FastQCFastQC Report
Thu 26 May 2016
SRR1547805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547805_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences956522
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70420.7362088901248481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25670.26836810862687943No Hit
GAGAAAGGAAAGAAGGAAGGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAA18300.1913181296405101No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13830.14458632420372977No Hit
TAGATCTGGGGCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAA12240.12796360146447233No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAA11870.1240954207012489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG251.2846394E-680.000011
CTATGCG404.1836756E-1180.01
CCGTATG150.001309723680.080
CTACGCG204.0905496E-580.01
CGGGACG150.001309723680.06
ATACCGG150.001309723680.02
TAGTCGG700.080.02
CGTTAGG1650.077.575762
ACGTTAG900.075.555561
TACGGGA5500.074.909094
CGATAGG2600.073.846152
AGTACGG1300.073.846152
GGTCATA1100.072.727279
CGTTTTT37800.072.169311
GCGATAG1500.072.01
CGAACGG900.071.1111152
GGACCGT850.070.5882348
AGTGCGG2050.070.2439042
TCGTGCA403.7125574E-970.01
TAATGCG403.7125574E-970.01
Produced by FastQC (version 0.11.5)