FastQCFastQC Report
Thu 26 May 2016
SRR1547788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547788_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2839307
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150400.5297067206892386No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53100.18701746588163942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32070.11295009662569071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG4050.078.024692
CCGGATA650.073.8461580
TACGGGA14150.072.650184
CGTTTTT77150.071.756321
GGCGATA4450.071.011248
TCGCGCG351.18210664E-768.571431
AGGGATT54550.067.681036
ATTAGCG2650.066.41511
ACGGGAT22350.064.966445
CGATAGG6800.063.529412
TTAGCGG7900.063.2911382
CGAGGGA12650.063.2411084
ATAGGGA39800.062.9145744
TAGAGGG43300.062.8175543
GCGATAA1600.062.59
TTGCGGG16750.062.3283583
TAGCGGG17400.062.2988433
AAGTACT76300.062.28046880
GGGCGAT15550.062.250817
GCGATAT1100.061.818189
Produced by FastQC (version 0.11.5)