##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547787_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3400057
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65500313671212	31.0	31.0	33.0	30.0	34.0
2	32.0760722540828	33.0	31.0	34.0	30.0	34.0
3	32.241990943093015	33.0	31.0	34.0	30.0	34.0
4	35.86000911161195	37.0	35.0	37.0	35.0	37.0
5	35.785456832047224	37.0	35.0	37.0	35.0	37.0
6	35.842174410605466	37.0	35.0	37.0	35.0	37.0
7	36.13984324380444	37.0	35.0	37.0	35.0	37.0
8	36.07909014466522	37.0	35.0	37.0	35.0	37.0
9	37.916182581644954	39.0	38.0	39.0	35.0	39.0
10-11	37.376562069400606	39.0	37.0	39.0	34.5	39.0
12-13	36.51032688569633	39.0	35.0	39.0	33.0	39.0
14-15	37.48566685793797	40.0	35.0	41.0	33.0	41.0
16-17	37.67151138936788	40.0	35.0	41.0	33.0	41.0
18-19	37.59642323643398	39.0	35.5	41.0	33.0	41.0
20-21	37.409276373896084	39.0	35.0	41.0	33.0	41.0
22-23	37.192409421371465	39.0	35.0	41.0	32.5	41.0
24-25	37.098092031986525	39.0	35.0	41.0	32.0	41.0
26-27	37.06882046389222	39.0	35.0	41.0	32.0	41.0
28-29	37.05504040079328	39.0	35.0	41.0	32.0	41.0
30-31	36.919295764747474	39.0	35.0	41.0	32.0	41.0
32-33	36.64598196442001	39.0	35.0	41.0	31.0	41.0
34-35	36.40255722183481	38.0	35.0	41.0	30.5	41.0
36-37	36.245952494325834	38.0	35.0	41.0	30.0	41.0
38-39	36.16197272575137	38.0	35.0	41.0	30.0	41.0
40-41	35.980102392401065	38.0	35.0	41.0	30.0	41.0
42-43	35.9497886358964	38.0	35.0	41.0	30.0	41.0
44-45	35.842949250556686	38.0	35.0	40.5	30.0	41.0
46-47	35.74250490506483	38.0	35.0	40.0	29.0	41.0
48-49	35.66806174131787	38.0	35.0	40.0	29.0	41.0
50-51	35.48861357324304	37.0	35.0	40.0	29.0	41.0
52-53	35.29505564171424	37.0	34.0	40.0	28.0	41.0
54-55	35.08223273903938	36.5	34.0	40.0	28.0	41.0
56-57	34.767281107346136	36.0	34.0	40.0	26.5	41.0
58-59	34.39547381117434	36.0	34.0	39.5	26.0	41.0
60-61	34.037528782605705	35.0	33.5	39.0	25.5	41.0
62-63	33.554893638547824	35.0	33.0	39.0	23.5	40.5
64-65	33.15275596850288	35.0	32.5	38.0	22.5	40.0
66-67	32.80593987100805	35.0	32.5	37.5	21.0	40.0
68-69	32.326583789624706	35.0	32.0	37.0	19.5	39.5
70-71	32.005024768702405	35.0	32.0	36.5	18.5	39.0
72-73	31.710664556505964	35.0	31.5	36.0	18.0	39.0
74-75	31.384921193968218	35.0	31.0	35.5	17.5	38.0
76-77	29.369270279880602	32.5	28.0	34.5	12.5	36.0
78-79	30.380011276281543	34.0	30.0	35.0	13.0	36.5
80-81	30.593948424982287	34.0	31.0	35.0	12.5	36.0
82-83	30.404348515333716	34.0	31.0	35.0	11.0	36.0
84-85	30.167436604739272	34.0	30.0	35.0	10.0	36.0
86	29.76243045337181	34.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	20.0
10	26.0
11	34.0
12	53.0
13	173.0
14	503.0
15	1566.0
16	3699.0
17	7541.0
18	14369.0
19	21403.0
20	26249.0
21	27664.0
22	26658.0
23	27277.0
24	30713.0
25	36579.0
26	44258.0
27	54090.0
28	65544.0
29	80915.0
30	99003.0
31	117230.0
32	137752.0
33	172215.0
34	396349.0
35	353409.0
36	276064.0
37	464831.0
38	613731.0
39	299122.0
40	1011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18076726360764	23.44919511643481	29.994350094719003	13.375687525238547
2	29.419918548424334	25.7340097533659	31.371297598834374	13.474774099375392
3	28.015147981342665	25.45224977110678	32.16775483469836	14.364847412852196
4	26.757022014630934	26.544613810886116	30.911040609025086	15.787323565457873
5	22.882086976777156	31.90067107698489	29.59738616146729	15.619855784770667
6	22.58288611043874	38.45338475207916	27.566067274754513	11.397661862727595
