FastQCFastQC Report
Thu 26 May 2016
SRR1547785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547785_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1903775
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94930.4986408582947039No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28700.15075310895457708No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27340.14360940762432536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT500.080.01
CGGGATA5200.075.384626
CTAGCGG3050.073.442632
TCGAACG600.073.3333361
TATAGCG1750.073.142861
CGAGGGA10950.072.694074
CGTTTTT51000.072.47061
CGTTAGG2850.070.175442
CGAACGG1450.068.9655152
ACGAGGG8250.068.848493
CGGGGTA2500.068.86
CGGGTCC1050.068.571436
ATAGCGG3500.068.571432
TACGGGA5200.068.461544
TTGCGAG1650.067.8787841
TTAGGCG600.066.666671
CGGGATC6300.066.6666646
TAGCGGG13250.065.811323
TAGGGTG5600.065.714295
CGTAAGG4700.065.531912
Produced by FastQC (version 0.11.5)