##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547788_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2839307
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71083683448109	31.0	31.0	33.0	30.0	34.0
2	32.153136311078725	33.0	31.0	34.0	30.0	34.0
3	32.3019979875371	34.0	31.0	34.0	30.0	34.0
4	35.89403717174648	37.0	35.0	37.0	35.0	37.0
5	35.84164692299917	37.0	35.0	37.0	35.0	37.0
6	35.901490046690974	37.0	35.0	37.0	35.0	37.0
7	36.18466900550028	37.0	35.0	37.0	35.0	37.0
8	36.139141346814554	37.0	35.0	37.0	35.0	37.0
9	38.00983373759865	39.0	39.0	39.0	35.0	39.0
10-11	37.43663629892787	39.0	37.0	39.0	34.5	39.0
12-13	36.52793850753018	39.0	35.0	39.0	33.0	39.0
14-15	37.515497619665645	40.0	35.0	41.0	33.0	41.0
16-17	37.74217493916649	40.0	35.0	41.0	33.0	41.0
18-19	37.65651653730998	39.0	36.0	41.0	33.0	41.0
20-21	37.47235839590435	39.0	35.0	41.0	33.0	41.0
22-23	37.26397656188641	39.0	35.0	41.0	33.0	41.0
24-25	37.19756440568068	39.0	35.0	41.0	33.0	41.0
26-27	37.16982700356108	39.0	35.0	41.0	33.0	41.0
28-29	37.185408270398376	39.0	35.0	41.0	33.0	41.0
30-31	37.045667129338256	39.0	35.0	41.0	32.5	41.0
32-33	36.74065291284106	39.0	35.0	41.0	31.5	41.0
34-35	36.475725062488834	39.0	35.0	41.0	31.0	41.0
36-37	36.27947136396311	38.0	35.0	41.0	30.0	41.0
38-39	36.21194449913306	38.0	35.0	41.0	30.0	41.0
40-41	36.05135003013059	38.0	35.0	41.0	30.0	41.0
42-43	36.00243545343987	38.0	35.0	41.0	30.0	41.0
44-45	35.88919549735199	38.0	35.0	41.0	30.0	41.0
46-47	35.80996472026449	38.0	35.0	40.0	30.0	41.0
48-49	35.717353037202386	38.0	35.0	40.0	29.0	41.0
50-51	35.555645268370064	37.0	35.0	40.0	29.0	41.0
52-53	35.386033810362875	37.0	34.5	40.0	28.5	41.0
54-55	35.184402391146854	37.0	34.0	40.0	28.0	41.0
56-57	34.88837399407672	36.0	34.0	40.0	27.5	41.0
58-59	34.591246032922825	36.0	34.0	40.0	26.0	41.0
60-61	34.27454727509213	35.0	34.0	39.0	26.0	41.0
62-63	33.86522028086431	35.0	33.5	39.0	25.5	40.5
64-65	33.54947281149943	35.0	33.0	38.5	25.0	40.0
66-67	33.261855445712634	35.0	33.0	38.0	24.0	40.0
68-69	32.85889673078677	35.0	33.0	37.0	22.5	40.0
70-71	32.611656118905074	35.0	33.0	36.5	22.5	39.0
72-73	32.33838204181514	35.0	32.5	36.0	21.5	39.0
74-75	32.04503634161434	35.0	32.0	36.0	20.5	38.5
76-77	29.956012858067126	33.0	29.0	34.5	18.5	36.0
78-79	31.04750067534085	34.0	30.5	35.0	19.0	37.0
80-81	31.329323493373558	34.5	31.5	35.0	19.0	36.5
82-83	31.186463457456345	34.5	31.5	35.0	19.0	36.0
84-85	30.97367368164133	34.0	31.0	35.0	18.0	36.0
86	30.577433859741127	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	10.0
11	15.0
12	48.0
13	136.0
14	399.0
15	1261.0
16	2807.0
17	5901.0
18	11690.0
19	18182.0
20	22429.0
21	23310.0
22	22111.0
23	21307.0
24	23163.0
25	26688.0
26	31898.0
27	38644.0
28	46776.0
29	57029.0
30	70544.0
31	85578.0
32	104396.0
33	137753.0
34	335924.0
35	304810.0
36	235006.0
37	391307.0
38	543809.0
39	275305.0
40	1051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.815580351120893	24.319525856133204	30.80477031895459	13.060123473791316
