##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547790_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2811875
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71584440986886	31.0	31.0	33.0	30.0	34.0
2	32.14720569015337	33.0	31.0	34.0	30.0	34.0
3	32.29090268948655	34.0	31.0	34.0	30.0	34.0
4	35.88574207601689	37.0	35.0	37.0	35.0	37.0
5	35.81990415647922	37.0	35.0	37.0	35.0	37.0
6	35.88373629695488	37.0	35.0	37.0	35.0	37.0
7	36.21988530784619	37.0	35.0	37.0	35.0	37.0
8	36.19643085130029	37.0	35.0	37.0	35.0	37.0
9	38.07198150700155	39.0	39.0	39.0	37.0	39.0
10-11	37.436868281840404	39.0	37.0	39.0	34.5	39.0
12-13	36.80137274949989	39.0	35.0	39.0	33.0	39.0
14-15	37.89066032451656	40.0	36.0	41.0	33.0	41.0
16-17	38.03881378084019	40.0	36.0	41.0	33.0	41.0
18-19	37.94235216714826	40.0	36.0	41.0	33.5	41.0
20-21	37.770585107801736	40.0	35.0	41.0	33.0	41.0
22-23	37.58378181818182	39.5	35.0	41.0	33.0	41.0
24-25	37.508275083351855	39.0	35.0	41.0	33.0	41.0
26-27	37.486686641475885	39.0	35.0	41.0	33.0	41.0
28-29	37.46669002000445	39.5	35.0	41.0	33.0	41.0
30-31	37.306080640142255	39.0	35.0	41.0	32.5	41.0
32-33	37.02907579462102	39.0	35.0	41.0	32.0	41.0
34-35	36.78044916648144	39.0	35.0	41.0	31.0	41.0
36-37	36.594117803956436	39.0	35.0	41.0	31.0	41.0
38-39	36.50014616581463	39.0	35.0	41.0	31.0	41.0
40-41	36.36102902867304	39.0	35.0	41.0	30.0	41.0
42-43	36.325061569237604	39.0	35.0	41.0	30.0	41.0
44-45	36.21702476105801	38.5	35.0	41.0	30.0	41.0
46-47	36.14076390308958	38.0	35.0	41.0	30.0	41.0
48-49	36.041685707935095	38.0	35.0	40.5	30.0	41.0
50-51	35.872903667481665	38.0	35.0	40.0	29.0	41.0
52-53	35.703303845298954	38.0	35.0	40.0	29.0	41.0
54-55	35.521875794621025	37.5	35.0	40.0	28.5	41.0
56-57	35.224790753500784	37.0	34.0	40.0	28.0	41.0
58-59	34.92801369193154	36.5	34.0	40.0	27.0	41.0
60-61	34.61059986663703	36.0	34.0	39.5	26.5	41.0
62-63	34.213778706379195	35.5	33.5	39.0	26.0	41.0
64-65	33.86954469882196	35.0	33.0	39.0	25.5	40.0
66-67	33.53919519893309	35.0	33.0	38.0	25.0	40.0
68-69	33.07615701266948	35.0	33.0	37.5	23.0	40.0
70-71	32.76568410757946	35.0	33.0	37.0	22.5	39.5
72-73	32.44775443431874	35.0	32.5	36.5	21.5	39.0
74-75	32.12654065347856	35.0	32.0	36.0	20.5	38.5
76-77	30.020856901533676	33.0	29.0	34.5	18.5	36.0
78-79	31.125691575905755	34.0	31.0	35.0	19.0	37.0
80-81	31.30397332740609	35.0	31.5	35.0	19.0	37.0
82-83	31.100720871304734	34.5	31.0	35.0	18.0	36.0
84-85	30.872135852411645	34.0	31.0	35.0	17.5	36.0
86	30.456554789953323	34.0	31.0	35.0	15.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	9.0
10	10.0
11	19.0
12	39.0
13	88.0
14	326.0
15	1110.0
16	2630.0
17	5776.0
18	10984.0
19	17353.0
20	20335.0
21	20064.0
22	19228.0
23	18875.0
24	20890.0
25	24856.0
26	30250.0
27	37059.0
28	45541.0
29	56419.0
30	69492.0
31	84110.0
32	102847.0
33	130746.0
34	279932.0
35	268765.0
36	249395.0
37	409916.0
38	586411.0
39	297367.0
40	1025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.135238941987108	23.926703711935986	32.14143587463881	12.796621471438097
2	29.24312069348744	25.507712825072236	32.43785730162258	12.811309179817737
3	28.632460546788174	25.076185819070908	33.648117359413206	12.643236274727718
4	26.394629917759506	27.691131362525006	31.92101355856857	13.993225161146922
5	23.740493442987333	31.594825516781505	30.977017114914425	13.687663925316738
6	22.34871749277617	40.780689042009335	26.70669926650367	10.163894198710825
