##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547804_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2669130
Sequences flagged as poor quality	0
Sequence length	86
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841594077470937	31.0	31.0	33.0	30.0	34.0
2	32.28455376845639	33.0	31.0	34.0	31.0	34.0
3	32.202460726903524	33.0	31.0	34.0	30.0	34.0
4	35.95011932727143	37.0	35.0	37.0	35.0	37.0
5	35.95779785922754	37.0	35.0	37.0	35.0	37.0
6	36.021478159550114	37.0	35.0	37.0	35.0	37.0
7	36.27050162412471	37.0	35.0	37.0	35.0	37.0
8	36.22689565513856	37.0	37.0	37.0	35.0	37.0
9	38.080699703648754	39.0	39.0	39.0	37.0	39.0
10-11	37.656865345636966	39.0	37.5	39.0	35.0	39.0
12-13	37.24999344355651	39.0	37.0	39.0	34.0	39.0
14-15	38.52282391640722	40.0	38.0	41.0	34.0	41.0
16-17	38.55951901930592	40.0	38.0	41.0	34.0	41.0
18-19	38.06180553213969	39.0	37.0	41.0	34.0	41.0
20-21	37.52682859208806	39.0	35.0	41.0	33.5	41.0
22-23	37.33976464241157	39.0	35.0	41.0	33.0	41.0
24-25	37.208775143960764	39.0	35.0	41.0	33.0	41.0
26-27	37.10696556555881	39.0	35.0	41.0	33.0	41.0
28-29	36.934693701693064	38.5	35.0	41.0	33.0	41.0
30-31	36.4862383997782	38.0	35.0	41.0	31.5	41.0
32-33	35.60265273703416	38.0	35.0	41.0	27.0	41.0
34-35	34.7762696084492	38.0	35.0	41.0	21.0	41.0
36-37	34.43363080854061	38.0	34.0	41.0	18.0	41.0
38-39	34.24235631085784	38.0	34.0	41.0	18.0	41.0
40-41	34.04735681664063	38.0	34.0	40.0	17.5	41.0
42-43	33.88913784641437	38.0	33.5	40.0	15.5	41.0
44-45	33.74224728656903	37.0	33.0	40.0	15.0	41.0
46-47	33.68048521428331	37.0	33.0	40.0	16.5	41.0
48-49	33.560097859602195	36.5	33.0	40.0	17.0	41.0
50-51	33.37382892553004	36.0	33.0	40.0	15.0	41.0
52-53	33.14374402895325	36.0	33.0	40.0	15.0	41.0
54-55	32.908239950845406	35.0	33.0	40.0	13.5	41.0
56-57	32.60663474615324	35.0	32.5	39.5	11.0	41.0
58-59	32.28581653947166	35.0	32.0	39.0	10.0	41.0
60-61	31.94295912900458	35.0	31.0	39.0	10.0	41.0
62-63	31.61269739578065	35.0	31.0	38.5	10.0	40.0
64-65	31.30512826276727	35.0	31.0	38.0	10.0	40.0
66-67	30.953219026424343	35.0	30.0	37.0	9.0	40.0
68-69	30.45467980203287	35.0	29.0	36.5	8.0	39.5
70-71	30.15036247766126	35.0	29.0	36.0	8.0	39.0
72-73	29.895480737169038	35.0	29.0	36.0	7.5	38.5
74-75	29.614136254135243	35.0	29.0	35.0	7.0	37.5
76-77	27.711559759172466	32.0	24.5	34.5	7.0	35.5
78-79	28.867895344175817	34.0	27.0	35.0	7.0	36.0
80-81	28.964249399617103	34.0	27.0	35.0	7.0	36.0
82-83	28.792580541225043	34.0	27.0	35.0	7.0	36.0
84-85	28.56423197821013	34.0	26.0	35.0	7.0	35.5
86	28.17819401827562	34.0	25.0	35.0	7.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	19.0
11	26.0
12	79.0
13	177.0
14	724.0
15	2370.0
16	6358.0
17	15517.0
18	34475.0
19	56614.0
20	68012.0
21	67225.0
22	56047.0
23	42824.0
24	35008.0
25	32094.0
26	32730.0
27	36017.0
28	41166.0
29	49543.0
30	60788.0
31	73388.0
32	83993.0
33	106765.0
34	212471.0
35	252510.0
36	238587.0
37	361063.0
38	478153.0
39	223529.0
40	846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.945184385923504	21.35190118128379	25.184910438981987	26.51800399381072
2	41.393300438719734	21.719848789680533	25.713209922334247	11.17364084926549
3	24.435939800609187	21.57508251752444	42.80567076163394	11.183306920232436
4	22.733137763990513	23.198570320666285	42.06329403213781	12.00499788320539
5	20.79909933199207	26.69772547609146	40.33711359132002	12.16606160059645
6	19.367734055666077	34.27277802130282	37.08212039128855	9.277367531742552
