##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547800_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2200477
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74010816745642	31.0	31.0	33.0	30.0	34.0
2	32.17888303308783	33.0	31.0	34.0	30.0	34.0
3	32.330590594675606	34.0	31.0	34.0	30.0	34.0
4	35.93899913518751	37.0	35.0	37.0	35.0	37.0
5	35.88956939790782	37.0	35.0	37.0	35.0	37.0
6	35.9512273929698	37.0	35.0	37.0	35.0	37.0
7	36.20666201010054	37.0	35.0	37.0	35.0	37.0
8	36.157217730519335	37.0	35.0	37.0	35.0	37.0
9	38.02905506397022	39.0	39.0	39.0	35.0	39.0
10-11	37.495670256948834	39.0	37.0	39.0	35.0	39.0
12-13	36.70829665567965	39.0	35.0	39.0	33.0	39.0
14-15	37.72890127913175	40.0	35.0	41.0	33.0	41.0
16-17	37.91431766839644	40.0	35.0	41.0	33.0	41.0
18-19	37.8053358430922	40.0	36.0	41.0	33.5	41.0
20-21	37.63517182865351	40.0	35.0	41.0	33.0	41.0
22-23	37.438633532638605	39.0	35.0	41.0	33.0	41.0
24-25	37.363382575687	39.0	35.0	41.0	33.0	41.0
26-27	37.33914828466737	39.0	35.0	41.0	33.0	41.0
28-29	37.33700534020578	39.0	35.0	41.0	33.0	41.0
30-31	37.16664068745095	39.0	35.0	41.0	32.5	41.0
32-33	36.8768337501369	39.0	35.0	41.0	31.5	41.0
34-35	36.598540452820004	39.0	35.0	41.0	31.0	41.0
36-37	36.42702014154204	39.0	35.0	41.0	30.5	41.0
38-39	36.336924221430166	39.0	35.0	41.0	30.0	41.0
40-41	36.17354328175209	38.5	35.0	41.0	30.0	41.0
42-43	36.128928182389544	38.0	35.0	41.0	30.0	41.0
44-45	36.035733615938724	38.0	35.0	41.0	30.0	41.0
46-47	35.95855398624934	38.0	35.0	41.0	30.0	41.0
48-49	35.851745326127016	38.0	35.0	40.0	29.5	41.0
50-51	35.662317533880156	38.0	35.0	40.0	29.0	41.0
52-53	35.473661619730635	37.0	35.0	40.0	29.0	41.0
54-55	35.26940499718924	37.0	34.0	40.0	28.5	41.0
56-57	34.968269606998845	36.5	34.0	40.0	27.5	41.0
58-59	34.666076718820506	36.0	34.0	39.5	27.0	41.0
60-61	34.35938366999518	35.5	34.0	39.0	26.0	41.0
62-63	33.978644630232445	35.0	33.5	39.0	26.0	40.5
64-65	33.71453325801633	35.0	33.0	38.5	26.0	40.0
66-67	33.443410451461205	35.0	33.0	37.5	25.0	40.0
68-69	33.03277448480489	35.0	33.0	37.0	24.0	39.5
70-71	32.817078978785055	35.0	33.0	36.5	24.0	39.0
72-73	32.57011116226164	35.0	33.0	36.0	24.0	39.0
74-75	32.28665262122712	35.0	32.5	36.0	23.5	38.5
76-77	30.240867548263402	33.0	29.5	34.5	19.5	36.0
78-79	31.372701464273426	34.0	31.0	35.0	20.0	37.0
80-81	31.62573591998462	35.0	32.0	35.0	20.0	36.5
82-83	31.498951590950504	35.0	32.0	35.0	20.0	36.0
84-85	31.302211520502144	34.0	32.0	35.0	20.0	36.0
86	30.88656504930522	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	25.0
11	48.0
12	61.0
13	133.0
14	344.0
15	919.0
16	2286.0
17	4937.0
18	9336.0
19	14213.0
20	17266.0
21	17884.0
22	16489.0
23	15728.0
24	16639.0
25	18787.0
26	22079.0
27	26985.0
28	32740.0
29	39950.0
30	49833.0
31	61478.0
32	76718.0
33	104193.0
34	246927.0
35	221296.0
36	196135.0
37	331390.0
38	447650.0
39	207163.0
40	828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.22852272484557	23.970302802528725	28.86046979813922	13.940704674486486
2	31.021910249459552	25.1710879050315	31.027181833756956	12.779820011751998
3	29.049065270848097	25.029118686539327	32.615837384348936	13.30597865826364
4	27.340481177490155	27.010552711980175	31.423686773367777	14.225279337161897
5	23.640147113557653	31.182466347069294	30.431083805920263	14.746302733452794
6	22.297392792562704	40.91830998460788	26.587099069883486	10.19719815294593
7	88.37029425892659	3.5566833918282263	6.392841188524125	1.680181160721062