7	89.74282284952211	3.1689886641475886	5.745667926205823	1.342520560124472
8	90.97374527672815	2.3693798621915985	5.186610357857302	1.4702645032229384
9	86.5299133140698	5.444232051567015	6.310949099799956	1.7149055345632362
10-11	35.8414581018004	32.8454678817515	20.272202711713714	11.040871304734386
12-13	27.167334963325185	26.74012891753723	29.842507223827518	16.25002889531007
14-15	15.916159146476993	32.894349855523444	34.65033118470771	16.539159813291842
16-17	20.653051789286508	28.0097443876417	32.76121804845521	18.575985774616584
18-19	22.445467881751497	30.040062236052457	30.52200044454323	16.99246943765281
20-21	24.83206045787953	29.599413202933984	29.783187374972215	15.78533896421427
22-23	20.187828406312512	26.602356079128697	32.65216270282285	20.55765281173594
24-25	18.446285841298067	29.769655478995332	33.99381195821294	17.790246721493666
26-27	17.160293398533007	34.926499222049344	29.706885974661034	18.20632140475661
28-29	16.017817292731717	30.50599688819738	34.83170482329406	18.64448099577684
30-31	21.027197154923314	30.124543231829293	30.658421871527008	18.189837741720382
32-33	23.388077350522337	30.144174260946876	28.85770171149144	17.610046677039342
34-35	19.088010669037565	30.323129584352078	30.390468993109582	20.198390753500778
36-37	21.33231384752167	30.13611913758613	30.75421204712158	17.777354967770613
38-39	18.91783951989331	32.32280951322516	29.084863302956208	19.674487663925316
40-41	20.26124916648144	28.440097799511005	30.21362969548789	21.08502333851967
42-43	19.997172705045564	28.346103578573018	29.58648588575239	22.07023783062903
44-45	19.147970660146697	31.7940697932874	29.050562347188265	20.00739719937764
46-47	20.448721938208493	30.72442765058902	28.89708824183152	19.92976216937097
48-49	20.120346743720827	28.874363191820407	30.99166925983552	20.01362080462325
50-51	18.531780395643477	31.60478328517448	30.01646588130696	19.846970437875083
52-53	20.687797288286287	28.576430317848413	30.452278284063127	20.283494109802177
54-55	20.954309846632587	28.84200044454323	31.001182484996665	19.202507223827517
56-57	21.0723449655479	27.497683929762168	30.35033563014003	21.0796354745499
58-59	21.7478728606357	28.833198488553013	29.70473438541898	19.714194265392308
60-61	22.421871527006	30.431687041564793	27.81805734607691	19.3283840853523
62-63	21.664512113803067	29.02815736830407	29.98773060680151	19.319599911091352
64-65	19.383916425872417	31.33919537675039	30.301649255390085	18.975238941987108
66-67	18.205058901978216	31.17651033563014	27.729824405423425	22.888606356968218
68-69	17.603396310291174	32.50709935541231	27.356496999333185	22.533007334963326
70-71	19.235954656590355	31.592727272727274	28.89372749499889	20.277590575683487
72-73	21.50460102244943	30.82007557234941	27.90136474772172	19.77395865747944
74-75	20.146610357857302	32.55311847077128	26.48929984440987	20.810971326961546
76-77	23.116176928206265	30.468086241386978	26.98829073127362	19.427446099133142
78-79	18.669482107134918	30.79978661924872	29.90017337186041	20.630557901755946
80-81	16.812802845076682	29.959564347632806	30.90891309179818	22.318719715492332
82-83	18.777701711491442	28.53380751278062	31.409931095799067	21.278559679928875
84-85	17.02749944432096	28.69542565014448	30.321475883529676	23.95559902200489
86	17.857977328295178	28.662867303845296	31.123894198710822	22.355261169148697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2119.0
