##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547782_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3161636
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.756885043059984	31.0	31.0	33.0	30.0	34.0
2	32.20175820366418	33.0	31.0	34.0	31.0	34.0
3	32.36354026839269	34.0	31.0	34.0	30.0	34.0
4	35.95879253652223	37.0	35.0	37.0	35.0	37.0
5	35.91217426674039	37.0	35.0	37.0	35.0	37.0
6	35.95954436247563	37.0	35.0	37.0	35.0	37.0
7	36.22597952452465	37.0	35.0	37.0	35.0	37.0
8	36.18578198122744	37.0	35.0	37.0	35.0	37.0
9	38.05831632736975	39.0	39.0	39.0	35.0	39.0
10-11	37.481139669462266	39.0	37.0	39.0	35.0	39.0
12-13	36.719487948644314	39.0	35.0	39.0	33.0	39.0
14-15	37.80283609498373	40.0	35.0	41.0	33.0	41.0
16-17	37.937501344240765	40.0	35.0	41.0	33.5	41.0
18-19	37.83420339975886	40.0	36.0	41.0	33.5	41.0
20-21	37.66100857277688	40.0	35.0	41.0	33.5	41.0
22-23	37.45153679930264	39.0	35.0	41.0	33.0	41.0
24-25	37.37499683708055	39.0	35.0	41.0	33.0	41.0
26-27	37.34290079566402	39.0	35.0	41.0	33.0	41.0
28-29	37.36158257939877	39.0	35.0	41.0	33.0	41.0
30-31	37.211970005402264	39.0	35.0	41.0	33.0	41.0
32-33	36.90549038535745	39.0	35.0	41.0	32.0	41.0
34-35	36.64230227641639	39.0	35.0	41.0	31.0	41.0
36-37	36.457106700455086	39.0	35.0	41.0	31.0	41.0
38-39	36.363407267629796	39.0	35.0	41.0	31.0	41.0
40-41	36.20051422744427	38.0	35.0	41.0	30.0	41.0
42-43	36.15220268873456	38.0	35.0	41.0	30.0	41.0
44-45	36.055675605920484	38.0	35.0	41.0	30.0	41.0
46-47	35.94302728081284	38.0	35.0	40.5	30.0	41.0
48-49	35.852294824578166	38.0	35.0	40.0	30.0	41.0
50-51	35.66828676672457	38.0	35.0	40.0	29.0	41.0
52-53	35.49459457065899	37.0	35.0	40.0	29.0	41.0
54-55	35.30919593526896	37.0	34.5	40.0	28.5	41.0
56-57	35.00330019015472	36.0	34.0	40.0	27.5	41.0
58-59	34.71899943573517	36.0	34.0	40.0	27.0	41.0
60-61	34.39281878116266	35.5	34.0	39.0	26.0	41.0
62-63	33.97635417233356	35.0	33.5	39.0	26.0	40.5
64-65	33.701977235836125	35.0	33.0	38.5	26.0	40.0
66-67	33.43934927992976	35.0	33.0	38.0	25.0	40.0
68-69	33.07028687046832	35.0	33.0	37.0	24.0	40.0
70-71	32.814128824444055	35.0	33.0	36.5	24.0	39.0
72-73	32.5457699747852	35.0	33.0	36.0	23.5	39.0
74-75	32.24855612727082	35.0	32.5	36.0	23.0	38.5
76-77	30.193773729803176	33.0	29.5	34.5	19.5	36.0
78-79	31.29704194284225	34.0	31.0	35.0	20.0	37.0
80-81	31.53941851623653	35.0	32.0	35.0	20.0	36.5
82-83	31.385454872097863	35.0	32.0	35.0	20.0	36.0
84-85	31.211652131997482	34.0	32.0	35.0	19.0	36.0
86	30.785260226034875	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	13.0
11	22.0
12	51.0
13	129.0
14	446.0
15	1357.0
16	3448.0
17	7451.0
18	14700.0
19	21939.0
20	25439.0
21	24853.0
22	22059.0
23	20900.0
24	22507.0
25	26235.0
26	31307.0
27	37706.0
28	46284.0
29	57717.0
30	71705.0
31	89024.0
32	110074.0
33	146874.0
34	355825.0
35	337290.0
36	273483.0
37	468785.0
38	640760.0
39	301786.0
40	1448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.642225733765684	23.058631670438974	32.086837320931316	13.212305274864026
2	29.558810691679877	25.43325670633811	31.196348978819827	13.811583623162186
3	28.871539924267058	25.283650616326486	32.534358793991466	13.310450665414994
4	26.278230637556	28.209319478902696	31.329919067217098	14.182530816324206
5	24.47207711450654	32.13949992978319	29.43191436332329	13.95650859238698
6	21.958599914727692	41.46154079723283	26.147001109552143	10.43285817848734
