FastQCFastQC Report
Thu 26 May 2016
SRR1547784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547784_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3532628
Sequences flagged as poor quality0
Sequence length86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431771.2222345517274957No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC35360.10009545301684752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT169150.074.182671
TACGGGA16350.070.948014
CGGGATA14150.069.257956
ACGGGAT22550.067.937925
TATAGCG3550.067.605631
TAGGGCG3850.066.493515
CGAGGGA28950.066.459414
TAGGGAT89750.066.3621145
AGGGATC68350.064.6086356
AGGGACC42250.064.568056
GTACGGG12700.064.251973
GCGGGAT33350.064.047975
TAGGGAC47450.063.983145
AGCGGGA30900.063.8187684
CGGGGTA7650.063.7908486
GGGACCG13900.063.3093537
CGTAAGG5000.063.22
TAGGGGT49150.063.0722275
GAGGGAT84300.062.9181485
GTAGGGA65550.062.852784
Produced by FastQC (version 0.11.5)