##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547783_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2781317
Sequences flagged as poor quality	0
Sequence length	86
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.912214968664127	31.0	31.0	33.0	31.0	34.0
2	32.371019916104494	33.0	31.0	34.0	31.0	34.0
3	32.508444380845475	34.0	31.0	34.0	31.0	34.0
4	36.09002965142053	37.0	35.0	37.0	35.0	37.0
5	36.057082310286816	37.0	35.0	37.0	35.0	37.0
6	36.110863306843484	37.0	35.0	37.0	35.0	37.0
7	36.31452617590875	37.0	36.0	37.0	35.0	37.0
8	36.270574695369135	37.0	37.0	37.0	35.0	37.0
9	38.15339172054102	39.0	39.0	39.0	37.0	39.0
10-11	37.68665671694381	39.0	37.5	39.0	35.0	39.0
12-13	36.90963777232153	39.0	35.0	39.0	33.5	39.0
14-15	38.018770603998036	40.0	35.0	41.0	33.0	41.0
16-17	38.19426660103829	40.0	35.0	41.0	34.0	41.0
18-19	38.09316989037927	40.0	36.0	41.0	34.0	41.0
20-21	37.893977025991646	40.0	35.0	41.0	34.0	41.0
22-23	37.67957014608547	39.5	35.0	41.0	34.0	41.0
24-25	37.56718543769013	39.0	35.0	41.0	34.0	41.0
26-27	37.46549997716909	39.0	35.0	41.0	34.0	41.0
28-29	37.40632279599916	39.0	35.0	41.0	34.0	41.0
30-31	37.215552560172036	39.0	35.0	41.0	33.0	41.0
32-33	36.908404543602906	39.0	35.0	41.0	33.0	41.0
34-35	36.64580808300528	38.0	35.0	41.0	32.5	41.0
36-37	36.4270690827403	38.0	35.0	41.0	32.0	41.0
38-39	36.325586583622076	38.0	35.0	41.0	31.0	41.0
40-41	36.188842551927735	38.0	35.0	41.0	31.0	41.0
42-43	36.1363760405592	38.0	35.0	41.0	31.0	41.0
44-45	36.02259361302578	38.0	35.0	40.5	31.0	41.0
46-47	35.951174749228514	38.0	35.0	40.0	31.0	41.0
48-49	35.84157415353949	37.0	35.0	40.0	31.0	41.0
50-51	35.66544086848065	37.0	35.0	40.0	30.0	41.0
52-53	35.50526351365198	36.5	35.0	40.0	30.0	41.0
54-55	35.3305417900944	36.0	35.0	40.0	29.5	41.0
56-57	35.04428855107131	36.0	34.5	40.0	28.5	41.0
58-59	34.74834386012094	35.5	34.0	39.5	28.0	41.0
60-61	34.426914659494045	35.0	34.0	39.0	27.0	41.0
62-63	33.96634921513801	35.0	33.5	39.0	26.0	40.5
64-65	33.62470369253127	35.0	33.0	38.0	26.0	40.0
66-67	33.29332830454062	35.0	33.0	37.0	24.5	40.0
68-69	32.862295272347595	35.0	33.0	37.0	22.5	39.5
70-71	32.5546027295702	35.0	33.0	36.0	21.5	39.0
72-73	32.243162501793215	35.0	33.0	36.0	20.0	39.0
74-75	31.94856645251153	35.0	33.0	35.5	19.5	38.0
76-77	29.959539851084934	33.0	29.5	34.5	16.0	36.0
78-79	30.969528464392948	34.0	31.0	35.0	17.5	37.0
80-81	31.213087001589535	35.0	32.0	35.0	17.5	36.0
82-83	31.04694790273816	35.0	32.0	35.0	16.0	36.0
84-85	30.82990252459536	35.0	32.0	35.0	13.5	36.0
86	30.448344075846084	34.0	31.0	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	17.0
10	8.0
11	22.0
12	56.0
13	103.0
14	310.0
15	910.0
16	2260.0
17	5538.0
18	11332.0
19	17494.0
20	20302.0
21	20854.0
22	18722.0
23	17586.0
24	18668.0
25	21724.0
26	26176.0
27	32233.0
28	40054.0
29	50381.0
30	64997.0
31	80348.0
32	99064.0
33	129900.0
34	332482.0
35	356444.0
36	255949.0
37	370938.0
38	519089.0
39	266016.0
40	1331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.710127612206733	24.471356555185906	30.609671605214366	14.208844227392994
2	30.088911116568156	24.78358274155733	31.606501524277885	13.521004617596628
3	28.602888487720023	24.716959627399536	33.406871636710235	13.273280248170202
4	25.39527137683335	28.03553136877242	32.817079103173064	13.752118151221165
5	24.35889184871771	31.760816907961225	30.65882098300913	13.221470260311932