7	88.47416337617614	3.9145556287947127	6.069610796435769	1.5416701985933865
8	89.45447863068361	3.1471997408936385	5.736650265875009	1.6616713625477442
9	84.6252066967861	5.849155310731533	7.247481999825407	2.2781559926569663
10-11	40.56316097109218	26.629694246902552	20.209284054204847	12.597860727800416
12-13	29.006153776083014	27.826036899883476	25.986609464214094	17.18119985981941
14-15	17.04247421271772	32.773522948245784	33.63782231730661	16.546180521729887
16-17	21.353644126015773	25.288869433419915	32.76408163368585	20.593404806878464
18-19	24.179301475565182	28.307464869453664	29.3218447664437	18.191388888537453
20-21	26.756464058481118	28.397592259197452	28.141775334035923	16.70416834828551
22-23	22.00427247159382	25.215110151832786	30.225807145414592	22.554810231158807
24-25	19.69102388763286	26.981031339471084	34.57183243105785	18.756112341838215
26-27	18.262981570300944	34.597404634815646	28.861798132359322	18.277815662524084
28-29	16.80003643683207	29.293615710347424	35.74595557489857	18.160392277921936
30-31	22.362710318328865	29.750151503841682	29.63968654203077	18.24745163579868
32-33	25.320024190007956	28.77227169731114	27.999744436108394	17.907959676572506
34-35	19.667760615073966	29.501308816068644	29.836847125981613	20.994083442875777
36-37	22.57989218240177	30.00555724947464	29.188274678046426	18.22627589007716
38-39	20.21475590485432	31.412550337863053	27.944820339849368	20.427873417433254
40-41	20.965759499195986	27.29147188354384	28.21278287570106	23.529985741559116
42-43	21.69025782854193	26.558749963626425	28.28385367575521	23.467138532076433
44-45	19.658856996820635	31.716380380284132	27.713610928013217	20.911151694882015
46-47	21.363670580674057	30.770019066078447	27.275736359277285	20.59057399397021
48-49	21.603309172845957	27.188582113817024	30.92441697905768	20.28369173427934
50-51	19.264346053751918	31.659511088563008	28.250769538302322	20.825373319382752
52-53	22.07021934213806	26.506925528428955	29.84688939523715	21.57596573419584
54-55	21.91751359106488	27.601153326948452	30.49645816279926	19.98487491918741
56-57	22.80020533673073	25.81391406221336	29.24391043118183	22.141970169874078
58-59	23.29874470052846	27.942653740025737	28.148022099950786	20.61057945949502
60-61	24.038108751292054	29.368671788909285	26.742215106356333	19.85100435344233
62-63	22.947961118863777	26.315394941100113	31.07018012193687	19.666463818099235
64-65	19.988955085278633	30.465556439767262	29.853041273568493	19.692447201385612
66-67	18.795095324066402	29.304780816007913	26.644670670500968	25.255453189424713
68-69	18.54803968578293	32.224266171058275	25.453878941155782	23.773815202003014
70-71	19.203301708356054	30.568398765702316	28.48544234693684	21.742857179004794
72-73	22.60208006234747	30.033691417987395	26.999502789062372	20.364725730602764
74-75	21.495184771428463	31.877341351123277	24.868991876357683	21.758482001090574
76-77	25.11541493075104	28.55595963608714	26.14356934194828	20.185056091213536
78-79	19.15203078406243	29.91027430102643	29.5516308645271	21.38606405038404
80-81	17.2030556332228	28.936379773003594	30.07208926011723	23.788475333656372
82-83	18.92313030342519	27.953882104075234	31.409150199453702	21.71383739304588
84-85	17.422593872286374	27.642366167389287	29.448614578022266	25.486425382302073