7	85.95520604507513	4.316251168730407	7.20908502416283	2.5194577620316365
8	85.6377996015949	3.8042597521159203	7.474286460491691	3.0836541857974735
9	80.49717990021932	6.588860127933149	9.14405258500078	3.76990738684675
10-11	39.55293984777314	27.41752564736415	20.53127344629811	12.498261058564607
12-13	29.999158837631253	26.00959336858176	27.28001324683674	16.711234546950244
14-15	15.653928742959309	32.35088411753097	34.61418440926137	17.381002730248348
16-17	19.990797213105544	26.17726702817041	32.85053750569476	20.981398253029287
18-19	23.19368469410954	28.308275420088545	29.624062184839843	18.873977700962072
20-21	25.76646509161464	28.25627629183864	28.553256607168642	17.42400200937808
22-23	21.053249989632526	25.508881174639132	30.045349239733333	23.392519595995008
24-25	20.006649888516577	26.803124182918108	33.48871798325734	19.701507945307974
26-27	17.572705398762434	33.20639918683716	29.066159773203804	20.154735641196602
28-29	16.092553742481375	28.140204708332835	35.258320669329954	20.50892087985584
30-31	21.377435731224505	28.223173905613937	30.617869053371756	19.781521309789806
32-33	24.36326802756542	27.16114759252565	29.070968516116054	19.40461586379287
34-35	18.271605446614572	27.118868889550967	31.443369919974874	23.166155743859587
36-37	21.61087299418804	28.70747460998448	30.102862981414724	19.57878941441276
38-39	19.023710484853638	30.70714696841847	29.14993778045486	21.119204766273036
40-41	20.075678143042897	26.144458754662054	29.48031459472591	24.29954850756914
42-43	20.378805414144527	25.428897221428937	28.438758526695292	25.75353883773125
44-45	18.747744523106526	30.036143511711717	28.40937666633236	22.8067352988494
46-47	19.861269972826925	28.935103146800184	28.871501271890445	22.332125608482446
48-49	20.04060520161868	25.105461467263634	32.67329047718906	22.180642853928624
50-51	18.467263931163508	29.00076381072435	29.726236942498318	22.805735315613827
52-53	21.03591204500395	24.885494566708736	31.391694315712943	22.68689907257437
54-55	20.639698099178926	26.1235620461657	32.02400724458443	21.21273261007095
56-57	21.60010846876979	25.02312461232268	29.89898404644393	23.47778287246361
58-59	22.913557037426138	27.502333049122413	28.60490280015894	20.97920711329251
60-61	22.765324228387936	29.557725061668084	27.080428357524593	20.596522352419385
62-63	21.584858724427267	27.008753088551163	30.74635219350734	20.660035993514224
64-65	18.448984825842626	30.96240739493485	30.43271039279636	20.15589738642617
66-67	18.18676863358467	29.507431787172976	26.366131508971762	25.93966807027059
68-69	17.43626650964969	31.811407867573987	26.245383533276062	24.506942089500264
70-71	17.762628685342627	30.42512228471464	29.63756195852011	22.17468707142263
72-73	20.698211824095893	30.747249237292195	27.597919681934748	20.95661925667717
74-75	19.951739044374843	31.947023241080956	25.84209617662292	22.259141537921277
76-77	23.71430831894877	28.241055958767753	27.144280228243233	20.900355494040245
78-79	17.793951689633438	28.97467601278449	30.529282303208443	22.702089994373623
80-81	16.202243080042482	28.763458965540874	30.647500909543574	24.38679704487307
82-83	17.4157668533204	28.162145516972213	30.787910320326983	23.6341773093804
84-85	16.14884691638993	27.628904456601756	29.27984736726473	26.942401259743587
