##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547789_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4976089
Sequences flagged as poor quality	0
Sequence length	86
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763659773770122	31.0	31.0	33.0	30.0	34.0
2	32.20692897574782	33.0	31.0	34.0	30.0	34.0
3	32.27135567711912	33.0	31.0	34.0	30.0	34.0
4	35.914431996694596	37.0	35.0	37.0	35.0	37.0
5	35.883923699917744	37.0	35.0	37.0	35.0	37.0
6	35.94853146718236	37.0	35.0	37.0	35.0	37.0
7	36.238895244839874	37.0	35.0	37.0	35.0	37.0
8	36.20536007294082	37.0	36.0	37.0	35.0	37.0
9	38.066257054485966	39.0	39.0	39.0	37.0	39.0
10-11	37.53087163432969	39.0	37.5	39.0	35.0	39.0
12-13	37.146399009342474	39.0	37.0	39.0	33.5	39.0
14-15	38.367156917812366	40.0	38.0	41.0	33.0	41.0
16-17	38.43684216660916	40.0	37.5	41.0	34.0	41.0
18-19	38.188380372617935	40.0	37.0	41.0	34.0	41.0
20-21	37.906920475096	40.0	35.0	41.0	33.5	41.0
22-23	37.713993961924714	40.0	35.0	41.0	33.0	41.0
24-25	37.613135637244426	40.0	35.0	41.0	33.0	41.0
26-27	37.56469026176984	40.0	35.0	41.0	33.0	41.0
28-29	37.478086907207654	40.0	35.0	41.0	33.0	41.0
30-31	37.22760063174111	40.0	35.0	41.0	32.5	41.0
32-33	36.738798884023176	40.0	35.0	41.0	30.5	41.0
34-35	36.26405556653026	39.0	35.0	41.0	29.5	41.0
36-37	36.0134122400142	39.0	35.0	41.0	28.5	41.0
38-39	35.86847592557126	39.0	35.0	41.0	27.0	41.0
40-41	35.7138700694461	39.0	35.0	41.0	26.5	41.0
42-43	35.626938545512346	39.0	35.0	41.0	26.0	41.0
44-45	35.52911533535674	39.0	35.0	41.0	25.0	41.0
46-47	35.44937992467578	39.0	35.0	41.0	25.0	41.0
48-49	35.30782518158337	38.0	35.0	41.0	24.0	41.0
50-51	35.13792508534313	38.0	35.0	40.0	24.0	41.0
52-53	34.951988499401835	38.0	34.0	40.0	24.0	41.0
54-55	34.765955753604885	38.0	34.0	40.0	23.5	41.0
56-57	34.495305449721656	37.0	34.0	40.0	23.0	41.0
58-59	34.219907340885584	37.0	34.0	40.0	22.0	41.0
60-61	33.903871795701406	36.0	33.5	40.0	22.0	41.0
62-63	33.571988262267816	36.0	33.0	39.0	21.0	41.0
64-65	33.21532914704701	35.0	33.0	39.0	20.0	40.5
66-67	32.874060431796934	35.0	33.0	38.5	19.0	40.0
68-69	32.38740826379914	35.0	32.0	37.5	17.5	40.0
70-71	32.06852791419124	35.0	32.0	37.0	16.0	39.5
72-73	31.767201008663633	35.0	32.0	37.0	15.0	39.0
74-75	31.45229607026723	35.0	31.5	36.0	14.0	39.0
76-77	29.40732802407674	33.0	28.0	34.5	10.5	36.0
78-79	30.568991129378915	34.0	30.5	35.0	11.0	37.0
80-81	30.659014036927395	35.0	31.0	35.0	11.0	37.0
82-83	30.46153455052753	34.5	31.0	35.0	10.0	36.0
84-85	30.225296412503877	34.0	30.5	35.0	10.0	36.0
86	29.78818666627546	34.0	29.0	35.0	10.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	22.0
10	27.0
11	29.0
12	82.0
13	272.0
14	976.0
15	3115.0
16	7990.0
17	18378.0
18	40858.0
19	65844.0
20	73180.0
21	66985.0
22	55207.0
23	45848.0
24	43024.0
25	45576.0
26	51937.0
27	61040.0
28	73422.0
29	89894.0
30	109860.0
31	134220.0
32	161159.0
33	200381.0
34	378589.0
35	420726.0
36	446253.0
37	733888.0
38	1085574.0
39	559699.0
40	2026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.101893274014994	23.547589281461807	29.536509495710384	16.81400794881281
2	33.82988125815274	24.43822849631508	29.420454497497932	12.31143574803425
3	27.435361385216382	23.922301228937023	36.382367759097555	12.25996962674904
4	25.147982682785617	26.078291606118782	35.31026072885754	13.463464982238058
5	23.038936803582093	29.983547319993676	33.77554139405465	13.201974482369588
6	21.156916606596063	39.24634386563424	29.561710009607943	10.035029518161753
7	84.4447114993321	3.298232808938908	10.80107289077828	1.4559828009507063
8	85.29656523426328	2.904791292921007	10.38084326867948	1.4178002041362203
9	80.73456885517923	5.71235763669018	11.527366170500567	2.0257073376300143