8	88.9198114772388	3.182219127943623	6.25464387948613	1.6433255153314488
9	84.47777459159991	5.916898926914483	7.447067158620609	2.158259322864997
10-11	39.24880832655829	28.66726168917012	19.817930385093778	12.265999599177814
12-13	30.51829217028853	25.18578926296435	27.397559710917225	16.898358855829894
14-15	17.28241194977271	31.140498173805042	34.40558569801002	17.171504178412224
16-17	23.145027191831588	26.179073900795146	31.219662827650552	19.456236079722714
18-19	24.547245892595104	28.46451019483503	29.666272358220514	17.321971554349354
20-21	26.882012400038718	28.27327892997745	28.99589498095186	15.848813689031967
22-23	22.761178599003763	23.88604834315469	32.040053133934144	21.31271992390741
24-25	21.009422048037766	26.774717481709647	33.187122610234056	19.028737860018534
26-27	18.57794923555211	33.0471075135073	29.253498218795286	19.121445032145303
28-29	17.370142928101497	29.000212226712662	34.58713724342495	19.042507601760892
30-31	22.745591069572644	28.33492465497254	30.507158220694876	18.412326054759944
32-33	25.910359435704166	27.71739945475458	28.384732037644568	17.987509071896685
34-35	20.068785086142686	28.115608570323612	30.659238883205774	21.15636746032792
36-37	23.3086053614739	29.277402126902487	28.948473444621325	18.46551906700229
38-39	20.435660086426715	31.51805267676054	27.564591677168178	20.48169555964457
40-41	21.035507301371474	26.992829282014764	28.653378335697216	23.318285080916546
42-43	20.570108208356643	26.823547803498972	28.650197207241884	23.956146780902504
44-45	19.692889314453186	30.143623405288945	28.505342250793802	21.658145029464066
46-47	21.106196520118136	29.439389732317128	28.326630998642564	21.127782748922165
48-49	21.14264316327778	27.412533737003386	30.443240261088846	21.001582838629986
50-51	19.55051109373104	30.544377423622244	29.080490275517533	20.824621207129184
52-53	22.0960500836864	27.01048454494185	29.68697241552627	21.206492955845484
54-55	21.833175261545566	27.67968036021281	29.793017604819315	20.694126773422308
56-57	21.878756287841227	26.585599395040255	29.457181329320868	22.078462987797646
58-59	23.290586541009063	27.802585530319107	28.28911640521578	20.61771152345605
60-61	24.21906704773556	29.06690231254405	27.164701107987042	19.54932953173335
62-63	22.943661760609178	26.493778394411756	29.82551055975591	20.737049285223158
64-65	20.551339550470193	29.29721601270997	29.99372408800455	20.157720348815282
66-67	19.696456722792377	29.6066943667214	26.569284750533633	24.12756415995259
68-69	18.71719177251114	31.359677924377305	26.759675288585154	23.163455014526395
70-71	19.61595145052641	30.036669322151514	29.120731550477462	21.226647676844614
72-73	22.117340921991005	30.184296404824956	27.081310097765165	20.617052575418874
74-75	20.847184496815917	31.6222119113265	25.927128527132982	21.603475064724602
76-77	24.03251658617654	28.24385349176565	26.954723907589127	20.76890601446868
78-79	19.5980007970999	29.640346161309573	28.873739648267172	21.88791339332336
80-81	18.22922938980957	28.664807675790293	29.89036013555243	23.215602798847705
82-83	19.254461646270332	27.793246646068106	30.85433294690197	22.0979587607596
84-85	18.168810671504406	27.280948630683255	29.075195968874024	25.475044728938318
86	18.970159651748233	27.69058708634537	29.460657848275623	23.87859541363077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1351.0
1	1764.5
2	5154.0
