##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547798_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2498952
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80987469947402	31.0	31.0	33.0	30.0	34.0
2	32.262912612967355	33.0	31.0	34.0	31.0	34.0
3	32.42566924054564	34.0	31.0	34.0	31.0	34.0
4	36.01726643809085	37.0	35.0	37.0	35.0	37.0
5	35.96598894256472	37.0	35.0	37.0	35.0	37.0
6	36.01447726887111	37.0	35.0	37.0	35.0	37.0
7	36.243070695235446	37.0	35.0	37.0	35.0	37.0
8	36.20481745947902	37.0	36.0	37.0	35.0	37.0
9	38.082887546459474	39.0	39.0	39.0	37.0	39.0
10-11	37.571772086858815	39.0	37.5	39.0	35.0	39.0
12-13	36.830480937609046	39.0	35.0	39.0	33.0	39.0
14-15	37.91968213074921	40.0	35.0	41.0	33.0	41.0
16-17	38.083079226811876	40.0	35.0	41.0	34.0	41.0
18-19	37.992311176845334	40.0	36.0	41.0	34.0	41.0
20-21	37.82195736452721	40.0	35.0	41.0	33.5	41.0
22-23	37.629058501323755	39.5	35.0	41.0	33.0	41.0
24-25	37.56563791541414	39.0	35.0	41.0	33.0	41.0
26-27	37.561736079764636	39.5	35.0	41.0	33.0	41.0
28-29	37.56139933860274	40.0	35.0	41.0	33.0	41.0
30-31	37.41088704384878	40.0	35.0	41.0	33.0	41.0
32-33	37.15364900966485	39.5	35.0	41.0	32.5	41.0
34-35	36.925541987201036	39.0	35.0	41.0	32.0	41.0
36-37	36.75721742554479	39.0	35.0	41.0	31.0	41.0
38-39	36.67859166562623	39.0	35.0	41.0	31.0	41.0
40-41	36.54871041940781	39.0	35.0	41.0	31.0	41.0
42-43	36.50159566890441	39.0	35.0	41.0	31.0	41.0
44-45	36.386908191914046	39.0	35.0	41.0	31.0	41.0
46-47	36.29932007497543	38.0	35.0	41.0	31.0	41.0
48-49	36.187956191235365	38.0	35.0	40.5	30.5	41.0
50-51	36.03123929551268	38.0	35.0	40.0	30.0	41.0
52-53	35.85522030835327	38.0	35.0	40.0	30.0	41.0
54-55	35.66593636052233	37.0	35.0	40.0	29.5	41.0
56-57	35.3646202488083	37.0	34.5	40.0	29.0	41.0
58-59	35.07514089906489	36.0	34.0	40.0	28.0	41.0
60-61	34.788577571718065	36.0	34.0	39.5	27.5	41.0
62-63	34.410259380732406	35.5	33.5	39.0	27.0	41.0
64-65	34.152545347009465	35.0	34.0	39.0	26.0	40.0
66-67	33.88965434310063	35.0	34.0	38.0	26.0	40.0
68-69	33.507224628564295	35.0	33.5	37.5	25.5	40.0
70-71	33.28411290012774	35.0	33.0	37.0	26.0	39.5
72-73	33.041502197721286	35.0	33.0	36.5	25.5	39.0
74-75	32.73992837797605	35.0	33.0	36.0	25.0	39.0
76-77	30.66835497440527	33.0	30.0	34.5	21.0	36.0
78-79	31.78815559482535	34.5	31.5	35.0	23.0	37.0
80-81	32.03964661986305	35.0	32.5	35.0	24.0	37.0
82-83	31.89229945193025	35.0	32.5	35.0	24.0	36.0
84-85	31.700753755974503	35.0	32.0	35.0	23.5	36.0
86	31.28240718509199	34.0	31.0	35.0	20.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	16.0
10	24.0
11	44.0
12	62.0
13	143.0
14	314.0
15	872.0
16	2063.0
17	4527.0
18	8855.0
19	13786.0
20	16485.0
21	17509.0
22	16247.0
23	15185.0
24	15821.0
25	17950.0
26	21592.0
27	26631.0
28	32942.0
29	41072.0
30	51814.0
31	64877.0
32	81775.0
33	111967.0
34	270344.0
35	255095.0
36	218873.0
37	381561.0
38	532309.0
39	277035.0
40	1155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.07288495337245	24.39578671379042	30.524395826730565	13.006932506106562
2	30.364128642727028	25.325016246810662	31.366548857280975	12.944306253181335
3	28.84525192960889	25.685607406624854	31.363347515278406	14.105793148487846
4	27.15634393937939	27.117567684373288	30.82728279694848	14.898805579298843
5	24.825406810534975	31.668795559098374	29.64258617212335	13.863211458243295
