FastQCFastQC Report
Thu 26 May 2016
SRR1547802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547802_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2725773
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196510.7209331077826364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32830.12044289821639587No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC31430.11530674050993975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92450.073.38021
GTCGACG550.072.727271
CGGGATA7650.071.633996
GACGGGT5600.070.04
ACGGGTC3350.069.253735
TACGGGA13400.068.955224
GCGCGAC1600.067.59
CGTAAGG4050.066.1728442
CGCGGGA9000.064.8888854
AGTACGG4250.064.02
CCGATCC21700.063.59446780
CGAGGGT7050.063.54614
GGCGCGA1700.063.529418
TACGAAG2650.063.396231
TAACGGG8150.063.3128853
CTACGGG6450.063.2558173
AGGGCGA11100.063.063066
GGGCGAT19650.062.9007647
TGGGCGA6200.062.5806476
GCGGGCT4950.062.2222185
Produced by FastQC (version 0.11.5)