##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547784_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3532628
Sequences flagged as poor quality	0
Sequence length	86
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7517290243977	31.0	31.0	33.0	30.0	34.0
2	32.190704200951814	33.0	31.0	34.0	30.0	34.0
3	32.3144910814272	34.0	31.0	34.0	30.0	34.0
4	35.925972392224715	37.0	35.0	37.0	35.0	37.0
5	35.86875804641757	37.0	35.0	37.0	35.0	37.0
6	35.9313771503821	37.0	35.0	37.0	35.0	37.0
7	36.24711772651975	37.0	35.0	37.0	35.0	37.0
8	36.219489569804686	37.0	36.0	37.0	35.0	37.0
9	38.09110016678801	39.0	39.0	39.0	37.0	39.0
10-11	37.47905001602207	39.0	37.5	39.0	34.5	39.0
12-13	36.95477559482629	39.0	35.0	39.0	33.0	39.0
14-15	38.14073686785022	40.0	37.0	41.0	33.0	41.0
16-17	38.25437521301422	40.0	36.5	41.0	34.0	41.0
18-19	38.1065652822771	40.0	36.0	41.0	34.0	41.0
20-21	37.897014488930054	40.0	35.0	41.0	33.5	41.0
22-23	37.713789705567635	40.0	35.0	41.0	33.0	41.0
24-25	37.634913016598404	40.0	35.0	41.0	33.0	41.0
26-27	37.599045951059665	40.0	35.0	41.0	33.0	41.0
28-29	37.53729503927388	40.0	35.0	41.0	33.0	41.0
30-31	37.33134001089274	40.0	35.0	41.0	33.0	41.0
32-33	36.986495039953255	39.5	35.0	41.0	31.5	41.0
34-35	36.64862801857427	39.0	35.0	41.0	31.0	41.0
36-37	36.45819486229515	39.0	35.0	41.0	30.0	41.0
38-39	36.37013803887644	39.0	35.0	41.0	30.0	41.0
40-41	36.22630107104399	39.0	35.0	41.0	30.0	41.0
42-43	36.15600538749056	39.0	35.0	41.0	30.0	41.0
44-45	36.05027800832694	39.0	35.0	41.0	29.5	41.0
46-47	35.949386830427656	39.0	35.0	41.0	29.0	41.0
48-49	35.82811875464951	38.0	35.0	40.5	29.0	41.0
50-51	35.670086547465516	38.0	35.0	40.0	28.0	41.0
52-53	35.49861151527984	38.0	35.0	40.0	28.0	41.0
54-55	35.303628205404024	38.0	35.0	40.0	27.5	41.0
56-57	35.03305343783721	37.0	34.0	40.0	26.5	41.0
58-59	34.741787841799365	37.0	34.0	40.0	26.0	41.0
60-61	34.40294633343788	36.0	34.0	39.5	25.5	41.0
62-63	34.03987456363931	36.0	33.5	39.0	25.0	41.0
64-65	33.68678304084098	35.0	33.0	39.0	23.5	40.5
66-67	33.35223323825775	35.0	33.0	38.5	22.5	40.0
68-69	32.85796976075602	35.0	32.5	37.5	21.0	40.0
70-71	32.54086773359663	35.0	32.5	37.0	20.0	39.5
72-73	32.237759396120964	35.0	32.0	37.0	19.5	39.0
74-75	31.91372541914971	35.0	32.0	36.0	19.0	39.0
76-77	29.857268441511533	33.0	29.0	34.5	14.0	36.0
78-79	30.97394913928101	34.0	31.0	35.0	17.0	37.0
80-81	31.093215447536508	35.0	31.0	35.0	16.5	37.0
82-83	30.87408821421333	35.0	31.0	35.0	15.0	36.5
84-85	30.617523271626673	34.0	31.0	35.0	11.5	36.0
86	30.176489854012367	34.0	30.0	35.0	10.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	13.0
11	20.0
12	41.0
13	157.0
14	527.0
15	1717.0
16	4405.0
17	10030.0
18	20850.0
19	30818.0
20	33712.0
21	32362.0
22	28479.0
23	26267.0
24	27373.0
25	31136.0
26	36681.0
27	44792.0
28	54475.0
29	67498.0
30	83331.0
31	102783.0
32	123331.0
33	154243.0
34	314362.0
