##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547797_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3236639
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71993262146319	31.0	31.0	33.0	30.0	34.0
2	32.15683893075502	33.0	31.0	34.0	30.0	34.0
3	32.317189220052036	34.0	31.0	34.0	30.0	34.0
4	35.9152698215649	37.0	35.0	37.0	35.0	37.0
5	35.84794751592624	37.0	35.0	37.0	35.0	37.0
6	35.90892156956645	37.0	35.0	37.0	35.0	37.0
7	36.2140074935759	37.0	35.0	37.0	35.0	37.0
8	36.18026044918818	37.0	35.0	37.0	35.0	37.0
9	38.049611340653065	39.0	39.0	39.0	35.0	39.0
10-11	37.44651519678284	39.0	37.0	39.0	34.5	39.0
12-13	36.79956213837873	39.0	35.0	39.0	33.0	39.0
14-15	37.9071133666745	40.0	36.0	41.0	33.0	41.0
16-17	38.03959215099367	40.0	36.0	41.0	33.0	41.0
18-19	37.93899474114969	40.0	36.0	41.0	33.5	41.0
20-21	37.7819640991782	40.0	35.0	41.0	33.0	41.0
22-23	37.5758949329845	39.5	35.0	41.0	33.0	41.0
24-25	37.499802881940184	39.0	35.0	41.0	33.0	41.0
26-27	37.4761830095973	39.5	35.0	41.0	33.0	41.0
28-29	37.4745319141245	40.0	35.0	41.0	33.0	41.0
30-31	37.33102718591724	39.0	35.0	41.0	33.0	41.0
32-33	37.06803878962096	39.0	35.0	41.0	32.0	41.0
34-35	36.839570616309075	39.0	35.0	41.0	31.0	41.0
36-37	36.67417419736955	39.0	35.0	41.0	31.0	41.0
38-39	36.5797923401405	39.0	35.0	41.0	31.0	41.0
40-41	36.46444815130758	39.0	35.0	41.0	30.0	41.0
42-43	36.41228647989473	39.0	35.0	41.0	30.0	41.0
44-45	36.31701990861508	39.0	35.0	41.0	30.0	41.0
46-47	36.23517806588872	38.5	35.0	41.0	30.0	41.0
48-49	36.12018408602257	38.0	35.0	40.5	30.0	41.0
50-51	35.96079312521415	38.0	35.0	40.0	30.0	41.0
52-53	35.7758877341588	38.0	35.0	40.0	29.0	41.0
54-55	35.59026415982753	37.5	35.0	40.0	29.0	41.0
56-57	35.30924193275803	37.0	34.5	40.0	28.0	41.0
58-59	35.01982998412859	36.5	34.0	40.0	27.5	41.0
60-61	34.7168036039855	36.0	34.0	39.5	26.5	41.0
62-63	34.32489721590824	35.5	33.5	39.0	26.0	41.0
64-65	34.01009102343511	35.0	33.0	39.0	26.0	40.0
66-67	33.68945486351737	35.0	33.0	38.5	25.5	40.0
68-69	33.25352024121318	35.0	33.0	37.5	24.0	40.0
70-71	32.9689866556017	35.0	33.0	37.0	24.0	39.0
72-73	32.692341808894966	35.0	33.0	36.5	24.0	39.0
74-75	32.35828277419879	35.0	32.5	36.0	22.5	38.5
76-77	30.257489945588617	33.0	29.5	34.5	19.5	36.0
78-79	31.4009761360473	34.0	31.0	35.0	20.0	37.0
80-81	31.578903455096473	35.0	32.0	35.0	20.0	37.0
82-83	31.39045102033313	35.0	32.0	35.0	19.5	36.0
84-85	31.163580800948147	34.0	32.0	35.0	19.0	36.0
86	30.714168617507234	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	11.0
10	21.0
11	36.0
12	64.0
13	146.0
14	444.0
15	1164.0
16	2993.0
17	6371.0
18	11629.0
19	17774.0
20	21592.0
21	22550.0
22	21834.0
23	22265.0
24	23985.0
25	28008.0
26	33498.0
27	41356.0
28	49710.0
29	61504.0
30	75926.0
31	92763.0
32	113354.0
33	146860.0
34	323428.0
35	313791.0
36	281820.0
37	479962.0
38	691245.0
39	349228.0
40	1296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.50632492533149	23.65870892614221	31.90911930555122	12.925846842975075
2	29.1600947773292	25.492493911122	32.429443011716785	12.917968299832017
3	28.424424225253418	25.47661323984541	33.04378399938949	13.055178535511683
4	27.198399327203308	27.007120658188942	31.512040731141163	14.282439283466584
5	23.804322941174473	32.11300364359448	29.625299577740982	14.457373837490062
6	22.628658926744688	40.77643506118538	25.76382475771935	10.831081254350577
