##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547792_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2927647
Sequences flagged as poor quality	0
Sequence length	86
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.844962182940773	31.0	31.0	33.0	30.0	34.0
2	32.29604149680614	33.0	31.0	34.0	31.0	34.0
3	32.36060529155325	34.0	31.0	34.0	30.0	34.0
4	35.987487562537424	37.0	35.0	37.0	35.0	37.0
5	35.96328997314225	37.0	35.0	37.0	35.0	37.0
6	36.02345603824505	37.0	35.0	37.0	35.0	37.0
7	36.29817939116294	37.0	36.0	37.0	35.0	37.0
8	36.27321770691617	37.0	37.0	37.0	35.0	37.0
9	38.14796285207882	39.0	39.0	39.0	37.0	39.0
10-11	37.6058419952952	39.0	37.5	39.0	35.0	39.0
12-13	37.128342146440474	39.0	37.0	39.0	33.5	39.0
14-15	38.334607280180975	40.0	37.0	41.0	33.5	41.0
16-17	38.421156478222954	40.0	37.0	41.0	34.0	41.0
18-19	38.17387427514315	40.0	37.0	41.0	34.0	41.0
20-21	37.88593638509015	40.0	35.0	41.0	34.0	41.0
22-23	37.70634284119636	39.5	35.0	41.0	33.0	41.0
24-25	37.600700494287736	39.0	35.0	41.0	33.0	41.0
26-27	37.53418786486212	39.0	35.0	41.0	33.0	41.0
28-29	37.47180893051655	40.0	35.0	41.0	33.0	41.0
30-31	37.20174614630794	39.0	35.0	41.0	33.0	41.0
32-33	36.66471060206371	39.0	35.0	41.0	31.5	41.0
34-35	36.17110105829016	39.0	35.0	41.0	29.5	41.0
36-37	35.91036965863712	39.0	35.0	41.0	28.5	41.0
38-39	35.78208148045171	39.0	35.0	41.0	27.0	41.0
40-41	35.63103253226909	39.0	35.0	41.0	26.5	41.0
42-43	35.55587097761445	39.0	35.0	41.0	26.0	41.0
44-45	35.44309184133196	39.0	35.0	41.0	25.0	41.0
46-47	35.350195737395936	38.0	35.0	41.0	25.0	41.0
48-49	35.223933930559255	38.0	35.0	40.5	24.0	41.0
50-51	35.055254612321775	38.0	35.0	40.0	24.0	41.0
52-53	34.86258742942711	38.0	34.5	40.0	24.0	41.0
54-55	34.6815931019006	37.0	34.0	40.0	23.5	41.0
56-57	34.421101997611046	37.0	34.0	40.0	23.0	41.0
58-59	34.1405888414826	36.5	34.0	40.0	22.0	41.0
60-61	33.850522279496126	36.0	34.0	39.5	22.0	41.0
62-63	33.51819584123359	35.5	33.0	39.0	20.5	41.0
64-65	33.20260775974699	35.0	33.0	39.0	20.0	40.5
66-67	32.89990801486655	35.0	33.0	38.5	19.5	40.0
68-69	32.44321600247571	35.0	32.5	37.5	17.5	40.0
70-71	32.12915030398132	35.0	32.0	37.0	16.0	39.5
72-73	31.817328557712045	35.0	32.0	36.5	15.0	39.0
74-75	31.498001808278115	35.0	32.0	36.0	13.0	39.0
76-77	29.467211723271284	33.0	28.0	34.5	10.0	36.0
78-79	30.58567802060836	34.0	30.5	35.0	10.0	37.0
80-81	30.7001195840892	35.0	31.0	35.0	10.0	37.0
82-83	30.500462658237144	35.0	31.0	35.0	10.0	36.0
84-85	30.26808696540259	34.0	31.0	35.0	10.0	36.0
86	29.85281866290574	34.0	30.0	35.0	10.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	7.0
10	10.0
11	18.0
12	46.0
13	159.0
14	554.0
15	1778.0
16	4956.0
17	12049.0
18	27807.0
19	42286.0
20	44486.0
21	38981.0
22	31520.0
23	25355.0
24	23145.0
25	24508.0
26	27699.0
27	32895.0
28	39438.0
29	48677.0
30	60232.0
31	75064.0
32	93802.0
33	121234.0
34	252690.0
35	269466.0
36	255360.0
37	413083.0
38	618807.0
39	340173.0
40	1354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.27239759438211	23.425160205448268	30.579233083770003	17.723209116399623
2	33.48313509108168	24.590362157732816	29.84454751546208	12.081955235723433
3	26.881109641975282	23.420378208165125	37.2516222071855	12.4468899426741
4	23.81007682961778	26.134093352101534	36.68020768897343	13.375622129307255
5	22.94839507631897	29.941178017705006	33.96444311762996	13.145983788346069
