FastQCFastQC Report
Thu 26 May 2016
SRR1547789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547789_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4976089
Sequences flagged as poor quality0
Sequence length86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1004432.0185129325460216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT395650.075.561741
TACGCGG2650.067.924532
GCGCGAG3200.067.51
TCGATAG4650.067.096771
CGGGATC19900.066.934686
GCGCGAC1200.066.6666649
GCGGGAT43500.066.1149445
TAGGGAC61750.065.7489855
GGGCGAT29700.065.5892267
GTTAGCG4350.065.287351
CGATAGG9350.064.598932
CGTAAGG7750.064.516132
CGAGGGA42450.064.452294
TACGGGA26150.064.091784
ACGGGAC18700.063.957225
AGCGGGA49200.063.2520334
TTAGGGA91250.062.8602754
AGGGATC77200.062.5388646
TGCGGGA50800.061.5748024
AGGGATT130050.061.545566
Produced by FastQC (version 0.11.5)