1	2520.5
2	7015.5
3	7128.0
4	3527.5
5	5082.0
6	7673.5
7	9091.0
8	10068.0
9	11283.0
10	11053.5
11	9953.0
12	8835.0
13	8140.5
14	7931.0
15	7808.5
16	7386.5
17	7264.5
18	7696.0
19	9145.5
20	9392.5
21	8444.0
22	10430.5
23	12706.5
24	15683.0
25	19770.5
26	22736.5
27	28352.0
28	36186.0
29	39970.0
30	45086.0
31	53098.5
32	59945.0
33	65988.5
34	74552.0
35	86776.5
36	92531.0
37	91681.0
38	97153.5
39	103597.5
40	115181.0
41	135491.5
42	146187.0
43	148034.0
44	152242.5
45	156526.0
46	156514.0
47	157537.0
48	153439.0
49	133291.0
50	118351.0
51	104538.5
52	82047.0
53	68879.0
54	60391.0
55	52827.0
56	45319.0
57	41376.0
58	37391.0
59	27488.5
60	19171.0
61	14266.0
62	9968.5
63	6485.5
64	4795.0
65	4090.0
66	2669.5
67	1509.5
68	1038.0
69	731.0
70	321.0
71	191.0
72	147.5
73	89.5
74	77.5
75	52.0
76	18.5
77	19.0
78	57.0
79	89.0
80	61.0
81	18.5
82	5.0
83	4.5
84	2.5
85	1.5
86	1.0
87	1.5
88	1.0
89	0.5
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2811875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.0969847947312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.40059482281866	13.233167927888811
2	9.542853755849357	3.2630805112420003
3	3.157700055065138	1.619614494833116
4	1.5775576799740054	1.07885918669308
5	0.9642293449943714	0.8242707225001195
6	0.7197198785205159	0.7383023891718626
7	0.5328377419100794	0.6376943140046873
8	0.40602570429551066	0.5553452234088299
9	0.3527674498941529	0.542813375422479
>10	2.7720723417389417	10.289470020348935
>50	0.9267351574885878	11.558346593479062
>100	1.6330700446359954	53.01489610691485
>500	0.010272198756472313	1.1561410820816826
>1k	0.003354187349052184	0.890017526436195
>5k	0.0	0.0
>10k+	2.096367093157615E-4	0.5979805255743186
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16684	0.5933407423871971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.112691709268727E-5	0.0	0.0	0.08730829073127362	0.0
2	7.112691709268727E-5	0.0	0.0	0.25303400755723493	0.0
3	7.112691709268727E-5	0.0	0.0	0.3548166259168704	0.0
4	7.112691709268727E-5	0.0	0.0	0.5084507668370749	0.0
5	7.112691709268727E-5	0.0	0.0	0.8518515225605691	0.0
6	7.112691709268727E-5	0.0	0.0	1.1011513669704378	0.0
7	7.112691709268727E-5	0.0	0.0	1.2914158701933762	0.0
8	1.0669037563903089E-4	0.0	0.0	1.701426983774172	0.0
9	1.0669037563903089E-4	0.0	0.0	1.8228406312513892	0.0
10	1.0669037563903089E-4	0.0	0.0	2.064814403200711	0.0
11	1.0669037563903089E-4	0.0	0.0	2.50348966436986	0.0
12	1.0669037563903089E-4	0.0	0.0	2.830460102244943	0.0
13	1.0669037563903089E-4	0.0	0.0	2.939746610357857	0.0
14	1.0669037563903089E-4	0.0	0.0	2.982067126028006	0.0
15	1.0669037563903089E-4	0.0	0.0	3.04661480328962	0.0
16	1.0669037563903089E-4	0.0	0.0	3.195021115803512	0.0
17	1.0669037563903089E-4	0.0	0.0	3.4165458990886863	0.0
18	1.0669037563903089E-4	0.0	0.0	3.663960880195599	0.0
19	1.0669037563903089E-4	0.0	0.0	3.84476550344521	0.0
20	1.0669037563903089E-4	0.0	0.0	4.018350744609913	0.0
21	1.0669037563903089E-4	0.0	0.0	4.2254367637252725	0.0
22	1.0669037563903089E-4	0.0	0.0	4.482951767059347	0.0
23	1.0669037563903089E-4	0.0	0.0	4.741213603022894	0.0
24	1.0669037563903089E-4	0.0	0.0	4.921911535896866	0.0
25	1.0669037563903089E-4	0.0	0.0	5.084436541453656	0.0
26	1.0669037563903089E-4	0.0	0.0	5.235687930651256	0.0
27	1.0669037563903089E-4	0.0	0.0	5.3988530784618805	0.0
28	1.0669037563903089E-4	0.0	0.0	5.574572127139365	0.0
