FastQCFastQC Report
Thu 26 May 2016
SRR1547792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547792_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2927647
Sequences flagged as poor quality0
Sequence length86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT594012.0289672901138696No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGC32170.10988346614192217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG150.001309994480.000016
CGTTTTT242400.075.181521
TATAGCG1900.071.578951
CGGGATC11800.069.152546
CGAAACG2450.068.5714380
CTCGCGG950.067.368422
CCGATCC17600.067.2727380
CGAGGGA24400.065.737714
GGGCGAT21350.065.386417
TACGGGA15850.065.362784
TAGTGCG3250.065.230771
TAGCGGG21800.064.587163
TATTGCG3350.064.4776151
CGCGGGA9350.064.171124
TAGGGAC37600.064.148945
AGGGCGA11200.063.571436
TGGGCGA5350.063.5514036
ATAGCGG5500.063.2727282
CGCGAGG3250.062.769232
GCGGGAT26150.062.7151075
Produced by FastQC (version 0.11.5)