FastQCFastQC Report
Thu 26 May 2016
SRR1547790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547790_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2811875
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166840.5933407423871971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79000.069.063291
TAGGGAC35450.066.008465
GTAAGCG3100.065.806451
TAAGGGA46850.065.144074
CGAGGGA19100.065.130894
TGCGGGC3750.065.0666664
GACCGAT5350.065.046739
TCGCGCG503.0013325E-1064.01
TAGGGAG38300.063.916455
CGGGACT13150.063.878336
TACGGGA14950.063.6789324
ACGGGAC10750.063.6279075
CGAACGG1450.063.448282
AGGGATT71150.063.3028876
CTAGCGG3300.063.0303042
ATTAGCG3150.062.2222251
CCGAGGG6200.061.9354823
AGCGGGA26650.061.8386464
TAGCGGG20350.061.326783
CGACCGG850.061.176472
Produced by FastQC (version 0.11.5)