FastQCFastQC Report
Thu 26 May 2016
SRR1547795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547795_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3643120
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210670.5782680779112409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG1100.072.727272
ATTGCGG6750.069.925932
CGTTTTT108600.069.208111
GGCGATA6600.068.484858
CGTTAGG6550.067.7862552
AGGGATT74450.066.0846256
TAAGGGA53550.065.9570544
TCGATAG850.065.8823551
TACGGGA23150.064.96764
TAAGCGG9650.064.248712
CGAGGGA24000.064.1666644
ATAGGGA54650.063.0924074
TCGTTAG2750.062.5454561
TAGGGAG45700.062.494535
ATACCGG1500.061.333332
TTGCGGG29950.061.302173
GCGAGGG32850.061.1263353
TGCGGGC10100.060.9900974
TGGGCGA8600.060.930236
TAGGGAT66650.060.9152265
Produced by FastQC (version 0.11.5)