FastQCFastQC Report
Thu 26 May 2016
SRR1547798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547798_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2498952
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105670.4228572617641316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58050.069.112831
CCGATCC15400.066.23376580
GTTAGCG3050.065.573771
ACGTCGA559.094947E-1265.4545449
GGGCGAT13450.065.427517
ACGGGAT16950.065.368735
AGTAGCG1850.064.864861
AGGGATT54100.064.3253256
CGATAGG5450.063.8532072
TACGGGA11850.063.797474
TAAGGGA36350.063.6038484
TCGCGCG700.062.8571431
CGTTAGG5250.062.476192
ATAGGGA42150.062.2538534
ACGTAGG3100.061.9354822
TAGGGAC29900.061.8060265
TTAGGGA37650.060.9827354
TAACGGG8500.060.7058833
TAGGGAT54250.060.460835
CGTATGG3050.060.327872
Produced by FastQC (version 0.11.5)