FastQCFastQC Report
Thu 26 May 2016
SRR1547796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547796_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2135665
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110890.5192293735206599No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA78190.36611547222996116No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA71750.33596093020206824No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA62960.2948027897633758No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA44790.209723903327535No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA41550.19455298466753915No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37740.17671310809513663No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA31940.1495552907408231No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29580.138504868506999No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA25730.12047769664249777No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25050.11729367667681963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG251.2851851E-680.01
TGTCGCG850.075.294121
TTACGGG5650.075.044243
GCGTAAG1600.075.01
TACGGGA7950.072.452834
AATACGG2000.072.02
GCATGCG850.070.588241
CGAAACG6450.070.0775280
TCGCGTG403.7161954E-970.01
ATAGCGG4350.069.8850562
ACGGGAT5800.069.655175
ACGCGAG2500.068.81
TAACGCG700.068.571431
GCGACGG1000.068.02
CGTAAGG3600.067.777782
CGTTTTT66100.067.715581
TAAGGGA25900.067.490354
GTGTACG1850.067.027021
GATCGAC1200.066.6666648
CGGGACG1200.066.6666646