FastQCFastQC Report
Thu 26 May 2016
SRR1547804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547804_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2669130
Sequences flagged as poor quality0
Sequence length86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1374645.1501425558140665No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC35320.13232776222964038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33210.12442256465589911No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG28340.10617691907100815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT465100.077.540321
TACGGGA10350.066.473434
ATAGGGA33900.064.542774
TAGGGAC23450.063.4541635
TAGGGAT38200.063.0366485
TATCGCG900.062.222221
GTAGGGA35900.062.061284
TATAGCG1550.061.9354861
CGAGGGA16650.061.261264
AGTAGGG36800.061.1956523
GTACGGG8350.060.838323
ACGGGAT12150.060.2469145
AGGGATC27550.059.818516
GTTTTTT611750.059.5145072
AGGGATT43150.059.5133256
GAGGGAT41400.059.323675
GCGTAAG1300.058.461541
TAGGGGT27850.058.1687665
TATGGGA27650.057.8661884
ATAGCGG5200.057.692312
Produced by FastQC (version 0.11.5)