7	89.22567515252705	3.5590623483187347	5.589069401932066	1.6261930972221492
8	90.21435507636161	2.760085384869922	5.452137232480978	1.573422306287479
9	85.89598654653794	5.414505602880024	6.530323585670198	2.159184264911842
10-11	37.50192715344529	31.013977771385687	20.0622930144511	11.421802060717924
12-13	28.352065213327776	26.546565743043942	27.72113294068322	17.380236102945062
14-15	15.751308687808557	32.43753782859318	34.148989121122256	17.662164362476013
16-17	21.326922773902186	27.73046360746441	31.77799563065266	19.16461798798074
18-19	22.853320991312284	29.084460763155857	30.108841301115135	17.953376944416725
20-21	25.736497026699613	28.069503580720617	29.59872880478793	16.59527058779184
22-23	21.19023777443206	25.115945893255322	32.28809267885606	21.405723653456565
24-25	19.109406393484104	28.36080885140419	33.762847818369615	18.766936936742095
26-27	17.905904859948855	34.461890868892084	29.054074303621753	18.57812996753731
28-29	16.419192872606427	29.586370305739994	35.202489372463226	18.79194744919035
30-31	21.630478406767022	29.27325228423683	30.736606708378662	18.359662600617494
32-33	23.277495574884934	29.087735765403554	29.044481018735794	18.590287640975717
34-35	19.001748418652806	29.100001575708628	30.86026893947703	21.037981066161535
36-37	21.687682809235135	29.920018883786547	30.52050290440176	17.871795402576563
38-39	19.446453558768834	30.7678273665985	29.448990140698424	20.336728933934246
40-41	20.48676111237614	27.87595403750619	29.618471507017002	22.018813343100668
42-43	20.74754089041132	27.444626972609548	29.263937065579448	22.54389507139968
44-45	18.532357176688535	31.39083475172857	29.299807609066068	20.777000462516824
46-47	20.248551043227252	30.08298114185734	29.377233605601365	20.291234209314045
48-49	20.24448818666524	27.708990715368625	31.482905569635662	20.56361552833047
50-51	18.620844029871726	30.621626322861463	29.867309885347115	20.890219761919695
52-53	21.073666232162438	27.23723591046144	30.331386972720775	21.357710884655347
54-55	20.85475086965213	28.007293986138087	31.000383422432964	20.13757172177682
56-57	20.824240825127546	26.504485053785732	30.441377614247372	22.229896506839346
58-59	21.63743006248148	28.13517355503657	29.765506749439773	20.461889633042176
60-61	22.367106742519013	29.474062445641913	28.39850227350038	19.76032853833869
62-63	21.045241684352195	27.604484157794552	30.986093907908792	20.364180249944464
64-65	19.30714237825102	30.523654321658984	30.723058703797363	19.446144596292637
66-67	17.885899539615014	30.087399305266977	27.864553322134473	24.162147832983536
68-69	18.027095391237637	31.47785403314982	27.462639484971913	23.032411090640633
70-71	18.302612679387476	30.283003448948122	29.79003837004992	21.624345501614485
72-73	21.23753992953802	30.262179378052355	28.144689599303476	20.355591093106153
74-75	20.28159458005666	31.638221006420547	26.657313342637224	21.42287107088557
76-77	22.971282864724795	28.84458229663549	27.787513528694426	20.39662130994529
78-79	18.051503426857305	30.364121547073985	30.116271848667708	21.468103177401
80-81	16.818681354330835	29.150903143662298	30.93727783666946	23.09313766533741
82-83	18.673444891444486	28.188902129647452	31.00285512224255	22.13479785666551
84-85	16.766822002700952	28.148103634665468	30.231545748537293	24.85352861409629
