	39.0	37.0	39.0
10-11	37.47008147416562	39.0	37.0	39.0	34.5	39.0
12-13	36.811036678541186	39.0	35.0	39.0	33.0	39.0
14-15	37.860468568982824	40.0	36.0	41.0	33.0	41.0
16-17	38.04997413319775	40.0	36.0	41.0	33.0	41.0
18-19	37.96681897329249	40.0	36.0	41.0	33.5	41.0
20-21	37.78527993891038	40.0	35.0	41.0	33.0	41.0
22-23	37.62940525278364	39.5	35.0	41.0	33.0	41.0
24-25	37.56371289769687	39.0	35.0	41.0	33.0	41.0
26-27	37.5234797931435	39.5	35.0	41.0	33.0	41.0
28-29	37.49050873418528	39.0	35.0	41.0	33.0	41.0
30-31	37.31190256939858	39.0	35.0	41.0	32.5	41.0
32-33	37.05256735317354	39.0	35.0	41.0	32.0	41.0
34-35	36.80633905018883	39.0	35.0	41.0	31.0	41.0
36-37	36.64377322481243	39.0	35.0	41.0	31.0	41.0
38-39	36.54433923330186	39.0	35.0	41.0	31.0	41.0
40-41	36.391841563262076	39.0	35.0	41.0	30.0	41.0
42-43	36.360562351794634	39.0	35.0	41.0	30.0	41.0
44-45	36.27164956730503	39.0	35.0	41.0	30.0	41.0
46-47	36.21418101863718	38.0	35.0	41.0	30.0	41.0
48-49	36.10627415359621	38.0	35.0	40.5	30.0	41.0
50-51	35.93978795622763	38.0	35.0	40.0	29.5	41.0
52-53	35.75631176412148	38.0	35.0	40.0	29.0	41.0
54-55	35.552862476829105	37.5	35.0	40.0	28.5	41.0
56-57	35.25582587453863	37.0	34.0	40.0	28.0	41.0
58-59	34.944314577049255	36.5	34.0	40.0	27.0	41.0
60-61	34.62935432621224	36.0	34.0	39.5	26.5	41.0
62-63	34.2350724618322	35.5	33.5	39.0	26.0	41.0
64-65	33.84133896252834	35.0	33.0	39.0	25.0	40.0
66-67	33.49138812197554	35.0	33.0	38.5	24.5	40.0
68-69	32.96636675646997	35.0	33.0	37.5	22.5	40.0
70-71	32.622756552343475	35.0	32.5	37.0	21.0	39.0
72-73	32.27683892927301	35.0	32.0	36.5	20.0	39.0
74-75	31.90373138710344	35.0	32.0	36.0	19.5	38.5
76-77	29.85805039170325	33.0	29.0	34.5	16.0	36.0
78-79	30.932286861862927	34.0	30.5	35.0	17.0	37.0
80-81	31.03603875873857	35.0	31.0	35.0	16.0	37.0
82-83	30.812486972676922	34.5	31.0	35.0	13.5	36.0
84-85	30.53609079845907	34.0	31.0	35.0	11.5	36.0
86	30.07854741834286	34.0	30.0	35.0	10.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	17.0
10	18.0
11	19.0
12	44.0
13	176.0
14	427.0
15	1365.0
16	3400.0
17	7590.0
18	14396.0
19	21026.0
20	24738.0
21	25981.0
22	24592.0
23	24259.0
24	26813.0
25	31571.0
26	38140.0
27	47561.0
28	59139.0
29	74715.0
30	94128.0
31	113194.0
32	136793.0
33	172713.0
34	358594.0
35	340797.0
36	310095.0
37	507042.0
38	741591.0
39	391222.0
40	1249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.01213916924013	24.106746546246196	31.720435942582657	13.160678341931018
2	29.527337411355063	25.78799129183458	31.445070683576514	13.23960061323384
3	28.50908427067445	24.58487191410719	33.1596820734851	13.746361741733267
4	25.99826515712517	27.06229655460873	32.60447113033891	14.334967157927196
5	24.108666728446444	31.16786872855275	30.578038848347074	14.14542569465374
6	22.64949522862552	40.34709102136718	26.359871642777094	10.64354210723021
7	89.30789676321287	3.253261735583119	6.012619214790189	1.4262222864138203
8	90.05050635761195	2.499464992713048	5.7167720123147685	1.7332566373602338
9	85.48676201345296	5.337355141037382	6.787204017132479	2.3886788283771763
10-11	36.65846275778794	31.238706754505262	20.33781848935092	11.76501199835588
12-13	27.893359759493002	25.298163944043107	29.378509376472312	17.429966919991575
14-15	16.012468939661474	31.662580018027448	35.15665764737607	17.168293394935006
16-17	21.816497927177227	28.249303099090582	31.40505297337431	18.529146000357876
18-19	22.891132069853445	29.32403464231319	30.19290317356026	17.591930114273104