6	22.212318840319174	40.386874275747786	26.824486385406626	10.576320498526417
7	86.37127662902144	4.531666113571377	7.2955725650833765	1.8014846923238164
8	87.38252417829395	3.6760642530139496	6.902413496915311	2.038998071776788
9	82.60263033663549	6.274401659357778	8.410116502362012	2.7128515016447246
10-11	41.57604113446975	27.365201449529124	19.581011441701897	11.477745974299227
12-13	28.40618311397083	29.986909079403752	25.648550668622093	15.958357138003326
14-15	15.108489970758457	35.807712677123824	33.76752811707547	15.316269235042249
16-17	18.065020995449277	30.318550528400756	32.10322663687742	19.513201839272547
18-19	22.0426510174856	32.48049395304455	28.9089305534033	16.567924476066555
20-21	24.397111152738074	33.03496149486017	27.547848734969797	15.02007861743196
22-23	19.240057857482622	30.89511192000049	28.942421881432427	20.92240834108446
24-25	17.357208833081593	32.190469479027385	33.32266692361927	17.129654764271745
26-27	15.893136237257385	40.262580640754	27.318658750512796	16.525624371475814
28-29	15.278930808678048	33.786889448415984	34.03315767314549	16.901022069760476
30-31	20.74438116906487	35.16819549875113	28.186107516690832	15.901315815493163
32-33	24.53673924978706	33.766359605898934	25.938359417498972	15.75854172681503
34-35	18.217862257340677	34.57007957021799	28.13178073552925	19.080277436912084
36-37	21.73953921829119	35.26268311019564	27.92804631762579	15.069731353887386
38-39	18.77218598239611	37.024941781177766	25.89427958050089	18.30859265592523
40-41	19.348945122041105	32.80978040259345	27.176693631110727	20.66458084425472
42-43	20.09404177948792	31.10023057422077	26.981785966863896	21.82394167942741
44-45	17.76162156273449	37.313707858543275	26.04544897255509	18.87922160616715
46-47	19.832241344657945	35.64437279173859	26.48148341235465	18.041902451248816
48-49	19.96942455678371	31.70037791449159	29.982900187213467	18.347297341511233
50-51	17.474041973640546	36.76736596367836	27.309005050485073	18.44958701219602
52-53	20.597292577581054	31.78647022256003	28.872059531509713	18.744177668349202
54-55	20.836423895586158	32.69580562014326	28.935572608228405	17.532197876042176
56-57	21.53278464842375	30.390135320785085	27.790503563599543	20.286576467191622
58-59	22.477355152253413	32.72796664314065	26.866426948096887	17.928251256509057
60-61	22.34288648147622	35.13664569698456	25.245432289810903	17.275035531728314
62-63	21.23371769560967	31.849264215477774	29.419390166600927	17.497627922311622
64-65	17.982524106385572	36.08630371870592	28.97790866700919	16.953263507899315
66-67	16.876609174718308	35.09118162366965	24.36536360292624	23.6668455986858
68-69	16.3401367050214	37.670732965713725	24.022126927638958	21.96700340162592
70-71	17.915954923512853	35.50760305279837	27.545817323232125	19.030624700456656
72-73	20.84580793918852	35.83773442581338	25.48603772960795	17.830419905390144
74-75	20.077592737541245	37.515950177559766	23.058105206993666	19.348351877905323
76-77	24.784445642118463	33.0976656023028	24.35650089507956	17.76138786049918
78-79	18.03940363504052	34.437876013413785	28.940444400979825	18.582275950565865
80-81	15.616648515793058	33.51525554260805	29.887783377443135	20.98031256415576
82-83	17.49112021391305	31.589135650485005	30.37009085983367	20.54965327576828
84-85	16.104564851830986	31.51533967541276	28.611679287186607	23.76841618556964
86	16.861832002608836	31.49856704575566	30.25750031370031	21.382100637935196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3172.0