86	18.06355296342904	28.38296146094761	30.824784599085657	22.7287009765377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2087.0
1	2560.5
2	6776.5
3	6905.0
4	3741.5
5	5063.0
6	6864.0
7	7794.0
8	8435.0
9	9118.5
10	9131.0
11	8626.0
12	7957.0
13	7637.5
14	7512.0
15	7538.5
16	7522.5
17	7450.0
18	7691.0
19	7644.5
20	8569.0
21	9811.0
22	9999.0
23	11815.0
24	15296.0
25	21861.5
26	29325.0
27	36467.5
28	41063.0
29	41267.0
30	45736.0
31	55029.0
32	63790.5
33	71293.0
34	80643.5
35	90572.0
36	94715.0
37	92980.0
38	101013.5
39	123170.0
40	138433.5
41	148920.0
42	161108.5
43	165686.0
44	177275.5
45	183588.5
46	180514.5
47	182636.0
48	177991.5
49	159154.0
50	144880.0
51	129399.0
52	106948.5
53	92963.0
54	79098.5
55	67356.5
56	59450.0
57	56437.0
58	50141.0
59	36877.5
60	24910.0
61	18211.5
62	14331.5
63	9864.0
64	7578.0
65	6297.5
66	3969.0
67	2600.0
68	1692.5
69	855.5
70	605.0
71	605.0
72	487.5
73	232.5
74	100.5
75	99.0
76	62.5
77	36.0
78	30.0
79	21.0
80	18.5
81	12.5
82	8.5
83	8.0
84	7.0
85	5.5
86	5.0
87	3.0
88	2.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3236639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.431385451759589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97431712027574	12.032517428207118
2	9.747998668274988	3.0085024966678104
3	3.2097624248213883	1.4859324355798007
4	1.5188746466625511	0.9375336050221992
5	0.8959029536195309	0.6912511902336438
6	0.5998730171862516	0.5554123050186631
7	0.4507558973067859	0.4869051597196342
8	0.34870340995884913	0.43047813819343556
9	0.2672825638385675	0.37120862404147265
>10	2.283163821440931	7.662975007499899
>50	0.8035436116041079	9.165127462556267
>100	1.886677808105988	60.8695249245757
>500	0.009301947963014874	0.8917387276314535
>1k	0.0036398926811797333	0.9316808339186167
>5k	0.0	0.0
>10k+	2.022162600655407E-4	0.4792116611343469
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15357	0.4744736746977343	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3944	0.1218548006126108	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.268874285949097E-5	0.0	0.0	0.06151442901108217	0.0
2	9.268874285949097E-5	0.0	0.0	0.19291617013822054	0.0
3	9.268874285949097E-5	0.0	0.0	0.27899311600706783	0.0
4	9.268874285949097E-5	0.0	0.0	0.4210231663154278	0.0
5	9.268874285949097E-5	0.0	0.0	0.7217672406468562	0.0
6	9.268874285949097E-5	0.0	0.0	0.9484839056811711	0.0
7	9.268874285949097E-5	0.0	0.0	1.1103493469614623	0.0
8	9.268874285949097E-5	0.0	0.0	1.4363974480935315	0.0
9	9.268874285949097E-5	0.0	0.0	1.5344312417912531	0.0
10	9.268874285949097E-5	0.0	0.0	1.7336193501962993	0.0
11	9.268874285949097E-5	0.0	0.0	2.075455433862102	0.0
12	9.268874285949097E-5	0.0	0.0	2.3176820152015716	0.0
13	9.268874285949097E-5	0.0	0.0	2.402677592403725	0.0
14	9.268874285949097E-5	0.0	0.0	2.4378684184427115	0.0
15	9.268874285949097E-5	0.0	0.0	2.5004642161204877	0.0
16	9.268874285949097E-5	0.0	0.0	2.632452985952403	0.0
17	9.268874285949097E-5	0.0	0.0	2.8222795313286406	0.0
18	9.268874285949097E-5	0.0	0.0	3.025236981943306	0.0
19	9.268874285949097E-5	0.0	0.0	3.174929301661384	0.0
20	9.268874285949097E-5	0.0	0.0	3.32496148010328	0.0
21	9.268874285949097E-5	0.0	0.0	3.500513959079156	0.0
22	9.268874285949097E-5	0.0	0.0	3.710083206684465	0.0
23	9.268874285949097E-5	0.0	0.0	3.919096321832617	0.0
24	9.268874285949097E-5	0.0	0.0	4.0723726062745955	0.0
