##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547805_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	956522
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7546475669143	31.0	31.0	33.0	30.0	34.0
2	32.18008158725048	33.0	31.0	34.0	30.0	34.0
3	32.312370232989934	34.0	31.0	34.0	30.0	34.0
4	35.895264301291554	37.0	35.0	37.0	35.0	37.0
5	35.842505452043966	37.0	35.0	37.0	35.0	37.0
6	35.918674113088876	37.0	35.0	37.0	35.0	37.0
7	36.20646258005566	37.0	35.0	37.0	35.0	37.0
8	36.159703592808114	37.0	35.0	37.0	35.0	37.0
9	38.02820008321816	39.0	39.0	39.0	35.0	39.0
10-11	37.46261351019631	39.0	37.5	39.0	34.5	39.0
12-13	36.014790041420895	37.0	35.0	39.0	32.5	39.0
14-15	36.884656599639115	38.0	35.0	41.0	32.5	41.0
16-17	37.20908457934057	38.0	35.0	41.0	33.0	41.0
18-19	37.10561126665147	38.0	35.0	41.0	33.0	41.0
20-21	36.80648693913993	37.0	35.0	41.0	33.0	41.0
22-23	36.5782810013779	37.0	35.0	41.0	32.0	41.0
24-25	36.512766041972895	36.5	35.0	40.5	32.0	41.0
26-27	36.468529735855526	36.5	35.0	40.5	32.5	41.0
28-29	36.52452635694736	36.0	35.0	41.0	32.0	41.0
30-31	36.34767888245121	36.0	35.0	40.0	32.0	41.0
32-33	35.907406207071034	36.0	35.0	40.0	31.0	41.0
34-35	35.63055214621305	36.0	35.0	40.0	30.0	41.0
36-37	35.36312390096621	36.0	35.0	40.0	30.0	41.0
38-39	35.32084625340556	35.5	35.0	40.0	30.0	41.0
40-41	35.1916437886426	36.0	35.0	40.0	29.0	41.0
42-43	35.10927349292541	35.5	35.0	40.0	29.0	41.0
44-45	34.97557662029729	35.0	34.0	40.0	28.0	41.0
46-47	34.87452144331233	35.0	34.0	40.0	28.0	41.0
48-49	34.83856565766391	35.5	34.0	40.0	28.0	41.0
50-51	34.63651646276824	35.0	34.0	40.0	27.5	41.0
52-53	34.45649917095477	35.0	34.0	39.5	27.0	41.0
54-55	34.26866710854533	35.0	34.0	39.0	26.5	41.0
56-57	33.92573458843602	35.0	34.0	39.0	25.5	41.0
58-59	33.62786114694696	35.0	33.0	38.0	24.5	40.0
60-61	33.32765895609302	35.0	33.0	37.5	24.5	40.0
62-63	32.815732413891155	35.0	32.0	37.0	22.5	40.0
64-65	32.615310468551684	35.0	32.0	36.5	23.0	40.0
66-67	32.431978564005846	35.0	32.5	36.0	22.0	39.0
68-69	32.23828516228586	35.0	32.5	36.0	21.0	39.0
70-71	32.054916144113776	35.0	32.0	35.0	20.0	38.5
72-73	31.879866850945405	35.0	32.0	35.0	20.0	37.5
74-75	31.626225533756674	35.0	32.0	35.0	19.5	37.0
76-77	29.58245550023941	32.5	28.5	34.5	16.0	35.5
78-79	30.498284932285927	34.0	30.0	35.0	17.5	36.0
80-81	31.0597910973297	34.5	31.5	35.0	18.5	36.0
82-83	31.01071486071413	34.5	31.5	35.0	18.0	36.0
84-85	30.893751006249726	34.0	31.0	35.0	18.0	35.5
86	30.54426871519944	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	12.0
12	21.0
13	84.0
14	242.0
15	668.0
16	1760.0
17	3578.0
18	6223.0
19	8446.0
20	9736.0
21	10072.0
22	8627.0
23	7745.0
24	8268.0
25	9531.0
26	10928.0
27	13266.0
28	15809.0
29	19548.0
30	24095.0
31	29446.0
32	36496.0
33	51732.0
34	158479.0
35	132736.0
36	67069.0
37	116053.0
38	141675.0
39	63915.0
40	252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48602750381068	23.76704351807904	29.23529202673854	14.51163695137174
2	30.606196198310126	26.100915608841195	30.124659966001825	13.168228226846848
3	28.597564927936837	24.854420494248956	33.495936319290095	13.05207825852411
4	24.884320486094413	27.941646925005386	33.41637725007893	13.75765533882127
5	22.35724844802315	31.872868580126752	32.05352307631189	13.716359895538211
6	22.711030169719045	40.46660714547078	26.921388112348698	9.90097457246148
7	87.24190347948087	4.513644223551575	6.893202665490182	1.3512496314773732