35	312633.0
36	314114.0
37	508705.0
38	749920.0
39	416209.0
40	1637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.457664945190942	23.042335620959804	31.97124067408173	14.528758759767516
2	29.659930227581278	25.610140665815926	31.559960460031455	13.169968646571334
3	26.857965231549997	24.93319421122179	35.289705001488976	12.919135555739238
4	25.47964291739747	26.670682562670056	34.507397891881055	13.342276628051412
5	23.604636548201512	31.53255876361734	31.73645795707898	13.12634673110217
6	22.41549350794932	39.98521780385594	27.365151383049675	10.234137305145065
7	88.21441148063141	2.9053724309494235	7.518510298848337	1.3617057895708238
8	88.91417381054558	2.324586681643241	7.234812156841876	1.5264273509693067
9	84.59110328061715	5.460778774328913	8.194805680077268	1.753312264976669
10-11	34.31083035066245	32.20709907751396	22.653234362633146	10.828836209190438
12-13	25.497377589715082	24.440359981294378	32.56892602334579	17.49333640564475
14-15	15.82319168618943	31.16715091427685	35.81046461727643	17.19919278225729
16-17	20.978475514546112	29.544492089175538	32.733661738513085	16.743370657765265
18-19	21.546876716144467	30.451239134151685	31.323111292782595	16.67877285692125
20-21	23.65662334103676	30.076829487848705	31.62515271916545	14.641394451949088
22-23	20.141619779948524	25.653904118973188	34.54380138525766	19.66067471582063
24-25	18.11729114981821	29.998644068948106	34.591542047450226	17.29252273378346
26-27	17.603693341048082	34.62354371872725	30.36239592733795	17.410367012886724
28-29	16.229135929398737	30.570088330840385	35.19469075147454	18.006084988286343
30-31	20.223456871201837	30.167852375059024	32.044712887968956	17.563977865770187
32-33	22.212146311471233	29.950790176605064	30.753648558523572	17.08341495340013
34-35	18.933411613110692	30.101159816431277	31.576350524312215	19.389078046145816
36-37	20.258685601767297	31.302220330020596	30.902942511920305	17.536151556291806
38-39	18.693972306169798	32.24648901610925	29.82978960705741	19.22974907066354
40-41	19.54844948293452	29.70343891289997	30.6112616443056	20.136849959859912
42-43	19.33409065432307	29.76735733284116	30.14244636004697	20.756105652788804
44-45	19.18783976122026	31.577482825816926	29.490693613932745	19.743983799030072
46-47	19.558045738186983	31.38029251877073	29.420278614108252	19.641383128934038
48-49	19.78365681300154	29.4882450119288	31.617905989535274	19.110192185534395
50-51	18.3605236667999	31.373781785118616	29.95766890824621	20.30802563983527
52-53	19.911507806652722	29.39509905939714	31.16205555750563	19.531337576444503
54-55	19.697531129799117	29.784172010186182	31.34838992387537	19.169906936139327
56-57	19.96967979645748	29.011447002061917	30.549692749986697	20.4691804514939
58-59	20.94955936486944	29.9981628408086	29.7476411328903	19.30463666143166
60-61	21.047291138495194	31.270742348189508	28.728017781662828	18.95394873165247