20-21	24.15227434450128	30.07155600712304	30.082784898685343	15.693384749690336
22-23	20.288742528334513	25.317866683141272	33.18969535613675	21.203695432387462
24-25	18.505046322308427	29.510960205197907	33.73701685502541	18.246976617468256
26-27	17.788414845067734	34.48619402912388	29.525709430793988	18.199681695014398
28-29	16.198684869993926	29.766553153287035	34.76933741747739	19.265424559241655
30-31	21.155162131558082	29.029704105488683	31.128254534899867	18.68687922805336
32-33	23.527686383598414	29.026044627817203	29.23321558998711	18.213053398597264
34-35	18.320541858719672	29.395637958273053	31.01357234870843	21.27024783429885
36-37	21.41747571734807	29.795870717750194	29.96058895605816	18.82606460884358
38-39	19.085901437882523	30.60589540461439	29.374585525889202	20.93361763161388
40-41	19.530743647605934	27.67591999686092	29.901063307850567	22.892273047682576
42-43	19.863255198615022	27.292537531909115	30.16582582166405	22.678381447811812
44-45	18.914476476237464	30.479650382130174	29.045900424916844	21.55997271671552
46-47	19.493620125067867	29.61897741114357	30.029270255626344	20.85813220816222
48-49	19.819271894738392	27.777160907650643	31.43304867327933	20.97051852433163
50-51	18.24417983035051	30.46600039127191	29.964693693370283	21.3251260850073
52-53	20.566459314817767	27.438415721672648	30.643686816613418	21.351438146896164
54-55	20.460000517614333	27.514708178222964	31.16344396087392	20.861847343288783
56-57	20.8312914004501	26.893070062439318	30.665768911916235	21.60986962519435
58-59	21.6643304449897	28.455736599980685	29.546247031718348	20.33368592331126
60-61	21.762134507928266	29.25293224344905	28.740911485444602	20.244021763178086
62-63	20.421610787973204	27.921327630670483	31.123579308673182	20.533482272683127
64-65	19.155779372734916	30.61667903653606	30.475392586816586	19.752149003912443
66-67	18.187590112898363	30.380385310995774	28.000876048342953	23.431148527762915
68-69	17.466686925980316	32.173960715298485	27.264082657999687	23.095269700721516
70-71	18.22045584012285	30.866483609296907	29.883545123864273	21.029515426715967
72-73	20.453307708641013	30.55541409285723	28.700323286327826	20.290954912173927
74-75	19.803729550407425	32.09990847131512	26.780642003178595	21.315719975098855
76-77	21.66175628769227	29.888596037912745	28.16338190281151	20.286265771583476
78-79	17.917929743037877	30.2362325023397	30.02064335008901	21.82519440453341
80-81	16.116158778474702	29.53557471470684	31.09209110346192	23.256175403356536
82-83	17.551341915156332	29.286730233046114	30.94771566498553	22.21421218681202
84-85	16.85933886178835	28.735707513394665	29.898544808008214	24.506408816808776
86	17.167959450204528	29.196175553631665	30.383989131212175	23.25187586495164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1958.0
1	2785.0
2	8813.0
3	8913.5
4	4433.0
5	6459.5
6	9252.0
7	10638.0
8	11880.0
9	12921.0
10	12411.5
11	11647.0
12	10833.0
13	10067.0
14	9659.0
15	9474.0
16	9263.5
17	9216.0
18	9761.5
19	10372.5
20	11116.0
21	11816.0
22	12441.5
23	13811.5
24	16360.0
25	22348.5
26	30440.5
27	34716.5
28	42175.5
29	49266.0
30	55937.0
31	66113.5
32	74391.5
33	82199.0
34	89768.5
35	101974.5
36	109646.0
37	111425.0
38	118249.5
39	131340.0
40	149483.0
41	168972.0
42	184544.5
43	190726.0
44	194038.5
45	195702.0
46	196092.0
47	196017.0
48	190497.5
49	173455.5
50	159819.0
51	143617.5
52	116032.5
53	98429.5
54	86203.0
55	75391.0
56	63485.5
57	56385.0
58	50155.0
59	38273.5
60	28918.5
61	21597.0
62	16154.0
63	11481.0
64	8633.0
65	7100.0