1	4323.0
2	16214.0
3	16382.0
4	7956.5
5	15611.5
6	27211.5
7	33303.0
8	35071.5
9	34246.0
10	27329.0
11	19949.0
12	14945.0
13	12295.5
14	11593.0
15	10970.0
16	10014.5
17	9608.0
18	9429.5
19	9111.0
20	9204.5
21	9512.0
22	9810.5
23	11390.5
24	12950.5
25	15793.5
26	20757.5
27	24638.0
28	34693.0
29	43267.0
30	42683.0
31	46555.5
32	51005.5
33	53702.0
34	57835.0
35	67020.5
36	74776.0
37	78294.5
38	85859.0
39	94641.0
40	103938.5
41	119256.0
42	133734.5
43	137457.0
44	143509.0
45	149514.5
46	151859.0
47	155100.5
48	149399.5
49	129183.0
50	115518.0
51	97359.5
52	73351.5
53	64929.0
54	58749.5
55	54699.5
56	50005.5
57	45755.0
58	41854.0
59	31514.5
60	20606.5
61	15309.0
62	12959.0
63	9450.0
64	7463.0
65	6181.5
66	3763.0
67	2269.5
68	1410.0
69	759.5
70	591.5
71	571.0
72	403.5
73	154.0
74	80.5
75	79.0
76	67.0
77	63.5
78	49.5
79	37.0
80	26.5
81	15.0
82	10.0
83	4.5
84	3.0
85	3.0
86	3.0
87	2.5
88	3.0
89	2.5
90	1.0
91	1.5
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2781317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.085808991320413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7538091607278	11.428074953636779
2	9.39011362499467	2.833149211069298
3	3.304974623609311	1.4957464767879343
4	1.7438651271433598	1.0523046485883762
5	1.2170436395608453	0.918004394025816
6	0.9374238490455122	0.8485078278365387
7	0.7600097759289894	0.8025753617840673
8	0.629330668078605	0.7595169800811122
9	0.5338699009850151	0.7248473417227572
>10	3.453108776370876	9.92212266422322
>50	0.6521529467123639	7.113446174177036
>100	1.5788848442052612	56.053422091082524
>500	0.04156857056227078	3.7163565162551664
>1k	0.0036042113204281015	1.0427184739975237
>5k	0.0	0.0
>10k+	2.4028075469520682E-4	1.2892068847318334
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35566	1.2787467232250045	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	4288	0.15417156692315187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3780	0.13590683837908443	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3321	0.11940386514733847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.595419004737684E-5	0.0	0.0	0.07043425830281122	0.0
2	3.595419004737684E-5	0.0	0.0	0.22478559617619998	0.0
3	3.595419004737684E-5	0.0	0.0	0.33282793726856735	0.0
4	3.595419004737684E-5	0.0	0.0	0.49440606734147885	0.0
5	3.595419004737684E-5	0.0	0.0	0.8936773478175987	0.0
6	3.595419004737684E-5	0.0	0.0	1.2226581867510966	0.0
7	3.595419004737684E-5	0.0	0.0	1.478292477987946	0.0
8	3.595419004737684E-5	0.0	0.0	1.9423172547393914	0.0
9	3.595419004737684E-5	0.0	0.0	2.109468284269646	0.0
10	3.595419004737684E-5	0.0	0.0	2.366972193388959	0.0
11	3.595419004737684E-5	0.0	0.0	2.8830586373290066	0.0
12	3.595419004737684E-5	0.0	0.0	3.21265069749331	0.0
13	3.595419004737684E-5	0.0	0.0	3.3142212843771492	0.0
14	3.595419004737684E-5	0.0	0.0	3.353123718008411	0.0
15	3.595419004737684E-5	0.0	0.0	3.4142458410889516	0.0
16	3.595419004737684E-5	0.0	0.0	3.547923519685099	0.0
17	3.595419004737684E-5	0.0	0.0	3.7247821805281456	0.0
18	3.595419004737684E-5	0.0	0.0	3.912786640285879	0.0
19	3.595419004737684E-5	0.0	0.0	4.040747602664493	0.0
20	3.595419004737684E-5	0.0	0.0	4.152996583992404	0.0
21	3.595419004737684E-5	0.0	0.0	4.293829146407979	0.0
22	3.595419004737684E-5	0.0	0.0	4.454400559159564	0.0
23	3.595419004737684E-5	0.0	0.0	4.616482047893139	0.0