62-63	20.260553899250077	29.964575947424976	30.760923595691363	19.01394655763358
64-65	19.042947629923106	32.224126061391125	30.242938118590466	18.489988190095303
66-67	18.42213502242523	31.80984524835335	27.958236757450827	21.80978297177059
68-69	17.777232700414537	33.29110792305332	27.907509650039575	21.02414972649257
70-71	18.543744204031672	32.10282826269848	29.55611233336768	19.79731519990217
72-73	20.077941974077092	31.893380791863734	28.664637204936383	19.364040029122794
74-75	19.20190860741635	33.16530922587943	27.329257425350196	20.30352474135403
76-77	21.1102895634638	31.17428441375656	28.13599393992235	19.579432082857295
78-79	18.032142076663607	31.861662195962893	29.882186859188113	20.224008868185383
80-81	17.168691410474015	31.03502548244536	30.19692421619259	21.59935889088803
82-83	18.22739331738298	30.656865087408015	30.297755665187502	20.8179859300215
84-85	17.62155539728497	30.37015219264525	29.309709372172783	22.698583037897
86	18.331933053805834	30.811424242801678	29.758751841405324	21.097890861987167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2450.0
1	3150.5
2	15418.0
3	16268.5
4	6209.0
5	8590.0
6	12401.0
7	14488.0
8	15827.0
9	17204.5
10	16894.0
11	16128.0
12	14899.0
13	13819.0
14	13551.0
15	13332.5
16	13093.5
17	12927.0
18	13021.0
19	13864.0
20	15910.5
21	17354.0
22	17697.5
23	19967.0
24	24539.0
25	29183.0
26	34529.0
27	42518.5
28	51377.0
29	55594.0
30	59757.5
31	71398.0
32	81682.0
33	91384.5
34	101441.0
35	111016.5
36	117431.0
37	119967.0
38	130140.0
39	142213.0
40	154031.5
41	170379.5
42	179553.0
43	179761.0
44	177822.5
45	176908.5
46	175374.5
47	169478.5
48	159904.5
49	143153.5
50	132639.0
51	121683.5
52	100203.5
53	82721.0
54	72494.5
55	65978.0
56	56769.0
57	50808.0
58	43775.5
59	32053.0
60	23984.0
61	18669.0
62	14609.5
63	9726.0
64	6966.0
65	5680.5
66	3391.0
67	1892.0
68	1109.5
69	756.5
70	605.0
71	519.0
72	384.0
73	180.0
74	105.0
75	79.5
76	47.5
77	35.0
78	28.5
79	22.0
80	20.0
81	18.5
82	13.0
83	5.5
84	3.0
85	2.0
86	2.0
87	3.0
88	2.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3532628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.0314033702535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76513619789664	14.022145389269758
2	8.670702398608311	3.12689864905462
3	2.8852945048141043	1.5607772717483686
4	1.5718648049606325	1.1337171336700003
5	1.0516456934323146	0.9481323850434008
6	0.7476801855714349	0.808903381079072
7	0.6169919529912141	0.7787661546409548
8	0.49672292595766526	0.716528915295618
9	0.34941451965854403	0.5670390732647921
>10	3.2586675697656795	13.198493548818668
>50	1.1044703403549345	14.397414649784789
>100	1.473148074745002	46.05396049198783
>500	0.0050835884576466124	0.6166424894860644
>1k	0.003018380646727676	0.8337721438877167
>5k	0.0	0.0
>10k+	1.5886213930145664E-4	1.236808322968356
