##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547802_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2725773
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.703577664024113	31.0	31.0	33.0	30.0	34.0
2	32.14962214388359	33.0	31.0	34.0	30.0	34.0
3	32.28987300116334	34.0	31.0	34.0	30.0	34.0
4	35.890526467170965	37.0	35.0	37.0	35.0	37.0
5	35.83577062359925	37.0	35.0	37.0	35.0	37.0
6	35.894644198177915	37.0	35.0	37.0	35.0	37.0
7	36.21104288581624	37.0	35.0	37.0	35.0	37.0
8	36.18280649195659	37.0	35.0	37.0	35.0	37.0
9	38.04640041558853	39.0	39.0	39.0	35.0	39.0
10-11	37.3984647657747	39.0	37.0	39.0	34.5	39.0
12-13	36.763774899817406	39.0	35.0	39.0	33.0	39.0
14-15	37.86604442116053	40.0	36.0	41.0	33.0	41.0
16-17	37.99508249586448	40.0	36.0	41.0	33.0	41.0
18-19	37.88060286017948	40.0	36.0	41.0	33.5	41.0
20-21	37.691028746707815	40.0	35.0	41.0	33.0	41.0
22-23	37.485455868848945	39.0	35.0	41.0	33.0	41.0
24-25	37.3856795852039	39.0	35.0	41.0	33.0	41.0
26-27	37.32887716622037	39.0	35.0	41.0	33.0	41.0
28-29	37.31517554836738	39.0	35.0	41.0	33.0	41.0
30-31	37.14819300800177	39.0	35.0	41.0	32.5	41.0
32-33	36.839408490729056	39.0	35.0	41.0	31.5	41.0
34-35	36.56228416672995	39.0	35.0	41.0	31.0	41.0
36-37	36.36228640462724	39.0	35.0	41.0	30.0	41.0
38-39	36.238835735771104	39.0	35.0	41.0	30.0	41.0
40-41	36.09214780541153	38.0	35.0	41.0	30.0	41.0
42-43	36.03298660600131	38.0	35.0	41.0	30.0	41.0
44-45	35.90399530701933	38.0	35.0	41.0	29.5	41.0
46-47	35.811464307556065	38.0	35.0	40.5	29.0	41.0
48-49	35.69205964693318	38.0	35.0	40.0	29.0	41.0
50-51	35.51971936034292	38.0	35.0	40.0	28.0	41.0
52-53	35.33754296487639	37.0	35.0	40.0	28.0	41.0
54-55	35.16106146770109	37.0	34.0	40.0	27.5	41.0
56-57	34.851379590303374	36.5	34.0	40.0	26.5	41.0
58-59	34.56183970565414	36.0	34.0	40.0	26.0	41.0
60-61	34.237291586643494	35.5	34.0	39.0	26.0	41.0
62-63	33.82185952388552	35.0	33.0	39.0	24.5	40.5
64-65	33.475022865073505	35.0	33.0	38.5	24.0	40.0
66-67	33.160556840206425	35.0	33.0	38.0	23.0	40.0
68-69	32.7224271793726	35.0	32.5	37.0	21.0	40.0
70-71	32.42135056734365	35.0	32.0	36.5	20.5	39.0
72-73	32.12635002988143	35.0	32.0	36.0	20.0	39.0
74-75	31.807649793287993	35.0	32.0	36.0	19.5	38.5
76-77	29.733415988785566	33.0	28.5	34.5	16.0	36.0
78-79	30.830912552145758	34.0	30.5	35.0	17.5	37.0
80-81	31.0440445701091	34.5	31.0	35.0	18.0	36.5
82-83	30.853329129021382	34.5	31.0	35.0	16.0	36.0
84-85	30.64404684469323	34.0	31.0	35.0	15.0	36.0
86	30.23709494517702	34.0	30.0	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	7.0
10	8.0
11	19.0
12	40.0
13	112.0
14	410.0
15	1276.0
16	3267.0
17	7061.0
18	14167.0
19	21198.0
20	23946.0
21	23120.0
22	20954.0
23	20513.0
24	22354.0
25	25768.0
26	30686.0
27	37399.0
28	45535.0
29	55649.0
30	68419.0
31	82028.0
32	98593.0
33	125447.0
34	274024.0
35	276772.0
36	243582.0
37	389379.0
38	550416.0
39	262690.0
40	928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.962903367228304	25.030550966643222	32.45714151545268	13.54940415067579
2	29.910781271954782	26.152214436051718	31.264855877580416	12.672148414413087
3	28.024123799010408	24.949913290651864	34.05349601746	12.972466892877726
4	25.99765277592815	26.790785586327253	32.430029940130744	14.78153169761385
5	23.760892781607275	31.323261328070974	30.61271059622353	14.303135294098224
6	21.582501550936193	41.13467996051028	26.652989812431187	10.629828676122333