3	5318.5
4	2888.5
5	3741.5
6	4537.5
7	4862.0
8	4911.5
9	5006.0
10	5024.0
11	4884.5
12	4751.5
13	4956.5
14	5182.0
15	5063.0
16	5111.0
17	5485.5
18	6395.0
19	6712.0
20	6987.0
21	7647.0
22	7762.5
23	8871.0
24	9604.0
25	12135.5
26	15902.0
27	19771.0
28	24082.5
29	25500.0
30	27978.5
31	33058.0
32	38717.5
33	42392.0
34	45316.0
35	49184.5
36	50745.0
37	55897.5
38	62077.0
39	69406.0
40	82980.5
41	91429.0
42	97802.0
43	102999.0
44	106856.5
45	113963.5
46	117067.5
47	121418.0
48	125899.0
49	115741.0
50	105598.0
51	94281.0
52	77288.0
53	68379.5
54	63877.5
55	62013.5
56	58027.5
57	54636.0
58	48198.0
59	35916.0
60	27134.0
61	21089.5
62	16202.5
63	12643.0
64	10864.0
65	9039.5
66	6222.5
67	4744.5
68	3450.5
69	2578.0
70	2088.5
71	1663.0
72	1166.0
73	478.5
74	277.5
75	220.5
76	129.0
77	73.0
78	95.0
79	128.0
80	80.5
81	31.0
82	24.0
83	16.5
84	12.0
85	7.5
86	5.0
87	3.5
88	2.5
89	3.0
90	1.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2200477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.921395891626597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60115119539334	12.832828375027097
2	7.922901703355009	2.522873092591156
3	2.3601674289858297	1.127314800222177
4	1.1188495965052458	0.7125458947658679
5	0.6793427123178066	0.5408042134451597
6	0.5088995296418404	0.4861434528294183
7	0.39445146072575815	0.4396152506371638
8	0.29689969564113283	0.3781646075524736
9	0.262785582172964	0.37655219595485995
>10	2.719843953144392	10.392163524461333
>50	1.2449009178442028	14.539092727939066
>100	1.8791537516493384	53.56345662761706
>500	0.006333902640744782	0.630324410206063
>1k	0.004030665316837588	0.8890824129413715
>5k	0.0	0.0
>10k+	2.879046654883992E-4	0.5690384138097642
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12414	0.5641504092067311	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.088938443801048E-5	0.0	0.0	0.0373100923118033	0.0
2	9.088938443801048E-5	0.0	0.0	0.1056589094091872	0.0
3	9.088938443801048E-5	0.0	0.0	0.1525578317792006	0.0
4	9.088938443801048E-5	0.0	0.0	0.22236087902759266	0.0
5	9.088938443801048E-5	0.0	0.0	0.3924149173111103	0.0
6	9.088938443801048E-5	0.0	0.0	0.5230229627485313	0.0
7	9.088938443801048E-5	0.0	0.0	0.6158210242597401	0.0
8	9.088938443801048E-5	0.0	0.0	0.823457823008375	0.0
9	9.088938443801048E-5	0.0	0.0	0.8903069652625317	0.0
10	9.088938443801048E-5	0.0	0.0	1.0199606721633536	0.0
11	9.088938443801048E-5	0.0	0.0	1.2709062625967007	0.0
12	9.088938443801048E-5	0.0	0.0	1.4515034694750275	0.0
13	9.088938443801048E-5	0.0	0.0	1.5185343904980602	0.0
14	9.088938443801048E-5	0.0	0.0	1.5476644382104425	0.0
15	9.088938443801048E-5	0.0	0.0	1.5964265929614352	0.0
16	9.088938443801048E-5	0.0	0.0	1.6885429840893589	0.0
17	9.088938443801048E-5	0.0	0.0	1.7956106789573352	0.0
18	9.088938443801048E-5	0.0	0.0	1.916175447414356	0.0
19	9.088938443801048E-5	0.0	0.0	2.003020254244875	0.0
20	9.088938443801048E-5	0.0	0.0	2.100771787207955	0.0
21	9.088938443801048E-5	0.0	0.0	2.2307436069543103	0.0
22	9.088938443801048E-5	0.0	0.0	2.367304907072421	0.0
23	9.088938443801048E-5	0.0	0.0	2.4990490698153174	0.0
24	9.088938443801048E-5	0.0	0.0	2.5964824899328645	0.0
25	9.088938443801048E-5	0.0	0.0	2.6933251290515647	0.0
26	9.088938443801048E-5	0.0	0.0	2.7888498720959136	0.0
27	9.088938443801048E-5	0.0	0.0	2.902098045105675	0.0
28	9.088938443801048E-5	0.0	0.0	3.010165523202469	0.0
29	9.088938443801048E-5	0.0	0.0	3.138410444644502	0.0
