##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547803_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2818795
Sequences flagged as poor quality	0
Sequence length	86
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.836114013257436	31.0	31.0	33.0	30.0	34.0
2	32.28672677509361	33.0	31.0	34.0	31.0	34.0
3	32.433261021110084	34.0	31.0	34.0	31.0	34.0
4	36.02712400156805	37.0	35.0	37.0	35.0	37.0
5	35.98475447842075	37.0	35.0	37.0	35.0	37.0
6	36.03760578545088	37.0	35.0	37.0	35.0	37.0
7	36.30429350130109	37.0	36.0	37.0	35.0	37.0
8	36.270995584992875	37.0	37.0	37.0	35.0	37.0
9	38.149223338341386	39.0	39.0	39.0	37.0	39.0
10-11	37.60350504382192	39.0	37.5	39.0	35.0	39.0
12-13	37.04429002463819	39.0	35.0	39.0	33.5	39.0
14-15	38.22097616179963	40.0	37.0	41.0	33.0	41.0
16-17	38.32507099664927	40.0	36.5	41.0	34.0	41.0
18-19	38.20498599578898	40.0	36.0	41.0	34.0	41.0
20-21	38.05197274012477	40.0	35.5	41.0	34.0	41.0
22-23	37.85981208282262	40.0	35.0	41.0	34.0	41.0
24-25	37.78043454738638	40.0	35.0	41.0	33.5	41.0
26-27	37.73835220368987	40.0	35.0	41.0	34.0	41.0
28-29	37.700371967454174	40.0	35.0	41.0	34.0	41.0
30-31	37.52331705569223	40.0	35.0	41.0	33.0	41.0
32-33	37.22363545415683	40.0	35.0	41.0	33.0	41.0
34-35	36.95190764138577	39.5	35.0	41.0	32.0	41.0
36-37	36.7811291704434	39.0	35.0	41.0	31.5	41.0
38-39	36.659751950744905	39.0	35.0	41.0	31.0	41.0
40-41	36.53560918761386	39.0	35.0	41.0	31.0	41.0
42-43	36.48185040061445	39.0	35.0	41.0	31.0	41.0
44-45	36.374122453034005	39.0	35.0	41.0	31.0	41.0
46-47	36.27845710667147	39.0	35.0	41.0	31.0	41.0
48-49	36.16570289786948	38.0	35.0	41.0	30.0	41.0
50-51	36.012499490030315	38.0	35.0	40.0	30.0	41.0
52-53	35.83401825957546	38.0	35.0	40.0	29.5	41.0
54-55	35.65210258284125	38.0	35.0	40.0	29.0	41.0
56-57	35.37424892551604	37.0	35.0	40.0	28.5	41.0
58-59	35.061017385088306	37.0	34.0	40.0	28.0	41.0
60-61	34.73566009589204	36.0	34.0	40.0	26.5	41.0
62-63	34.3308215035148	36.0	34.0	39.0	26.0	41.0
64-65	33.928971954328006	35.0	34.0	39.0	25.0	40.5
66-67	33.561058360043916	35.0	33.0	38.5	24.0	40.0
68-69	33.03094265457403	35.0	33.0	37.5	21.0	40.0
70-71	32.64582720630624	35.0	33.0	37.0	20.0	39.5
72-73	32.29911149977207	35.0	33.0	37.0	19.5	39.0
74-75	31.926430088034074	35.0	32.0	36.0	18.5	39.0
76-77	29.875531388412426	33.0	29.0	34.5	14.0	36.0
78-79	30.914214052458586	34.0	31.0	35.0	15.0	37.0
80-81	30.99021833797775	35.0	31.0	35.0	13.5	37.0
82-83	30.75624495573463	35.0	31.0	35.0	11.0	36.0
84-85	30.490479265076033	34.5	31.0	35.0	10.0	36.0
86	30.061738083117078	34.0	30.0	35.0	10.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	20.0
11	20.0
12	34.0
13	77.0
14	329.0
15	1021.0
16	2573.0
17	6235.0
18	13156.0
19	20024.0
20	21830.0
21	21218.0
22	18786.0
23	17374.0
24	18673.0
25	21756.0
26	26357.0
27	32882.0
28	41247.0
29	52512.0
30	66683.0
31	83491.0
32	102264.0
33	128230.0
34	270001.0
35	274423.0
36	253053.0
37	401097.0
38	594454.0
39	327819.0
40	1148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.49849137663434	24.511679636156586	31.952483241952677	14.037345745256394
2	29.260056158748686	25.602926073020566	31.97834535679253	13.158672411438221
3	28.239974882884354	24.747950808767577	34.06196619477472	12.950108113573352
4	25.403656526991142	27.78336132992999	33.10574199258903	13.707240150489838
5	23.970668317490276	31.209612618157763	31.06742420076664	13.752294863585327
