##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547799_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2454908
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83752792365335	31.0	31.0	33.0	30.0	34.0
2	32.293999612205425	33.0	31.0	34.0	31.0	34.0
3	32.44424597581661	34.0	31.0	34.0	31.0	34.0
4	36.03340410312729	37.0	35.0	37.0	35.0	37.0
5	35.98270892432629	37.0	35.0	37.0	35.0	37.0
6	36.0272727124601	37.0	35.0	37.0	35.0	37.0
7	36.25602385099564	37.0	35.0	37.0	35.0	37.0
8	36.20556086012184	37.0	36.0	37.0	35.0	37.0
9	38.08106291559602	39.0	39.0	39.0	37.0	39.0
10-11	37.62717482691816	39.0	37.5	39.0	35.0	39.0
12-13	36.88776687354475	39.0	35.0	39.0	33.5	39.0
14-15	37.91803256985598	40.0	36.0	41.0	33.0	41.0
16-17	38.107449444133955	40.0	35.5	41.0	34.0	41.0
18-19	38.01269986492366	40.0	36.0	41.0	34.0	41.0
20-21	37.82448079520699	40.0	35.0	41.0	34.0	41.0
22-23	37.64954796676698	39.5	35.0	41.0	33.0	41.0
24-25	37.5623108890435	39.0	35.0	41.0	33.0	41.0
26-27	37.52985549763983	39.0	35.0	41.0	33.0	41.0
28-29	37.5374696322632	40.0	35.0	41.0	33.0	41.0
30-31	37.36059416483225	39.0	35.0	41.0	33.0	41.0
32-33	37.05154938596477	39.0	35.0	41.0	32.5	41.0
34-35	36.780438004194046	39.0	35.0	41.0	31.5	41.0
36-37	36.59557975288687	39.0	35.0	41.0	31.0	41.0
38-39	36.50769927019668	39.0	35.0	41.0	31.0	41.0
40-41	36.35550313901784	39.0	35.0	41.0	30.5	41.0
42-43	36.29958230613937	39.0	35.0	41.0	30.0	41.0
44-45	36.21705925435902	38.5	35.0	41.0	30.0	41.0
46-47	36.12453908659714	38.0	35.0	41.0	30.0	41.0
48-49	36.022700036009496	38.0	35.0	40.5	30.0	41.0
50-51	35.861663655012734	38.0	35.0	40.0	30.0	41.0
52-53	35.675297607894066	38.0	35.0	40.0	29.0	41.0
54-55	35.47933303406889	37.0	35.0	40.0	29.0	41.0
56-57	35.18819585092395	37.0	34.5	40.0	28.5	41.0
58-59	34.89380437067295	36.0	34.0	40.0	27.5	41.0
60-61	34.57502399275248	36.0	34.0	39.0	26.5	41.0
62-63	34.20673259445975	35.0	33.5	39.0	26.0	41.0
64-65	33.874707932028414	35.0	33.0	39.0	26.0	40.0
66-67	33.60109156840093	35.0	33.0	38.0	25.5	40.0
68-69	33.19422601580182	35.0	33.0	37.0	24.5	40.0
70-71	32.9464672403202	35.0	33.0	37.0	24.0	39.0
72-73	32.67813478142561	35.0	33.0	36.0	24.0	39.0
74-75	32.39006349728788	35.0	33.0	36.0	23.5	38.5
76-77	30.33832815730773	33.0	29.5	34.5	19.5	36.0
78-79	31.460339450602632	34.0	31.5	35.0	20.0	37.0
80-81	31.685939758231264	35.0	32.0	35.0	20.0	37.0
82-83	31.527792487539248	35.0	32.0	35.0	20.0	36.0
84-85	31.327638754690604	35.0	32.0	35.0	19.5	36.0
86	30.91348677832326	34.0	31.0	35.0	19.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	22.0
10	23.0
11	27.0
12	43.0
13	120.0
14	388.0
15	1078.0
16	2479.0
17	5675.0
18	11448.0
19	16832.0
20	19227.0
21	19314.0
22	16664.0
23	14986.0
24	15753.0
25	18126.0
26	21724.0
27	26638.0
28	33141.0
29	41904.0
30	52309.0
31	65807.0
32	83088.0
33	112048.0
34	268541.0
35	245246.0
36	218122.0
37	372709.0
38	516339.0
39	253995.0
40	1081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.695159248330285	23.746307397262953	30.54863970462437	14.009893649782395
2	31.687541854928984	25.297200546822936	29.844988081019736	13.170269517228345
3	28.750527514676723	25.576355610882363	32.426347545407	13.246769329033919
4	27.3064815463553	26.98769159577467	31.980628194620735	13.725198663249294
5	25.397448702761977	31.275673059845825	28.901571871532454	14.425306365859738
6	22.518685017931425	39.68148704554305	26.545638370154805	11.254189566370714