30	9.088938443801048E-5	0.0	0.0	3.282561008363187	0.0
31	9.088938443801048E-5	0.0	0.0	3.4106695957285624	0.0
32	9.088938443801048E-5	0.0	0.0	3.526917118424778	0.0
33	9.088938443801048E-5	0.0	0.0	3.6464821036529806	0.0
34	9.088938443801048E-5	0.0	0.0	3.774090799403947	0.0
35	9.088938443801048E-5	0.0	0.0	3.9303750959451063	0.0
36	1.3633407665701572E-4	0.0	0.0	4.061346698920279	0.0
37	1.3633407665701572E-4	0.0	0.0	4.2049519263323365	0.0
38	1.3633407665701572E-4	0.0	0.0	4.379686767914412	0.0
39	1.3633407665701572E-4	0.0	0.0	4.6834845353984615	0.0
40	1.3633407665701572E-4	0.0	0.0	4.8618549523580565	0.0
41	1.3633407665701572E-4	0.0	0.0	5.036498904555694	0.0
42	1.3633407665701572E-4	0.0	0.0	5.211779082444397	0.0
43	1.3633407665701572E-4	0.0	0.0	5.367699821447804	0.0
44	1.3633407665701572E-4	0.0	0.0	5.5258019056777234	0.0
45	1.3633407665701572E-4	0.0	0.0	5.678041624611391	0.0
46	1.3633407665701572E-4	0.0	0.0	5.848686443893755	0.0
47	1.3633407665701572E-4	0.0	0.0	6.027738531236636	0.0
48	1.3633407665701572E-4	0.0	0.0	6.216561227406603	0.0
49	1.3633407665701572E-4	0.0	0.0	6.397203878977149	0.0
50	1.3633407665701572E-4	0.0	0.0	6.584072453381698	0.0
51	1.3633407665701572E-4	0.0	0.0	6.774440269087112	0.0
52	1.3633407665701572E-4	0.0	0.0	7.012206898776947	0.0
53	1.3633407665701572E-4	0.0	0.0	7.213754108768235	0.0
54	1.3633407665701572E-4	0.0	0.0	7.419891232673643	0.0
55	1.3633407665701572E-4	0.0	0.0	7.619620654976171	0.0
56	1.3633407665701572E-4	0.0	0.0	7.826075891727112	4.544469221900524E-5
57	1.3633407665701572E-4	0.0	0.0	8.033758135167966	4.544469221900524E-5
58	1.3633407665701572E-4	0.0	0.0	8.235714347389225	4.544469221900524E-5
59	1.3633407665701572E-4	0.0	0.0	8.45066774158512	4.544469221900524E-5
60	1.8177876887602096E-4	0.0	0.0	8.68411712551415	4.544469221900524E-5
61	1.8177876887602096E-4	0.0	0.0	8.92165653174289	4.544469221900524E-5
62	1.8177876887602096E-4	0.0	0.0	9.200232495045393	4.544469221900524E-5
63	1.8177876887602096E-4	0.0	0.0	9.454495548010726	4.544469221900524E-5
64	1.8177876887602096E-4	0.0	0.0	9.698851658072318	4.544469221900524E-5
65	1.8177876887602096E-4	0.0	0.0	9.928892690084922	4.544469221900524E-5
66	1.8177876887602096E-4	0.0	0.0	10.146527321121738	4.544469221900524E-5
67	2.272234610950262E-4	0.0	0.0	10.388338528419066	4.544469221900524E-5
68	2.272234610950262E-4	0.0	0.0	10.678321109468538	4.544469221900524E-5
69	2.272234610950262E-4	0.0	0.0	11.098366399648803	4.544469221900524E-5
70	2.272234610950262E-4	0.0	0.0	11.36208194859569	4.544469221900524E-5
71	2.7266815331403145E-4	0.0	0.0	11.611709642954686	4.544469221900524E-5
72	3.181128455330367E-4	0.0	0.0	11.871607837755178	4.544469221900524E-5
73	3.181128455330367E-4	0.0	0.0	12.12591633541273	4.544469221900524E-5
74	3.181128455330367E-4	0.0	0.0	12.41830748514981	4.544469221900524E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	225	0.0	69.333336	2
CGTTTTT	6145	0.0	69.25956	1
ACGGGAT	1230	0.0	67.31708	5
TAGGGAC	2585	0.0	66.38298	5
CGAGGGA	1420	0.0	65.633804	4
CGTTAGG	430	0.0	65.11628	2
TAGCACG	80	0.0	65.0	1
TAAGGGA	3355	0.0	63.666172	4
TAACGGG	715	0.0	63.216785	3
GGCGATT	380	0.0	63.157894	8
ATAGGGA	3485	0.0	62.898132	4
TACGGGA	1000	0.0	62.4	4
AGCCGTA	180	0.0	62.22222	80
GTACGGG	795	0.0	61.88679	3
CACGGGA	590	0.0	61.694916	4
CGGGATC	740	0.0	61.62162	6
CCGATCC	2060	0.0	60.582523	80
GCGCGAC	40	2.977249E-7	60.0	9
TAGGGAG	3030	0.0	59.933994	5
TAGCGGG	1290	0.0	59.844963	3
>>END_MODULE