FastQCFastQC Report
Thu 26 May 2016
SRR1547799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547799_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2454908
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133200.5425865246273994No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG26950.10978008137168481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1150.073.043485
CGGGATA7500.068.266676
ACTACGG2200.067.272732
CGTTTTT68700.066.4921
ACGGGAT15450.066.278325
TTAGGCG1450.066.2068941
CGAGGGA15550.065.594864
TTGCGGG20750.064.771093
TACGGGA12500.064.644
GTACGGG11100.063.4234283
AGGGATG41200.063.300976
TAAGGGA38400.062.9166644
GAGTACG459.429641E-962.2222251
TGTAGGG39900.062.055143
AAGGGAT45650.061.949625
GAGGGAT49950.061.90195
TAATGCG2400.061.6666641
AGGGATT52450.061.6205946
CTAGACG650.061.538461
AGGGATC38400.060.6249966
Produced by FastQC (version 0.11.5)