FastQCFastQC Report
Thu 26 May 2016
SRR1547800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547800_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2200477
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124140.5641504092067311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG2250.069.3333362
CGTTTTT61450.069.259561
ACGGGAT12300.067.317085
TAGGGAC25850.066.382985
CGAGGGA14200.065.6338044
CGTTAGG4300.065.116282
TAGCACG800.065.01
TAAGGGA33550.063.6661724
TAACGGG7150.063.2167853
GGCGATT3800.063.1578948
ATAGGGA34850.062.8981324
TACGGGA10000.062.44
AGCCGTA1800.062.2222280
GTACGGG7950.061.886793
CACGGGA5900.061.6949164
CGGGATC7400.061.621626
CCGATCC20600.060.58252380
GCGCGAC402.977249E-760.09
TAGGGAG30300.059.9339945
TAGCGGG12900.059.8449633
Produced by FastQC (version 0.11.5)