86	17.466765998334736	27.07645783585393	31.028597461748436	24.4281787040629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3509.0
1	3709.5
2	7675.0
3	7475.5
4	4130.0
5	5481.5
6	6643.5
7	7073.0
8	7048.0
9	7155.0
10	6977.0
11	6405.5
12	6278.5
13	6454.0
14	6495.0
15	6403.5
16	6503.5
17	6994.0
18	7768.0
19	8401.5
20	9197.5
21	9835.0
22	9745.0
23	10821.0
24	13333.5
25	15315.5
26	17414.5
27	23734.5
28	34562.0
29	40533.0
30	42696.0
31	49607.0
32	56560.0
33	63182.5
34	71472.0
35	79681.0
36	84018.0
37	87636.5
38	101383.5
39	116738.0
40	133754.0
41	157291.5
42	179169.0
43	189299.0
44	194623.5
45	197733.0
46	204852.5
47	213084.0
48	202634.0
49	175974.0
50	158661.0
51	139027.0
52	112546.0
53	98219.5
54	89777.0
55	85512.5
56	75360.5
57	68510.0
58	61449.0
59	44293.5
60	30375.5
61	24160.0
62	19525.0
63	15463.5
64	13645.0
65	10583.5
66	6315.0
67	4183.0
68	2880.0
69	2046.5
70	1186.5
71	782.0
72	725.5
73	436.5
74	159.5
75	91.0
76	61.5
77	78.0
78	67.0
79	34.0
80	26.5
81	14.5
82	12.5
83	9.0
84	3.0
85	2.5
86	2.0
87	2.5
88	3.0
89	2.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3400057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.311455289000111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76602816153178	11.60088152620417
2	10.743328480864443	3.2899198737959474
3	3.9089522442017475	1.7955524254179511
4	2.0103809686504546	1.2312743326139266
5	1.1545382699075946	0.8838830549564839
6	0.7505519444112317	0.6895225523354803
7	0.5302567479819627	0.5683301741891494
8	0.40829985946351105	0.5001332034144462
9	0.3085999395478429	0.42526027589174403
>10	2.223622748466311	6.939454873742537
>50	0.548354198672901	6.1885765968465725
>100	1.590250224434247	59.082577424920935
>500	0.051404764998246866	4.652009621373489
>1k	0.005043486377186485	1.4661105615905188
>5k	1.9398024527640326E-4	0.17202934185673763
>10k+	1.9398024527640326E-4	0.5144841608498635
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17322	0.5094620472539136	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5792	0.17035008530739337	TruSeq Adapter, Index 10 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4761	0.1400270642521581	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4409	0.12967429663679167	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	4246	0.12488025936035778	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	3759	0.11055697007432523	RNA PCR Primer, Index 35 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18208518268958432	0.0
2	0.0	0.0	0.0	0.5773726734581215	0.0
3	0.0	0.0	0.0	0.8048688595514722	0.0
4	0.0	0.0	0.0	1.1780390740508173	0.0
5	0.0	0.0	0.0	1.9318205547730523	0.0
6	0.0	0.0	0.0	2.5079285435508876	0.0
7	0.0	0.0	0.0	2.8629225921800723	0.0
8	0.0	0.0	0.0	3.5439994094216654	0.0
9	0.0	0.0	0.0	3.722231715527122	0.0
10	0.0	0.0	0.0	4.09013731240388	0.0
11	0.0	0.0	0.0	4.654951372874043	0.0
12	0.0	0.0	0.0	5.108443770207382	0.0
13	0.0	0.0	0.0	5.26967636130806	0.0
14	0.0	0.0	0.0	5.34082222739207	0.0
15	0.0	0.0	0.0	5.455379130408696	0.0
16	0.0	0.0	0.0	5.695345695675102	0.0
17	0.0	0.0	0.0	5.976664508859704	0.0
18	0.0	0.0	0.0	6.326452762409572	0.0
19	2.9411271634563773E-5	0.0	0.0	6.54503733319765	0.0
20	2.9411271634563773E-5	0.0	0.0	6.776356984603494	0.0
21	2.9411271634563773E-5	0.0	0.0	7.075234326953931	0.0
22	2.9411271634563773E-5	0.0	0.0	7.3637589016890015	0.0
23	2.9411271634563773E-5	0.0	0.0	7.673489003272592	0.0
24	2.9411271634563773E-5	0.0	0.0	7.900573431563059	0.0