66	4817.0
67	3244.5
68	1990.0
69	1232.5
70	861.5
71	816.0
72	619.5
73	279.5
74	124.5
75	82.0
76	54.0
77	44.5
78	28.5
79	25.0
80	33.0
81	26.5
82	10.0
83	8.0
84	7.5
85	5.5
86	4.0
87	2.5
88	0.5
89	1.5
90	2.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3593409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.68933971251048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9441830449743	12.507689303141442
2	10.88772530482042	3.634178926172895
3	3.9749486775356826	1.9901780645756186
4	1.9409229359414588	1.2957088893492092
5	1.2059537398314408	1.00632858208097
6	0.8145417756258079	0.815649864207036
7	0.5794619985458238	0.6769586700955056
8	0.4396782130593909	0.5870351249550191
9	0.3570840574731842	0.5363547426982431
>10	2.6263932059681614	9.053577326295517
>50	0.6843191853963491	8.330335459775718
>100	1.524754155277117	56.006224387305146
>500	0.015656593413714982	1.6471339829641873
>1k	0.00420876167035349	1.3274510807711175
>5k	0.0	0.0
>10k+	1.683504668141396E-4	0.5851955956123447
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20828	0.5796167371985766	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	3872	0.10775283303403538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7828727539781863E-5	0.0	0.0	0.11501613092191844	0.0
2	2.7828727539781863E-5	0.0	0.0	0.33717286287199705	0.0
3	2.7828727539781863E-5	0.0	0.0	0.49293025091215614	0.0
4	2.7828727539781863E-5	0.0	0.0	0.7069888231481583	0.0
5	2.7828727539781863E-5	0.0	0.0	1.171255484694339	0.0
6	2.7828727539781863E-5	0.0	0.0	1.5143280378047699	0.0
7	2.7828727539781863E-5	0.0	0.0	1.7613914809029532	0.0
8	2.7828727539781863E-5	0.0	0.0	2.228441015203112	0.0
9	2.7828727539781863E-5	0.0	0.0	2.38436537560851	0.0
10	2.7828727539781863E-5	0.0	0.0	2.6888116548937235	0.0
11	2.7828727539781863E-5	0.0	0.0	3.324698079177739	0.0
12	2.7828727539781863E-5	0.0	0.0	3.822498357409357	0.0
13	2.7828727539781863E-5	0.0	0.0	3.995843501254658	0.0
14	2.7828727539781863E-5	0.0	0.0	4.067446817214517	0.0
15	2.7828727539781863E-5	0.0	0.0	4.180097506295554	0.0
16	2.7828727539781863E-5	0.0	0.0	4.413357900534005	2.7828727539781863E-5
17	2.7828727539781863E-5	0.0	0.0	4.718583384190333	2.7828727539781863E-5
18	2.7828727539781863E-5	0.0	0.0	5.0465449382466625	2.7828727539781863E-5
19	2.7828727539781863E-5	0.0	0.0	5.304851187270917	2.7828727539781863E-5
20	2.7828727539781863E-5	0.0	0.0	5.5538626412968854	2.7828727539781863E-5
21	2.7828727539781863E-5	0.0	0.0	5.851101280149296	2.7828727539781863E-5
22	2.7828727539781863E-5	0.0	0.0	6.176558248727044	2.7828727539781863E-5
23	2.7828727539781863E-5	0.0	0.0	6.503490139864402	2.7828727539781863E-5
24	2.7828727539781863E-5	0.0	0.0	6.730099468220845	2.7828727539781863E-5
25	2.7828727539781863E-5	0.0	0.0	6.937562632029919	2.7828727539781863E-5
26	2.7828727539781863E-5	0.0	0.0	7.1409906303457245	2.7828727539781863E-5
27	2.7828727539781863E-5	0.0	0.0	7.386022576333504	2.7828727539781863E-5
28	2.7828727539781863E-5	0.0	0.0	7.608596739196679	2.7828727539781863E-5
29	2.7828727539781863E-5	0.0	0.0	7.877895335599148	2.7828727539781863E-5
30	2.7828727539781863E-5	0.0	0.0	8.182007670153885	2.7828727539781863E-5
31	2.7828727539781863E-5	0.0	0.0	8.452363758202866	2.7828727539781863E-5
32	2.7828727539781863E-5	0.0	0.0	8.692664820508881	2.7828727539781863E-5
33	2.7828727539781863E-5	0.0	0.0	8.922780568535337	2.7828727539781863E-5
34	2.7828727539781863E-5	0.0	0.0	9.181699049565468	2.7828727539781863E-5
35	2.7828727539781863E-5	0.0	0.0	9.475709556023263	2.7828727539781863E-5