2	29.851439101160953	26.334630246042433	31.171761278368276	12.642169374428338
3	28.43408620483801	25.366330587005915	33.09247643879299	13.107106769363089
4	26.70581236900413	26.913292574561325	32.312708699693275	14.068186356741275
5	23.760762749501904	31.510188929904377	29.714609938270147	15.014438382323576
6	23.058866124726915	39.43321380886252	26.36928659000242	11.138633476408152
7	87.50814899551193	4.1389677128961395	6.424243662273928	1.928639629317999
8	88.2594590863193	3.439219499687776	6.169463182389224	2.131858231603698
9	83.84003561432421	5.813742578734882	7.696385068610051	2.6498367383308676
10-11	40.6381204991218	28.29396398487377	20.21507360775006	10.852841908254373
12-13	30.665017907538704	26.30110093765838	27.358682946225965	15.675198208576951
14-15	15.54148600345084	32.912608604846184	35.079739527990455	16.46616586371252
16-17	19.753394050026994	26.125459487121326	33.52973102239385	20.59141544045783
18-19	23.01385514141303	27.83254857611382	30.63108357074455	18.522512711728602
20-21	25.59897538378203	28.210985990595596	29.44343461274177	16.74660401288061
22-23	20.577609254652632	25.063739144798365	31.7443834005974	22.614268199951606
24-25	18.55778540326918	26.75165101906909	35.69957387489271	18.99098970276902
26-27	17.20009495274727	35.34961171863416	29.029495577618057	18.42079775100051
28-29	15.557704749785776	28.091115191136428	37.05501025426275	19.296169804815047
30-31	21.192935459251146	28.915506495070805	31.51300651884421	18.378551526833835
32-33	24.50877978323584	28.611206889568475	28.90883233126957	17.97118099592612
34-35	18.738428074174436	28.84483784247353	30.44334409769708	21.97338998565495
36-37	21.668368372986787	29.323739912591346	31.249579562900383	17.75831215152148
38-39	18.455577364476614	31.44388754016385	29.26182339563844	20.838711699721095
40-41	20.13711444377096	27.219987130662517	29.544128197479175	23.098770228087346
42-43	20.84498083511223	25.708139345269814	29.150651902031022	24.296227917586933
44-45	18.425429162820365	31.98572750322526	28.933556674216632	20.655286659737744
46-47	20.141287997388094	30.43769130988653	28.170465539654572	21.250555153070803
48-49	20.581430609652283	27.004441576765036	31.801333917043845	20.61279389653884
50-51	18.08295122718325	31.41095696942951	29.925823449172633	20.580268354214603
52-53	21.88692522506372	26.160644129007537	30.966253385068963	20.98617726085978
54-55	21.613759977346586	27.564560648073634	30.643005493946234	20.17867388063355
56-57	22.2661022566422	24.89517688647265	29.9853274055958	22.853393451289346
58-59	22.79094863641022	27.222012272713027	29.079049923097433	20.90798916777932
60-61	23.207282622132794	29.537277934369193	27.746893872342792	19.508545571155214
62-63	22.510739416343494	26.391140514217028	30.75190530647091	20.34621476296857
64-65	18.99477583790693	30.84900646531002	30.34700016588555	19.809217530897506