10-11	30.33377216524865	32.44419462754786	25.867674392479717	11.354358814723772
12-13	24.706139701279458	24.3564875949767	33.86242689791119	17.07494580583265
14-15	16.59395561453985	30.607742345444382	36.014438246582806	16.783863793432953
16-17	21.650316141853573	29.169022097474546	33.25710211372827	15.923559646943614
18-19	21.251237668779638	29.699247742554448	32.944306261403284	16.105208327262634
20-21	23.547187359390076	29.001822515634267	32.94630582371015	14.504684301265511
22-23	20.488138375338544	25.455262154676088	35.602568603576024	18.454030866409344
24-25	18.90913727628264	29.489966919803884	34.81993589744878	16.780959906464695
26-27	18.22359889463392	32.97112451163956	31.951679320848157	16.85359727287836
28-29	16.71748636328651	30.079877992535902	35.81283413540232	17.389801508775264
30-31	19.760197215122158	29.30211859152841	33.242452054213665	17.695232139135776
32-33	21.187151194442063	29.612100989351276	32.04403096488025	17.156716851326415
34-35	19.02296160699698	30.0557928123874	32.016951465297346	18.904294115318276
36-37	20.523658238427807	30.662162593956822	30.985498852612963	17.828680315002405
38-39	19.139599392213444	31.576284105851	30.33321952240002	18.950896979535532
40-41	19.675301225520688	29.72660858758756	30.731664968210982	19.866425218680774
42-43	19.74316576733254	30.24994126913727	30.251759966511855	19.75513299701834
44-45	19.44356300701213	31.472638451603256	29.81358452391024	19.270214017474366
46-47	19.982962523379307	30.938564402686524	29.828566169134035	19.249906904800135
48-49	19.93065236574346	30.147772678503138	30.82257974083663	19.098995214916776
50-51	18.8304811268448	31.085888938079687	30.793159045185885	19.29047088988963
52-53	19.921076572384457	29.61173925948672	30.751027564016642	19.716156604112186
54-55	19.81904865447543	29.721966387659066	31.243261525266124	19.215723432599376
56-57	19.977405950737616	29.395434848532652	30.962297901022268	19.66486129970746
58-59	20.468886308102608	30.200344085485607	30.024834764812287	19.3059348415995
60-61	20.824587341584927	31.115962757096987	29.10691508934024	18.952534811977838
62-63	20.450779718771106	29.77116164923899	30.39794907205237	19.380109559937534
64-65	19.645669520782285	31.829414626627457	29.949303559482154	18.5756122931081
66-67	18.967807850703636	31.78449983511147	28.3628970462546	20.884795267930294
68-69	18.364100802859433	32.7223447972896	28.264024216608668	20.6495301832423
70-71	19.151677150468974	31.79985325825161	29.136667370700163	19.911802220579254
72-73	20.1107737421899	31.66283802399836	28.85755258798627	19.368835645825467
74-75	19.514411820206593	32.13312704013131	27.88772467695011	20.46473646271198
76-77	20.78258648508899	30.842515075594505	28.54668596160559	19.82821247771091
78-79	18.889945497357463	31.112556467539065	29.583484137843996	20.414013897259476
80-81	17.728983545109422	31.19903000127208	29.504848084509742	21.56713836910875
82-83	18.95376067429662	30.68625782215712	30.093312237783525	20.26666926576273
84-85	18.253441608459976	30.268299059763603	29.298169707173642	22.180089624602775
86	18.013443891377346	30.461091029521377	29.86807510878523	21.657389970316046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2687.0
1	3432.5
2	31320.0
3	34971.0
4	12103.5
5	15618.5
6	21700.0
7	24890.0
8	27234.0
9	29817.5
10	29043.5
11	27394.0
12	25970.5
13	24906.0
14	24629.0
15	24414.0
16	24168.5
17	24058.0
18	24578.0
19	25437.5
20	26756.0
21	27815.0
22	28663.0
23	31722.0
24	35897.0
25	40734.5
26	46391.5
27	53050.0
28	63128.5
29	69332.0
30	78834.5
31	93666.0
32	102022.0
33	115013.5
34	132033.5
35	142202.5
36	145316.0
37	154886.5
38	172135.0
39	183685.5
40	196974.5
41	215036.0
42	228521.0
43	233361.0
44	241678.5
45	249688.5
