##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547796_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2135665
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864578948477405	31.0	31.0	33.0	30.0	34.0
2	32.31563798629467	33.0	31.0	34.0	31.0	34.0
3	32.45275406021075	34.0	31.0	34.0	31.0	34.0
4	36.02721962480071	37.0	35.0	37.0	35.0	37.0
5	35.9745390779921	37.0	35.0	37.0	35.0	37.0
6	36.02939037723613	37.0	35.0	37.0	35.0	37.0
7	36.24238539284017	37.0	35.0	37.0	35.0	37.0
8	36.19340205509759	37.0	36.0	37.0	35.0	37.0
9	38.01459498563679	39.0	39.0	39.0	35.0	39.0
10-11	37.60573521596318	39.0	37.5	39.0	35.0	39.0
12-13	36.59407982993588	39.0	35.0	39.0	33.0	39.0
14-15	37.64090505767524	40.0	35.0	41.0	33.0	41.0
16-17	37.82363409055259	40.0	35.0	41.0	34.0	41.0
18-19	37.70942914736159	39.0	36.0	41.0	34.0	41.0
20-21	37.49160308381698	39.0	35.0	41.0	33.5	41.0
22-23	37.29998829404424	39.0	35.0	41.0	33.0	41.0
24-25	37.23060217777601	39.0	35.0	41.0	33.0	41.0
26-27	37.185965963763046	39.0	35.0	41.0	33.0	41.0
28-29	37.19819025924009	39.0	35.0	41.0	33.0	41.0
30-31	37.04093877082782	39.0	35.0	41.0	33.0	41.0
32-33	36.70146394682686	38.5	35.0	41.0	32.0	41.0
34-35	36.45494564924742	38.0	35.0	41.0	31.0	41.0
36-37	36.25327778467128	38.0	35.0	41.0	31.0	41.0
38-39	36.19282752678908	38.0	35.0	41.0	31.0	41.0
40-41	36.04131546848406	38.0	35.0	41.0	30.0	41.0
42-43	36.03455012841434	38.0	35.0	41.0	30.0	41.0
44-45	35.917691679172535	38.0	35.0	41.0	30.0	41.0
46-47	35.83565212708922	38.0	35.0	40.5	30.0	41.0
48-49	35.74681539473653	37.5	35.0	40.0	30.0	41.0
50-51	35.60313672790443	37.0	35.0	40.0	29.5	41.0
52-53	35.42576246742817	37.0	35.0	40.0	29.0	41.0
54-55	35.25981064445969	36.5	35.0	40.0	29.0	41.0
56-57	34.98368915536847	36.0	34.0	40.0	28.5	41.0
58-59	34.695655451580656	36.0	34.0	40.0	27.0	41.0
60-61	34.40017137519227	35.0	34.0	39.0	26.5	41.0
62-63	33.95077271013946	35.0	33.5	39.0	26.0	41.0
64-65	33.720743421838165	35.0	33.0	38.5	26.0	40.0
66-67	33.47691983527379	35.0	33.0	38.0	25.0	40.0
68-69	33.13400907913928	35.0	33.0	37.0	24.5	40.0
70-71	32.89531457414904	35.0	33.0	36.5	24.0	39.0
72-73	32.65372799572967	35.0	33.0	36.0	24.0	39.0
74-75	32.36807949748673	35.0	33.0	36.0	23.5	38.0
76-77	30.31770315100917	33.0	29.5	34.5	19.5	36.0
78-79	31.353945024149386	34.0	31.5	35.0	20.0	37.0
80-81	31.72153778799578	35.0	32.0	35.0	21.5	36.5
82-83	31.61227814287353	35.0	32.0	35.0	20.0	36.0
84-85	31.399583267975082	35.0	32.0	35.0	20.0	36.0
86	30.981339301809975	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	23.0
10	39.0
11	35.0
12	65.0
13	118.0
14	387.0
15	1059.0
16	2413.0
17	5356.0
18	9685.0
19	14426.0
20	17340.0
21	18131.0
22	16178.0
23	15131.0
24	15800.0
25	17103.0
26	19765.0
27	23808.0
28	29123.0
29	36262.0
30	45799.0
31	57795.0
32	72687.0
33	100067.0
34	275202.0
35	250636.0
36	161709.0
37	295775.0
38	416138.0
39	216874.0
40	732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.008889502801235	26.061203419075557	29.07515925952806	13.854747818595145
2	30.52866437386013	27.325680759857	28.368213179501467	13.7774416867814
3	30.06913537469594	24.74264456270061	31.513416195892148	13.674803866711304
4	27.143957502698225	27.684632187164187	30.618051052014245	14.553359258123347
5	25.26805468086053	32.065750012291254	28.553260928094996	14.112934378753222
6	22.383941301655454	41.58283251352623	25.519779553441204	10.513446631377112
7	85.6911079218885	5.650792610264251	6.76421629796808	1.8938831698791712
8	86.35675539000732	5.22052850049048	6.522933137921911	1.8997829715802808