36	2.7828727539781863E-5	0.0	0.0	9.729563208641153	2.7828727539781863E-5
37	2.7828727539781863E-5	0.0	0.0	10.00943672150874	2.7828727539781863E-5
38	2.7828727539781863E-5	0.0	0.0	10.322120304145729	2.7828727539781863E-5
39	5.5657455079563726E-5	0.0	0.0	10.80550530151174	2.7828727539781863E-5
40	5.5657455079563726E-5	0.0	0.0	11.127594994057175	2.7828727539781863E-5
41	5.5657455079563726E-5	0.0	0.0	11.43332139480922	2.7828727539781863E-5
42	5.5657455079563726E-5	0.0	0.0	11.75847224738403	2.7828727539781863E-5
43	5.5657455079563726E-5	0.0	0.0	12.040210284996782	2.7828727539781863E-5
44	5.5657455079563726E-5	0.0	0.0	12.325176455004147	2.7828727539781863E-5
45	5.5657455079563726E-5	0.0	0.0	12.606385746793643	2.7828727539781863E-5
46	5.5657455079563726E-5	0.0	0.0	12.912195633728306	2.7828727539781863E-5
47	8.348618261934558E-5	0.0	0.0	13.235454132830412	2.7828727539781863E-5
48	8.348618261934558E-5	0.0	0.0	13.54067961648674	2.7828727539781863E-5
49	8.348618261934558E-5	0.0	0.0	13.85695310497636	2.7828727539781863E-5
50	8.348618261934558E-5	0.0	0.0	14.196714039509558	2.7828727539781863E-5
51	8.348618261934558E-5	0.0	0.0	14.528933388879473	2.7828727539781863E-5
52	8.348618261934558E-5	0.0	0.0	14.931809877472896	2.7828727539781863E-5
53	8.348618261934558E-5	0.0	0.0	15.268008734881	2.7828727539781863E-5
54	8.348618261934558E-5	0.0	0.0	15.600088940613217	2.7828727539781863E-5
55	8.348618261934558E-5	0.0	0.0	15.926993003023036	2.7828727539781863E-5
56	8.348618261934558E-5	0.0	0.0	16.263609291344235	2.7828727539781863E-5
57	8.348618261934558E-5	0.0	0.0	16.595272066163357	2.7828727539781863E-5
58	8.348618261934558E-5	0.0	0.0	16.91616512342458	2.7828727539781863E-5
59	1.1131491015912745E-4	0.0	0.0	17.249998538991804	2.7828727539781863E-5
60	1.1131491015912745E-4	0.0	0.0	17.623209604027817	2.7828727539781863E-5
61	1.1131491015912745E-4	0.0	0.0	17.99803473526114	2.7828727539781863E-5
62	1.1131491015912745E-4	0.0	0.0	18.42289591861099	2.7828727539781863E-5
63	1.1131491015912745E-4	0.0	0.0	18.81711767293954	2.7828727539781863E-5
64	1.391436376989093E-4	0.0	0.0	19.195449223842875	2.7828727539781863E-5
65	1.6697236523869117E-4	0.0	0.0	19.548122687954532	2.7828727539781863E-5
66	1.6697236523869117E-4	0.0	0.0	19.89756801967157	2.7828727539781863E-5
67	1.6697236523869117E-4	0.0	0.0	20.25586288674626	2.7828727539781863E-5
68	1.6697236523869117E-4	0.0	0.0	20.659435093528177	2.7828727539781863E-5
69	1.9480109277847304E-4	0.0	0.0	21.236547245248175	2.7828727539781863E-5
70	1.9480109277847304E-4	0.0	0.0	21.636613032360078	2.7828727539781863E-5
71	1.9480109277847304E-4	0.0	0.0	22.032476681613478	2.7828727539781863E-5
72	1.9480109277847304E-4	0.0	0.0	22.415566944926113	2.7828727539781863E-5
73	2.504585478580368E-4	0.0	0.0	22.79634742385295	5.5657455079563726E-5
74	2.504585478580368E-4	0.0	0.0	23.208713508537436	5.5657455079563726E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	185	0.0	73.51351	1
CGTTAGG	655	0.0	73.28244	2
TACGGGA	1410	0.0	72.34042	4
CGTTTTT	9930	0.0	71.58106	1
TATAGCG	410	0.0	68.29269	1
CTACGGG	675	0.0	67.55556	3
GCGGGAT	2985	0.0	67.53769	5
CGAACGG	505	0.0	67.32673	2
GCGAATG	310	0.0	67.09678	1
CGGGATT	2235	0.0	66.75616	6
CGTAAGG	625	0.0	66.56	2
CGAGGGA	2545	0.0	66.32613	4
TAGCGGG	2785	0.0	65.637344	3
TCGAACG	385	0.0	65.454544	1
AGCGGGA	3510	0.0	65.07123	4
TAGGGAG	4740	0.0	64.81013	5
TAGGGAC	4395	0.0	63.79977	5
TGCGGGC	410	0.0	63.414635	4
ATCGCGG	265	0.0	63.396225	2
GTACGGG	1375	0.0	62.836365	3
>>END_MODULE