6	21.437848442330854	40.789096049907855	27.311705888509096	10.461349619252198
7	89.09707871625996	2.888149014029044	6.569615740059138	1.4451565296518547
8	89.571288440628	2.5172813205642837	6.265691545500826	1.6457386933068918
9	84.90883515828573	5.432569590906753	7.534850884863922	2.1237443659436037
10-11	35.320571378904816	31.613739204163483	21.508162175681452	11.55752724125025
12-13	26.981582555666517	25.953430455212246	30.318292036136008	16.74669495298523
14-15	15.708325720742375	32.890277583151665	34.54249067420653	16.85890602189943
16-17	20.374965189025808	28.913046177533307	32.48859885163696	18.223389781803927
18-19	21.000125940339757	30.502448741394815	30.957465867507217	17.539959450758214
20-21	23.608155257831804	30.395204333766735	30.233025104698996	15.763615303702469
22-23	19.976177763902662	27.236212636960122	32.023187212975756	20.764422386161463
24-25	18.57948165794249	30.412037767911464	32.957096915525966	18.051383658620086
26-27	17.248948575543803	34.86856972571613	29.963441825318977	17.919039873421088
28-29	15.768706131520737	31.373547916751658	34.885208041024626	17.972537910702975
30-31	20.585250080264792	30.53590275277202	31.435258683231666	17.443588483731524
32-33	22.480581241275083	30.119164394714765	30.058180889351654	17.3420734746585
34-35	18.03066558582657	30.33118761740389	30.90689461276893	20.73125218400061
36-37	20.092291209541667	30.832057670032764	31.032409238699515	18.043241881726058
38-39	18.670744059074888	31.662217365931188	29.611003992840914	20.05603458215301
40-41	18.71469901145702	29.19254504140954	29.835709939885664	22.257046007247776
42-43	19.239887966311848	29.657619656626323	29.928480077479918	21.17401229958191
44-45	18.567792265844094	32.3079720235065	28.795584638116644	20.328651072532764
46-47	19.88087108143728	31.218694513080948	28.979439796083078	19.920994609398697
48-49	19.48924983902696	29.05308828772578	31.33808950278399	20.119572370463263
50-51	18.046097711965576	30.847436581943704	30.080176103618744	21.02628960247198
52-53	19.337110360987587	28.690717132675488	31.114359149920446	20.857813356416482
54-55	19.338830954361704	28.80498581840822	31.80674720935719	20.049436017872885
56-57	19.83203815814914	28.4569647668596	30.636264786903624	21.07473228808764
58-59	20.446822134990306	30.210799295443618	29.82442852353577	19.517950046030307
60-61	21.294613478454448	30.946166713081297	28.447918348088457	19.311301460375798
62-63	20.655031671334736	29.527439916701997	30.814922688595658	19.002605723367612
64-65	18.50274674107198	32.484253023011604	30.14472496226224	18.868275273654167
66-67	17.40917661624914	32.21149462802368	27.627674236686246	22.751654519040937
68-69	16.741054244810282	33.553131746012035	27.783503234538166	21.922310774639516
70-71	17.671700141372465	32.661314497861675	29.202886339730277	20.464099021035583
72-73	19.4807533006125	32.56166198677094	28.505531619007414	19.45205309360915
74-75	19.11572143415892	33.255167544997065	26.84707472519286	20.782036295651157
76-77	21.107139752979553	31.286790986928814	27.98390801743298	19.622161242658652
78-79	17.754661122926642	31.730296101703033	29.818965196121038	20.696077579249287
80-81	15.995026243483473	30.990121665463434	31.062954205609135	21.95189788544396
82-83	17.548793012617093	30.54154346094696	30.701523168587997	21.208140357847945