7	74.66462854937751	3.2524455534200283	20.504771217587752	1.578154679614706
8	75.24399336113265	3.0037128202822645	20.179646551498053	1.5726472670870284
9	71.30742976175758	5.21064916283583	21.14014678940329	2.341774286003304
10-11	29.750798949470425	27.351271762709196	31.968412928557242	10.92951635926313
12-13	22.966135032763486	21.53594991626485	39.26841330321116	16.229501747760505
14-15	15.196056392907051	26.614833297741214	42.1765331774773	16.01257713187443
16-17	20.02669409133313	27.230052489013275	39.09288794476103	13.650365474892567
18-19	19.36119634487642	28.290959975722423	38.34906879769812	13.998774881703028
20-21	21.368779340084597	27.6633772053066	38.68043145144673	12.287412003162078
22-23	19.373691052889892	23.584332722647456	41.253329736655765	15.788646487806886
24-25	17.399283661717487	26.750888866409657	40.41588457662235	15.433942895250514
26-27	16.336540370832445	30.094000666883964	38.156777676621225	15.41268128566237
28-29	15.649181568526075	28.24939212402543	40.48347214260827	15.617954164840228
30-31	18.567004979150507	27.390741552490887	38.56685511758513	15.475398350773473
32-33	20.362496393956082	28.48978880758899	35.89349338548516	15.254221412969768
34-35	18.246001506108733	30.14264947754512	35.67576326368517	15.93558575266098
36-37	19.467672987078185	31.66520551640422	33.48930175750151	15.377819739016083
38-39	18.63189503695961	32.13442207760581	32.66292762061046	16.57075526482412
40-41	18.677040833530025	30.407136407743344	32.84444744167575	18.071375317050876
42-43	18.501646603949602	30.154245016166314	32.47944086649957	18.86466751338451
44-45	18.308156590349665	31.057104749487664	32.65187533016376	17.982863329998914
46-47	18.1656757070656	31.088369618564847	32.979697504430284	17.766257169939266
48-49	18.40878863150165	29.239677348049735	34.862539479156126	17.48899454129248
50-51	17.11570811462911	30.176742983668838	34.347727536687984	18.359821365014067
52-53	17.99421534357637	28.951212567390872	34.647975182924775	18.406596906107982
54-55	17.860314034910253	29.346547376860627	34.51864465200271	18.27449393622641
56-57	18.52628009875877	30.32473127948058	32.69112032759738	18.457868294163266
58-59	19.30610348690397	31.629894385061796	31.803546473944692	17.260455654089533
60-61	20.02197345202369	31.903897524661595	31.046951628433234	17.02717739488148
62-63	18.942052279207083	31.19456527033153	32.07449618414989	17.788886266311497
64-65	18.521802984493075	33.24967311446051	30.824819323150237	17.403704577896168
66-67	18.373065380854435	33.20793666850247	29.689449371143407	18.72954857949969
68-69	17.74012505947631	33.82253768081734	29.99962534608655	18.437711913619793
70-71	18.078456276015032	33.19920723231915	30.558253063732376	18.16408342793345
72-73	18.75169062578443	34.11858920322352	29.44513755418432	17.684582616807724
74-75	18.012217464117523	34.1053826527745	29.391412182995957	18.490987700112022
76-77	19.164147119098732	32.36164967611169	29.459955116461167	19.014248088328404
78-79	18.148853746351808	33.21276970398594	29.68993642123089	18.94844012843136
80-81	17.589139532356985	33.1972028338822	29.77024348757835	19.443414146182462
82-83	18.06613016226261	33.145893980435574	29.52366501444291	19.264310842858908
84-85	17.692562745164157	32.669240539052055	29.19902739844069	20.439169317343104
86	17.833338953142036	32.907052110612824	29.709343493947465	19.550265442297675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2663.0