24	3.595419004737684E-5	0.0	0.0	4.739589194615356	0.0
25	3.595419004737684E-5	0.0	0.0	4.841735048539954	0.0
26	3.595419004737684E-5	0.0	0.0	4.937121514735645	0.0
27	3.595419004737684E-5	0.0	0.0	5.033011339591999	0.0
28	3.595419004737684E-5	0.0	0.0	5.1348695599962175	0.0
29	3.595419004737684E-5	0.0	0.0	5.257401439677678	0.0
30	3.595419004737684E-5	0.0	0.0	5.395825071360079	0.0
31	3.595419004737684E-5	0.0	0.0	5.525619697431109	0.0
32	3.595419004737684E-5	0.0	0.0	5.641320281003567	0.0
33	3.595419004737684E-5	0.0	0.0	5.757775902567022	0.0
34	3.595419004737684E-5	0.0	0.0	5.885952590085919	0.0
35	3.595419004737684E-5	0.0	0.0	6.038110722366419	0.0
36	3.595419004737684E-5	0.0	0.0	6.1742692400758346	0.0
37	3.595419004737684E-5	0.0	0.0	6.315856840482405	0.0
38	3.595419004737684E-5	0.0	0.0	6.468770010753898	0.0
39	3.595419004737684E-5	0.0	0.0	6.667956223616366	0.0
40	3.595419004737684E-5	0.0	0.0	6.838163359300648	0.0
41	3.595419004737684E-5	0.0	0.0	7.006069426821898	0.0
42	3.595419004737684E-5	0.0	0.0	7.171099159139358	0.0
43	3.595419004737684E-5	0.0	0.0	7.316102407600428	0.0
44	3.595419004737684E-5	0.0	0.0	7.47670977454206	0.0
45	3.595419004737684E-5	0.0	0.0	7.634656531420187	0.0
46	3.595419004737684E-5	0.0	0.0	7.8100770246613385	0.0
47	3.595419004737684E-5	0.0	0.0	7.995277057595376	0.0
48	3.595419004737684E-5	0.0	0.0	8.176809763144583	0.0
49	3.595419004737684E-5	0.0	0.0	8.370063534649233	0.0
50	3.595419004737684E-5	0.0	0.0	8.557241048035877	0.0
51	3.595419004737684E-5	0.0	0.0	8.7522206206628	0.0
52	3.595419004737684E-5	0.0	0.0	8.978444384440897	0.0
53	3.595419004737684E-5	0.0	0.0	9.170080217393414	0.0
54	3.595419004737684E-5	0.0	0.0	9.37476742133313	0.0
55	3.595419004737684E-5	0.0	0.0	9.589917294576635	0.0
56	7.190838009475368E-5	0.0	0.0	9.795790986787914	0.0
57	7.190838009475368E-5	0.0	0.0	10.010437501370754	0.0
58	1.078625701421305E-4	0.0	0.0	10.213938217038907	0.0
59	1.078625701421305E-4	0.0	0.0	10.428764502571983	0.0
60	1.078625701421305E-4	0.0	0.0	10.659949944576615	0.0
61	1.078625701421305E-4	0.0	0.0	10.89577347709736	0.0
62	1.4381676018950735E-4	0.0	0.0	11.146697769438004	0.0
63	1.4381676018950735E-4	0.0	0.0	11.423545032802805	0.0
64	1.4381676018950735E-4	0.0	0.0	11.674828867043923	0.0
65	1.4381676018950735E-4	0.0	0.0	11.916800566062768	0.0
66	1.4381676018950735E-4	0.0	0.0	12.136013262781624	0.0
67	1.4381676018950735E-4	0.0	0.0	12.378380457890993	0.0
68	1.4381676018950735E-4	0.0	0.0	12.636423679861016	0.0
69	1.4381676018950735E-4	0.0	0.0	12.971516731102568	0.0
70	1.7977095023688418E-4	0.0	0.0	13.216904078175915	0.0
71	1.7977095023688418E-4	0.0	0.0	13.462830738099973	0.0
72	1.7977095023688418E-4	0.0	0.0	13.712892129879478	0.0
73	1.7977095023688418E-4	0.0	0.0	13.97877336527983	0.0
74	1.7977095023688418E-4	0.0	0.0	14.260330627540837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10575	0.0	75.007095	1
GCGCGAC	70	0.0	74.28571	9
TACGCGG	125	0.0	70.4	2
TGCGTAG	215	0.0	68.83721	1
CGGGATA	965	0.0	67.564766	6
GAACGAC	120	0.0	66.66667	9
TCGCGCG	30	3.7970003E-6	66.66667	1
CGGGACG	90	0.0	66.666664	6
CGGGAGT	865	0.0	65.664734	6
TATTGCG	385	0.0	65.454544	1
CGAACGG	215	0.0	65.11628	2
ATTGCGG	835	0.0	64.67066	2
ATAACGG	230	0.0	64.347824	2
GTTAGCG	255	0.0	64.31373	1
AGGGATC	3065	0.0	63.55628	6
TAGGGCG	605	0.0	63.471073	5
ATAGGGA	3235	0.0	63.431225	4
AGGGATT	4650	0.0	63.139786	6
TTGTGCG	330	0.0	63.030304	1
GATTGCG	255	0.0	62.7451	1
>>END_MODULE