29	1.0669037563903089E-4	0.0	0.0	5.786530340075572	0.0
30	1.0669037563903089E-4	0.0	0.0	6.013460769059791	0.0
31	1.0669037563903089E-4	0.0	0.0	6.224494332073794	0.0
32	1.0669037563903089E-4	0.0	0.0	6.413407423871972	0.0
33	1.0669037563903089E-4	0.0	0.0	6.607121582573905	0.0
34	1.0669037563903089E-4	0.0	0.0	6.81026005779062	0.0
35	1.0669037563903089E-4	0.0	0.0	7.040889086463658	0.0
36	1.4225383418537454E-4	0.0	0.0	7.25651033563014	0.0
37	1.4225383418537454E-4	0.0	0.0	7.466085796843743	0.0
38	1.4225383418537454E-4	0.0	0.0	7.709588797510558	0.0
39	1.4225383418537454E-4	0.0	0.0	8.027063791953767	0.0
40	1.4225383418537454E-4	0.0	0.0	8.279457657257169	0.0
41	1.4225383418537454E-4	0.0	0.0	8.519724383196266	0.0
42	1.4225383418537454E-4	0.0	0.0	8.77062458324072	0.0
43	1.4225383418537454E-4	0.0	0.0	9.02312513891976	0.0
44	1.7781729273171816E-4	0.0	0.0	9.26278728606357	0.0
45	1.7781729273171816E-4	0.0	0.0	9.512620582351634	0.0
46	1.7781729273171816E-4	0.0	0.0	9.773585241164703	0.0
47	1.7781729273171816E-4	0.0	0.0	10.042373860857968	0.0
48	1.7781729273171816E-4	0.0	0.0	10.314469882196043	0.0
49	2.1338075127806178E-4	0.0	0.0	10.593820849077574	0.0
50	2.1338075127806178E-4	0.0	0.0	10.889851078017337	0.0
51	2.1338075127806178E-4	0.0	0.0	11.17354078684152	0.0
52	2.1338075127806178E-4	0.0	0.0	11.494358746388086	0.0
53	2.1338075127806178E-4	0.0	0.0	11.785374527672817	0.0
54	2.1338075127806178E-4	0.0	0.0	12.090935763503001	0.0
55	2.1338075127806178E-4	0.0	0.0	12.394469882196043	0.0
56	2.1338075127806178E-4	0.0	0.0	12.693736385863525	0.0
57	2.1338075127806178E-4	0.0	0.0	13.013771949322072	0.0
58	2.1338075127806178E-4	0.0	0.0	13.313891975994666	0.0
59	2.1338075127806178E-4	0.0	0.0	13.630620137808402	0.0
60	2.1338075127806178E-4	0.0	0.0	13.949446543676373	0.0
61	2.4894420982440543E-4	0.0	0.0	14.275456768170704	0.0
62	2.4894420982440543E-4	0.0	0.0	14.635927983996444	0.0
63	2.4894420982440543E-4	0.0	0.0	14.99924427650589	0.0
64	2.845076683707491E-4	0.0	0.0	15.349722160480107	3.5563458546343635E-5
65	2.845076683707491E-4	0.0	0.0	15.691166925983552	3.5563458546343635E-5
66	2.845076683707491E-4	0.0	0.0	16.01507890642365	3.5563458546343635E-5
67	2.845076683707491E-4	0.0	0.0	16.349019782173816	3.5563458546343635E-5
68	2.845076683707491E-4	0.0	0.0	16.709206490331184	3.5563458546343635E-5
69	3.2007112691709267E-4	0.0	0.0	17.173523005112248	3.5563458546343635E-5
70	3.2007112691709267E-4	0.0	0.0	17.526810402311625	3.5563458546343635E-5
71	3.2007112691709267E-4	0.0	0.0	17.882765058901978	3.5563458546343635E-5
72	3.2007112691709267E-4	0.0	0.0	18.227019337630583	3.5563458546343635E-5
73	3.9119804400977997E-4	0.0	0.0	18.592256056901533	3.5563458546343635E-5
74	3.9119804400977997E-4	0.0	0.0	18.984236496999333	3.5563458546343635E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7900	0.0	69.06329	1
TAGGGAC	3545	0.0	66.00846	5
GTAAGCG	310	0.0	65.80645	1
TAAGGGA	4685	0.0	65.14407	4
CGAGGGA	1910	0.0	65.13089	4
TGCGGGC	375	0.0	65.066666	4
GACCGAT	535	0.0	65.04673	9
TCGCGCG	50	3.0013325E-10	64.0	1
TAGGGAG	3830	0.0	63.91645	5
CGGGACT	1315	0.0	63.87833	6
TACGGGA	1495	0.0	63.678932	4
ACGGGAC	1075	0.0	63.627907	5
CGAACGG	145	0.0	63.44828	2
AGGGATT	7115	0.0	63.302887	6
CTAGCGG	330	0.0	63.030304	2
ATTAGCG	315	0.0	62.222225	1
CCGAGGG	620	0.0	61.935482	3
AGCGGGA	2665	0.0	61.838646	4
TAGCGGG	2035	0.0	61.32678	3
CGACCGG	85	0.0	61.17647	2
>>END_MODULE