66-67	17.475743200717638	29.790896158816217	26.305380150860756	26.427980489605385
68-69	17.60223533418542	32.582052592410754	25.335865406593932	24.479846666809895
70-71	18.702380545675407	30.519700757966646	28.942115100621386	21.835803595736568
72-73	21.91742562533745	30.179600162997524	27.51197739448393	20.390996817181094
74-75	21.100747471125878	32.020172528014754	25.189738200201667	21.689341800657697
76-77	25.328469235626862	28.330803960262134	26.521348343099216	19.819378461011787
78-79	18.5767865186822	29.84934351938695	30.273672413726306	21.300197548204544
80-81	15.60111322939013	29.1713083509462	31.602324088237022	23.62525433142665
82-83	18.357208290614576	26.81886812521506	32.107429735495316	22.716493848675047
84-85	16.18095542327758	27.26605470982884	30.33143298699295	26.221556879900625
86	16.733132415762014	27.329450460975163	31.755178288223146	24.182238835039676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2652.0
1	2754.5
2	6189.0
3	6220.0
4	3434.5
5	4494.5
6	5534.5
7	6030.0
8	6369.0
9	6647.5
10	6364.5
11	6104.5
12	6037.5
13	5906.0
14	5804.0
15	5830.0
16	5937.0
17	6565.5
18	7218.5
19	7965.0
20	8277.5
21	7949.0
22	8292.5
23	9926.5
24	12379.5
25	15789.5
26	20866.5
27	26666.5
28	30285.5
29	30934.0
30	37078.0
31	48235.5
32	56611.5
33	64516.0
34	67211.0
35	67671.5
36	69979.0
37	75372.5
38	88231.5
39	96743.0
40	112317.0
41	132990.0
42	149342.5
43	159550.0
44	164133.5
45	169613.5
46	172141.5
47	173101.5
48	166873.0
49	145276.5
50	129237.0
51	109953.5
52	85363.0
53	72135.0
54	62455.5
55	60573.5
56	55301.0
57	50152.0
58	46252.5
59	36462.5
60	25969.0
61	19030.0
62	15154.0
63	10633.5
64	7653.0
65	6010.5
66	3894.0
67	3005.0
68	2018.5
69	1406.0
70	938.5
71	512.0
72	451.0
73	415.0
74	384.0
75	270.0
76	150.5
77	85.5
78	59.5
79	37.0
80	33.0
81	21.0
82	11.0
83	7.5
84	5.0
85	4.0
86	4.0
87	3.0
88	2.5
89	2.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2839307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.00786444195678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20279642378891	11.736569677101667
2	10.042511783884063	3.014333110185712
3	3.356552771413198	1.5112406695693086
4	1.6262589099342946	0.9762669307127316
5	0.8947392367411792	0.6714062587955747
6	0.5845267469370361	0.5263498908437408
7	0.4178256882842963	0.43894699030966056
8	0.31674560968277465	0.3802940138163228
9	0.2546597248792191	0.3439708766831997
>10	1.9899615451846135	6.292163879653305
>50	0.585332060327727	6.405875379745578
>100	1.6678567458021698	61.212149114561996
>500	0.05574482251436083	4.829229145728675
>1k	0.0040155168760344665	0.9398045473941694
>5k	2.36206875060851E-4	0.18823741641820524
>10k+	2.36206875060851E-4	0.5331620984801896
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15040	0.5297067206892386	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5310	0.18701746588163942	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3207	0.11295009662569071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0565958524386409E-4	0.0	0.0	0.08012518547659693	0.0