84-85	16.1568684491068	29.89486642341852	30.48366411888768	23.464601008587003
86	16.87064862822589	30.49615172440706	30.171119219382753	22.462080427984297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1915.0
1	2592.5
2	10138.0
3	10388.0
4	4402.0
5	6927.5
6	10789.0
7	12757.0
8	14449.5
9	16183.5
10	15283.5
11	13404.5
12	12103.0
13	11129.5
14	10520.0
15	10224.5
16	9958.0
17	10095.5
18	10375.5
19	10495.0
20	11474.0
21	12505.0
22	13604.5
23	15032.0
24	16906.5
25	19801.5
26	22580.5
27	29799.5
28	38086.0
29	40584.0
30	45004.5
31	53038.5
32	62030.5
33	67344.0
34	72571.0
35	83287.5
36	88712.0
37	92932.5
38	102904.5
39	109784.0
40	118893.5
41	135206.0
42	144908.0
43	146279.0
44	151299.5
45	153052.5
46	149770.5
47	145570.5
48	136765.5
49	122801.0
50	113456.0
51	99631.5
52	80174.0
53	68214.0
54	57649.0
55	50942.0
56	44954.5
57	41436.0
58	35182.5
59	25369.0
60	18469.0
61	13575.5
62	10438.0
63	7447.0
64	6040.0
65	4517.5
66	2553.0
67	1800.5
68	1342.0
69	864.0
70	417.5
71	301.0
72	225.0
73	176.5
74	175.0
75	123.5
76	76.0
77	35.5
78	31.5
79	43.0
80	33.5
81	14.5
82	4.5
83	3.0
84	2.5
85	2.5
86	2.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2818795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.090804329742156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08949448271694	13.765201562358275
2	9.57022544494893	3.462661518321813
3	3.2850044726219703	1.7828511940959566
4	1.7335743390947507	1.2544701663850084
5	1.110308725786461	1.00431889519041
6	0.8322123371697595	0.9033234331521315
7	0.6358916615501848	0.8052654136813312
8	0.5108674498079898	0.7393602458328571
9	0.4136510333267314	0.6734951914238594
>10	3.3410012724128766	13.036792092374133
>50	1.0214387929943451	13.392085186585978
>100	1.4440503157560867	46.22956872477659
>500	0.009110724248371732	1.111283157611006
>1k	0.0029708883418603474	0.9316648287667703
>5k	0.0	0.0
>10k+	1.9805922279068984E-4	0.9076583894438822
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25332	0.8986818835708167	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.095230408738485E-5	0.0	0.0	0.09766584657628526	0.0
2	7.095230408738485E-5	0.0	0.0	0.27909088812772836	0.0
3	7.095230408738485E-5	0.0	0.0	0.3917276708664518	0.0
4	7.095230408738485E-5	0.0	0.0	0.5534634480336456	0.0
5	7.095230408738485E-5	0.0	0.0	0.9724722798216968	0.0
6	7.095230408738485E-5	0.0	0.0	1.3398987865382193	0.0
7	7.095230408738485E-5	0.0	0.0	1.6366922745357502	0.0
8	7.095230408738485E-5	0.0	0.0	2.203530231889868	0.0
9	7.095230408738485E-5	0.0	0.0	2.4295133204081885	0.0
10	7.095230408738485E-5	0.0	0.0	2.810207907989052	0.0
11	7.095230408738485E-5	0.0	0.0	3.4391291314196315	0.0
12	7.095230408738485E-5	0.0	0.0	3.9130550465713188	0.0
13	7.095230408738485E-5	0.0	0.0	4.08976175990095	0.0
14	7.095230408738485E-5	0.0	0.0	4.168767150502253	0.0
15	7.095230408738485E-5	0.0	0.0	4.273776560551584	0.0
16	7.095230408738485E-5	0.0	0.0	4.482376334568495	0.0
17	7.095230408738485E-5	0.0	0.0	4.7799857740630305	0.0
18	7.095230408738485E-5	0.0	0.0	5.103067090724937	0.0
19	7.095230408738485E-5	0.0	0.0	5.340650880961546	0.0
20	7.095230408738485E-5	0.0	0.0	5.556594218451502	0.0
21	7.095230408738485E-5	0.0	0.0	5.807978231833106	0.0
22	7.095230408738485E-5	0.0	0.0	6.0940933980654854	0.0
23	7.095230408738485E-5	0.0	0.0	6.378647613607942	0.0
24	7.095230408738485E-5	0.0	0.0	6.586218579215587	0.0