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43177	1.2222345517274957	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	3536	0.10009545301684752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.830753761788674E-5	0.0	0.0	0.05582246418247265	0.0
2	2.830753761788674E-5	0.0	0.0	0.1805737824644995	0.0
3	2.830753761788674E-5	0.0	0.0	0.26311856215825724	0.0
4	2.830753761788674E-5	0.0	0.0	0.39440892163001595	0.0
5	2.830753761788674E-5	0.0	0.0	0.7114816504879653	0.0
6	2.830753761788674E-5	0.0	0.0	0.9832906266949138	0.0
7	2.830753761788674E-5	0.0	0.0	1.2000131346974547	0.0
8	5.661507523577348E-5	0.0	0.0	1.601810323645739	0.0
9	5.661507523577348E-5	0.0	0.0	1.7578980860707665	0.0
10	5.661507523577348E-5	0.0	0.0	2.0068628794200807	0.0
11	5.661507523577348E-5	0.0	0.0	2.4623028521542603	0.0
12	5.661507523577348E-5	0.0	0.0	2.7790075830231773	0.0
13	5.661507523577348E-5	0.0	0.0	2.8846230058755125	0.0
14	5.661507523577348E-5	0.0	0.0	2.928895994709887	0.0
15	5.661507523577348E-5	0.0	0.0	2.994286406607206	0.0
16	5.661507523577348E-5	0.0	0.0	3.1238216987466556	0.0
17	5.661507523577348E-5	0.0	0.0	3.31410496661409	0.0
18	8.492261285366022E-5	0.0	0.0	3.5157112495286795	0.0
19	8.492261285366022E-5	0.0	0.0	3.6731860812969837	0.0
20	8.492261285366022E-5	0.0	0.0	3.834793813557499	0.0
21	8.492261285366022E-5	0.0	0.0	4.021114026158429	2.830753761788674E-5
22	8.492261285366022E-5	0.0	0.0	4.2431866587707505	2.830753761788674E-5
23	8.492261285366022E-5	0.0	0.0	4.463532531588381	2.830753761788674E-5
24	8.492261285366022E-5	0.0	0.0	4.624206115107507	2.830753761788674E-5
25	8.492261285366022E-5	0.0	0.0	4.765319190132672	2.830753761788674E-5
26	8.492261285366022E-5	0.0	0.0	4.895901861163983	2.830753761788674E-5
27	8.492261285366022E-5	0.0	0.0	5.036958321113913	2.830753761788674E-5
28	8.492261285366022E-5	0.0	0.0	5.19049840515333	2.830753761788674E-5
29	8.492261285366022E-5	0.0	0.0	5.364618069040952	2.830753761788674E-5
30	8.492261285366022E-5	0.0	0.0	5.579160896646916	2.830753761788674E-5
31	8.492261285366022E-5	0.0	0.0	5.7570171555000975	2.830753761788674E-5
32	8.492261285366022E-5	0.0	0.0	5.920578107856247	2.830753761788674E-5
33	8.492261285366022E-5	0.0	0.0	6.087252889350364	2.830753761788674E-5
34	8.492261285366022E-5	0.0	0.0	6.267373751212978	2.830753761788674E-5
35	8.492261285366022E-5	0.0	0.0	6.465073593936299	2.830753761788674E-5
36	8.492261285366022E-5	0.0	0.0	6.651846727139116	2.830753761788674E-5
37	8.492261285366022E-5	0.0	0.0	6.839525701545705	2.830753761788674E-5
38	8.492261285366022E-5	0.0	0.0	7.0546912949792615	2.830753761788674E-5
39	8.492261285366022E-5	0.0	0.0	7.326160580734796	2.830753761788674E-5
40	8.492261285366022E-5	0.0	0.0	7.548884286712329	2.830753761788674E-5
41	8.492261285366022E-5	0.0	0.0	7.772004298216512	2.830753761788674E-5
42	8.492261285366022E-5	0.0	0.0	7.997502142880598	2.830753761788674E-5
43	8.492261285366022E-5	0.0	0.0	8.21422465088314	2.830753761788674E-5
