FastQCFastQC Report
Thu 26 May 2016
SRR1547797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547797_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3236639
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153570.4744736746977343No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39440.1218548006126108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG550.072.727271
CGAGGGA28650.069.389184
CGTTAGG5300.068.6792452
CGATAGG5300.068.6792452
CAACGCG1050.068.5714341
CGTTTTT81150.068.022181
GTTTGCG2650.067.924521
AGGGATT77450.067.346676
ACGGGAT20350.067.223595
TAGGGAC43750.066.102865
TACGGGA17800.065.84274
TTAGGGA51750.064.772954
AGCGGGA24800.063.8709684
CGGGATA10050.063.681596
CGGGACG1450.063.448286
CGTAGGG16900.063.4319533
ACGTAGG7900.063.291142
GGGACCG16450.063.221897
AGGGATG61150.062.9926386
TAAGGGA54900.062.367944
Produced by FastQC (version 0.11.5)