##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547785_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1903775
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76971070635973	31.0	31.0	33.0	30.0	34.0
2	32.21341282451971	33.0	31.0	34.0	31.0	34.0
3	32.36754238289714	34.0	31.0	34.0	30.0	34.0
4	35.963799818781105	37.0	35.0	37.0	35.0	37.0
5	35.902853540990925	37.0	35.0	37.0	35.0	37.0
6	35.96131948365747	37.0	35.0	37.0	35.0	37.0
7	36.20816167876981	37.0	35.0	37.0	35.0	37.0
8	36.16699452403777	37.0	35.0	37.0	35.0	37.0
9	38.06512849470132	39.0	39.0	39.0	37.0	39.0
10-11	37.523333114469935	39.0	37.5	39.0	35.0	39.0
12-13	36.47263489645573	39.0	35.0	39.0	33.0	39.0
14-15	37.372216254546885	40.0	35.0	41.0	33.0	41.0
16-17	37.64046460322255	40.0	35.0	41.0	33.0	41.0
18-19	37.53775104726137	39.0	35.0	41.0	33.0	41.0
20-21	37.34504471379233	39.0	35.0	41.0	33.0	41.0
22-23	37.18024766582185	39.0	35.0	41.0	33.0	41.0
24-25	37.112185788761806	38.5	35.0	41.0	33.0	41.0
26-27	37.08283830809838	39.0	35.0	41.0	33.0	41.0
28-29	37.11478325957637	39.0	35.0	41.0	33.0	41.0
30-31	36.95802602723536	38.0	35.0	41.0	32.5	41.0
32-33	36.655206366298536	38.0	35.0	41.0	31.5	41.0
34-35	36.3984845898281	38.0	35.0	41.0	31.0	41.0
36-37	36.21571535501832	38.0	35.0	41.0	30.5	41.0
38-39	36.128538036270044	38.0	35.0	41.0	30.0	41.0
40-41	35.956004779976624	38.0	35.0	41.0	30.0	41.0
42-43	35.929915299864746	38.0	35.0	41.0	30.0	41.0
44-45	35.824190358629565	38.0	35.0	40.0	30.0	41.0
46-47	35.770828748145135	37.5	35.0	40.0	30.0	41.0
48-49	35.70651600110307	37.0	35.0	40.0	30.0	41.0
50-51	35.50307021575554	37.0	35.0	40.0	29.0	41.0
52-53	35.32479520951793	36.5	34.5	40.0	29.0	41.0
54-55	35.1404154902759	36.0	34.0	40.0	29.0	41.0
56-57	34.829035994274534	36.0	34.0	40.0	27.5	41.0
58-59	34.53993040143925	35.5	34.0	39.0	27.0	41.0
60-61	34.24113668894696	35.0	34.0	39.0	26.5	41.0
62-63	33.818662657089206	35.0	33.5	39.0	26.0	40.0
64-65	33.51978096151068	35.0	33.0	38.0	25.0	40.0
66-67	33.29757560636105	35.0	33.0	37.0	25.0	40.0
68-69	32.92983598376909	35.0	33.0	37.0	24.0	39.5
70-71	32.712471799451095	35.0	33.0	36.0	24.0	39.0
72-73	32.456771414689236	35.0	33.0	36.0	23.5	39.0
74-75	32.17157909942089	35.0	32.5	35.5	23.0	37.5
76-77	30.13525915615028	33.0	29.5	34.5	19.5	36.0
78-79	31.19829339076309	34.0	31.0	35.0	20.0	36.0
80-81	31.500949692059198	35.0	32.0	35.0	20.0	36.0
82-83	31.373736917440347	35.0	32.0	35.0	20.0	36.0
84-85	31.19586873448806	34.0	32.0	35.0	19.0	36.0
86	30.79808065553965	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	11.0
10	27.0
11	24.0
12	43.0
13	102.0
14	272.0
15	822.0
16	1965.0
17	4254.0
18	8317.0
19	12179.0
20	14530.0
21	15160.0
22	14011.0
23	12992.0
24	13630.0
25	16060.0
26	19444.0
27	23829.0
28	29432.0
29	36219.0
30	45351.0
31	55915.0
32	70687.0
33	97030.0
34	251277.0
35	217404.0
36	154929.0
37	272509.0
38	350023.0
39	164900.0
40	419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.663300547596222	23.773765282136807	31.437853737967984	13.125080432298986
2	30.23224908405668	25.609854105658492	30.828222872976063	13.329673937308767
3	29.413927591233207	24.522278105343332	32.772307651902146	13.29148665152132