25	2.9411271634563773E-5	0.0	0.0	8.094834880709353	0.0
26	2.9411271634563773E-5	0.0	0.0	8.289743377831607	0.0
27	2.9411271634563773E-5	0.0	0.0	8.491739991417791	0.0
28	5.882254326912755E-5	0.0	0.0	8.697030667427047	0.0
29	5.882254326912755E-5	0.0	0.0	8.943967704070843	0.0
30	5.882254326912755E-5	0.0	0.0	9.213051428255467	0.0
31	8.823381490369133E-5	0.0	0.0	9.45963552963965	0.0
32	8.823381490369133E-5	0.0	0.0	9.677102472105615	0.0
33	8.823381490369133E-5	0.0	0.0	9.896216445783114	0.0
34	8.823381490369133E-5	0.0	0.0	10.120095045465414	0.0
35	8.823381490369133E-5	0.0	0.0	10.37797307515727	0.0
36	8.823381490369133E-5	0.0	0.0	10.611351515577534	0.0
37	8.823381490369133E-5	0.0	0.0	10.847759316976157	0.0
38	8.823381490369133E-5	0.0	0.0	11.091814048999767	0.0
39	8.823381490369133E-5	0.0	0.0	11.424220241013607	0.0
40	1.176450865382551E-4	0.0	0.0	11.679627723888158	0.0
41	1.176450865382551E-4	0.0	0.0	11.951711397779508	0.0
42	1.176450865382551E-4	0.0	0.0	12.227942060971332	0.0
43	1.176450865382551E-4	0.0	0.0	12.47173209155023	0.0
44	1.4705635817281886E-4	0.0	0.0	12.712492761150768	0.0
45	1.4705635817281886E-4	0.0	0.0	12.956547493174378	0.0
46	1.4705635817281886E-4	0.0	0.0	13.225248870827754	0.0
47	1.4705635817281886E-4	0.0	0.0	13.502332460896978	0.0
48	1.4705635817281886E-4	0.0	0.0	13.76715155069459	0.0
49	1.4705635817281886E-4	0.0	0.0	14.038735232968154	0.0
50	1.4705635817281886E-4	0.0	0.0	14.325465720133515	0.0
51	1.4705635817281886E-4	0.0	0.0	14.600049352113803	0.0
52	1.7646762980738265E-4	0.0	0.0	14.914397023344021	0.0
53	1.7646762980738265E-4	0.0	0.0	15.190451218906036	0.0
54	1.7646762980738265E-4	0.0	0.0	15.487857997674745	0.0
55	1.7646762980738265E-4	0.0	0.0	15.776706096397795	0.0
56	1.7646762980738265E-4	0.0	0.0	16.06576007402229	0.0
57	2.0587890144194642E-4	0.0	0.0	16.365343286891957	0.0
58	2.0587890144194642E-4	0.0	0.0	16.630221199232835	0.0
59	2.0587890144194642E-4	0.0	0.0	16.907863603463117	0.0
60	2.0587890144194642E-4	0.0	0.0	17.21385847354912	0.0
61	2.0587890144194642E-4	0.0	0.0	17.516265168495703	0.0
62	2.0587890144194642E-4	0.0	0.0	17.85605358968982	0.0
63	2.352901730765102E-4	0.0	0.0	18.219459262006488	0.0
64	2.352901730765102E-4	0.0	0.0	18.54027741299631	0.0
65	2.941127163456377E-4	0.0	0.0	18.854389794053453	0.0
66	2.941127163456377E-4	0.0	0.0	19.138532089314975	0.0
67	2.941127163456377E-4	0.0	0.0	19.43670356114618	0.0
68	3.529352596147653E-4	0.0	0.0	19.79152114214556	0.0
69	3.823465312493291E-4	0.0	0.0	20.229337331697675	0.0
70	4.1175780288389284E-4	0.0	0.0	20.537067466810115	0.0
71	4.411690745184566E-4	0.0	0.0	20.83762125164372	0.0
72	4.411690745184566E-4	0.0	0.0	21.142410259592708	0.0
73	4.411690745184566E-4	0.0	0.0	21.445316946157078	0.0
74	4.705803461530204E-4	0.0	0.0	21.791105266764646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	120	0.0	73.33333	2
CGTTTTT	8925	0.0	70.36414	1
TATAGCG	250	0.0	68.8	1
TACGGGA	1375	0.0	68.36364	4
TAAGGGA	4550	0.0	67.07692	4
TAACGGG	1235	0.0	66.072876	3
CGGGATA	1030	0.0	66.019424	6
GCGAACG	135	0.0	65.18519	1
ACGGGAC	1185	0.0	65.14768	5
TAGGGAG	3965	0.0	64.161415	5
AGTAGGG	6095	0.0	64.118126	3
ACGGGTA	425	0.0	64.0	5
TATGGGA	3660	0.0	62.295086	4
CTAGACG	110	0.0	61.81818	1
CTACGGG	440	0.0	61.81818	3
GTAGGGA	5765	0.0	61.543797	4
AGGGATT	5415	0.0	61.532776	6
ATAGCGG	600	0.0	61.333332	2
CGGGACT	1495	0.0	61.270905	6
ACGGGAT	1595	0.0	61.19122	5
>>END_MODULE