##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547794_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2856331
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729334240324388	31.0	31.0	33.0	30.0	34.0
2	32.16926364626509	33.0	31.0	34.0	30.0	34.0
3	32.344559156484316	34.0	31.0	34.0	30.0	34.0
4	35.94581020196889	37.0	35.0	37.0	35.0	37.0
5	35.89267910476762	37.0	35.0	37.0	35.0	37.0
6	35.94490239401526	37.0	35.0	37.0	35.0	37.0
7	36.211231821522084	37.0	35.0	37.0	35.0	37.0
8	36.15443973405043	37.0	35.0	37.0	35.0	37.0
9	38.00433388147242	39.0	39.0	39.0	35.0	39.0
10-11	37.43320889630789	39.0	37.0	39.0	35.0	39.0
12-13	36.879064786259015	39.0	35.0	39.0	33.0	39.0
14-15	37.95124812215391	40.0	36.0	41.0	33.0	41.0
16-17	38.100371070439664	40.0	36.0	41.0	33.5	41.0
18-19	37.99461582008527	40.0	36.0	41.0	33.5	41.0
20-21	37.80176247080608	40.0	35.0	41.0	33.0	41.0
22-23	37.619995896834084	39.5	35.0	41.0	33.0	41.0
24-25	37.54829972436668	39.0	35.0	41.0	33.0	41.0
26-27	37.53811235462557	40.0	35.0	41.0	33.0	41.0
28-29	37.51116642293908	40.0	35.0	41.0	33.0	41.0
30-31	37.34237436067458	40.0	35.0	41.0	33.0	41.0
32-33	37.0675231617064	40.0	35.0	41.0	32.0	41.0
34-35	36.8222100309803	39.0	35.0	41.0	31.0	41.0
36-37	36.62179733371238	39.0	35.0	41.0	31.0	41.0
38-39	36.517820588720284	39.0	35.0	41.0	30.0	41.0
40-41	36.359942002520015	39.0	35.0	41.0	30.0	41.0
42-43	36.33215180593566	39.0	35.0	41.0	30.0	41.0
44-45	36.23437689819562	39.0	35.0	41.0	30.0	41.0
46-47	36.16049347922212	39.0	35.0	41.0	30.0	41.0
48-49	36.042546189499745	38.0	35.0	41.0	30.0	41.0
50-51	35.85607637910313	38.0	35.0	40.0	29.0	41.0
52-53	35.6786291574751	38.0	35.0	40.0	29.0	41.0
54-55	35.49911179761729	37.5	35.0	40.0	28.5	41.0
56-57	35.21860876768133	37.0	34.5	40.0	27.5	41.0
58-59	34.929117983875116	37.0	34.0	40.0	27.0	41.0
60-61	34.62839233268133	36.0	34.0	39.5	26.5	41.0
62-63	34.25272351138576	36.0	33.5	39.0	26.0	41.0
64-65	33.95930303595767	35.0	33.0	39.0	26.0	40.0
66-67	33.672499790815564	35.0	33.0	38.5	25.0	40.0
68-69	33.236213169972245	35.0	33.0	37.5	24.0	40.0
70-71	32.9837215994925	35.0	33.0	37.0	24.0	39.5
72-73	32.70551767284674	35.0	33.0	36.5	24.0	39.0
74-75	32.40705523974637	35.0	32.5	36.0	23.5	39.0
76-77	30.319593737560528	33.0	29.5	34.5	19.5	36.0
78-79	31.47260769147553	34.0	31.5	35.0	20.0	37.0
80-81	31.6908654844274	35.0	32.0	35.0	20.0	37.0
82-83	31.519016528546587	35.0	32.0	35.0	20.0	36.0
84-85	31.305697413920164	34.5	32.0	35.0	20.0	36.0
86	30.864516752435204	34.0	31.0	35.0	19.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	18.0
10	16.0
11	33.0
12	76.0
13	177.0
14	452.0
15	1342.0
16	3323.0
17	7097.0
18	13393.0
19	19650.0
20	22044.0
21	22061.0
22	19822.0
23	19195.0
24	20779.0
25	23324.0
26	27453.0
27	33520.0
28	41065.0
29	50914.0
30	63071.0
31	77045.0
32	96023.0
33	126932.0
34	280874.0
35	266014.0
36	251805.0
37	433166.0
38	625768.0
39	308470.0
40	1403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.038106928083614	23.26232498964581	29.9112742885891	12.788293793681474
2	31.53451053116743	25.860938385642278	29.711087405486268	12.89346367770402
3	30.057265772069137	25.453842709405876	31.468411749198534	13.020479769326453
4	27.199613770252817	27.627995494919883	30.7969909649827	14.375399769844602
5	24.695352184323173	32.03998416149949	28.91426098725953	14.350402666917805
6	22.55606930709361	41.417748853336676	25.892657398599813	10.133524440969902
7	87.92202304284763	4.050966082012204	6.330603841081444	1.6964070340587278
