FastQCFastQC Report
Thu 26 May 2016
SRR1547801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547801_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2377693
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124910.5253411605282936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26070.10964409618903703No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT23900.10051760256685788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG2000.076.01
TAGGGAC26750.071.32715
CGTTAGG3200.071.252
CGTTTTT61450.070.3010561
GTTAGCG2000.070.01
TTAGGGA33850.068.301334
TAGTAGG14950.065.5518342
TCGTTAG800.065.01
GGGCGAT13750.064.872737
ACTAGGG13950.063.9426543
CGGGATA5650.063.008856
AGTAGGG35250.062.865253
CTACGGG3450.062.60873
AGGGTAA15750.062.476196
AGGGATT41900.062.147976
TAGGGAG29000.061.7931025
TAGGGTA16300.061.5950975
TAATACG850.061.1764681
TAGCACG1250.060.81
CTAGGGC5150.060.5825234
Produced by FastQC (version 0.11.5)