4	27.470525666110753	26.137069769274206	32.32083623327336	14.071568331341677
5	24.635054037373113	31.118435739517537	30.027603051831232	14.21890717127812
6	22.3397722945201	40.69630077083689	26.544838544470856	10.419088390172158
7	88.11487702065632	3.943533243161613	6.27295767619598	1.6686320599860804
8	89.53873225564996	3.1125526913632124	5.60092447899568	1.7477905739911492
9	85.27005554753056	5.573610326850599	6.645112999172696	2.51122112644614
10-11	43.53747160247403	26.034983125631967	18.564851413638692	11.862693858255309
12-13	32.54730732360704	26.77535423041063	24.67148166143583	16.005856784546495
14-15	15.659860014970256	32.776457301939566	35.01101758348544	16.55266509960473
16-17	21.6562619006973	24.897243634358052	32.62809418129768	20.818400283646966
18-19	25.5156465443658	27.330330527504564	29.189872752820058	17.96415017530958
20-21	26.893382884006776	28.28879463171856	27.866712190253573	16.951110294021092
22-23	21.01968457406994	24.379325287914803	31.993722997728195	22.60726714028706
24-25	20.574962902653937	26.608764165933472	34.1204711691245	18.695801762288085
26-27	17.495843784060618	34.4831978568896	28.970361518561806	19.050596840487977
28-29	15.935444051949416	29.515882916836283	35.77770482331158	18.77096820790272
30-31	23.457577707449673	28.778899817467924	30.409896127431026	17.353626347651378
32-33	27.301072868379926	27.80328557733976	27.766463999159562	17.129177555120748
34-35	19.02225840763746	29.25498023663511	29.824375254428702	21.898386101298737
36-37	23.656734645638274	30.046171422568317	29.121561108849527	17.175532822943886
38-39	20.064450893619256	31.254507491694135	27.583905661120667	21.09713595356594
40-41	21.012540872739688	26.338354059697185	28.609893501070243	24.039211566492888
42-43	21.479586610812728	25.817310343921946	28.341243056558678	24.361859988706648
44-45	18.67741723680582	31.850113590103874	28.090425601764913	21.382043571325394
46-47	21.087864900001314	29.487702065632753	28.573308295360533	20.851124739005396
48-49	22.039342884532047	26.245039461070768	30.730390933802575	20.985226720594607
50-51	19.076676603064964	31.813685966041156	27.867342516841536	21.242294914052344
52-53	22.584496592296883	26.189649512153483	29.341256188362596	21.884597707187037
54-55	22.315005712334703	27.5551995377605	30.918648474740973	19.211146275163816
56-57	22.909088521490197	25.255873199301387	28.971674698953397	22.86336358025502
58-59	24.121364131790784	27.509894814250636	27.69276831558351	20.67597273837507
60-61	25.153051174639863	29.28539349450434	25.877348951425454	19.684206379430343
62-63	23.936547123478352	25.464406245485943	30.550747856233013	20.048298774802696
64-65	20.31521582119736	30.23794828695618	30.309647936337015	19.13718795550945
66-67	18.583419784375778	29.63519848721619	25.75995062441728	26.021431103990757
68-69	17.695132040288374	32.581134850494415	24.93222675999002	24.791506349227195
70-71	18.884216884873474	30.442909482475606	28.939317934104608	21.733555698546308
72-73	22.754317080537355	30.20627437591102	26.76266890782787	20.276739635723757
74-75	21.42992212840278	32.133550445824746	24.185316016861236	22.251211408911242
76-77	26.449948129374533	27.868051634253	25.637641002744548	20.044359233627922