86	18.795079509469147	27.387719522424465	31.071635064884127	22.745565903222257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2604.0
1	2707.0
2	5890.5
3	5886.0
4	3256.5
5	4248.5
6	5243.5
7	5702.0
8	5917.5
9	6120.5
10	5855.0
11	5487.5
12	5588.0
13	5854.0
14	5905.0
15	5977.0
16	6536.5
17	7110.5
18	7656.5
19	8316.5
20	8852.0
21	9187.0
22	9597.0
23	11021.0
24	12578.5
25	15598.5
26	19771.5
27	25491.0
28	33361.5
29	37209.0
30	41175.0
31	48568.5
32	53248.0
33	58167.5
34	67122.0
35	77760.5
36	83112.0
37	86039.0
38	92201.0
39	100704.0
40	117692.0
41	142882.0
42	166667.5
43	176983.0
44	175296.5
45	178038.0
46	190355.0
47	201583.5
48	195833.5
49	165730.5
50	144717.0
51	134051.5
52	112701.0
53	94053.5
54	82681.0
55	74290.0
56	64713.0
57	60117.0
58	55178.0
59	42561.0
60	29259.0
61	21467.0
62	17734.5
63	13672.0
64	11174.0
65	8672.0
66	5495.0
67	3678.0
68	2297.5
69	1638.0
70	1080.0
71	943.0
72	731.5
73	477.5
74	368.0
75	199.0
76	90.0
77	70.0
78	77.0
79	97.0
80	56.0
81	14.0
82	12.5
83	9.5
84	6.0
85	4.0
86	3.0
87	4.5
88	3.0
89	0.5
90	0.5
91	0.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	3161636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.105305973515406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04915394430509	11.940616572762508
2	9.568185472395307	2.890607383437523
3	3.0066385869236036	1.3624858742177712
4	1.39421888576015	0.8424041145384316
5	0.782959977468846	0.5913425012341824
6	0.5183096821812987	0.4697535803030426
7	0.38823746401353937	0.4105111979014332
8	0.30607454291607067	0.3698679697160896
9	0.22537777129641476	0.3063960175555191
>10	2.1549860020941245	7.242555276621972
>50	0.7623990470936507	8.534411079227556
>100	1.8079912569547065	60.888105639098065
>500	0.031878406882048876	2.9122866160377985
>1k	0.003377844437832994	0.7875776636621864
>5k	0.0	0.0
>10k+	2.1111527736456212E-4	0.45107851368593865
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14145	0.44739495628212733	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3201	0.1012450516125196	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.488758351688809E-5	0.0	0.0	0.0507332279870295	0.0
2	9.488758351688809E-5	0.0	0.0	0.1634913063995982	0.0
3	9.488758351688809E-5	0.0	0.0	0.2341825561196798	0.0
4	9.488758351688809E-5	0.0	0.0	0.34611827547510216	0.0
5	9.488758351688809E-5	0.0	0.0	0.5642332007859222	0.0
6	9.488758351688809E-5	0.0	0.0	0.7495802805889104	0.0
7	9.488758351688809E-5	0.0	0.0	0.8704354327949201	0.0
8	9.488758351688809E-5	0.0	0.0	1.1030048999948128	0.0
9	9.488758351688809E-5	0.0	0.0	1.1800852470050316	0.0
10	9.488758351688809E-5	0.0	0.0	1.3402871171760442	0.0
11	9.488758351688809E-5	0.0	0.0	1.6252977888662705	0.0
12	9.488758351688809E-5	0.0	0.0	1.8292744642330743	0.0
13	9.488758351688809E-5	0.0	0.0	1.904393801183944	0.0
14	9.488758351688809E-5	0.0	0.0	1.935896478911551	0.0
15	9.488758351688809E-5	0.0	0.0	1.988274425012873	0.0
16	9.488758351688809E-5	0.0	0.0	2.1114701376122995	0.0
17	9.488758351688809E-5	0.0	0.0	2.2573756118667676	0.0
18	9.488758351688809E-5	0.0	0.0	2.435542864516978	0.0
19	9.488758351688809E-5	0.0	0.0	2.5458022365636017	0.0
20	9.488758351688809E-5	0.0	0.0	2.669693791442152	0.0
21	9.488758351688809E-5	0.0	0.0	2.8176235341449805	0.0
22	9.488758351688809E-5	0.0	0.0	2.984151243217119	0.0
23	9.488758351688809E-5	0.0	0.0	3.155707994215653	0.0
24	9.488758351688809E-5	0.0	0.0	3.2869691514140147	0.0