6	21.566397861490817	38.088164317624354	30.083579065372295	10.261858755512533
7	84.79458076742176	3.1139683165354293	10.841880868834256	1.2495700472085602
8	85.98300956365298	2.4726683237425826	10.48777397001756	1.0565481425868626
9	81.40438379353795	5.464866495175135	11.396285139567714	1.7344645717192
10-11	32.344592773650646	30.46292807841929	25.549818676910157	11.642660471019902
12-13	24.815782093947803	25.298388091187224	33.8734826978799	16.012347116985072
14-15	15.869706969453626	30.504172804986396	37.741401200349635	15.884719025210348
16-17	20.125667472888637	27.814265176095343	35.32983997046092	16.730227380555103
18-19	20.966410909511975	29.57759251713065	33.846344863298064	15.609651710059309
20-21	22.742017736427922	29.01608356471938	33.78520361232075	14.456695086531948
22-23	19.659251952164997	26.01174253589999	35.64734751149985	18.68165800043516
24-25	17.883918382236658	29.017586478151223	36.409495407062394	16.68899973254972
26-27	17.01028163572999	33.6309158856925	33.12352889538937	16.235273583188135
28-29	15.661024023729636	30.048209364038765	37.55546689884402	16.735299713387576
30-31	19.376243105811593	29.70933654228122	34.35060647680543	16.563813875101747
32-33	21.19171129579488	29.651132803920692	32.480196553751185	16.67695934653324
34-35	18.11897062726483	30.48072393973727	32.99532013251598	18.404985300481925
36-37	20.04503958298251	30.83466005293671	32.36308885599937	16.757211508081404
38-39	18.546839834173998	31.96560241039989	30.978444464103767	18.50911329132235
40-41	18.91312374750098	30.131057466969207	31.22285576095752	19.732963024572292
42-43	19.23447396492815	30.133465544172505	30.805148298275032	19.826912192624317
44-45	18.371169748265416	32.23546076422465	30.955166384471898	18.438203103038038
46-47	19.578811926437854	31.725870639458925	30.536485443771056	18.158831990332168
48-49	19.2672477248794	30.486411100791866	31.69584652794548	18.550494646383257
50-51	18.109440789822	31.719073371892172	31.212215817002527	18.959270021283302
52-53	19.649295150679027	29.731743615265096	31.466532679657078	19.152428554398806
54-55	19.046524393138927	30.33615391473084	31.879492302179873	18.73782938995036
56-57	19.876354628819662	28.839252136613464	31.748158162510716	19.53623507205616
58-59	20.223869202810313	30.28037533213533	30.73724393685441	18.75851152819995
60-61	20.231725341204047	31.64324455783091	29.59776229852848	18.527267802436565
62-63	20.22830962885894	30.08480872181653	31.16567673630052	18.521204913024008
64-65	18.8886330899866	32.53042460378591	30.87136188208483	17.709580424142665
66-67	17.873739559448254	32.304372760787075	28.72021456138667	21.101673118378
68-69	17.32642289183088	33.238467615802044	28.9522268224277	20.482882669939375
70-71	18.286716260532778	32.354105532531754	29.703751852596984	19.655426354338484
72-73	19.627622455849355	32.05514530952673	29.577131395964063	18.740100838659853
74-75	18.804452859241568	32.915016735282634	28.091347761529995	20.189182643945802
76-77	21.036364698339657	31.22357306054999	29.01319728778777	18.726864953322583
78-79	17.848651152273483	32.08231730123201	30.376630106020297	19.69240144047421
80-81	16.83631940599396	31.113382180297013	30.88160901912013	21.168689394588895
82-83	17.80282937116394	30.597472987692846	31.447780418882466	20.151917222260742
84-85	16.947005564536983	30.48150955357664	30.54270204023914	22.028782841647235