7	88.4845509879216	3.516983989495824	6.7226801351396475	1.2757848874429383
8	90.06373604845305	2.5520833906565223	5.87077500584238	1.513405555048054
9	85.33073003511298	5.51751741616048	7.222171472092503	1.929581076634041
10-11	36.3104337741991	29.152904515526423	22.519171625810365	12.017490084464114
12-13	27.957298718565337	27.72206269560965	27.630290563447506	16.690348022377506
14-15	16.60385879528486	32.78303072192732	33.90381370715757	16.709296775630254
16-17	20.839648055799216	26.807551472554756	32.65422322401756	19.69857724762847
18-19	22.726892518195758	28.884228437217622	30.25303280940856	18.135846235178057
20-21	24.940961701506325	29.295487922141717	29.18656469192409	16.576985684427868
22-23	20.642272852508263	26.835506845214184	31.134709309982895	21.38751099229466
24-25	18.462157340321443	29.259076232687022	34.104930968206084	18.173835458785454
26-27	17.954026252369513	35.316257076433	29.08593268771831	17.64378398347918
28-29	16.09857093749186	30.473759186843512	35.34877996076709	18.078889914897537
30-31	21.291776681330397	31.097197015305383	29.81565229386306	17.79537400950116
32-33	23.188302914439316	30.96939840551653	28.294047229905058	17.548251450139098
34-35	18.299891443638188	30.873755811654163	29.827740607893617	20.998612136814035
36-37	21.655508364049393	30.989190956106764	29.443482637769176	17.91181804207467
38-39	19.25578542307081	32.54491478197194	28.283903318434806	19.91539647652244
40-41	20.227289653246988	28.978623678494138	28.940909606192445	21.853177062066433
42-43	20.56418491194975	28.853374804138127	28.060333710840922	22.5221065730712
44-45	19.145082881076302	33.32212550348103	27.973092403512688	19.559699211929974
46-47	20.73648465958097	32.12512560657105	27.256690120563963	19.881699613284013
48-49	20.649738624603003	29.661916087656603	30.075064944879852	19.61328034286054
50-51	18.705978084015065	32.609520308551005	28.366375336464188	20.31812627096974
52-53	21.062667360781695	29.055042367798052	29.280703125315277	20.601587146104976
54-55	21.01956032288822	29.438676661629565	30.138514835974966	19.403248179507244
56-57	21.459490573866567	27.53174970916507	29.46388052123196	21.544879195736403
58-59	22.039692960492307	29.494495689846513	28.401319552288466	20.064491797372707
60-61	22.41147740475821	31.314401456027337	26.979521038619136	19.294600100595318
62-63	21.98501122433893	28.898004345923155	29.492643004388114	19.6243414253498
64-65	19.164288442214374	33.1015642168295	28.8285744997841	18.905572841172027
66-67	18.076596987350012	31.909186861855332	25.997340937781686	24.01687521301297
68-69	17.88962250341463	34.16407015551185	25.493557240459864	22.45275010061366
70-71	18.91158948305673	32.112523676769854	28.04542051007182	20.93046633010159
72-73	21.14092039212363	31.74050076803901	27.103816055115377	20.014762784721984
74-75	20.552665243950983	33.427031524635396	24.837413093460096	21.182890137953528
76-77	23.952581524580367	31.047321255291614	25.7128161442644	19.287281075863618
78-79	18.456048981334835	31.84282036691977	29.0759355236111	20.625195128134294
80-81	16.845129803545635	30.748451907037012	29.75532078423258	22.651097505184765
82-83	18.66421011580935	29.749689354175864	30.651268465862714	20.93483206415208
84-85	16.769371477375408	29.37621731523498	29.1217023574597	24.73270884992991
86	17.98172481714361	29.5939170283072	30.055547545595324	22.36881060895386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2412.0