2	1.0565958524386409E-4	0.0	0.0	0.26379676449218065	0.0
3	1.0565958524386409E-4	0.0	0.0	0.37163998116441793	0.0
4	1.0565958524386409E-4	0.0	0.0	0.5632008092115435	0.0
5	1.0565958524386409E-4	0.0	0.0	0.9633336585300568	0.0
6	1.0565958524386409E-4	0.0	0.0	1.2559754897938125	0.0
7	1.0565958524386409E-4	0.0	0.0	1.4569048010658938	0.0
8	1.0565958524386409E-4	0.0	0.0	1.8411182728743316	0.0
9	1.0565958524386409E-4	0.0	0.0	1.9382194317134427	0.0
10	1.4087944699181878E-4	0.0	0.0	2.1203061169503683	0.0
11	1.4087944699181878E-4	0.0	0.0	2.450281001666956	0.0
12	1.4087944699181878E-4	0.0	0.0	2.7121054539012515	0.0
13	1.4087944699181878E-4	0.0	0.0	2.804733690298372	0.0
14	1.4087944699181878E-4	3.5219861747954695E-5	0.0	2.8448138929675446	0.0
15	1.4087944699181878E-4	3.5219861747954695E-5	0.0	2.91067503443622	0.0
16	1.4087944699181878E-4	3.5219861747954695E-5	0.0	3.0474689774652757	0.0
17	1.4087944699181878E-4	3.5219861747954695E-5	0.0	3.21391804408611	0.0
18	1.4087944699181878E-4	3.5219861747954695E-5	0.0	3.4121354260035988	0.0
19	1.4087944699181878E-4	3.5219861747954695E-5	0.0	3.5447029856229	0.0
20	1.4087944699181878E-4	3.5219861747954695E-5	0.0	3.6907245324299205	0.0
21	1.4087944699181878E-4	3.5219861747954695E-5	0.0	3.8850325096933864	0.0
22	1.4087944699181878E-4	3.5219861747954695E-5	0.0	4.084693905942542	0.0
23	1.4087944699181878E-4	3.5219861747954695E-5	0.0	4.295167799748319	0.0
24	1.4087944699181878E-4	3.5219861747954695E-5	0.0	4.450487390056799	0.0
25	1.4087944699181878E-4	3.5219861747954695E-5	0.0	4.58978194326996	0.0
26	1.4087944699181878E-4	3.5219861747954695E-5	0.0	4.723370878879952	0.0
27	1.4087944699181878E-4	3.5219861747954695E-5	0.0	4.8593195452270574	0.0
28	1.4087944699181878E-4	3.5219861747954695E-5	0.0	5.009708354890824	0.0
29	1.4087944699181878E-4	3.5219861747954695E-5	0.0	5.180665563815396	0.0
30	1.4087944699181878E-4	3.5219861747954695E-5	0.0	5.372966008959229	0.0
31	1.4087944699181878E-4	3.5219861747954695E-5	0.0	5.569387177927572	0.0
32	1.4087944699181878E-4	3.5219861747954695E-5	0.0	5.7221709381901995	0.0
33	1.4087944699181878E-4	3.5219861747954695E-5	0.0	5.884217522092539	0.0
34	1.4087944699181878E-4	3.5219861747954695E-5	0.0	6.05623132686955	0.0
35	1.4087944699181878E-4	3.5219861747954695E-5	0.0	6.253356893072852	0.0
36	1.4087944699181878E-4	3.5219861747954695E-5	0.0	6.434316542733844	0.0
37	1.4087944699181878E-4	3.5219861747954695E-5	0.0	6.623834618799587	0.0
38	1.4087944699181878E-4	3.5219861747954695E-5	0.0	6.8256796464771154	0.0
39	1.4087944699181878E-4	3.5219861747954695E-5	0.0	7.081551942076007	0.0
40	1.4087944699181878E-4	3.5219861747954695E-5	0.0	7.284594445052965	0.0
41	1.4087944699181878E-4	3.5219861747954695E-5	0.0	7.497885927798579	0.0
42	1.4087944699181878E-4	3.5219861747954695E-5	0.0	7.709240318148055	0.0
43	1.4087944699181878E-4	3.5219861747954695E-5	0.0	7.901118124950912	0.0
