FastQCFastQC Report
Thu 26 May 2016
SRR1547781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547781_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2334375
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155290.665231593038822No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28050.12016064257028111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.10332530120481928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG750.074.6666641
TACGGGA10150.071.724144
CGTAAGG2050.070.2439042
CGTTTTT76350.068.7884751
GCGCGAC1300.067.692319
TGTAGCG3700.065.9459461
TAGGGAC31300.065.431315
TAGCGGG20600.064.466033
TATAGCG2550.064.313721
CGATAGG4000.064.02
CGTAGGG11150.063.4977533
TTACGGG10950.062.4657553
ATTAGCG2050.062.4390261
TAGGGAT41850.062.2222255
TACCGCG459.429641E-962.2222251
GTAGGGA37100.061.8867874
GTACGGG8300.061.6867453
ACGTAGG4350.061.6091962
TAGGGAG27550.061.415615
CTAGGGA17000.061.4117664
Produced by FastQC (version 0.11.5)