86	17.939970221819777	29.846357843004977	31.159460139832433	21.054211795342813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2218.0
1	2913.5
2	19817.0
3	21725.0
4	7934.5
5	10592.5
6	14929.0
7	17117.0
8	18372.5
9	19477.0
10	19013.5
11	17909.5
12	16477.0
13	15739.5
14	15643.0
15	15669.5
16	15775.0
17	15837.0
18	15894.5
19	16246.0
20	16869.0
21	17215.0
22	18775.5
23	20343.5
24	22284.0
25	25191.0
26	29758.0
27	39234.0
28	49548.5
29	53980.0
30	56659.5
31	63844.0
32	70012.5
33	75411.0
34	83565.0
35	89298.5
36	90613.0
37	93640.0
38	98695.5
39	104039.0
40	113566.0
41	127658.0
42	136412.0
43	137286.0
44	140912.0
45	142359.5
46	139145.5
47	136583.0
48	131936.5
49	118212.5
50	107608.0
51	98930.0
52	80717.5
53	66470.0
54	57938.0
55	49971.0
56	41924.5
57	38026.0
58	32761.5
59	24292.0
60	18823.0
61	15013.0
62	11699.5
63	7984.5
64	6037.0
65	4893.0
66	2735.5
67	1407.0
68	926.5
69	593.5
70	377.5
71	329.0
72	260.0
73	142.5
74	105.0
75	97.5
76	66.0
77	44.0
78	26.5
79	18.0
80	16.5
81	15.0
82	10.5
83	4.5
84	5.0
85	5.0
86	3.0
87	2.5
88	2.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2927647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.478543284912853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65602298647951	15.321047483612416
2	7.55348002307977	2.942615751625677
3	2.7927371018758094	1.6319535156681004
4	1.6175924668131942	1.2603337952867903
5	1.1151226062689166	1.0860481977096963
6	0.8258055129975295	0.9651293057905223
7	0.6691769112802398	0.9124214002145508
8	0.534747660530374	0.8332884361717421
9	0.4030747164841697	0.7066177480881788
>10	3.5195339420270004	15.362407409168366
>50	1.0801439211947292	15.286028178429564
>100	1.2252982544175939	40.2630783829502
>500	0.004960709839905091	0.6632780906300394
>1k	0.002126018502816467	0.7158364950020244
>5k	0.0	0.0
>10k+	1.7716820856803893E-4	2.0499158096521835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59401	2.0289672901138696	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGC	3217	0.10988346614192217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.83142469020343E-5	0.0	0.0	0.02633514218073422	0.0
2	6.83142469020343E-5	0.0	0.0	0.07968856901122301	0.0
3	6.83142469020343E-5	0.0	0.0	0.1205063315351885	0.0
4	6.83142469020343E-5	0.0	0.0	0.17833434153776054	0.0
5	6.83142469020343E-5	0.0	0.0	0.320667074958149	0.0
6	6.83142469020343E-5	0.0	0.0	0.46566406400771676	0.0
7	6.83142469020343E-5	0.0	0.0	0.585555567320787	0.0
8	6.83142469020343E-5	0.0	0.0	0.81669682171382	0.0
9	6.83142469020343E-5	0.0	0.0	0.9343339548791231	0.0
10	6.83142469020343E-5	0.0	0.0	1.1297468581423922	0.0
11	6.83142469020343E-5	0.0	0.0	1.5064657726836603	0.0
12	6.83142469020343E-5	0.0	0.0	1.7653084541954682	0.0
13	6.83142469020343E-5	0.0	0.0	1.8549709032543882	0.0
14	6.83142469020343E-5	0.0	0.0	1.8939100239885478	0.0
15	6.83142469020343E-5	0.0	0.0	1.9445991951898574	0.0
16	6.83142469020343E-5	0.0	0.0	2.0519550341964043	0.0
17	6.83142469020343E-5	0.0	0.0	2.1919992403455746	0.0
18	6.83142469020343E-5	0.0	0.0	2.342119797912795	0.0
19	6.83142469020343E-5	0.0	0.0	2.454189319955582	0.0
20	6.83142469020343E-5	0.0	0.0	2.5605204452585983	0.0
21	6.83142469020343E-5	0.0	0.0	2.689156172175129	0.0
22	6.83142469020343E-5	0.0	0.0	2.84231671372949	0.0
23	6.83142469020343E-5	0.0	0.0	3.0043922645045664	0.0
24	6.83142469020343E-5	0.0	0.0	3.1220977119167714	0.0
