FastQCFastQC Report
Thu 26 May 2016
SRR1547793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547793_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4534282
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257100.5670136969866453No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC94530.20847843164584823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127950.069.9960861
CGAGGGA31900.067.4608154
TAACGGG11650.067.296143
CGTTAGG7750.067.096772
TAATGCG3650.065.7534261
CGTCGGG2650.064.905663
TTAGGGA81450.064.874154
TACGGGA22000.064.727274
CGAACGG2250.064.02
TAGGGAC60200.063.720935
TAGGGAG65650.063.549125
ACGGGAT27050.062.9944575
CGGGATC15500.062.9677436
TAAGGGA60700.062.800664
AGGGATT114200.062.7670756
AGGGACC54350.062.55756
GTAGGGA86800.062.304154
TATAGCG2450.062.0408131
GGGCGAT26450.062.0037847
TCGTAGG5300.061.8867952
Produced by FastQC (version 0.11.5)