8	88.23560714777105	3.9446058597550495	6.002455597758103	1.8173313947158085
9	83.31394365709016	6.006656791527313	8.085897607805258	2.5935019435772677
10-11	35.90777819517416	27.814563508220864	24.270751534048397	12.00690676255658
12-13	29.0479989889127	27.397174907249894	26.293556314026628	17.261269789810775
14-15	17.95031808288325	32.1973713830785	33.0688565155789	16.783454018459345
16-17	22.49574716655738	27.710233862952162	31.34323367985013	18.450785290640333
18-19	24.643484946247476	28.937962722107486	28.977296398771713	17.441255932873325
20-21	26.55392529787339	28.988324532415888	28.368193322132484	16.08955684757824
22-23	22.161927311645606	25.68826932172777	31.379906600460522	20.769896766166106
24-25	19.839787475611196	28.92516658608544	32.81564706611383	18.419398872189532
26-27	18.708948647758262	34.59824859233751	28.089426610571394	18.603376149332835
28-29	17.522479012411377	30.331411170484092	33.58625103323109	18.559858783873437
30-31	21.968479843547545	29.82353935870878	29.867966982818167	18.34001381492551
32-33	24.33863582336921	29.535214931322734	28.061873781434993	18.06427546387306
34-35	19.981350200659517	29.715148559463174	29.559109220885116	20.744392018992198
36-37	22.56037553070705	30.182041227014654	28.771175329469866	18.486407912808424
38-39	20.320404042808764	31.21733790656615	28.502018848655847	19.96023920196924
40-41	20.739035496936452	28.198902718207382	29.214996441238778	21.847065343617388
42-43	21.18119363617172	27.505110577170505	28.842315543961817	22.47138024269596
44-45	20.008727980055532	31.57795787673067	27.981893555053666	20.431420588160126
46-47	21.00546120180049	30.864070025497746	27.760823237923056	20.36964553477871
48-49	21.443208087578085	28.482780882187676	30.01376591158378	20.060245118650464
50-51	19.4383984209113	31.848147151012963	28.46347289582335	20.249981532252388
52-53	21.766454938170682	28.27765759640602	29.205036111010944	20.750851354412355
54-55	21.88946239073833	28.49738003053568	29.687140601001776	19.92601697772422
56-57	22.00028638137527	27.373473172401937	28.91613401948164	21.710106426741156
58-59	22.634369055967255	28.905578520136498	28.294269816768434	20.165782607127817
60-61	22.949913717982966	30.100503057943918	27.29225009286389	19.657333131209235
62-63	21.98703511602822	28.155035253267215	30.16154290241572	19.696386728288843
64-65	20.320053943327995	30.660609712249737	29.622004592604988	19.39733175181728
66-67	19.698557345069602	30.29645373732946	26.406218326937598	23.59877059066334
68-69	18.604058843320328	32.35881975863442	26.18381763178007	22.853303766265185
70-71	19.776506994462476	30.436983668909523	28.79102597002938	20.99548336659862
72-73	22.143091959580318	30.287508695595854	27.610683075595933	19.958716269227903
74-75	21.036497520770528	31.5378714861828	26.17898625894548	21.246644734101196
76-77	23.059494855463182	29.381906368694665	27.25611982644869	20.30247894939347
78-79	19.202833985276914	30.285110514152596	29.23099598750985	21.281059513060637
80-81	17.109869269352888	29.880045414904643	30.00074221089923	23.009343104843243
82-83	19.28748803972649	28.533562811872994	30.482969235708328	21.69597991269219
84-85	17.78636649603985	28.627512007536943	29.331334498697803	24.254786997725404
86	18.554222182233083	29.387630495205215	30.16534148178205	21.89280584077966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1711.0
1	2084.0
2	4716.0
3	4757.5
4	2953.5
5	3955.0
6	4974.0
7	5405.0
8	5533.0
9	5727.0
10	5607.0
11	5431.0
12	5445.5
13	5565.5
14	5681.0
15	5973.0
16	6277.5