25	6.83142469020343E-5	0.0	0.0	3.2254571674795494	0.0
26	6.83142469020343E-5	0.0	0.0	3.326459781524207	0.0
27	6.83142469020343E-5	0.0	0.0	3.4356259480736577	0.0
28	6.83142469020343E-5	0.0	0.0	3.5463633423018552	0.0
29	6.83142469020343E-5	0.0	0.0	3.672471442083011	0.0
30	6.83142469020343E-5	0.0	0.0	3.814189347281281	0.0
31	1.0247137035305144E-4	0.0	0.0	3.9487000994313863	0.0
32	1.0247137035305144E-4	0.0	0.0	4.074500785101483	0.0
33	1.0247137035305144E-4	0.0	0.0	4.1996183283025585	0.0
34	1.0247137035305144E-4	0.0	0.0	4.331259882082779	0.0
35	1.0247137035305144E-4	0.0	0.0	4.4791260695022315	0.0
36	1.0247137035305144E-4	0.0	0.0	4.618726233046539	0.0
37	1.0247137035305144E-4	0.0	0.0	4.7709645322677225	0.0
38	1.0247137035305144E-4	0.0	0.0	4.932322783450327	0.0
39	1.0247137035305144E-4	0.0	0.0	5.128999500281284	0.0
40	1.0247137035305144E-4	0.0	0.0	5.306001714004455	0.0
41	1.0247137035305144E-4	0.0	0.0	5.485292454998844	0.0
42	1.0247137035305144E-4	0.0	0.0	5.6665301520299405	0.0
43	1.0247137035305144E-4	0.0	0.0	5.837657340519537	0.0
44	1.0247137035305144E-4	0.0	0.0	6.0091602573670935	0.0
45	1.0247137035305144E-4	0.0	0.0	6.1761202767956656	0.0
46	1.0247137035305144E-4	0.0	0.0	6.360534586307707	0.0
47	1.0247137035305144E-4	0.0	0.0	6.55574254683027	0.0
48	1.0247137035305144E-4	0.0	0.0	6.744255711156434	0.0
49	1.0247137035305144E-4	0.0	0.0	6.94223039867853	0.0
50	1.0247137035305144E-4	0.0	0.0	7.142220356484235	0.0
51	1.0247137035305144E-4	0.0	0.0	7.347675454042102	0.0
52	1.0247137035305144E-4	0.0	0.0	7.572053597991834	0.0
53	1.0247137035305144E-4	0.0	0.0	7.780890250771353	0.0
54	1.0247137035305144E-4	0.0	0.0	7.994850472068524	0.0
55	1.0247137035305144E-4	0.0	0.0	8.201330283329924	0.0
56	1.0247137035305144E-4	0.0	0.0	8.41713498929345	0.0
57	1.0247137035305144E-4	0.0	0.0	8.646705016007736	0.0
58	1.0247137035305144E-4	0.0	0.0	8.86175826525534	0.0
59	1.0247137035305144E-4	0.0	0.0	9.093377719376686	0.0
60	1.0247137035305144E-4	0.0	0.0	9.336747223965185	0.0
61	1.0247137035305144E-4	0.0	0.0	9.577213373060346	0.0
62	1.0247137035305144E-4	0.0	0.0	9.846166563113654	0.0
63	1.0247137035305144E-4	0.0	0.0	10.10763934313119	0.0
64	1.0247137035305144E-4	0.0	0.0	10.363612826273112	0.0
65	1.0247137035305144E-4	0.0	0.0	10.61470184076154	0.0
66	1.0247137035305144E-4	0.0	0.0	10.861657843312393	0.0
67	1.0247137035305144E-4	0.0	0.0	11.115308642059647	0.0
68	1.0247137035305144E-4	0.0	0.0	11.382349033199699	0.0
69	1.0247137035305144E-4	0.0	0.0	11.693793684826074	0.0
70	1.0247137035305144E-4	0.0	0.0	11.968416957372252	0.0
71	1.0247137035305144E-4	0.0	0.0	12.24577279979451	0.0
72	1.366284938040686E-4	0.0	0.0	12.532419379795448	0.0
73	1.366284938040686E-4	0.0	0.0	12.8112439785261	0.0
74	1.366284938040686E-4	0.0	0.0	13.110187122969402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	15	0.0013099944	80.00001	6
CGTTTTT	24240	0.0	75.18152	1
TATAGCG	190	0.0	71.57895	1
CGGGATC	1180	0.0	69.15254	6
CGAAACG	245	0.0	68.57143	80
CTCGCGG	95	0.0	67.36842	2
CCGATCC	1760	0.0	67.27273	80
CGAGGGA	2440	0.0	65.73771	4
GGGCGAT	2135	0.0	65.38641	7
TACGGGA	1585	0.0	65.36278	4
TAGTGCG	325	0.0	65.23077	1
TAGCGGG	2180	0.0	64.58716	3
TATTGCG	335	0.0	64.477615	1
CGCGGGA	935	0.0	64.17112	4
TAGGGAC	3760	0.0	64.14894	5
AGGGCGA	1120	0.0	63.57143	6
TGGGCGA	535	0.0	63.551403	6
ATAGCGG	550	0.0	63.272728	2
CGCGAGG	325	0.0	62.76923	2
GCGGGAT	2615	0.0	62.715107	5
>>END_MODULE