78-79	19.103885700778715	29.380914236188627	29.550944833291748	21.96425522974091
80-81	17.029218263712885	28.784310120681276	30.90480755341361	23.281664062192224
82-83	18.879830862365562	27.17211855392575	31.612559257265172	22.335491326443513
84-85	16.66507334112487	27.044267311000514	30.030465785084896	26.26019356278972
86	17.38561542199052	26.832477577444813	31.820199340783446	23.961707659781222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2092.0
1	1916.5
2	3987.5
3	4112.0
4	2396.5
5	3266.0
6	3980.0
7	4231.0
8	4449.5
9	4560.5
10	4173.0
11	3860.0
12	3947.5
13	4093.0
14	4118.0
15	4277.0
16	4302.0
17	4187.0
18	4385.0
19	4727.0
20	4772.5
21	4655.0
22	5166.5
23	6014.5
24	6590.5
25	8783.5
26	11809.0
27	13357.0
28	14712.5
29	15592.0
30	20788.5
31	25744.0
32	26547.5
33	33156.5
34	41693.0
35	45232.0
36	45799.0
37	45463.5
38	47589.0
39	58014.5
40	72938.0
41	88585.0
42	97219.5
43	97166.0
44	105382.5
45	112252.5
46	110926.5
47	115257.5
48	112888.5
49	99277.0
50	92345.0
51	81058.5
52	65518.0
53	58827.5
54	54219.0
55	49512.5
56	45293.0
57	43608.0
58	39454.5
59	31706.5
60	23305.5
61	16612.5
62	13302.5
63	9345.5
64	6812.0
65	6187.5
66	4240.5
67	2369.5
68	1598.0
69	1235.0
70	865.0
71	635.0
72	484.5
73	253.0
74	175.5
75	137.5
76	71.5
77	37.5
78	49.0
79	70.0
80	44.0
81	11.0
82	7.5
83	10.0
84	6.0
85	3.0
86	3.0
87	3.0
88	2.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1903775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.182684794646123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24725517544377	12.031860961696513
2	9.31856411269191	2.8296164332340497
3	3.008683731444888	1.3703969022392237
4	1.416707276787244	0.8603768011896882
5	0.7799311939591492	0.5920724739696885
6	0.5333731168504701	0.4858821546647185
7	0.3693278502717209	0.39251718355918574
8	0.3151510677674152	0.382787145968706
9	0.25969903332591243	0.35486357080154646
>10	2.1488788281310813	7.15052903462528
>50	0.7459412383465719	8.326437794848534
>100	1.8317388575891154	61.93496217452578
>500	0.02126281071606774	1.9919456764746886
>1k	0.003137136007288683	0.7933594844717463
>5k	3.485706674765203E-4	0.5023922077306575
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9493	0.4986408582947039	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2870	0.15075310895457708	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2734	0.14360940762432536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08173234757258604	0.0
2	0.0	0.0	0.0	0.22560439127522947	0.0
3	0.0	0.0	0.0	0.31353495029612216	0.0
4	0.0	0.0	0.0	0.42914735197174037	0.0
5	0.0	0.0	0.0	0.6637864243411118	0.0
6	0.0	0.0	0.0	0.8507307848879201	0.0
7	0.0	0.0	0.0	0.9661856049165474	0.0
8	0.0	0.0	0.0	1.189321217055587	0.0
9	0.0	0.0	0.0	1.265590734199157	0.0
10	0.0	0.0	0.0	1.412246720331972	0.0
11	0.0	0.0	0.0	1.7352890966632086	0.0
12	0.0	0.0	0.0	1.9634673215059553	0.0
13	0.0	0.0	0.0	2.0520807343304748	0.0
14	0.0	0.0	0.0	2.0918438365878322	0.0
15	0.0	0.0	0.0	2.1585008732649604	0.0
16	0.0	0.0	0.0	2.284986408582947	0.0
17	0.0	0.0	0.0	2.4445640897690115	0.0
18	0.0	0.0	0.0	2.6177463198119524	0.0
19	0.0	0.0	0.0	2.739609460151541	0.0