25	7.095230408738485E-5	0.0	0.0	6.761222437247121	0.0
26	7.095230408738485E-5	0.0	0.0	6.930621063255753	0.0
27	7.095230408738485E-5	0.0	0.0	7.11381991240938	0.0
28	7.095230408738485E-5	0.0	0.0	7.308725891737427	0.0
29	7.095230408738485E-5	0.0	0.0	7.522788993169067	0.0
30	7.095230408738485E-5	0.0	0.0	7.767468013814414	0.0
31	7.095230408738485E-5	0.0	0.0	7.979792783795912	0.0
32	1.0642845613107729E-4	0.0	0.0	8.181439232012261	0.0
33	1.0642845613107729E-4	0.0	0.0	8.3803185403692	0.0
34	1.0642845613107729E-4	0.0	0.0	8.599099970022651	0.0
35	1.0642845613107729E-4	0.0	0.0	8.830617338259788	0.0
36	1.0642845613107729E-4	0.0	0.0	9.046205914229308	0.0
37	1.419046081747697E-4	0.0	0.0	9.267577102981948	0.0
38	1.419046081747697E-4	0.0	0.0	9.503777323288853	0.0
39	1.419046081747697E-4	0.0	0.0	9.782690830656362	0.0
40	1.419046081747697E-4	0.0	0.0	10.039538171452696	0.0
41	1.419046081747697E-4	0.0	0.0	10.295179323079543	0.0
42	1.419046081747697E-4	0.0	0.0	10.556815944401775	0.0
43	1.419046081747697E-4	0.0	0.0	10.799685681292893	0.0
44	1.419046081747697E-4	0.0	0.0	11.035389235471186	0.0
45	1.419046081747697E-4	0.0	0.0	11.27361159644458	0.0
46	1.419046081747697E-4	0.0	0.0	11.521731803838165	0.0
47	1.419046081747697E-4	0.0	0.0	11.783191044400178	0.0
48	1.419046081747697E-4	0.0	0.0	12.038512910658632	0.0
49	1.7738076021846214E-4	0.0	0.0	12.30366167103319	0.0
50	1.7738076021846214E-4	0.0	0.0	12.572748284284597	0.0
51	1.7738076021846214E-4	0.0	0.0	12.837506806986674	0.0
52	1.7738076021846214E-4	0.0	0.0	13.130185061347136	0.0
53	1.7738076021846214E-4	0.0	0.0	13.408318093369685	0.0
54	1.7738076021846214E-4	0.0	0.0	13.684074223205306	0.0
55	1.7738076021846214E-4	0.0	0.0	13.954934644058898	0.0
56	1.7738076021846214E-4	0.0	0.0	14.23349338990597	0.0
57	1.7738076021846214E-4	0.0	0.0	14.522269267541628	0.0
58	2.48333064305847E-4	0.0	0.0	14.801856821797967	0.0
59	2.48333064305847E-4	0.0	0.0	15.081337947598175	0.0
60	2.48333064305847E-4	0.0	0.0	15.378592625572274	0.0
61	2.48333064305847E-4	0.0	0.0	15.69386918878457	0.0
62	2.48333064305847E-4	0.0	0.0	16.031424775480303	0.0
63	2.838092163495394E-4	0.0	0.0	16.371818454339532	0.0
64	3.547615204369243E-4	0.0	0.0	16.68769811213657	0.0
65	3.547615204369243E-4	0.0	0.0	17.003542293781564	0.0
66	3.547615204369243E-4	0.0	0.0	17.29664626196655	0.0
67	3.902376724806167E-4	0.0	0.0	17.589963087063797	0.0
68	3.902376724806167E-4	0.0	0.0	17.92443224853173	0.0
69	4.2571382452430916E-4	0.0	0.0	18.280258053529966	0.0
70	4.611899765680016E-4	0.0	0.0	18.602026752566257	0.0
71	4.611899765680016E-4	0.0	0.0	18.923582594690284	0.0
72	4.611899765680016E-4	0.0	0.0	19.24595438831132	0.0
73	4.611899765680016E-4	0.0	0.0	19.57456998469204	0.0
74	4.611899765680016E-4	0.0	0.0	19.92273294084884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATA	715	0.0	72.72727	6
ACGGGAT	2075	0.0	71.710846	5
CGTTTTT	10715	0.0	71.37657	1
ACGGGTA	220	0.0	70.90909	5
TACGGGA	1835	0.0	70.84469	4
CGAGGGA	2330	0.0	68.8412	4
AGTACGG	425	0.0	68.70588	2
GCGCGAG	190	0.0	67.368416	1
GGCGCGA	120	0.0	66.66667	8
CAGCGCG	145	0.0	66.206894	1
TATAGCG	115	0.0	66.08696	1
ACGGGTC	170	0.0	65.882355	5
TACGCGG	170	0.0	65.882355	2
TAGGGAC	3285	0.0	65.87519	5
TTAGGGA	4560	0.0	65.61403	4
GGGCGAT	1725	0.0	65.159424	7
TAGCGAG	320	0.0	65.0	1
AGGGCGA	985	0.0	64.97462	6
TTGCGGG	2305	0.0	64.90239	3
CGCGAGG	450	0.0	64.888885	2
>>END_MODULE