17	6601.0
18	7159.0
19	8374.5
20	9370.5
21	9398.0
22	9538.5
23	11363.5
24	13314.5
25	17113.0
26	23321.5
27	28492.0
28	34350.5
29	37715.0
30	43089.0
31	51441.0
32	59363.5
33	67312.0
34	69839.5
35	73738.5
36	78114.0
37	83277.0
38	92306.0
39	99586.5
40	111571.0
41	128952.5
42	143775.5
43	149787.0
44	152972.5
45	153373.5
46	155650.5
47	162100.0
48	157337.5
49	139392.5
50	127598.0
51	112066.5
52	91652.5
53	83114.0
54	75114.0
55	64834.5
56	57411.5
57	55924.0
58	48358.0
59	33804.5
60	25694.5
61	22741.0
62	19202.0
63	14097.5
64	10701.0
65	8070.5
66	4499.0
67	3270.0
68	2861.0
69	2117.5
70	1353.5
71	1212.0
72	960.5
73	504.5
74	238.0
75	126.0
76	66.5
77	47.0
78	39.0
79	41.0
80	53.0
81	46.5
82	19.5
83	10.0
84	8.0
85	6.5
86	6.0
87	4.0
88	2.5
89	2.0
90	0.5
91	0.5
92	0.5
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2856331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.410530141032035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.36081046734809	12.384026918648305
2	8.64201765872734	2.663561472182976
3	2.6386889595791496	1.2199078723320884
4	1.2013536124890858	0.7405398422120311
5	0.7053905586885855	0.5435221232934936
6	0.49463286566493103	0.4573532811044681
7	0.3376891492058882	0.36427781684957633
8	0.26740162900381353	0.3296640690819468
9	0.2216415167004191	0.30740519462542776
>10	2.2655703777349845	8.112349108390493
>50	0.9184211995503616	10.374860210498591
>100	1.9344845251039606	60.569390300451865
>500	0.009151907848817406	0.9263228175081099
>1k	0.0025167746584247867	0.5827244673019386
>5k	0.0	0.0
>10k+	2.2879769622043513E-4	0.4240945055187099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12028	0.42109965546710093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03374958994598315	0.0
2	0.0	0.0	0.0	0.1287665890262718	0.0
3	0.0	0.0	0.0	0.1956355898528567	0.0
4	0.0	0.0	0.0	0.294013543948513	0.0
5	0.0	0.0	0.0	0.4892640243725255	0.0
6	0.0	0.0	0.0	0.6610228296370414	0.0
7	0.0	0.0	0.0	0.7614313607211489	0.0
8	0.0	0.0	0.0	0.9596576867316848	0.0
9	0.0	0.0	0.0	1.0246361503621253	0.0
10	0.0	0.0	0.0	1.1573588635210694	0.0
11	0.0	0.0	0.0	1.3879693915025955	0.0
12	0.0	0.0	0.0	1.545584177744106	0.0
13	0.0	0.0	0.0	1.6059063182803393	0.0
14	0.0	0.0	0.0	1.6343343961186572	0.0
15	0.0	0.0	0.0	1.6820879652953387	0.0
16	0.0	0.0	0.0	1.7965354855582214	0.0
17	0.0	0.0	0.0	1.923551577180656	0.0
18	0.0	0.0	0.0	2.07090844863568	0.0
19	0.0	0.0	0.0	2.168726243562108	0.0
20	0.0	0.0	0.0	2.273511018155809	0.0
21	0.0	0.0	0.0	2.409979795758965	3.5009948076746006E-5
22	0.0	0.0	0.0	2.5585270054485982	3.5009948076746006E-5
23	3.5009948076746006E-5	0.0	0.0	2.7108202795824434	3.5009948076746006E-5
24	3.5009948076746006E-5	0.0	0.0	2.821556745349191	3.5009948076746006E-5
25	3.5009948076746006E-5	0.0	0.0	2.932958400129397	3.5009948076746006E-5
26	3.5009948076746006E-5	0.0	0.0	3.033576990901965	3.5009948076746006E-5
27	3.5009948076746006E-5	0.0	0.0	3.1429130587456426	3.5009948076746006E-5
28	3.5009948076746006E-5	0.0	0.0	3.258901016723902	3.5009948076746006E-5
29	3.5009948076746006E-5	0.0	0.0	3.4040172515020144	3.5009948076746006E-5
30	7.001989615349201E-5	0.0	0.0	3.5522493716589567	3.5009948076746006E-5
31	1.0502984423023802E-4	0.0	0.0	3.6976806959697597	3.5009948076746006E-5
32	1.0502984423023802E-4	0.0	0.0	3.8227712404479735	3.5009948076746006E-5
33	1.0502984423023802E-4	0.0	0.0	3.9469515262761914	3.5009948076746006E-5
34	1.0502984423023802E-4	0.0	0.0	4.086221099725487	3.5009948076746006E-5
35	1.0502984423023802E-4	0.0	0.0	4.243450776538153	3.5009948076746006E-5