9	80.30159224410195	9.043272236048256	7.860408818798828	2.7947267010509607
10-11	43.82117513748645	28.71639512751298	17.90634298918604	9.556086745814534
12-13	32.439872358258434	29.473302226706906	23.465337494410406	14.621487920624254
14-15	15.102532466468292	35.367812835814604	34.536338798453876	14.993315899263226
16-17	18.16736239063711	25.90090206095057	34.33825061514798	21.593484933264346
18-19	23.777277803400814	29.45321480662932	29.36506895978536	17.40443843018451
20-21	26.55491380904777	29.19671390410013	27.51452123811553	16.733851048736575
22-23	21.128898961213487	28.023870784977984	28.234460929031474	22.61276932477706
24-25	18.700334556215513	28.349670945583693	34.6430034673041	18.3069910308967
26-27	16.58787778045714	37.92881842423788	28.237293770324467	17.24601002498051
28-29	15.335644869396653	31.361426066354042	36.19963805184802	17.10329101240129
30-31	23.348933470371055	31.639138160713408	27.857388682213735	17.1545396867018
32-33	26.723994633989882	31.76521130420735	25.47056771544226	16.0402263463605
34-35	19.371156056778567	31.201148120140566	28.673504505622372	20.754191317458496
36-37	22.966968134047242	32.961161979992184	27.946892419925412	16.12497746603517
38-39	19.31964985145142	34.31444070113992	26.808441398814892	19.557468048593762
40-41	20.10537701371704	29.38548414662412	28.076758293084353	22.43238054657449
42-43	21.045107729910825	28.3271018628858	27.146766932079704	23.481023475123674
44-45	18.025486206872333	36.31618254735645	25.938361119370313	19.71997012640091
46-47	20.51611558928952	33.77720756766628	26.11739200670517	19.589284836339033
48-49	20.649282541971704	28.566067243692245	30.933011497589746	19.851638716746304
50-51	17.96892302865852	34.01572812215399	27.611680670891737	20.403668178295753
52-53	21.8664209976752	28.27629801490402	29.68283883474234	20.17444215267844
54-55	21.854785277653566	29.344934715884747	30.061105089047206	18.739174917414484
56-57	23.488819641657283	27.371989520828404	27.735670154261083	21.403520683253223
58-59	23.93104723821386	29.528905516548708	27.04637665551479	19.49367058972264
60-61	24.464300346730408	31.285758768346163	25.92824717359698	18.321693711326446
62-63	23.424811475582548	27.296017867970868	31.07470038606242	18.204470270384164
64-65	18.885850543039286	33.32968887910791	29.819938988558597	17.9645215892942
66-67	18.76296141951102	30.52791519269174	24.784083646077452	25.92503974171979
68-69	17.25930799071952	35.673291457227606	23.8007833625592	23.266617189493672
70-71	19.770680326736638	31.773475708971212	28.70681965570443	19.749024308587725
72-73	22.705035668047188	33.851821329656104	24.71862394148895	18.724519060807758
74-75	21.06109806547375	34.85471738311018	23.33570574036658	20.748478811049488
76-77	26.587456366049917	29.727485349996368	24.91600976744948	18.769048516504228
78-79	18.5183537680301	32.14949441977089	29.506195962381742	19.82595584981727
80-81	15.737861509178641	30.39392882310662	30.48582057579255	23.38238909192219
82-83	18.601161699049243	29.35987151542962	30.437521802342598	21.601444983178542
84-85	16.50558491149127	28.76556014168889	28.43959609770259	26.289258849117253
86	17.883703670753608	28.430442040301262	30.964125927989645	22.72172836095549
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2351.0
1	2504.0
2	5325.0
3	5738.0
4	3707.0
5	4391.5
6	5296.0
7	5740.0
8	5709.5
9	5717.0
10	5494.5
11	5169.5
12	4992.0
13	4953.0
14	5027.0
15	5007.5
16	5250.0
17	5910.0
18	6253.0
19	6281.5
20	6812.5
21	7260.0
22	7067.0
23	7252.5
24	9142.5
25	11448.0
26	21185.5
27	30307.5
28	39883.0
29	49280.0