44	8.492261285366022E-5	0.0	0.0	8.431315156874712	2.830753761788674E-5
45	8.492261285366022E-5	0.0	0.0	8.647046901060627	2.830753761788674E-5
46	8.492261285366022E-5	0.0	0.0	8.873393971853249	2.830753761788674E-5
47	8.492261285366022E-5	0.0	0.0	9.112819125025336	2.830753761788674E-5
48	1.1323015047154696E-4	0.0	0.0	9.345252316405803	2.830753761788674E-5
49	1.415376880894337E-4	0.0	0.0	9.588215911780125	2.830753761788674E-5
50	1.415376880894337E-4	0.0	0.0	9.839275462913163	2.830753761788674E-5
51	1.415376880894337E-4	0.0	0.0	10.09596821403216	2.830753761788674E-5
52	1.415376880894337E-4	0.0	0.0	10.377741443480604	2.830753761788674E-5
53	1.415376880894337E-4	0.0	0.0	10.645077828743927	2.830753761788674E-5
54	1.415376880894337E-4	0.0	0.0	10.905592097441339	2.830753761788674E-5
55	1.6984522570732044E-4	0.0	0.0	11.173749401295579	2.830753761788674E-5
56	1.6984522570732044E-4	0.0	0.0	11.435622431798649	2.830753761788674E-5
57	1.6984522570732044E-4	0.0	0.0	11.7132910682925	2.830753761788674E-5
58	1.6984522570732044E-4	0.0	0.0	11.980882221394385	2.830753761788674E-5
59	1.9815276332520718E-4	0.0	0.0	12.259654851855332	2.830753761788674E-5
60	1.9815276332520718E-4	0.0	0.0	12.566791635009404	2.830753761788674E-5
61	1.9815276332520718E-4	0.0	0.0	12.865096466426694	2.830753761788674E-5
62	1.9815276332520718E-4	0.0	0.0	13.197030652534034	2.830753761788674E-5
63	1.9815276332520718E-4	0.0	0.0	13.517188902992332	2.830753761788674E-5
64	1.9815276332520718E-4	0.0	0.0	13.835252395666908	2.830753761788674E-5
65	2.2646030094309393E-4	0.0	0.0	14.134463068287971	2.830753761788674E-5
66	2.2646030094309393E-4	0.0	0.0	14.428748229363523	2.830753761788674E-5
67	2.5476783856098067E-4	0.0	0.0	14.72883643565074	2.830753761788674E-5
68	3.1138291379675416E-4	0.0	0.0	15.051429134344177	2.830753761788674E-5
69	3.1138291379675416E-4	0.0	0.0	15.458265064988446	2.830753761788674E-5
70	3.1138291379675416E-4	0.0	0.0	15.778819620973394	2.830753761788674E-5
71	3.1138291379675416E-4	0.0	0.0	16.095496044304692	2.830753761788674E-5
72	3.396904514146409E-4	0.0	0.0	16.41571090983823	2.830753761788674E-5
73	3.6799798903252765E-4	0.0	0.0	16.732160872868583	2.830753761788674E-5
74	3.9630552665041437E-4	0.0	0.0	17.085297404651723	2.830753761788674E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16915	0.0	74.18267	1
TACGGGA	1635	0.0	70.94801	4
CGGGATA	1415	0.0	69.25795	6
ACGGGAT	2255	0.0	67.93792	5
TATAGCG	355	0.0	67.60563	1
TAGGGCG	385	0.0	66.49351	5
CGAGGGA	2895	0.0	66.45941	4
TAGGGAT	8975	0.0	66.362114	5
AGGGATC	6835	0.0	64.608635	6
AGGGACC	4225	0.0	64.56805	6
GTACGGG	1270	0.0	64.25197	3
GCGGGAT	3335	0.0	64.04797	5
TAGGGAC	4745	0.0	63.98314	5
AGCGGGA	3090	0.0	63.818768	4
CGGGGTA	765	0.0	63.790848	6
GGGACCG	1390	0.0	63.309353	7
CGTAAGG	500	0.0	63.2	2
TAGGGGT	4915	0.0	63.072227	5
GAGGGAT	8430	0.0	62.918148	5
GTAGGGA	6555	0.0	62.85278	4
>>END_MODULE