FastQCFastQC Report
Thu 26 May 2016
SRR1547794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547794_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2856331
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120280.42109965546710093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG204.0919418E-580.03
GCGCGAC1400.074.285719
CGTTAGG4700.072.340422
GTTAGCG1350.071.111111
TATAGCG1650.070.303031
CGTTTTT77800.068.791771
TAGCGCG1400.068.571431
TAGGGTA28250.067.5398255
TAACGCG900.066.666671
TTCGTAG2700.066.6666641
TACGGGT7450.066.040274
CGGGATC6200.065.806456
TACGCGG1100.065.4545442
GTACGGG8650.064.277463
CGAGGGT9100.064.175824
ACGGGTA8000.063.05
AGGGTAC21150.062.9787256
CGAGGGA16200.062.9629674
GCGAGGG22300.062.6008953
CGGGTGC7300.061.9178056
Produced by FastQC (version 0.11.5)