30	50515.0
31	51739.5
32	47308.5
33	42467.5
34	45904.5
35	52607.5
36	55453.0
37	58094.0
38	61151.0
39	65037.5
40	74724.0
41	90521.0
42	105279.0
43	110456.0
44	114307.5
45	115922.5
46	119814.5
47	130067.5
48	121657.0
49	93478.5
50	77835.0
51	68830.5
52	55911.5
53	52084.5
54	48825.0
55	46088.5
56	43043.5
57	39388.0
58	36239.0
59	26897.0
60	19239.0
61	18407.5
62	18706.5
63	15748.5
64	13125.0
65	9721.5
66	4591.0
67	2547.0
68	1925.0
69	1254.5
70	975.0
71	1061.0
72	826.0
73	451.0
74	294.5
75	174.5
76	51.5
77	36.0
78	88.5
79	137.0
80	83.0
81	20.5
82	9.0
83	4.5
84	5.5
85	5.5
86	3.0
87	2.0
88	2.0
89	1.5
90	1.5
91	1.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2135665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.638611284002899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.64929760999568	10.99944420430595
2	9.12063362150807	2.487855532551124
3	2.6393508624119417	1.0799124136360287
4	1.1698177155028284	0.6381875637953347
5	0.6576201029638918	0.44845124784352414
6	0.4491001390681682	0.36750613346054334
7	0.3339450448143517	0.318818265950931
8	0.26515422341931644	0.2893068306822172
9	0.20866598173352952	0.25613227917526127
>10	1.7829858152520144	5.273261797314339
>50	0.6998659299047164	7.102948141720318
>100	1.9623894875405505	62.65888323651123
>500	0.051496307439862964	4.304059980731076
>1k	0.008294707238635644	2.2489903603180497
>5k	0.0010368384048294555	1.003542185847822
>10k+	3.4561280160981853E-4	0.522699826156247
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11089	0.5192293735206599	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	7819	0.36611547222996116	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	7175	0.33596093020206824	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6296	0.2948027897633758	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	4479	0.209723903327535	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4155	0.19455298466753915	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3774	0.17671310809513663	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3194	0.1495552907408231	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2958	0.138504868506999	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2573	0.12047769664249777	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2505	0.11729367667681963	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8729529209871398E-4	0.0	0.0	0.02668957912406674	0.0
2	1.8729529209871398E-4	0.0	0.0	0.11752779579194303	0.0
3	1.8729529209871398E-4	0.0	0.0	0.18584375358494895	0.0
4	1.8729529209871398E-4	0.0	0.0	0.294194080064055	0.0
5	1.8729529209871398E-4	0.0	0.0	0.5012958493022079	0.0
6	1.8729529209871398E-4	0.0	0.0	0.6685505451463596	0.0
7	1.8729529209871398E-4	0.0	0.0	0.7685662311270728	0.0
8	1.8729529209871398E-4	0.0	0.0	0.9512727885693684	0.0
9	1.8729529209871398E-4	0.0	0.0	1.017903088733486	0.0
10	1.8729529209871398E-4	0.0	0.0	1.1454980064757347	0.0
11	1.8729529209871398E-4	0.0	0.0	1.382286079511534	0.0
12	1.8729529209871398E-4	0.0	0.0	1.5508986662234012	0.0
13	1.8729529209871398E-4	0.0	0.0	1.6180440284407902	0.0
14	1.8729529209871398E-4	0.0	0.0	1.6461383222555972	0.0
15	1.8729529209871398E-4	0.0	0.0	1.6870155197561416	0.0
16	1.8729529209871398E-4	0.0	0.0	1.7785092699463634	0.0
17	1.8729529209871398E-4	0.0	0.0	1.879648727679669	0.0
18	1.8729529209871398E-4	0.0	0.0	2.0028890298806226	0.0
19	1.8729529209871398E-4	0.0	0.0	2.079305509056898	0.0
20	1.8729529209871398E-4	0.0	0.0	2.1642439240236646	0.0
21	1.8729529209871398E-4	0.0	0.0	2.265617500872094	0.0