8	87.73859879856396	4.039635261917656	6.281089196066583	1.9406767434517973
9	83.9357589266112	5.982298368464082	8.201588672293997	1.8803540326307182
10-11	51.22213603032654	23.66223672848089	16.3952841649225	8.72034307627007
12-13	34.06335661908456	31.35876644760915	21.37232598936564	13.205550943940652
14-15	12.191983038550081	37.132026236720115	37.28717164895319	13.388819075776615
16-17	15.176911769933154	22.406802979962823	38.61322583275659	23.80305941734743
18-19	23.632964009191635	26.101229245119296	31.558709574897385	18.70709717079168
20-21	28.085030976809733	27.87578330660455	27.038688080357797	17.00049763622792
22-23	20.00894908846843	27.486247049205346	27.340981179732402	25.16382268259381
24-25	16.76286588285476	25.641543006851908	39.84100731608891	17.75458379420442
26-27	14.402177890315121	40.00456863511764	28.64126491601866	16.951988558548575
28-29	13.018100995063364	29.921475930506563	40.11345269633108	16.94697037809899
30-31	23.112484605686017	32.36700253627204	28.015926450201878	16.504586407840073
32-33	29.158451138604235	30.57091211702397	25.366588536384942	14.904048207986854
34-35	18.783990331639	30.9614938286835	28.339808180052316	21.914707659625186
36-37	24.931522745948342	30.509543951942558	29.20973067007345	15.349202632035647
38-39	17.730956527920945	34.90066093618338	27.70035608172107	19.6680264541746
40-41	20.398276255015567	26.823690411720797	27.990940093379972	24.78709323988366
42-43	20.99047382077987	24.890279575378297	27.33016072813798	26.78908587570385
44-45	16.93442492697502	36.72461271146926	26.450567786208783	19.890394575346935
46-47	20.85278749469432	33.60079538160126	25.172499952954556	20.373917170749863
48-49	20.950119286331102	26.401117799695147	32.452625240193115	20.196137673780633
50-51	18.213695032628628	35.829024319357	26.81929950382741	19.13798114418696
52-53	23.205791398420526	26.87402903435572	29.619496467410055	20.3006830998137
54-55	24.158618411285886	28.31393318710913	30.389473530143583	17.1379748714614
56-57	24.778259151383867	24.03347753632431	28.28335364999446	22.904909662297364
58-59	26.813131323691458	27.090751702522265	26.603726835347224	19.492390138439053
60-61	27.110249424477427	30.491300775099788	23.983504822680505	18.41494497774228
62-63	24.378268351381358	25.335695362992173	32.59344792905966	17.692588356566812
64-65	17.710151988140368	32.357332084363975	31.849764040973444	18.082751886522214
66-67	15.78787523967039	29.520282858104675	23.08180052314531	31.61004137907962
68-69	15.850654768003244	35.06124271057017	20.990055639075734	28.098046882350854
70-71	17.74041788897694	32.376359351901996	28.46667405454344	21.416548704577625
72-73	23.973207098216246	31.983320822730683	24.684534176945224	19.358937902107844
74-75	23.30328000819636	35.57158120775058	20.57344211633397	20.551696667719092
76-77	31.433411881796758	28.63248310023188	22.454737057798983	17.47936796017237
78-79	18.20611548924123	29.769571426480518	31.932093563974483	20.092219520303768
80-81	13.964864373218807	28.808955779375694	33.1702773171971	24.0559025302084
82-83	18.115004150453416	24.904184117040696	34.1399884163668	22.840823316139094
84-85	14.390259711747353	25.928363383173625	30.509439406516524	29.1719374985625
86	16.695799992054546	25.356761266337834	34.58979511187406	23.357643629733555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1724.0
1	1423.0
2	2812.5
3	2903.0
4	1369.5
5	1866.0
6	2596.5
7	2897.0
8	3159.5
9	3160.0
10	2676.0
11	2408.0
