FastQCFastQC Report
Thu 26 May 2016
SRR1547786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547786_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2250970
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131560.5844591442800215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74150.071.2609561
TACGGGT3900.067.692314
CGAGGGT3500.065.142864
GGGCGAT13700.064.525547
TACGCGG2500.064.02
TTGCGGG19550.063.0179063
CCGATCC13300.062.85714380
TAGGGCG3150.062.2222255
AGGGATC28050.062.1746866
TAAGGGA30100.062.05984
CGAGGGA14300.061.258744
CGGGATA7450.061.208056
TAGGGAC23700.061.0970465
TAGGGAT39450.060.9378975
ATAGGGA34350.060.6695824
TCGATAG1650.060.606061
CGGGTAT3800.060.06
ATTAGCG2600.059.9999961
TACGGGA8650.059.6531754
AGGGATT48200.059.33616