44	1.4087944699181878E-4	3.5219861747954695E-5	0.0	8.112190756406404	0.0
45	1.4087944699181878E-4	3.5219861747954695E-5	0.0	8.305125159061701	0.0
46	1.4087944699181878E-4	3.5219861747954695E-5	0.0	8.51073871194626	0.0
47	1.7609930873977348E-4	3.5219861747954695E-5	0.0	8.74407029602646	0.0
48	1.7609930873977348E-4	3.5219861747954695E-5	0.0	8.961095084117357	0.0
49	1.7609930873977348E-4	3.5219861747954695E-5	0.0	9.208796371790722	0.0
50	1.7609930873977348E-4	3.5219861747954695E-5	0.0	9.45216561646909	0.0
51	1.7609930873977348E-4	3.5219861747954695E-5	0.0	9.68634247723124	0.0
52	1.7609930873977348E-4	3.5219861747954695E-5	0.0	9.95721843393476	0.0
53	1.7609930873977348E-4	3.5219861747954695E-5	0.0	10.20224301211528	0.0
54	1.7609930873977348E-4	3.5219861747954695E-5	0.0	10.475337820108921	0.0
55	2.1131917048772817E-4	3.5219861747954695E-5	0.0	10.724835320731431	0.0
56	2.1131917048772817E-4	3.5219861747954695E-5	0.0	10.986694992827475	0.0
57	2.1131917048772817E-4	3.5219861747954695E-5	0.0	11.243236465799578	0.0
58	2.4653903223568287E-4	3.5219861747954695E-5	0.0	11.483259823611888	0.0
59	2.4653903223568287E-4	3.5219861747954695E-5	0.0	11.746739609348337	0.0
60	2.4653903223568287E-4	3.5219861747954695E-5	0.0	12.007683565038933	0.0
61	2.4653903223568287E-4	3.5219861747954695E-5	0.0	12.282328046949484	0.0
62	2.4653903223568287E-4	3.5219861747954695E-5	0.0	12.578632743835028	0.0
63	2.4653903223568287E-4	3.5219861747954695E-5	0.0	12.918997487767262	0.0
64	2.4653903223568287E-4	3.5219861747954695E-5	0.0	13.233158654559018	0.0
65	2.4653903223568287E-4	3.5219861747954695E-5	0.0	13.51294523628477	0.0
66	2.4653903223568287E-4	3.5219861747954695E-5	0.0	13.778679093173087	0.0
67	2.4653903223568287E-4	3.5219861747954695E-5	0.0	14.056070724299978	0.0
68	2.4653903223568287E-4	3.5219861747954695E-5	0.0	14.370795408879703	0.0
69	2.8175889398363756E-4	3.5219861747954695E-5	0.0	14.764764782392323	0.0
70	3.1697875573159226E-4	3.5219861747954695E-5	0.0	15.049763903656773	0.0
71	3.5219861747954695E-4	3.5219861747954695E-5	0.0	15.33236807432236	0.0
72	3.8741847922750165E-4	3.5219861747954695E-5	0.0	15.624869026139125	0.0
73	4.2263834097545634E-4	3.5219861747954695E-5	0.0	15.917581297126377	0.0
74	4.5785820272341104E-4	3.5219861747954695E-5	0.0	16.248049259907436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	405	0.0	78.02469	2
CCGGATA	65	0.0	73.84615	80
TACGGGA	1415	0.0	72.65018	4
CGTTTTT	7715	0.0	71.75632	1
GGCGATA	445	0.0	71.01124	8
TCGCGCG	35	1.18210664E-7	68.57143	1
AGGGATT	5455	0.0	67.68103	6
ATTAGCG	265	0.0	66.4151	1
ACGGGAT	2235	0.0	64.96644	5
CGATAGG	680	0.0	63.52941	2
TTAGCGG	790	0.0	63.291138	2
CGAGGGA	1265	0.0	63.241108	4
ATAGGGA	3980	0.0	62.914574	4
TAGAGGG	4330	0.0	62.817554	3
GCGATAA	160	0.0	62.5	9
TTGCGGG	1675	0.0	62.328358	3
TAGCGGG	1740	0.0	62.298843	3
AAGTACT	7630	0.0	62.280468	80
GGGCGAT	1555	0.0	62.25081	7
GCGATAT	110	0.0	61.81818	9
>>END_MODULE