20	0.0	0.0	0.0	2.870402227153944	0.0
21	0.0	0.0	0.0	3.0268282754001916	0.0
22	0.0	0.0	0.0	3.1865110110175836	0.0
23	0.0	0.0	0.0	3.340363225696314	0.0
24	0.0	0.0	0.0	3.463749655290147	0.0
25	0.0	0.0	0.0	3.5762104240259482	0.0
26	0.0	0.0	0.0	3.6954997307980197	0.0
27	0.0	0.0	0.0	3.8611705690010636	0.0
28	0.0	0.0	0.0	3.9918582815721395	0.0
29	0.0	0.0	0.0	4.150122782366614	0.0
30	0.0	0.0	0.0	4.318104818058857	0.0
31	0.0	0.0	0.0	4.46922561752308	0.0
32	0.0	0.0	0.0	4.605060997229189	0.0
33	0.0	0.0	0.0	4.738900342740082	0.0
34	0.0	0.0	0.0	4.900841748631009	0.0
35	0.0	0.0	0.0	5.082480860395792	0.0
36	0.0	0.0	0.0	5.231710680096125	0.0
37	0.0	0.0	0.0	5.412982101351263	0.0
38	0.0	0.0	0.0	5.616367480400783	0.0
39	0.0	0.0	0.0	6.002862733253667	0.0
40	0.0	0.0	0.0	6.214179721868393	0.0
41	0.0	0.0	0.0	6.413573032527478	0.0
42	0.0	0.0	0.0	6.630562960433875	0.0
43	0.0	0.0	0.0	6.794605454951347	0.0
44	0.0	0.0	0.0	6.974248532520912	0.0
45	0.0	0.0	0.0	7.155257317697732	0.0
46	0.0	0.0	0.0	7.348977689065147	0.0
47	0.0	0.0	0.0	7.564286746070308	0.0
48	0.0	0.0	0.0	7.757954590222059	0.0
49	0.0	0.0	0.0	7.973788919383856	0.0
50	0.0	0.0	0.0	8.195348715052987	0.0
51	0.0	0.0	0.0	8.419482344289635	0.0
52	0.0	0.0	0.0	8.71605100392641	0.0
53	0.0	0.0	0.0	8.947853606649945	0.0
54	0.0	0.0	0.0	9.175769195414373	0.0
55	5.252721566361571E-5	0.0	0.0	9.40231907657155	0.0
56	5.252721566361571E-5	0.0	0.0	9.608593452482568	0.0
57	5.252721566361571E-5	0.0	0.0	9.84181429002902	0.0
58	5.252721566361571E-5	0.0	0.0	10.069099552205486	0.0
59	5.252721566361571E-5	0.0	0.0	10.30668014865202	0.0
60	5.252721566361571E-5	0.0	0.0	10.55581673254455	0.0
61	5.252721566361571E-5	0.0	0.0	10.823022678625362	0.0
62	5.252721566361571E-5	0.0	0.0	11.199695342149152	0.0
63	5.252721566361571E-5	0.0	0.0	11.51522632664049	0.0
64	5.252721566361571E-5	0.0	0.0	11.806857428004886	5.252721566361571E-5
65	5.252721566361571E-5	0.0	0.0	12.067865162637391	5.252721566361571E-5
66	5.252721566361571E-5	0.0	0.0	12.304605323633307	5.252721566361571E-5
67	5.252721566361571E-5	0.0	0.0	12.557208703759635	5.252721566361571E-5
68	5.252721566361571E-5	0.0	0.0	12.868222347703904	5.252721566361571E-5
69	5.252721566361571E-5	0.0	0.0	13.286076348307967	5.252721566361571E-5
70	5.252721566361571E-5	0.0	0.0	13.56998594896981	5.252721566361571E-5
71	5.252721566361571E-5	0.0	0.0	13.838347493795222	5.252721566361571E-5
72	1.0505443132723142E-4	0.0	0.0	14.134443408491025	5.252721566361571E-5
73	1.0505443132723142E-4	0.0	0.0	14.419718716760121	5.252721566361571E-5
74	1.0505443132723142E-4	0.0	0.0	14.74874919567701	5.252721566361571E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	50	0.0	80.0	1
CGGGATA	520	0.0	75.38462	6
CTAGCGG	305	0.0	73.44263	2
TCGAACG	60	0.0	73.333336	1
TATAGCG	175	0.0	73.14286	1
CGAGGGA	1095	0.0	72.69407	4
CGTTTTT	5100	0.0	72.4706	1
CGTTAGG	285	0.0	70.17544	2
CGAACGG	145	0.0	68.965515	2
ACGAGGG	825	0.0	68.84849	3
CGGGGTA	250	0.0	68.8	6
CGGGTCC	105	0.0	68.57143	6
ATAGCGG	350	0.0	68.57143	2
TACGGGA	520	0.0	68.46154	4
TTGCGAG	165	0.0	67.878784	1
TTAGGCG	60	0.0	66.66667	1
CGGGATC	630	0.0	66.666664	6
TAGCGGG	1325	0.0	65.81132	3
TAGGGTG	560	0.0	65.71429	5
CGTAAGG	470	0.0	65.53191	2
>>END_MODULE