7	87.15401962110188	3.9472762319402603	7.112201353370473	1.786502793587377
8	87.41484405932931	3.8202246275624177	6.689089774443686	2.0758415386645854
9	82.61621209430251	6.269033299822234	8.35391794723061	2.760836658644642
10-11	38.55480531245977	28.97752176456307	20.70201816116938	11.765654761807774
12-13	29.447152398379085	25.350624137442217	28.46982453110259	16.732398933076105
14-15	16.343280481386675	31.390137634485693	35.68687706423214	16.57970481989549
16-17	21.422778368883886	25.679903279471166	33.193463869114446	19.703854482530506
18-19	23.448536564303023	27.51382129187733	30.721904853460902	18.31573729035874
20-21	25.419913903087206	27.40096573883828	30.399367308265727	16.77975304980879
22-23	20.9935973160705	24.373540678510153	32.84031417877982	21.792547826639534
24-25	19.623158994145605	27.8271324220704	33.75083709857966	18.798871485204334
26-27	17.855903357681836	32.79206389811757	30.507171755519963	18.844860988680633
28-29	16.303706696951576	28.963386815310386	35.642211439288154	19.09069504844988
30-31	22.473388004764335	28.339819659229594	31.043138887485807	18.14365344852027
32-33	25.255752965080564	27.861003345135543	28.22851202570524	18.654731664078653
34-35	19.822473999025625	28.362794043605703	31.18701393290502	20.627718024463647
36-37	22.801669146053538	28.346601990787434	30.546317825352315	18.305411037806714
38-39	19.609798004650276	30.20155948817634	29.18915902347461	20.99948348369878
40-41	21.08857032524233	26.82224751395979	29.764374062082975	22.3248080987149
42-43	20.438423761705124	26.256279257715565	30.16414464411701	23.141152336462305
44-45	19.472379412996332	30.70658859721016	29.19060510617913	20.630426883614376
46-47	20.612014788334225	29.909552618672468	29.00410117201948	20.474331420973822
48-49	21.224766875174144	27.309170038144	31.313311944887545	20.152751141794315
50-51	19.24935679870692	30.684347437867327	29.899755917533366	20.16653984589239
52-53	22.30437963459323	26.568286876738355	30.296267721641705	20.831065767026708
54-55	21.500398385601414	27.123684472086122	30.83838579694229	20.537531345370173
56-57	21.77118246386423	25.98119766606325	30.141536872257536	22.106082997814987
58-59	22.831385127263427	28.006691085775927	28.83395222957439	20.327971557386267
60-61	23.61218831825877	29.140012578882796	27.961475542056974	19.286323560801463
62-63	22.94507574214594	26.03792076933229	30.679662944599144	20.337340543922625
64-65	20.196724276429094	29.726653707593115	30.794168253963083	19.282453762014708
66-67	19.330357797522353	29.17441305336086	27.5662061470328	23.929023002083987
68-69	18.241498255739117	30.907981073017808	27.718228137266244	23.132292533976834
70-71	19.60330081616093	29.275374066971143	29.730645710552082	21.390679406315837
72-73	21.963307789945695	29.921304586566993	28.53565184520153	19.579735778285784
74-75	21.270552705030088	30.941546485652417	26.290679732193627	21.497221077123868
76-77	24.338101468568272	28.44668720783019	27.646148043022382	19.56906328057915
78-79	19.416430269484643	29.34659873200951	30.428390799166404	20.808580199339445
80-81	17.68190498381202	28.358761305922663	31.398019803593453	22.56131390667186
82-83	19.354818999327062	27.233118308303204	31.820438892211033	21.5916238001587
84-85	17.636404297024573	27.639406446188612	30.15799370078227	24.56619555600454