1	3282.0
2	46433.5
3	53014.0
4	17541.0
5	20371.5
6	25320.0
7	27917.0
8	29455.5
9	30897.0
10	29921.5
11	28295.5
12	27585.0
13	26870.0
14	26118.0
15	25779.5
16	24805.5
17	23823.0
18	23447.5
19	23023.5
20	22975.0
21	23322.0
22	23613.5
23	24290.5
24	26007.5
25	28187.5
26	31172.0
27	35596.0
28	39705.5
29	41527.0
30	44770.0
31	50326.5
32	51922.5
33	54577.0
34	61010.0
35	66659.5
36	69248.0
37	70925.5
38	75033.0
39	78768.5
40	82342.0
41	87332.5
42	91884.5
43	93714.0
44	94708.5
45	94602.0
46	94841.5
47	93211.0
48	88304.5
49	82858.5
50	79348.0
51	75875.5
52	70539.5
53	67839.0
54	66179.5
55	63127.0
56	56287.0
57	51677.0
58	47659.5
59	38444.5
60	30243.5
61	24456.0
62	19912.0
63	15123.0
64	12094.0
65	10455.0
66	7715.5
67	5764.5
68	4265.0
69	3192.5
70	2104.5
71	1440.0
72	1074.5
73	659.5
74	513.5
75	344.0
76	212.0
77	115.5
78	61.5
79	45.0
80	45.0
81	35.0
82	22.0
83	13.0
84	5.0
85	4.5
86	6.0
87	4.5
88	3.0
89	2.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.5
95	1.5
96	2.5
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2669130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.284047060303777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94009707905526	18.44132891231815
2	6.678790561041624	3.243761285804949
3	3.0995409354197156	2.258081938232111
4	2.0095164303765025	1.951967662548666
5	1.4715747439174833	1.786789516702332
6	1.1147161865788997	1.6241892200258619
7	0.8681948603459475	1.4758299392308396
8	0.645943061224331	1.2548889357638668
9	0.550789724827081	1.203786323822934
>10	6.254228523905428	35.17609401950959
>50	1.2315830182195704	20.11869457126205
>100	0.12829028800887676	4.335899149162233
>500	0.003758839300026952	0.616823454292728
>1k	0.002819129475020214	1.283660649021696
>5k	0.0	0.0
>10k+	1.566183041677897E-4	5.228204422302107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137464	5.1501425558140665	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	3532	0.13232776222964038	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3321	0.12442256465589911	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	2834	0.10617691907100815	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7465391344745294E-5	0.0	0.0	0.03263235586127315	0.0
2	3.7465391344745294E-5	0.0	0.0	0.1026551722846021	0.0
3	3.7465391344745294E-5	0.0	0.0	0.15653040503834584	0.0
4	3.7465391344745294E-5	0.0	0.0	0.2110050840536055	0.0
5	3.7465391344745294E-5	0.0	0.0	0.38533154998070535	0.0
6	3.7465391344745294E-5	0.0	0.0	0.6351882448588116	0.0
7	3.7465391344745294E-5	0.0	0.0	0.8336424228119275	0.0
8	3.7465391344745294E-5	0.0	0.0	1.0926032077868069	0.0
9	3.7465391344745294E-5	0.0	0.0	1.3514515965876521	0.0
10	3.7465391344745294E-5	0.0	0.0	1.6139341283489377	0.0
11	3.7465391344745294E-5	0.0	0.0	1.9345254820859232	0.0
12	3.7465391344745294E-5	0.0	0.0	2.138224814827303	0.0
13	3.7465391344745294E-5	0.0	0.0	2.230801796840169	0.0
14	3.7465391344745294E-5	0.0	0.0	2.291495730818656	0.0
15	3.7465391344745294E-5	0.0	0.0	2.343272901657094	0.0
16	3.7465391344745294E-5	0.0	0.0	2.4114599139045305	0.0
17	3.7465391344745294E-5	0.0	0.0	2.4878143814651215	0.0
18	3.7465391344745294E-5	0.0	0.0	2.5710999464244906	0.0
19	3.7465391344745294E-5	0.0	0.0	2.6340418038836626	0.0
20	3.7465391344745294E-5	0.0	0.0	2.703240381697407	0.0
21	3.7465391344745294E-5	0.0	0.0	2.7823298228261644	0.0
22	3.7465391344745294E-5	0.0	0.0	2.878278690060057	0.0
23	3.7465391344745294E-5	0.0	0.0	2.971979633813265	0.0