1	2821.5
2	7888.5
3	7980.0
4	3680.0
5	5471.0
6	8940.0
7	10884.0
8	11652.0
9	12624.0
10	12302.0
11	11260.5
12	10372.5
13	9356.0
14	8712.0
15	8799.0
16	8844.0
17	8864.5
18	8934.0
19	8937.5
20	9201.0
21	9468.0
22	9969.0
23	11215.5
24	13534.0
25	17586.5
26	20862.0
27	23953.0
28	29707.5
29	33167.0
30	36601.5
31	44433.0
32	52140.0
33	56545.5
34	59649.5
35	68257.0
36	74856.0
37	79588.0
38	86583.0
39	97899.5
40	110442.0
41	124295.5
42	139166.5
43	143673.0
44	146855.0
45	152805.0
46	156974.0
47	160270.0
48	157041.0
49	137898.0
50	123879.0
51	108860.0
52	85948.5
53	73425.5
54	62928.0
55	55395.5
56	49135.0
57	44540.0
58	39192.5
59	29280.5
60	20374.0
61	14645.0
62	11417.0
63	7734.5
64	5893.0
65	4697.5
66	2874.0
67	2022.0
68	1718.5
69	1165.0
70	517.5
71	344.0
72	314.0
73	206.0
74	143.0
75	152.5
76	94.0
77	39.5
78	30.0
79	22.0
80	19.5
81	9.5
82	3.5
83	4.5
84	4.0
85	3.5
86	3.0
87	2.5
88	1.0
89	0.5
90	0.5
91	1.5
92	1.5
93	0.0
94	1.0
95	1.0
96	0.5
97	0.5
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2725773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.486350091065248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62093154969317	13.747931335636117
2	8.687602660892544	3.0382892316087404
3	2.7754296886619465	1.4559640556723705
4	1.3651626830361354	0.9548685042731122
5	0.8742436979943008	0.764366568401793
6	0.6228287116691135	0.6534600539407952
7	0.5382989937041146	0.6589019260304794
8	0.4237713761598918	0.5928171713683495
9	0.34965227221532763	0.5502727837884298
>10	3.175079678607065	12.043642008967549
>50	1.0020473598087656	12.748273255205769
>100	1.5532963079819213	50.190751332152615
>500	0.008052558979549972	0.9101134487377722
>1k	0.0033905511492841986	0.9621760437248073
>5k	0.0	0.0
>10k+	2.119094468302624E-4	0.728172280491265
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19651	0.7209331077826364	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3283	0.12044289821639587	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	3143	0.11530674050993975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.337368152080162E-5	0.0	0.0	0.0497473560711035	0.0
2	7.337368152080162E-5	0.0	0.0	0.1760234619684031	0.0
3	7.337368152080162E-5	0.0	0.0	0.2783063740084005	0.0
4	7.337368152080162E-5	0.0	0.0	0.43103369209394915	0.0
5	7.337368152080162E-5	0.0	0.0	0.7509796303654046	0.0
6	7.337368152080162E-5	0.0	0.0	1.02605756238689	0.0
7	7.337368152080162E-5	0.0	0.0	1.2313938101228532	0.0
8	7.337368152080162E-5	0.0	0.0	1.6157251539288122	0.0
9	7.337368152080162E-5	0.0	0.0	1.7382958889093112	0.0
10	7.337368152080162E-5	0.0	0.0	1.9424581577409417	0.0
11	7.337368152080162E-5	0.0	0.0	2.3623757370844896	0.0
12	7.337368152080162E-5	0.0	0.0	2.6340784797560177	0.0
13	7.337368152080162E-5	0.0	0.0	2.7806057217530586	0.0
14	7.337368152080162E-5	0.0	0.0	2.817769491443345	0.0
15	7.337368152080162E-5	0.0	0.0	2.8938213123396554	0.0
16	7.337368152080162E-5	0.0	0.0	3.0300395520830237	0.0
17	7.337368152080162E-5	0.0	0.0	3.2093281428791025	0.0
18	7.337368152080162E-5	0.0	0.0	3.4167188536976485	0.0
19	7.337368152080162E-5	0.0	0.0	3.559173856370285	0.0
20	7.337368152080162E-5	0.0	0.0	3.696309267132663	0.0
21	7.337368152080162E-5	0.0	0.0	3.869801337088598	0.0
22	7.337368152080162E-5	0.0	0.0	4.075137584824562	0.0
23	7.337368152080162E-5	0.0	0.0	4.269357719810124	0.0
24	7.337368152080162E-5	0.0	0.0	4.417572556482143	0.0
