FastQCFastQC Report
Thu 26 May 2016
SRR1547783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547783_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2781317
Sequences flagged as poor quality0
Sequence length86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355661.2787467232250045No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC42880.15417156692315187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37800.13590683837908443No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33210.11940386514733847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105750.075.0070951
GCGCGAC700.074.285719
TACGCGG1250.070.42
TGCGTAG2150.068.837211
CGGGATA9650.067.5647666
GAACGAC1200.066.666679
TCGCGCG303.7970003E-666.666671
CGGGACG900.066.6666646
CGGGAGT8650.065.6647346
TATTGCG3850.065.4545441
CGAACGG2150.065.116282
ATTGCGG8350.064.670662
ATAACGG2300.064.3478242
GTTAGCG2550.064.313731
AGGGATC30650.063.556286
TAGGGCG6050.063.4710735
ATAGGGA32350.063.4312254
AGGGATT46500.063.1397866
TTGTGCG3300.063.0303041
GATTGCG2550.062.74511
Produced by FastQC (version 0.11.5)