24	3.7465391344745294E-5	0.0	0.0	3.0529423445092596	0.0
25	3.7465391344745294E-5	0.0	0.0	3.120117791190388	0.0
26	3.7465391344745294E-5	0.0	0.0	3.187630426393619	0.0
27	3.7465391344745294E-5	0.0	0.0	3.257503381251569	0.0
28	3.7465391344745294E-5	0.0	0.0	3.3324716293324044	0.0
29	3.7465391344745294E-5	0.0	0.0	3.4171434137715284	0.0
30	7.493078268949059E-5	0.0	0.0	3.5121931116131475	0.0
31	7.493078268949059E-5	0.0	0.0	3.5977266000532007	0.0
32	7.493078268949059E-5	0.0	0.0	3.680038064837606	0.0
33	7.493078268949059E-5	0.0	0.0	3.7619374103172194	0.0
34	7.493078268949059E-5	0.0	0.0	3.8533529651983978	0.0
35	7.493078268949059E-5	0.0	0.0	3.949151970866912	0.0
36	1.1239617403423587E-4	0.0	0.0	4.0537178781100955	0.0
37	1.1239617403423587E-4	0.0	0.0	4.161505809008928	0.0
38	1.1239617403423587E-4	0.0	0.0	4.290649012974265	0.0
39	1.1239617403423587E-4	0.0	0.0	4.438599843394664	0.0
40	1.1239617403423587E-4	0.0	0.0	4.569091801448412	0.0
41	1.1239617403423587E-4	0.0	0.0	4.701569425243432	0.0
42	1.1239617403423587E-4	0.0	0.0	4.831874056340456	0.0
43	1.1239617403423587E-4	0.0	0.0	4.951575981686917	0.0
44	1.1239617403423587E-4	0.0	0.0	5.064496671199979	0.0
45	1.1239617403423587E-4	0.0	0.0	5.177379895321697	0.0
46	1.1239617403423587E-4	0.0	0.0	5.303188679457351	0.0
47	1.1239617403423587E-4	0.0	0.0	5.43731478047154	0.0
48	1.1239617403423587E-4	0.0	0.0	5.577435344100887	0.0
49	1.1239617403423587E-4	0.0	0.0	5.712198356767936	0.0
50	1.1239617403423587E-4	0.0	0.0	5.84905943135029	0.0
51	1.1239617403423587E-4	0.0	0.0	5.980263231839588	0.0
52	1.4986156537898117E-4	0.0	0.0	6.121732549557346	0.0
53	1.4986156537898117E-4	0.0	0.0	6.2602795667502145	0.0
54	1.4986156537898117E-4	0.0	0.0	6.40069985351032	0.0
55	1.8732695672372645E-4	0.0	0.0	6.538160374354191	0.0
56	1.8732695672372645E-4	0.0	0.0	6.674721725805787	0.0
57	1.8732695672372645E-4	0.0	0.0	6.819000947874401	0.0
58	1.8732695672372645E-4	0.0	0.0	6.960470265592159	0.0
59	2.2479234806847175E-4	0.0	0.0	7.111418327320138	0.0
60	2.2479234806847175E-4	0.0	0.0	7.282372908026211	0.0
61	2.2479234806847175E-4	0.0	0.0	7.461007893957956	0.0
62	2.2479234806847175E-4	0.0	0.0	7.668079111920363	0.0
63	2.2479234806847175E-4	0.0	0.0	7.856305238036364	0.0
64	2.2479234806847175E-4	0.0	0.0	8.026922630220334	0.0
65	2.62257739413217E-4	0.0	0.0	8.183790223780782	0.0
66	2.62257739413217E-4	0.0	0.0	8.339983440297026	0.0
67	3.371885221027076E-4	0.0	0.0	8.503444942734149	0.0
68	3.746539134474529E-4	0.0	0.0	8.683503613536995	0.0
69	3.746539134474529E-4	0.0	0.0	8.90121500264131	0.0
70	4.121193047921982E-4	0.0	0.0	9.094461491197507	0.0
71	4.121193047921982E-4	0.0	0.0	9.273546061825389	0.0
72	4.121193047921982E-4	0.0	0.0	9.45135680914755	0.0
73	4.495846961369435E-4	0.0	0.0	9.627219356119785	0.0
74	4.495846961369435E-4	0.0	0.0	9.819341882935639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	46510	0.0	77.54032	1
TACGGGA	1035	0.0	66.47343	4
ATAGGGA	3390	0.0	64.54277	4
TAGGGAC	2345	0.0	63.454163	5
TAGGGAT	3820	0.0	63.036648	5
TATCGCG	90	0.0	62.22222	1
GTAGGGA	3590	0.0	62.06128	4
TATAGCG	155	0.0	61.935486	1
CGAGGGA	1665	0.0	61.26126	4
AGTAGGG	3680	0.0	61.195652	3
GTACGGG	835	0.0	60.83832	3
ACGGGAT	1215	0.0	60.246914	5
AGGGATC	2755	0.0	59.81851	6
GTTTTTT	61175	0.0	59.514507	2
AGGGATT	4315	0.0	59.513325	6
GAGGGAT	4140	0.0	59.32367	5
GCGTAAG	130	0.0	58.46154	1
TAGGGGT	2785	0.0	58.168766	5
TATGGGA	2765	0.0	57.866188	4
ATAGCGG	520	0.0	57.69231	2
>>END_MODULE