FastQCFastQC Report
Thu 26 May 2016
SRR1547803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547803_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2818795
Sequences flagged as poor quality0
Sequence length86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253320.8986818835708167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA7150.072.727276
ACGGGAT20750.071.7108465
CGTTTTT107150.071.376571
ACGGGTA2200.070.909095
TACGGGA18350.070.844694
CGAGGGA23300.068.84124
AGTACGG4250.068.705882
GCGCGAG1900.067.3684161
GGCGCGA1200.066.666678
CAGCGCG1450.066.2068941
TATAGCG1150.066.086961
ACGGGTC1700.065.8823555
TACGCGG1700.065.8823552
TAGGGAC32850.065.875195
TTAGGGA45600.065.614034
GGGCGAT17250.065.1594247
TAGCGAG3200.065.01
AGGGCGA9850.064.974626
TTGCGGG23050.064.902393
CGCGAGG4500.064.8888852
Produced by FastQC (version 0.11.5)