6	23.659558086749964	39.79612253456649	25.823985414685836	10.720333963997708
7	89.00399047280621	3.678662095150287	5.6512490035823015	1.6660984284612108
8	89.78443763625712	3.034552084233711	5.447683668994043	1.733326610515128
9	85.13604903175411	6.15986221424021	6.359225787450098	2.34486296655558
10-11	38.95651056923062	29.776362251055644	19.718546014489274	11.548581165224462
12-13	29.737646021212093	25.911582135231086	27.85701766180383	16.49375418175299
14-15	16.39683355262526	32.03420874030394	34.7051083814335	16.863849325637307
16-17	21.983635540018376	26.35426770902362	32.33885644862327	19.32324030233474
18-19	23.756298640390053	28.436760690081282	29.977026369454073	17.82991430007459
20-21	26.847754578719403	28.582321709260523	28.434599784229547	16.13532392779053
22-23	21.568381465510342	25.42395772307751	31.229751511833758	21.777909299578383
24-25	19.38008413126783	28.03623278878506	33.88386411583736	18.699818964109756
26-27	17.803323153065765	34.80937208877962	29.135253498266472	18.252051259888145
28-29	16.550978170048886	29.51825405209864	35.2513773773966	18.67939040045587
30-31	21.961566288588177	29.114404758474755	30.844409976662217	18.079618976274855
32-33	24.761580054358788	28.709535037087548	28.30952735386674	18.219357554686923
34-35	19.7800517977136	28.663055552887773	30.28281455586182	21.27407809353681
36-37	22.03569736433513	29.986910512887	29.70587270183661	18.27151942094126
38-39	19.415979178471616	31.190154912939505	28.499707077206764	20.894158831382114
40-41	20.644934356482235	27.162666589834462	29.3985038528151	22.793895200868203
42-43	20.59191212956471	26.904078189577067	29.070426322714482	23.433583358143732
44-45	18.79661954291239	31.590162596160308	28.869342028178213	20.74387583274909
46-47	20.810343696077396	30.702890651761216	27.86628154522376	20.620484106937628
48-49	20.89417883976963	27.223372037558146	31.52295442249391	20.359494700178317
50-51	19.247488547198984	31.158301560013957	29.090654802493205	20.50355509029385
52-53	21.69867608501484	27.124370536128744	29.976366092666044	21.20058728619037
54-55	21.70129718377944	27.993194747238043	30.356805572896157	19.94870249608636
56-57	22.520440568686393	26.079592565203335	29.062903169008447	22.337063697101826
58-59	23.008365106652708	27.589685596201928	28.70163172401871	20.700317573126657
60-61	23.59797226997557	29.11808630177771	27.496406493602116	19.7875349346446
62-63	22.52612295074095	26.923546350630183	30.660532895389746	19.88979780323912
64-65	19.534008656428774	30.267968332324912	30.3608472671744	19.837175744071914
66-67	18.95006386677295	29.47921768805483	26.630643565782776	24.94007487938944
68-69	18.175038976338882	31.596765364040607	26.409450841792882	23.818744817827632
70-71	19.32708191273782	29.91414000749114	29.17829153981349	21.58048653995755
72-73	21.848939075260347	30.17408897809962	27.53982469451194	20.437147252128092
74-75	21.065910829819863	31.612151814040445	25.622560977561793	21.6993763785779
76-77	24.69167074837772	28.590225022329363	26.50431060700646	20.213793622286463
78-79	19.061890744600134	29.29940230944812	30.173688810349297	21.465018135602445
80-81	16.947784511267123	28.900355028828084	30.680121106767956	23.471739353136837
82-83	18.910167142065955	27.54520695075375	31.01454129571116	22.530084611469128
84-85	17.33944869689374	27.442283805371208	29.64194590372284	25.576321594012207