25	9.488758351688809E-5	0.0	0.0	3.406306102283754	0.0
26	9.488758351688809E-5	0.0	0.0	3.525390019597449	0.0
27	9.488758351688809E-5	0.0	0.0	3.6425129268517944	0.0
28	9.488758351688809E-5	0.0	0.0	3.770041839098492	0.0
29	9.488758351688809E-5	0.0	0.0	3.9123415851793184	0.0
30	9.488758351688809E-5	0.0	0.0	4.073871881519568	0.0
31	9.488758351688809E-5	0.0	0.0	4.232207629214749	0.0
32	9.488758351688809E-5	0.0	0.0	4.372577994430731	0.0
33	9.488758351688809E-5	0.0	0.0	4.513517685147816	0.0
34	9.488758351688809E-5	0.0	0.0	4.667456974806714	0.0
35	1.2651677802251744E-4	0.0	0.0	4.838982096610742	0.0
36	1.2651677802251744E-4	0.0	0.0	5.000575651340003	0.0
37	1.2651677802251744E-4	0.0	0.0	5.163466003043994	0.0
38	1.2651677802251744E-4	0.0	0.0	5.344732916755755	0.0
39	1.2651677802251744E-4	0.0	0.0	5.571261207805073	0.0
40	1.2651677802251744E-4	0.0	0.0	5.771315863053179	0.0
41	1.2651677802251744E-4	0.0	0.0	5.984528263215626	0.0
42	1.2651677802251744E-4	0.0	0.0	6.183254492294496	0.0
43	1.2651677802251744E-4	0.0	0.0	6.361042194610638	0.0
44	1.2651677802251744E-4	0.0	0.0	6.547021858303739	0.0
45	1.2651677802251744E-4	0.0	0.0	6.7324954548847495	0.0
46	1.2651677802251744E-4	0.0	0.0	6.94763723591204	0.0
47	1.2651677802251744E-4	0.0	0.0	7.165056318943737	0.0
48	1.2651677802251744E-4	0.0	0.0	7.382317256002905	0.0
49	1.2651677802251744E-4	0.0	0.0	7.615076498369831	0.0
50	1.5814597252814682E-4	0.0	0.0	7.842648552837835	0.0
51	1.5814597252814682E-4	0.0	0.0	8.07230813414321	0.0
52	1.8977516703377618E-4	0.0	0.0	8.328852530778368	0.0
53	1.8977516703377618E-4	0.0	0.0	8.568506937547523	0.0
54	2.2140436153940556E-4	0.0	0.0	8.818219428169467	0.0
55	2.2140436153940556E-4	0.0	0.0	9.057842205744114	0.0
56	2.2140436153940556E-4	0.0	0.0	9.313848906072678	0.0
57	2.2140436153940556E-4	0.0	0.0	9.557836512489104	0.0
58	2.2140436153940556E-4	0.0	0.0	9.80071709709783	0.0
59	2.2140436153940556E-4	0.0	0.0	10.052105935028575	0.0
60	2.2140436153940556E-4	0.0	0.0	10.311908138697813	0.0
61	2.2140436153940556E-4	0.0	0.0	10.581199100718742	0.0
62	2.2140436153940556E-4	0.0	0.0	10.878608416655174	0.0
63	2.2140436153940556E-4	0.0	0.0	11.190155982535623	0.0
64	2.2140436153940556E-4	0.0	0.0	11.483769795131382	0.0
65	2.530335560450349E-4	0.0	0.0	11.757931653106176	0.0
66	2.530335560450349E-4	0.0	0.0	12.022383348367743	0.0
67	2.530335560450349E-4	0.0	0.0	12.28329257384468	0.0
68	2.846627505506643E-4	0.0	0.0	12.590506940077859	0.0
69	3.1629194505629365E-4	0.0	0.0	12.935233530994713	0.0
70	3.1629194505629365E-4	0.0	0.0	13.223407122135502	0.0
71	3.1629194505629365E-4	0.0	0.0	13.513510094141134	0.0
72	3.1629194505629365E-4	0.0	0.0	13.801873460448958	0.0
73	3.47921139561923E-4	0.0	0.0	14.108803163931585	0.0
74	3.7955033406755236E-4	0.0	0.0	14.430914880776914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9205	0.0	72.17817	1
TAGGGCG	675	0.0	68.74074	5
CGTAAGG	470	0.0	67.23405	2
AGGGCGA	1710	0.0	66.90058	6
GGGCGAT	2585	0.0	66.84719	7
CGTTAGG	475	0.0	66.52631	2
CGGGTCC	355	0.0	66.478874	6
TAGGTCG	145	0.0	66.2069	1
TACGGGT	485	0.0	65.979385	4
GGCGATC	465	0.0	65.37634	8
GCGAACG	75	0.0	64.0	1
TTACGTA	25	1.2358601E-4	64.0	80
TAATGCG	120	0.0	63.333332	1
GCGGGTC	520	0.0	63.07692	5
GTTACGG	395	0.0	62.784813	2
ACGGGAT	1525	0.0	62.688526	5
TGTTACG	205	0.0	62.439026	1
TCGATAG	315	0.0	62.22222	1
TTACGGG	1165	0.0	61.80257	3
CGAAGGG	1620	0.0	61.48148	3
>>END_MODULE