12	2498.5
13	2619.5
14	2604.0
15	2659.0
16	2754.5
17	2864.0
18	2968.5
19	3147.0
20	3342.0
21	3394.0
22	3183.5
23	3651.0
24	4511.5
25	5208.0
26	5891.0
27	6440.5
28	7572.0
29	8323.0
30	10940.5
31	14458.0
32	14679.0
33	15945.0
34	18326.0
35	21180.5
36	23599.0
37	22442.5
38	23764.5
39	26977.0
40	31877.5
41	41286.5
42	51535.0
43	56541.0
44	58158.0
45	61807.0
46	64393.5
47	69874.0
48	68315.5
49	53586.0
50	45341.0
51	36700.5
52	25758.0
53	21835.0
54	19390.0
55	18926.0
56	19173.0
57	19060.0
58	16340.5
59	11871.0
60	8627.0
61	6870.5
62	6077.0
63	4333.5
64	3121.0
65	2560.5
66	1537.5
67	842.5
68	585.5
69	388.0
70	193.0
71	171.0
72	116.0
73	75.0
74	60.0
75	29.0
76	27.5
77	23.0
78	41.5
79	65.0
80	35.5
81	3.5
82	1.5
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	1.0
89	1.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	956522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.78537375084402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20777074908342	15.869407219926298
2	9.963244854195189	3.9425304642285046
3	3.198028488645798	1.8982256674111186
4	1.3810804754597612	1.0930077354785896
5	0.7012593076797008	0.6937338749350501
6	0.4181914544036792	0.49644445348715033
7	0.2720198046843511	0.3767409452318035
8	0.2081353459451559	0.32944284882289016
9	0.16895769118623055	0.3008601961379331
>10	1.3187787533914235	5.814111593286219
>50	0.6908387681519622	10.088978320501553
>100	1.4605087912665216	56.51488735432264
>500	0.007989654219182284	0.9762915490956852
>1k	0.002663218073060761	0.8632128755011612
>5k	5.326436146121523E-4	0.7421249016333997
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7042	0.7362088901248481	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2567	0.26836810862687943	No Hit
GAGAAAGGAAAGAAGGAAGGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1830	0.1913181296405101	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1383	0.14458632420372977	No Hit
TAGATCTGGGGCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAA	1224	0.12796360146447233	No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAA	1187	0.1240954207012489	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0454542603306563E-4	0.0	0.0	0.05812725687438449	0.0
2	1.0454542603306563E-4	0.0	0.0	0.1797135873508398	0.0
3	1.0454542603306563E-4	0.0	0.0	0.254881748668614	0.0
4	1.0454542603306563E-4	0.0	0.0	0.42173624861738673	0.0
5	1.0454542603306563E-4	0.0	0.0	0.6686725449074877	0.0
6	1.0454542603306563E-4	0.0	0.0	0.9198952036649445	0.0
7	1.0454542603306563E-4	0.0	0.0	1.0627042556261121	0.0
8	1.0454542603306563E-4	0.0	0.0	1.3421541794124965	0.0
9	1.0454542603306563E-4	0.0	0.0	1.432063245800933	0.0
10	1.0454542603306563E-4	0.0	0.0	1.5756041157443321	0.0
11	1.0454542603306563E-4	0.0	0.0	1.8386404076435252	0.0
12	1.0454542603306563E-4	0.0	0.0	2.044072169798499	0.0
13	1.0454542603306563E-4	0.0	0.0	2.1148494232228847	0.0
14	1.0454542603306563E-4	0.0	0.0	2.1440175970861097	0.0
15	1.0454542603306563E-4	0.0	0.0	2.190226675392725	0.0
16	1.0454542603306563E-4	0.0	0.0	2.291322102366699	0.0
17	1.0454542603306563E-4	0.0	0.0	2.4034993445001787	0.0
18	1.0454542603306563E-4	0.0	0.0	2.554776575970025	0.0
19	1.0454542603306563E-4	0.0	0.0	2.642281097559701	0.0
20	1.0454542603306563E-4	0.0	0.0	2.72853107403698	0.0
21	1.0454542603306563E-4	0.0	0.0	2.8412310433006245	0.0
22	1.0454542603306563E-4	0.0	0.0	2.9609355561084847	0.0
23	1.0454542603306563E-4	0.0	0.0	3.0945446105787426	0.0