86	18.13532232711953	27.49944776850456	31.098276397465817	23.266953506910095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1364.0
1	1822.5
2	4490.0
3	4532.5
4	2483.0
5	3141.0
6	3994.5
7	4307.0
8	4483.5
9	4653.0
10	4625.0
11	4497.5
12	4362.0
13	4487.0
14	4641.0
15	4662.5
16	4806.5
17	5185.0
18	5337.0
19	5843.5
20	6495.5
21	6537.0
22	6973.5
23	8636.5
24	11358.0
25	13868.5
26	17964.0
27	23776.0
28	32349.5
29	38191.0
30	41413.0
31	45587.0
32	51847.5
33	56451.0
34	61412.0
35	68512.0
36	69946.0
37	72693.0
38	78618.5
39	85328.5
40	92634.5
41	104366.0
42	119624.5
43	126926.0
44	133049.5
45	137196.0
46	137411.5
47	143469.5
48	145850.5
49	130440.5
50	116515.0
51	99207.0
52	79727.5
53	71207.5
54	63642.0
55	60532.5
56	52527.5
57	46415.0
58	41998.0
59	32645.0
60	23973.0
61	18938.0
62	16282.5
63	12708.0
64	10490.0
65	8344.5
66	5227.5
67	3395.5
68	2257.5
69	1695.0
70	1165.5
71	921.0
72	714.0
73	527.0
74	372.5
75	138.5
76	73.5
77	63.0
78	54.0
79	50.0
80	35.5
81	16.5
82	18.0
83	18.5
84	7.5
85	4.5
86	7.0
87	5.5
88	5.0
89	4.5
90	2.5
91	1.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2498952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.97096608788996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4125192801224	12.0385309918451
2	8.384034013535512	2.510341777927122
3	2.5217826616977286	1.1326056812791674
4	1.1653441642587283	0.6978531185535155
5	0.6853931301668548	0.5130498654300366
6	0.5134581080118932	0.46121783535589156
7	0.3441342506790559	0.36064155376182977
8	0.2765845186477268	0.3312589959288389
9	0.21003828095996763	0.28300283832693407
>10	2.311022367118548	8.19286567882794
>50	1.0847056619874857	11.954464828498445
>100	2.0775089941663607	59.49644425683622
>500	0.009971180799397095	0.9619568517805445
>1k	0.0032338964754801383	0.6394352281118085
>5k	0.0	0.0
>10k+	2.6949137295667825E-4	0.42633049753659413
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10567	0.4228572617641316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.001677503209345E-5	0.0	0.0	0.0311730677500008	0.0
2	4.001677503209345E-5	0.0	0.0	0.10876559453723	0.0
3	4.001677503209345E-5	0.0	0.0	0.1615077040295292	0.0
4	4.001677503209345E-5	0.0	0.0	0.24786390454878685	0.0
5	4.001677503209345E-5	0.0	0.0	0.4358226968745298	0.0
6	4.001677503209345E-5	0.0	0.0	0.5837246973931473	0.0
7	4.001677503209345E-5	0.0	0.0	0.6793647897198506	0.0
8	4.001677503209345E-5	0.0	0.0	0.8699646891977116	0.0
9	4.001677503209345E-5	0.0	0.0	0.9293896001203704	0.0
10	4.001677503209345E-5	0.0	0.0	1.0447179457628637	0.0
11	4.001677503209345E-5	0.0	0.0	1.2451219551235877	0.0
12	4.001677503209345E-5	0.0	0.0	1.39846623704657	0.0
13	4.001677503209345E-5	0.0	0.0	1.4519286484894467	0.0
14	4.001677503209345E-5	0.0	0.0	1.4748982773578685	0.0
15	4.001677503209345E-5	0.0	0.0	1.514995085940026	0.0
16	4.001677503209345E-5	0.0	0.0	1.5963892063553042	0.0
17	4.001677503209345E-5	0.0	0.0	1.7045545492670529	0.0
18	4.001677503209345E-5	0.0	0.0	1.823284320787274	0.0
19	4.001677503209345E-5	0.0	0.0	1.9071594812545418	0.0
20	4.001677503209345E-5	0.0	0.0	1.9987178625279718	0.0
21	4.001677503209345E-5	0.0	0.0	2.1124855539442136	0.0
22	4.001677503209345E-5	0.0	0.0	2.2434204418492234	0.0
23	4.001677503209345E-5	0.0	0.0	2.376116067855645	0.0
24	4.001677503209345E-5	0.0	0.0	2.476998357711553	0.0