86	18.778341184272488	26.72149017396986	32.12010388984027	22.380064751917384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1586.0
1	2139.5
2	5636.5
3	5666.0
4	3194.5
5	4167.0
6	4987.0
7	5277.0
8	5363.0
9	5507.0
10	5636.0
11	5569.0
12	5521.5
13	5712.5
14	5813.0
15	5767.5
16	5677.5
17	5820.0
18	6232.5
19	7224.0
20	7901.0
21	7812.0
22	8246.0
23	9821.5
24	12284.5
25	15460.0
26	19530.5
27	24870.5
28	30308.5
29	32623.0
30	38098.5
31	45389.0
32	49841.0
33	53425.0
34	56700.5
35	63747.0
36	68465.0
37	70351.0
38	74006.0
39	77828.5
40	89547.5
41	104926.5
42	115792.0
43	120944.0
44	127711.5
45	135288.0
46	134445.5
47	136359.0
48	135639.0
49	121374.5
50	111395.0
51	98228.0
52	79507.0
53	70038.0
54	63432.0
55	57770.0
56	52148.5
57	49498.0
58	44372.0
59	36175.0
60	28872.5
61	21051.5
62	15168.5
63	11269.0
64	9663.0
65	8268.5
66	6053.0
67	4123.5
68	2863.5
69	2335.5
70	1638.5
71	1318.0
72	999.5
73	459.5
74	175.5
75	155.0
76	128.0
77	46.0
78	29.5
79	26.0
80	21.5
81	13.5
82	14.5
83	14.5
84	9.5
85	8.0
86	7.0
87	4.5
88	2.5
89	2.0
90	1.0
91	1.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2454908.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.828279224591512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77180664289072	12.626504297937984
2	8.593819361852542	2.7205074493020573
3	2.6495782317009704	1.2581479223628715
4	1.3165375601413196	0.83354096446317
5	0.7807439917807464	0.6178916952413918
6	0.5274105829171675	0.5008801183450501
7	0.42869470726033504	0.47498496700347714
8	0.31767233388056837	0.4022565122071439
9	0.2618063599073433	0.37295497506485975
>10	2.526944495421598	9.023370186765032
>50	0.9657577029222412	11.217892461770704
>100	1.8444619714087118	57.33593225842337
>500	0.010621199553472986	1.0715095949412419
>1k	0.0038858047146852386	0.9974568500895703
>5k	0.0	0.0
>10k+	2.590536476456826E-4	0.5461697460820963
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13320	0.5425865246273994	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG	2695	0.10978008137168481	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.073472407112609E-5	0.0	0.0	0.03474671963267055	0.0
2	4.073472407112609E-5	0.0	0.0	0.1446082704524976	0.0
3	4.073472407112609E-5	0.0	0.0	0.2130018721679183	0.0
4	4.073472407112609E-5	0.0	0.0	0.3235966480210256	0.0
5	4.073472407112609E-5	0.0	0.0	0.5625058046981801	0.0
6	4.073472407112609E-5	0.0	0.0	0.7462194102589588	0.0
7	4.073472407112609E-5	0.0	0.0	0.8568956555602084	0.0
8	4.073472407112609E-5	0.0	0.0	1.0610580926046924	0.0
9	4.073472407112609E-5	0.0	0.0	1.1366210057566313	0.0
10	4.073472407112609E-5	0.0	0.0	1.3084808066127123	0.0
11	4.073472407112609E-5	0.0	0.0	1.591790812527394	0.0
12	4.073472407112609E-5	0.0	0.0	1.81917204229242	0.0
13	4.073472407112609E-5	0.0	0.0	1.898686223679258	0.0
14	4.073472407112609E-5	0.0	0.0	1.9380359671319658	0.0
15	4.073472407112609E-5	0.0	0.0	1.9946979683149022	0.0
16	4.073472407112609E-5	0.0	0.0	2.114091444567373	0.0
17	4.073472407112609E-5	0.0	0.0	2.259229266432795	0.0
18	4.073472407112609E-5	0.0	0.0	2.4179317514139025	0.0
19	4.073472407112609E-5	0.0	0.0	2.536673472081235	0.0
20	4.073472407112609E-5	0.0	0.0	2.6636028722868637	0.0
21	4.073472407112609E-5	0.0	0.0	2.8126104929390428	0.0
22	4.073472407112609E-5	0.0	0.0	2.9746124905699114	0.0
23	4.073472407112609E-5	0.0	0.0	3.1264715419070694	0.0
24	4.073472407112609E-5	0.0	0.0	3.2453762014706866	0.0
