FastQCFastQC Report
Thu 26 May 2016
SRR1547787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547787_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3400057
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173220.5094620472539136No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC57920.17035008530739337TruSeq Adapter, Index 10 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47610.1400270642521581No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44090.12967429663679167No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG42460.12488025936035778RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG37590.11055697007432523RNA PCR Primer, Index 35 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG1200.073.333332
CGTTTTT89250.070.364141
TATAGCG2500.068.81
TACGGGA13750.068.363644
TAAGGGA45500.067.076924
TAACGGG12350.066.0728763
CGGGATA10300.066.0194246
GCGAACG1350.065.185191
ACGGGAC11850.065.147685
TAGGGAG39650.064.1614155
AGTAGGG60950.064.1181263
ACGGGTA4250.064.05
TATGGGA36600.062.2950864
CTAGACG1100.061.818181
CTACGGG4400.061.818183
GTAGGGA57650.061.5437974
AGGGATT54150.061.5327766
ATAGCGG6000.061.3333322
CGGGACT14950.061.2709056
ACGGGAT15950.061.191225
Produced by FastQC (version 0.11.5)