25	9.268874285949097E-5	0.0	0.0	4.209582841954262	0.0
26	9.268874285949097E-5	0.0	0.0	4.34101547932902	0.0
27	9.268874285949097E-5	0.0	0.0	4.4831382183802395	0.0
28	9.268874285949097E-5	0.0	0.0	4.637217805260334	0.0
29	9.268874285949097E-5	0.0	0.0	4.822533498484075	0.0
30	9.268874285949097E-5	0.0	0.0	5.024440476679667	0.0
31	9.268874285949097E-5	0.0	0.0	5.208149565027178	0.0
32	9.268874285949097E-5	0.0	0.0	5.37335180105041	0.0
33	9.268874285949097E-5	0.0	0.0	5.544980456578568	0.0
34	1.235849904793213E-4	0.0	0.0	5.733694737040492	0.0
35	1.235849904793213E-4	0.0	0.0	5.944129079579156	0.0
36	1.235849904793213E-4	0.0	0.0	6.137168834707856	0.0
37	1.235849904793213E-4	0.0	0.0	6.335306470693828	0.0
38	1.235849904793213E-4	0.0	0.0	6.537831373841816	0.0
39	1.5448123809915163E-4	0.0	0.0	6.79189121801968	0.0
40	1.8537748571898193E-4	0.0	0.0	7.036342329187778	0.0
41	2.471699809586426E-4	0.0	0.0	7.266333996469795	0.0
42	2.780662285784729E-4	0.0	0.0	7.491598537865977	0.0
43	2.780662285784729E-4	0.0	0.0	7.719303882824127	0.0
44	2.780662285784729E-4	0.0	0.0	7.94277644185836	0.0
45	2.780662285784729E-4	0.0	0.0	8.16856621946408	0.0
46	2.780662285784729E-4	0.0	0.0	8.414654831756028	0.0
47	2.780662285784729E-4	0.0	0.0	8.664852644981414	0.0
48	2.780662285784729E-4	0.0	0.0	8.921415085216486	0.0
49	2.780662285784729E-4	0.0	0.0	9.187493569718464	0.0
50	2.780662285784729E-4	0.0	0.0	9.45953503001107	0.0
51	3.0896247619830326E-4	0.0	0.0	9.72552082577019	0.0
52	3.0896247619830326E-4	0.0	0.0	10.020610886787189	0.0
53	3.0896247619830326E-4	0.0	0.0	10.305505186089643	0.0
54	3.0896247619830326E-4	0.0	0.0	10.592932977696925	0.0
55	3.0896247619830326E-4	0.0	0.0	10.87603529463743	0.0
56	3.0896247619830326E-4	0.0	0.0	11.171156251902051	0.0
57	3.0896247619830326E-4	0.0	0.0	11.46260055569991	0.0
58	3.0896247619830326E-4	0.0	0.0	11.744034475268943	0.0
59	3.0896247619830326E-4	0.0	0.0	12.046230673238504	0.0
60	3.0896247619830326E-4	0.0	0.0	12.347963427493767	0.0
61	3.0896247619830326E-4	0.0	0.0	12.66075703839693	0.0
62	3.0896247619830326E-4	0.0	0.0	12.99925632731979	0.0
63	3.0896247619830326E-4	0.0	0.0	13.360649735728947	0.0
64	3.0896247619830326E-4	0.0	0.0	13.698747373432749	0.0
65	3.0896247619830326E-4	0.0	0.0	14.025073540793397	0.0
66	3.398587238181336E-4	0.0	0.0	14.332491204610708	0.0
67	3.398587238181336E-4	0.0	0.0	14.653008877418829	0.0
68	3.398587238181336E-4	0.0	0.0	14.988820192798764	0.0
69	3.7075497143796386E-4	0.0	0.0	15.374528948084727	0.0
70	3.7075497143796386E-4	0.0	0.0	15.713677058207603	0.0
71	3.7075497143796386E-4	0.0	0.0	16.06366975124504	0.0
72	4.016512190577942E-4	0.0	0.0	16.41570159662539	0.0
73	4.325474666776245E-4	0.0	0.0	16.755653009186382	0.0
74	4.325474666776245E-4	0.0	0.0	17.108828015728662	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	55	0.0	72.72727	1
CGAGGGA	2865	0.0	69.38918	4
CGTTAGG	530	0.0	68.679245	2
CGATAGG	530	0.0	68.679245	2
CAACGCG	105	0.0	68.571434	1
CGTTTTT	8115	0.0	68.02218	1
GTTTGCG	265	0.0	67.92452	1
AGGGATT	7745	0.0	67.34667	6
ACGGGAT	2035	0.0	67.22359	5
TAGGGAC	4375	0.0	66.10286	5
TACGGGA	1780	0.0	65.8427	4
TTAGGGA	5175	0.0	64.77295	4
AGCGGGA	2480	0.0	63.870968	4
CGGGATA	1005	0.0	63.68159	6
CGGGACG	145	0.0	63.44828	6
CGTAGGG	1690	0.0	63.431953	3
ACGTAGG	790	0.0	63.29114	2
GGGACCG	1645	0.0	63.22189	7
AGGGATG	6115	0.0	62.992638	6
TAAGGGA	5490	0.0	62.36794	4
>>END_MODULE