25	7.337368152080162E-5	0.0	0.0	4.555294956696687	0.0
26	7.337368152080162E-5	0.0	0.0	4.68182787047931	0.0
27	7.337368152080162E-5	0.0	0.0	4.8155880918917315	0.0
28	7.337368152080162E-5	0.0	0.0	4.958520023494253	0.0
29	7.337368152080162E-5	0.0	0.0	5.127169430469816	0.0
30	7.337368152080162E-5	0.0	0.0	5.3027893371898545	0.0
31	7.337368152080162E-5	0.0	0.0	5.473456520407239	0.0
32	7.337368152080162E-5	0.0	0.0	5.6196168939966755	0.0
33	7.337368152080162E-5	0.0	0.0	5.778911156578336	0.0
34	7.337368152080162E-5	0.0	0.0	5.945616160993597	0.0
35	7.337368152080162E-5	0.0	0.0	6.145265948411698	0.0
36	7.337368152080162E-5	0.0	0.0	6.3174372921002595	0.0
37	1.1006052228120243E-4	0.0	0.0	6.500211132768576	0.0
38	1.1006052228120243E-4	0.0	0.0	6.6906525231558165	0.0
39	1.1006052228120243E-4	0.0	0.0	6.926328788200632	0.0
40	1.1006052228120243E-4	0.0	0.0	7.132618893796366	0.0
41	1.1006052228120243E-4	0.0	0.0	7.346136307021898	0.0
42	1.1006052228120243E-4	0.0	0.0	7.563395778004992	0.0
43	1.1006052228120243E-4	0.0	0.0	7.768511904696393	0.0
44	1.4674736304160323E-4	0.0	0.0	7.975645807629616	0.0
45	1.4674736304160323E-4	0.0	0.0	8.180578500117214	0.0
46	1.8343420380200406E-4	0.0	0.0	8.409651133825157	0.0
47	1.8343420380200406E-4	0.0	0.0	8.64246582529066	0.0
48	2.2012104456240486E-4	0.0	0.0	8.867319472311157	0.0
49	2.2012104456240486E-4	0.0	0.0	9.102995737355972	0.0
50	2.568078853228057E-4	0.0	0.0	9.350705286170198	0.0
51	2.568078853228057E-4	0.0	0.0	9.602817255875673	0.0
52	2.9349472608320646E-4	0.0	0.0	9.875473856406972	0.0
53	3.301815668436073E-4	0.0	0.0	10.133749215360194	0.0
54	3.301815668436073E-4	0.0	0.0	10.399361942465495	0.0
55	3.301815668436073E-4	0.0	0.0	10.659875198705102	0.0
56	3.301815668436073E-4	0.0	0.0	10.926368409988653	0.0
57	3.668684076040081E-4	0.0	0.0	11.192971681794486	0.0
58	3.668684076040081E-4	0.0	0.0	11.453191443308008	0.0
59	3.668684076040081E-4	0.0	0.0	11.725848043839306	0.0
60	3.668684076040081E-4	0.0	0.0	12.011418412318267	0.0
61	4.0355524836440895E-4	0.0	0.0	12.30179475693684	0.0
62	4.0355524836440895E-4	0.0	0.0	12.625592813488137	0.0
63	4.0355524836440895E-4	0.0	0.0	12.955260764561098	0.0
64	4.0355524836440895E-4	0.0	0.0	13.271427958234232	0.0
65	4.402420891248097E-4	0.0	0.0	13.567454076329907	0.0
66	4.402420891248097E-4	0.0	0.0	13.870193886284735	0.0
67	4.402420891248097E-4	0.0	0.0	14.17645563295256	0.0
68	4.402420891248097E-4	0.0	0.0	14.506490452433127	0.0
69	4.402420891248097E-4	0.0	0.0	14.899736698543862	0.0
70	4.402420891248097E-4	0.0	0.0	15.210804421351302	0.0
71	4.402420891248097E-4	0.0	0.0	15.538161101456357	0.0
72	4.7692892988521055E-4	0.0	0.0	15.853998113562648	0.0
73	5.136157706456114E-4	0.0	0.0	16.184032933043213	3.668684076040081E-5
74	5.136157706456114E-4	0.0	0.0	16.544774638240234	3.668684076040081E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9245	0.0	73.3802	1
GTCGACG	55	0.0	72.72727	1
CGGGATA	765	0.0	71.63399	6
GACGGGT	560	0.0	70.0	4
ACGGGTC	335	0.0	69.25373	5
TACGGGA	1340	0.0	68.95522	4
GCGCGAC	160	0.0	67.5	9
CGTAAGG	405	0.0	66.172844	2
CGCGGGA	900	0.0	64.888885	4
AGTACGG	425	0.0	64.0	2
CCGATCC	2170	0.0	63.594467	80
CGAGGGT	705	0.0	63.5461	4
GGCGCGA	170	0.0	63.52941	8
TACGAAG	265	0.0	63.39623	1
TAACGGG	815	0.0	63.312885	3
CTACGGG	645	0.0	63.255817	3
AGGGCGA	1110	0.0	63.06306	6
GGGCGAT	1965	0.0	62.900764	7
TGGGCGA	620	0.0	62.580647	6
GCGGGCT	495	0.0	62.222218	5
>>END_MODULE