46	245776.0
47	239754.0
48	232068.5
49	218354.0
50	209908.0
51	185915.5
52	154905.5
53	135606.0
54	115486.0
55	97843.0
56	79789.0
57	71540.0
58	61698.5
59	46878.5
60	35527.5
61	26873.0
62	21267.5
63	14257.5
64	10571.0
65	8706.0
66	5696.0
67	3412.5
68	1951.5
69	1305.5
70	835.0
71	688.0
72	650.0
73	440.5
74	284.5
75	212.5
76	116.5
77	81.5
78	55.0
79	55.0
80	42.0
81	19.5
82	12.5
83	11.5
84	5.0
85	4.5
86	7.0
87	5.0
88	2.5
89	2.0
90	1.0
91	0.5
92	1.0
93	1.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	4976089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.10479561180516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4934594086597	14.211080394573084
2	8.044389624229115	2.9128405993678848
3	2.8302082847778047	1.5372102760421942
4	1.5665224369754636	1.1344627417099078
5	1.0392579679305558	0.9407776548661336
6	0.7733671542143068	0.8400992555960054
7	0.6088909344255585	0.7716692142359055
8	0.48129076341891	0.6970936721239228
9	0.4005505615852844	0.6526697444725813
>10	3.3110628222928487	13.37341275120402
>50	1.0646977527587604	14.000237653628709
>100	1.3757988741434226	45.02707478373902
>500	0.007566976101295576	0.9000805110368139
>1k	0.002823498545259091	0.9474779218043939
>5k	0.0	0.0
>10k+	1.1293994181036365E-4	2.053812825599386
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100443	2.0185129325460216	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0192207173143404E-5	0.0	0.0	0.037479233188956226	0.0
2	4.0192207173143404E-5	0.0	0.0	0.1080165567778229	0.0
3	4.0192207173143404E-5	0.0	0.0	0.1616530572503828	0.0
4	4.0192207173143404E-5	0.0	0.0	0.2489304351268637	0.0
5	4.0192207173143404E-5	0.0	0.0	0.4781465926353005	0.0
6	4.0192207173143404E-5	0.0	0.0	0.7448419833326936	0.0
7	4.0192207173143404E-5	0.0	0.0	0.9517514658600359	0.0
8	4.0192207173143404E-5	0.0	0.0	1.266958850615413	0.0
9	4.0192207173143404E-5	0.0	4.0192207173143404E-5	1.408756957522263	0.0
10	4.0192207173143404E-5	0.0	4.0192207173143404E-5	1.5998709026305598	0.0
11	4.0192207173143404E-5	0.0	4.0192207173143404E-5	1.8887121994803548	0.0
12	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.0820970042939346	0.0
13	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.15633201094273	0.0
14	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.191399711701298	0.0
15	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.2365154642531513	0.0
16	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.3233105356435546	0.0
17	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.4497351233066773	0.0
18	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.5890614094723787	0.0
19	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.694385088369601	0.0
20	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.8030648165657808	0.0
21	4.0192207173143404E-5	0.0	4.0192207173143404E-5	2.9326846846991685	0.0
22	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.0934133211845687	0.0
23	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.2516098486180613	0.0
24	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.3731108909024736	0.0
25	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.4776307256562333	0.0
26	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.5794375864258057	0.0
27	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.690749904191826	0.0
28	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.8124920997192775	0.0
29	4.0192207173143404E-5	0.0	4.0192207173143404E-5	3.9559179910166398	0.0
30	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.112607310681139	0.0
31	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.2546666669346145	0.0
32	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.38752200774544	0.0