25	4.073472407112609E-5	0.0	0.0	3.3505125242982627	0.0
26	4.073472407112609E-5	0.0	0.0	3.456830154123902	0.0
27	4.073472407112609E-5	0.0	0.0	3.585388943292376	0.0
28	4.073472407112609E-5	0.0	0.0	3.704619480648562	0.0
29	4.073472407112609E-5	0.0	0.0	3.8549306124710174	0.0
30	4.073472407112609E-5	0.0	0.0	4.012777668246631	0.0
31	8.146944814225218E-5	0.0	0.0	4.160603981900747	0.0
32	8.146944814225218E-5	0.0	0.0	4.287777790450803	0.0
33	8.146944814225218E-5	0.0	0.0	4.422935604918799	0.0
34	8.146944814225218E-5	0.0	0.0	4.5709248574692	0.0
35	8.146944814225218E-5	0.0	0.0	4.737815021988604	0.0
36	8.146944814225218E-5	0.0	0.0	4.882219618820746	0.0
37	8.146944814225218E-5	0.0	0.0	5.038885367598297	0.0
38	1.2220417221337827E-4	0.0	0.0	5.208871371147106	0.0
39	1.2220417221337827E-4	0.0	0.0	5.523180502079915	0.0
40	1.2220417221337827E-4	0.0	0.0	5.7000099392726735	0.0
41	1.2220417221337827E-4	0.0	0.0	5.862460018868324	0.0
42	1.2220417221337827E-4	0.0	0.0	6.039452394957367	0.0
43	1.2220417221337827E-4	0.0	0.0	6.210578970780167	0.0
44	1.2220417221337827E-4	0.0	0.0	6.374862112959019	0.0
45	1.6293889628450436E-4	0.0	0.0	6.541548603858067	0.0
46	1.6293889628450436E-4	0.0	0.0	6.722003431493156	0.0
47	1.6293889628450436E-4	0.0	0.0	6.911419898423892	0.0
48	1.6293889628450436E-4	0.0	0.0	7.095296442880955	0.0
49	1.6293889628450436E-4	0.0	0.0	7.289478872528013	0.0
50	1.6293889628450436E-4	0.0	0.0	7.479669299216101	0.0
51	1.6293889628450436E-4	0.0	0.0	7.688190351736195	0.0
52	1.6293889628450436E-4	0.0	0.0	7.935816739364571	0.0
53	1.6293889628450436E-4	0.0	0.0	8.13729068462036	0.0
54	1.6293889628450436E-4	0.0	0.0	8.35212561937148	0.0
55	1.6293889628450436E-4	0.0	0.0	8.558121118999164	0.0
56	1.6293889628450436E-4	0.0	0.0	8.77136739951151	0.0
57	1.6293889628450436E-4	0.0	0.0	8.98082535068524	0.0
58	1.6293889628450436E-4	0.0	0.0	9.18735040172585	0.0
59	1.6293889628450436E-4	0.0	0.0	9.409802729878269	0.0
60	1.6293889628450436E-4	0.0	0.0	9.668916309694701	0.0
61	1.6293889628450436E-4	0.0	0.0	9.929170461785127	0.0
62	1.6293889628450436E-4	0.0	0.0	10.238876568897897	0.0
63	2.0367362035563044E-4	0.0	0.0	10.518316776025822	0.0
64	2.0367362035563044E-4	0.0	0.0	10.788550935513673	0.0
65	2.0367362035563044E-4	0.0	0.0	11.049090230672595	0.0
66	2.0367362035563044E-4	0.0	0.0	11.278915543881888	0.0
67	2.0367362035563044E-4	0.0	0.0	11.527519564887971	0.0
68	2.0367362035563044E-4	0.0	0.0	11.817387861378105	0.0
69	2.4440834442675653E-4	0.0	0.0	12.27740510031333	0.0
70	2.4440834442675653E-4	0.0	0.0	12.550001873797306	0.0
71	2.4440834442675653E-4	0.0	0.0	12.811152189817296	0.0
72	2.4440834442675653E-4	0.0	0.0	13.070387973805943	0.0
73	2.4440834442675653E-4	0.0	0.0	13.342495930601066	0.0
74	2.4440834442675653E-4	0.0	0.0	13.643688480382972	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	115	0.0	73.04348	5
CGGGATA	750	0.0	68.26667	6
ACTACGG	220	0.0	67.27273	2
CGTTTTT	6870	0.0	66.492	1
ACGGGAT	1545	0.0	66.27832	5
TTAGGCG	145	0.0	66.206894	1
CGAGGGA	1555	0.0	65.59486	4
TTGCGGG	2075	0.0	64.77109	3
TACGGGA	1250	0.0	64.64	4
GTACGGG	1110	0.0	63.423428	3
AGGGATG	4120	0.0	63.30097	6
TAAGGGA	3840	0.0	62.916664	4
GAGTACG	45	9.429641E-9	62.222225	1
TGTAGGG	3990	0.0	62.05514	3
AAGGGAT	4565	0.0	61.94962	5
GAGGGAT	4995	0.0	61.9019	5
TAATGCG	240	0.0	61.666664	1
AGGGATT	5245	0.0	61.620594	6
CTAGACG	65	0.0	61.53846	1
AGGGATC	3840	0.0	60.624996	6
>>END_MODULE