25	4.001677503209345E-5	0.0	0.0	2.566155732483057	0.0
26	4.001677503209345E-5	0.0	0.0	2.6537524530283094	0.0
27	4.001677503209345E-5	0.0	0.0	2.750392964730815	0.0
28	4.001677503209345E-5	0.0	0.0	2.8525557913877497	0.0
29	4.001677503209345E-5	0.0	0.0	2.9774081294878814	0.0
30	4.001677503209345E-5	0.0	0.0	3.116306355624278	0.0
31	4.001677503209345E-5	0.0	0.0	3.249642250031213	0.0
32	4.001677503209345E-5	0.0	0.0	3.3636900588726792	0.0
33	4.001677503209345E-5	0.0	0.0	3.4788983541900764	0.0
34	4.001677503209345E-5	0.0	0.0	3.604951195541171	0.0
35	4.001677503209345E-5	0.0	0.0	3.753573498010366	0.0
36	4.001677503209345E-5	0.0	0.0	3.8830277652391882	0.0
37	4.001677503209345E-5	0.0	0.0	4.026167769528986	0.0
38	4.001677503209345E-5	0.0	0.0	4.179432017901904	0.0
39	4.001677503209345E-5	0.0	0.0	4.381236614388752	0.0
40	4.001677503209345E-5	0.0	0.0	4.5445850900697575	0.0
41	4.001677503209345E-5	0.0	0.0	4.719418380184973	0.0
42	4.001677503209345E-5	0.0	0.0	4.888729355345761	0.0
43	4.001677503209345E-5	0.0	0.0	5.046235381872081	0.0
44	4.001677503209345E-5	0.0	0.0	5.210504243378824	0.0
45	4.001677503209345E-5	0.0	0.0	5.369050706055979	0.0
46	4.001677503209345E-5	0.0	0.0	5.536240792140065	0.0
47	4.001677503209345E-5	0.0	0.0	5.719797739212278	0.0
48	4.001677503209345E-5	0.0	0.0	5.904715256635582	0.0
49	4.001677503209345E-5	0.0	0.0	6.099717001366973	0.0
50	4.001677503209345E-5	0.0	0.0	6.299320675227055	0.0
51	4.001677503209345E-5	0.0	0.0	6.488720071453954	0.0
52	4.001677503209345E-5	0.0	0.0	6.709772736731238	0.0
53	4.001677503209345E-5	0.0	0.0	6.907135471189522	0.0
54	4.001677503209345E-5	0.0	0.0	7.1164632213824035	0.0
55	4.001677503209345E-5	0.0	0.0	7.320668824371176	0.0
56	4.001677503209345E-5	0.0	0.0	7.535839023718743	0.0
57	8.00335500641869E-5	0.0	0.0	7.743886237110597	0.0
58	8.00335500641869E-5	0.0	0.0	7.9527738027781245	0.0
59	8.00335500641869E-5	0.0	0.0	8.16926455570175	0.0
60	8.00335500641869E-5	0.0	0.0	8.404643226440523	0.0
61	1.2005032509628036E-4	0.0	0.0	8.640382048154587	0.0
62	1.2005032509628036E-4	0.0	0.0	8.913976739049009	0.0
63	1.2005032509628036E-4	0.0	0.0	9.181008678838168	0.0
64	1.2005032509628036E-4	0.0	0.0	9.443238605623478	0.0
65	1.2005032509628036E-4	0.0	0.0	9.694784053475217	0.0
66	1.2005032509628036E-4	0.0	0.0	9.949850977529781	0.0
67	1.600671001283738E-4	0.0	0.0	10.199875787930301	0.0
68	1.600671001283738E-4	0.0	0.0	10.46862844904584	0.0
69	1.600671001283738E-4	0.0	0.0	10.805329594165874	0.0
70	2.0008387516046728E-4	0.0	0.0	11.07380213785619	0.0
71	2.0008387516046728E-4	0.0	0.0	11.342434748646633	0.0
72	2.4010065019256072E-4	0.0	0.0	11.614908969840156	0.0
73	2.4010065019256072E-4	0.0	0.0	11.88334149675544	0.0
74	2.801174252246542E-4	0.0	0.0	12.180946252669118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5805	0.0	69.11283	1
CCGATCC	1540	0.0	66.233765	80
GTTAGCG	305	0.0	65.57377	1
ACGTCGA	55	9.094947E-12	65.454544	9
GGGCGAT	1345	0.0	65.42751	7
ACGGGAT	1695	0.0	65.36873	5
AGTAGCG	185	0.0	64.86486	1
AGGGATT	5410	0.0	64.325325	6
CGATAGG	545	0.0	63.853207	2
TACGGGA	1185	0.0	63.79747	4
TAAGGGA	3635	0.0	63.603848	4
TCGCGCG	70	0.0	62.857143	1
CGTTAGG	525	0.0	62.47619	2
ATAGGGA	4215	0.0	62.253853	4
ACGTAGG	310	0.0	61.935482	2
TAGGGAC	2990	0.0	61.806026	5
TTAGGGA	3765	0.0	60.982735	4
TAACGGG	850	0.0	60.705883	3
TAGGGAT	5425	0.0	60.46083	5
CGTATGG	305	0.0	60.32787	2
>>END_MODULE