FastQCFastQC Report
Thu 26 May 2016
SRR1547782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547782_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3161636
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141450.44739495628212733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32010.1012450516125196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92050.072.178171
TAGGGCG6750.068.740745
CGTAAGG4700.067.234052
AGGGCGA17100.066.900586
GGGCGAT25850.066.847197
CGTTAGG4750.066.526312
CGGGTCC3550.066.4788746
TAGGTCG1450.066.20691
TACGGGT4850.065.9793854
GGCGATC4650.065.376348
GCGAACG750.064.01
TTACGTA251.2358601E-464.080
TAATGCG1200.063.3333321
GCGGGTC5200.063.076925
GTTACGG3950.062.7848132
ACGGGAT15250.062.6885265
TGTTACG2050.062.4390261
TCGATAG3150.062.222221
TTACGGG11650.061.802573
CGAAGGG16200.061.481483
Produced by FastQC (version 0.11.5)