24	1.0454542603306563E-4	0.0	0.0	3.176508224588666	0.0
25	1.0454542603306563E-4	0.0	0.0	3.255962748373796	0.0
26	1.0454542603306563E-4	0.0	0.0	3.3299809100052062	0.0
27	1.0454542603306563E-4	0.0	0.0	3.4051490713229806	0.0
28	1.0454542603306563E-4	0.0	0.0	3.4860672310725733	0.0
29	1.0454542603306563E-4	0.0	0.0	3.5783808422597705	0.0
30	1.0454542603306563E-4	0.0	0.0	3.697248991659366	0.0
31	1.0454542603306563E-4	0.0	0.0	3.8144444142424323	0.0
32	1.0454542603306563E-4	0.0	0.0	3.910312569914754	0.0
33	1.0454542603306563E-4	0.0	0.0	4.013185269131291	0.0
34	1.0454542603306563E-4	0.0	0.0	4.123794329874274	0.0
35	1.0454542603306563E-4	0.0	0.0	4.261480655959821	0.0
36	1.0454542603306563E-4	0.0	0.0	4.375121534057763	0.0
37	1.0454542603306563E-4	0.0	0.0	4.4920033203627305	0.0
38	1.0454542603306563E-4	0.0	0.0	4.611603287744558	0.0
39	1.0454542603306563E-4	0.0	0.0	4.740716888895394	0.0
40	1.0454542603306563E-4	0.0	0.0	4.884571395116892	0.0
41	1.0454542603306563E-4	0.0	0.0	5.029262264746655	0.0
42	1.0454542603306563E-4	0.0	0.0	5.17165313500369	0.0
43	1.0454542603306563E-4	0.0	0.0	5.299616736468163	0.0
44	1.0454542603306563E-4	0.0	0.0	5.436675789997512	0.0
45	1.0454542603306563E-4	0.0	0.0	5.577812115142151	0.0
46	1.0454542603306563E-4	0.0	0.0	5.724280257014476	0.0
47	1.0454542603306563E-4	0.0	0.0	5.885593849383496	0.0
48	1.0454542603306563E-4	0.0	0.0	6.02558017484177	0.0
49	1.0454542603306563E-4	0.0	0.0	6.204666489636412	0.0
50	1.0454542603306563E-4	0.0	0.0	6.377898260573201	0.0
51	1.0454542603306563E-4	0.0	0.0	6.5213345850905675	0.0
52	1.0454542603306563E-4	0.0	0.0	6.692893629210828	0.0
53	1.0454542603306563E-4	0.0	0.0	6.8652890367393535	0.0
54	1.0454542603306563E-4	0.0	0.0	7.066434436426972	0.0
55	1.0454542603306563E-4	0.0	0.0	7.2616207468307055	0.0
56	1.0454542603306563E-4	0.0	0.0	7.448443423151794	0.0
57	1.0454542603306563E-4	0.0	0.0	7.633593372656353	0.0
58	2.0909085206613127E-4	0.0	0.0	7.806825143593143	0.0
59	2.0909085206613127E-4	0.0	0.0	7.978384187713403	0.0
60	2.0909085206613127E-4	0.0	0.0	8.1750341340816	0.0
61	3.136362780991969E-4	0.0	0.0	8.393534074490708	0.0
62	3.136362780991969E-4	0.0	0.0	8.637543098851882	0.0
63	3.136362780991969E-4	0.0	0.0	8.925774838425044	0.0
64	4.1818170413226253E-4	0.0	0.0	9.163092955520103	0.0
65	4.1818170413226253E-4	0.0	0.0	9.378979260278383	0.0
66	4.1818170413226253E-4	0.0	0.0	9.55074739525071	0.0
67	4.1818170413226253E-4	0.0	0.0	9.752729158346593	0.0
68	4.1818170413226253E-4	0.0	0.0	10.00091999974909	0.0
69	4.1818170413226253E-4	0.0	0.0	10.25935629290283	0.0
70	4.1818170413226253E-4	0.0	0.0	10.475556233939209	0.0
71	4.1818170413226253E-4	0.0	0.0	10.682974359188812	0.0
72	4.1818170413226253E-4	0.0	0.0	10.902728844710316	0.0
73	4.1818170413226253E-4	0.0	0.0	11.139837870953308	0.0
74	4.1818170413226253E-4	0.0	0.0	11.39869234581118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	25	1.2846394E-6	80.00001	1
CTATGCG	40	4.1836756E-11	80.0	1
CCGTATG	15	0.0013097236	80.0	80
CTACGCG	20	4.0905496E-5	80.0	1
CGGGACG	15	0.0013097236	80.0	6
ATACCGG	15	0.0013097236	80.0	2
TAGTCGG	70	0.0	80.0	2
CGTTAGG	165	0.0	77.57576	2
ACGTTAG	90	0.0	75.55556	1
TACGGGA	550	0.0	74.90909	4
CGATAGG	260	0.0	73.84615	2
AGTACGG	130	0.0	73.84615	2
GGTCATA	110	0.0	72.72727	9
CGTTTTT	3780	0.0	72.16931	1
GCGATAG	150	0.0	72.0	1
CGAACGG	90	0.0	71.111115	2
GGACCGT	85	0.0	70.588234	8
AGTGCGG	205	0.0	70.243904	2
TCGTGCA	40	3.7125574E-9	70.0	1
TAATGCG	40	3.7125574E-9	70.0	1
>>END_MODULE