33	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.523934358891089	0.0
34	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.671741200770324	0.0
35	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.82967647885719	0.0
36	4.0192207173143404E-5	0.0	4.0192207173143404E-5	4.984275803748687	0.0
37	4.0192207173143404E-5	0.0	4.0192207173143404E-5	5.143617809086614	0.0
38	4.0192207173143404E-5	0.0	4.0192207173143404E-5	5.324784182919558	0.0
39	4.0192207173143404E-5	0.0	4.0192207173143404E-5	5.568067612938595	0.0
40	4.0192207173143404E-5	0.0	4.0192207173143404E-5	5.758156656763977	0.0
41	4.0192207173143404E-5	0.0	4.0192207173143404E-5	5.948285892796531	0.0
42	4.0192207173143404E-5	0.0	4.0192207173143404E-5	6.1426955988930265	0.0
43	6.028831075971511E-5	0.0	4.0192207173143404E-5	6.332623873889715	0.0
44	6.028831075971511E-5	0.0	4.0192207173143404E-5	6.5173271619538955	0.0
45	6.028831075971511E-5	0.0	4.0192207173143404E-5	6.7129627303691715	0.0
46	6.028831075971511E-5	0.0	4.0192207173143404E-5	6.915410877900295	0.0
47	6.028831075971511E-5	0.0	4.0192207173143404E-5	7.1285300564358876	0.0
48	6.028831075971511E-5	0.0	4.0192207173143404E-5	7.340564045377806	0.0
49	6.028831075971511E-5	0.0	4.0192207173143404E-5	7.559511093953504	0.0
50	6.028831075971511E-5	0.0	4.0192207173143404E-5	7.7809500593739385	0.0
51	6.028831075971511E-5	0.0	4.0192207173143404E-5	8.008357567559584	0.0
52	6.028831075971511E-5	0.0	4.0192207173143404E-5	8.268481532384168	0.0
53	6.028831075971511E-5	0.0	4.0192207173143404E-5	8.491889915956085	0.0
54	6.028831075971511E-5	0.0	4.0192207173143404E-5	8.723316644859045	0.0
55	6.028831075971511E-5	0.0	4.0192207173143404E-5	8.958360672407588	0.0
56	1.0048051793285852E-4	0.0	4.0192207173143404E-5	9.194007583063728	0.0
57	1.0048051793285852E-4	0.0	4.0192207173143404E-5	9.43098083655658	0.0
58	1.0048051793285852E-4	0.0	4.0192207173143404E-5	9.67243552114924	0.0
59	1.0048051793285852E-4	0.0	4.0192207173143404E-5	9.914613665471016	0.0
60	1.2057662151943022E-4	0.0	4.0192207173143404E-5	10.175842915992861	0.0
61	1.4067272510600193E-4	0.0	4.0192207173143404E-5	10.438318124937075	0.0
62	1.4067272510600193E-4	0.0	4.0192207173143404E-5	10.727601536065773	0.0
63	1.4067272510600193E-4	0.0	4.0192207173143404E-5	11.020060131561152	0.0
64	1.4067272510600193E-4	0.0	4.0192207173143404E-5	11.298250493510064	0.0
65	1.4067272510600193E-4	0.0	4.0192207173143404E-5	11.558133305091609	0.0
66	1.4067272510600193E-4	0.0	4.0192207173143404E-5	11.826577056801034	0.0
67	1.4067272510600193E-4	0.0	4.0192207173143404E-5	12.091704951418674	0.0
68	1.4067272510600193E-4	0.0	4.0192207173143404E-5	12.390895741615553	0.0
69	1.4067272510600193E-4	0.0	4.0192207173143404E-5	12.751841858133968	0.0
70	1.4067272510600193E-4	0.0	4.0192207173143404E-5	13.047334965270919	0.0
71	1.6076882869257362E-4	0.0	4.0192207173143404E-5	13.346023352878134	0.0
72	1.6076882869257362E-4	0.0	4.0192207173143404E-5	13.637537431504944	0.0
73	1.8086493227914533E-4	0.0	4.0192207173143404E-5	13.936487068458783	0.0
74	2.0096103586571704E-4	0.0	4.0192207173143404E-5	14.250348014273861	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	39565	0.0	75.56174	1
TACGCGG	265	0.0	67.92453	2
GCGCGAG	320	0.0	67.5	1
TCGATAG	465	0.0	67.09677	1
CGGGATC	1990	0.0	66.93468	6
GCGCGAC	120	0.0	66.666664	9
GCGGGAT	4350	0.0	66.114944	5
TAGGGAC	6175	0.0	65.748985	5
GGGCGAT	2970	0.0	65.589226	7
GTTAGCG	435	0.0	65.28735	1
CGATAGG	935	0.0	64.59893	2
CGTAAGG	775	0.0	64.51613	2
CGAGGGA	4245	0.0	64.45229	4
TACGGGA	2615	0.0	64.09178	4
ACGGGAC	1870	0.0	63.95722	5
AGCGGGA	4920	0.0	63.252033	4
TTAGGGA	9125	0.0	62.860275	4
AGGGATC	7720	0.0	62.538864	6
TGCGGGA	5080	0.0	61.574802	4
AGGGATT	13005	0.0	61.54556	6
>>END_MODULE