22	1.8729529209871398E-4	0.0	0.0	2.3703623929782993	0.0
23	1.8729529209871398E-4	0.0	0.0	2.481568972661911	0.0
24	1.8729529209871398E-4	0.0	0.0	2.5638852535392957	0.0
25	1.8729529209871398E-4	0.0	0.0	2.63318451161582	0.0
26	1.8729529209871398E-4	0.0	0.0	2.70234329822327	0.0
27	1.8729529209871398E-4	0.0	0.0	2.7762312909562126	0.0
28	1.8729529209871398E-4	0.0	0.0	2.8617784156223003	0.0
29	1.8729529209871398E-4	0.0	0.0	2.9514460367145596	0.0
30	1.8729529209871398E-4	0.0	0.0	3.052725965916939	0.0
31	1.8729529209871398E-4	0.0	0.0	3.158500982129688	0.0
32	1.8729529209871398E-4	0.0	0.0	3.252242275825094	0.0
33	1.8729529209871398E-4	0.0	0.0	3.3455153312902537	0.0
34	1.8729529209871398E-4	0.0	0.0	3.4432834737657827	0.0
35	1.8729529209871398E-4	0.0	0.0	3.5651658850990207	0.0
36	1.8729529209871398E-4	0.0	0.0	3.6678973528151655	0.0
37	2.3411911512339248E-4	0.0	0.0	3.7757326172409997	0.0
38	2.3411911512339248E-4	0.0	0.0	3.8886248545534996	0.0
39	2.3411911512339248E-4	0.0	0.0	4.02984550479593	0.0
40	2.3411911512339248E-4	0.0	0.0	4.171440745622558	0.0
41	2.3411911512339248E-4	0.0	0.0	4.303905340959373	0.0
42	2.3411911512339248E-4	0.0	0.0	4.450323435557543	0.0
43	2.3411911512339248E-4	0.0	0.0	4.563262496693068	0.0
44	2.3411911512339248E-4	0.0	0.0	4.690951062081366	0.0
45	2.8094293814807095E-4	0.0	0.0	4.817890446301269	0.0
46	2.8094293814807095E-4	0.0	0.0	4.968101270564437	0.0
47	2.8094293814807095E-4	0.0	0.0	5.137931276674947	0.0
48	2.8094293814807095E-4	0.0	0.0	5.271285524649231	0.0
49	2.8094293814807095E-4	0.0	0.0	5.418031386008574	0.0
50	2.8094293814807095E-4	0.0	0.0	5.564496304429768	0.0
51	2.8094293814807095E-4	0.0	0.0	5.716111843383676	0.0
52	2.8094293814807095E-4	0.0	0.0	5.896711328789862	0.0
53	2.8094293814807095E-4	0.0	0.0	6.052494187992967	0.0
54	2.8094293814807095E-4	0.0	0.0	6.222136898811377	0.0
55	2.8094293814807095E-4	0.0	0.0	6.38700357968127	0.0
56	2.8094293814807095E-4	0.0	0.0	6.560251724872581	0.0
57	2.8094293814807095E-4	0.0	0.0	6.733827636825064	0.0
58	2.8094293814807095E-4	0.0	0.0	6.898834789164031	0.0
59	2.8094293814807095E-4	0.0	0.0	7.07817003134855	0.0
60	2.8094293814807095E-4	0.0	0.0	7.259893288507326	0.0
61	2.8094293814807095E-4	0.0	0.0	7.45177731526246	0.0
62	2.8094293814807095E-4	0.0	0.0	7.667260548822029	0.0
63	2.8094293814807095E-4	0.0	0.0	7.88934594142808	0.0
64	2.8094293814807095E-4	0.0	0.0	8.091437561602593	0.0
65	2.8094293814807095E-4	0.0	0.0	8.279856625453899	0.0
66	3.2776676117274946E-4	0.0	0.0	8.466870974614464	0.0
67	3.2776676117274946E-4	0.0	0.0	8.660955721051756	0.0
68	3.2776676117274946E-4	0.0	0.0	8.891235282687125	0.0
69	3.2776676117274946E-4	0.0	0.0	9.139635663833046	0.0
70	3.7459058419742796E-4	0.0	0.0	9.340182097847743	0.0
71	4.2141440722210646E-4	0.0	0.0	9.544755380642563	0.0
72	4.2141440722210646E-4	0.0	0.0	9.764920996504602	0.0
73	4.2141440722210646E-4	0.0	0.0	9.979608225072752	0.0
74	4.2141440722210646E-4	0.0	0.0	10.224356348022747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	25	1.2851851E-6	80.0	1
TGTCGCG	85	0.0	75.29412	1
TTACGGG	565	0.0	75.04424	3
GCGTAAG	160	0.0	75.0	1
TACGGGA	795	0.0	72.45283	4
AATACGG	200	0.0	72.0	2
GCATGCG	85	0.0	70.58824	1
CGAAACG	645	0.0	70.07752	80
TCGCGTG	40	3.7161954E-9	70.0	1
ATAGCGG	435	0.0	69.885056	2
ACGGGAT	580	0.0	69.65517	5
ACGCGAG	250	0.0	68.8	1
TAACGCG	70	0.0	68.57143	1
GCGACGG	100	0.0	68.0	2
CGTAAGG	360	0.0	67.77778	2
CGTTTTT	6610	0.0	67.71558	1
TAAGGGA	2590	0.0	67.49035	4
GTGTACG	185	0.0	67.02702	1
GATCGAC	120	0.0	66.666664	8
CGGGACG	120	0.0	66.666664	6
>>END_MODULE