36	1.0502984423023802E-4	0.0	0.0	4.386816513912428	3.5009948076746006E-5
37	1.0502984423023802E-4	0.0	0.0	4.5347685544847565	3.5009948076746006E-5
38	1.0502984423023802E-4	0.0	0.0	4.701520937174298	3.5009948076746006E-5
39	1.0502984423023802E-4	0.0	0.0	4.956288329328779	3.5009948076746006E-5
40	1.0502984423023802E-4	0.0	0.0	5.126261627241381	3.5009948076746006E-5
41	1.0502984423023802E-4	0.0	0.0	5.2984755618308945	3.5009948076746006E-5
42	1.0502984423023802E-4	0.0	0.0	5.475135759826155	3.5009948076746006E-5
43	1.0502984423023802E-4	0.0	0.0	5.637616928850333	3.5009948076746006E-5
44	1.0502984423023802E-4	0.0	0.0	5.809130664478311	3.5009948076746006E-5
45	1.0502984423023802E-4	0.0	0.0	5.977003365506309	3.5009948076746006E-5
46	1.0502984423023802E-4	0.0	0.0	6.165426906055355	3.5009948076746006E-5
47	1.0502984423023802E-4	0.0	0.0	6.364983610092808	3.5009948076746006E-5
48	1.0502984423023802E-4	0.0	0.0	6.548750827547647	3.5009948076746006E-5
49	1.0502984423023802E-4	0.0	0.0	6.744631487037042	3.5009948076746006E-5
50	1.4003979230698402E-4	0.0	0.0	6.948739484324471	3.5009948076746006E-5
51	1.4003979230698402E-4	0.0	0.0	7.161880048215701	3.5009948076746006E-5
52	1.4003979230698402E-4	0.0	0.0	7.406984694700999	3.5009948076746006E-5
53	1.4003979230698402E-4	0.0	0.0	7.6190399502018495	3.5009948076746006E-5
54	1.7504974038373003E-4	0.0	0.0	7.832180514093079	3.5009948076746006E-5
55	1.7504974038373003E-4	0.0	0.0	8.049452251857366	3.5009948076746006E-5
56	2.1005968846047604E-4	0.0	0.0	8.275161387108147	3.5009948076746006E-5
57	2.1005968846047604E-4	0.0	0.0	8.502060860593538	3.5009948076746006E-5
58	2.1005968846047604E-4	0.0	0.0	8.701092415409839	3.5009948076746006E-5
59	2.4506963653722207E-4	0.0	0.0	8.920639799799114	3.5009948076746006E-5
60	2.4506963653722207E-4	0.0	0.0	9.161298182878665	3.5009948076746006E-5
61	2.4506963653722207E-4	0.0	0.0	9.398525591046695	3.5009948076746006E-5
62	2.4506963653722207E-4	0.0	0.0	9.680985852129883	3.5009948076746006E-5
63	2.4506963653722207E-4	0.0	0.0	9.960190188041931	3.5009948076746006E-5
64	2.4506963653722207E-4	0.0	0.0	10.227316091867504	3.5009948076746006E-5
65	3.150895326907141E-4	0.0	0.0	10.474556345185485	3.5009948076746006E-5
66	3.150895326907141E-4	0.0	0.0	10.715879917278494	3.5009948076746006E-5
67	3.5009948076746006E-4	0.0	0.0	10.968511702600294	3.5009948076746006E-5
68	3.5009948076746006E-4	0.0	0.0	11.26077474914497	3.5009948076746006E-5
69	3.851094288442061E-4	0.0	0.0	11.6819794344563	3.5009948076746006E-5
70	3.851094288442061E-4	0.0	0.0	11.956947566651063	3.5009948076746006E-5
71	3.851094288442061E-4	0.0	0.0	12.215811122730523	3.5009948076746006E-5
72	3.851094288442061E-4	0.0	0.0	12.479190962111884	3.5009948076746006E-5
73	3.851094288442061E-4	0.0	0.0	12.751148238772046	3.5009948076746006E-5
74	3.851094288442061E-4	0.0	0.0	13.052758941453215	3.5009948076746006E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	20	4.0919418E-5	80.0	3
GCGCGAC	140	0.0	74.28571	9
CGTTAGG	470	0.0	72.34042	2
GTTAGCG	135	0.0	71.11111	1
TATAGCG	165	0.0	70.30303	1
CGTTTTT	7780	0.0	68.79177	1
TAGCGCG	140	0.0	68.57143	1
TAGGGTA	2825	0.0	67.539825	5
TAACGCG	90	0.0	66.66667	1
TTCGTAG	270	0.0	66.666664	1
TACGGGT	745	0.0	66.04027	4
CGGGATC	620	0.0	65.80645	6
TACGCGG	110	0.0	65.454544	2
GTACGGG	865	0.0	64.27746	3
CGAGGGT	910	0.0	64.17582	4
ACGGGTA	800	0.0	63.0	5
AGGGTAC	2115	0.0	62.978725	6
CGAGGGA	1620	0.0	62.962967	4
GCGAGGG	2230	0.0	62.600895	3
CGGGTGC	730	0.0	61.917805	6
>>END_MODULE