##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547781_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2334375
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865871593038822	31.0	31.0	33.0	31.0	34.0
2	32.314653815261046	33.0	31.0	34.0	31.0	34.0
3	32.4499846854083	34.0	31.0	34.0	31.0	34.0
4	36.04468562248996	37.0	35.0	37.0	35.0	37.0
5	36.007723694779116	37.0	35.0	37.0	35.0	37.0
6	36.06459502008032	37.0	35.0	37.0	35.0	37.0
7	36.29321852744311	37.0	36.0	37.0	35.0	37.0
8	36.24961970548862	37.0	37.0	37.0	35.0	37.0
9	38.119683855421684	39.0	39.0	39.0	37.0	39.0
10-11	37.622012101740296	39.0	37.5	39.0	35.0	39.0
12-13	36.798380294511375	39.0	35.0	39.0	33.0	39.0
14-15	37.82168139223561	40.0	35.0	41.0	33.0	41.0
16-17	38.01550200803213	40.0	35.0	41.0	34.0	41.0
18-19	37.90720985274431	40.0	36.0	41.0	34.0	41.0
20-21	37.73390564926372	40.0	35.0	41.0	33.5	41.0
22-23	37.55122463186078	39.0	35.0	41.0	33.0	41.0
24-25	37.48845815261045	39.0	35.0	41.0	33.0	41.0
26-27	37.47271196787149	39.0	35.0	41.0	33.0	41.0
28-29	37.48519646586345	39.0	35.0	41.0	33.0	41.0
30-31	37.32219095046854	39.0	35.0	41.0	33.0	41.0
32-33	37.02547662650602	39.0	35.0	41.0	32.5	41.0
34-35	36.7358944042838	39.0	35.0	41.0	31.5	41.0
36-37	36.56321349397591	39.0	35.0	41.0	31.0	41.0
38-39	36.47156862115127	39.0	35.0	41.0	31.0	41.0
40-41	36.30080878179384	38.5	35.0	41.0	31.0	41.0
42-43	36.26482248995984	38.5	35.0	41.0	31.0	41.0
44-45	36.17185970548862	38.0	35.0	41.0	30.5	41.0
46-47	36.07602955823293	38.0	35.0	41.0	30.0	41.0
48-49	35.98759796519411	38.0	35.0	40.0	30.0	41.0
50-51	35.81817103078983	38.0	35.0	40.0	30.0	41.0
52-53	35.62131234270415	37.0	35.0	40.0	29.5	41.0
54-55	35.42512621151272	37.0	35.0	40.0	29.0	41.0
56-57	35.14004305220884	36.5	34.5	40.0	28.5	41.0
58-59	34.840909236947795	36.0	34.0	40.0	28.0	41.0
60-61	34.53425949129853	35.5	34.0	39.0	27.0	41.0
62-63	34.138627469879516	35.0	33.5	39.0	26.0	40.5
64-65	33.851012797858104	35.0	33.5	38.5	26.0	40.0
66-67	33.59018859437751	35.0	33.0	38.0	26.0	40.0
68-69	33.15755480589023	35.0	33.0	37.0	24.5	40.0
70-71	32.903627309236946	35.0	33.0	37.0	24.0	39.0
72-73	32.6766843373494	35.0	33.0	36.0	24.0	39.0
74-75	32.38752856760375	35.0	33.0	36.0	23.5	38.5
76-77	30.342515876840697	33.0	29.5	34.5	19.5	36.0
78-79	31.423960374832664	34.0	31.5	35.0	20.0	37.0
80-81	31.674605408299865	35.0	32.0	35.0	20.0	36.5
82-83	31.52636722891566	35.0	32.0	35.0	20.0	36.0
84-85	31.338342811244978	35.0	32.0	35.0	19.5	36.0
86	30.93524390896921	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	18.0
11	12.0
12	46.0
13	88.0
14	288.0
15	807.0
16	2067.0
17	4546.0
18	9524.0
19	14912.0
20	18101.0
21	18616.0
22	16979.0
23	15592.0
24	15489.0
25	17029.0
26	20168.0
27	25175.0
28	31289.0
29	39543.0
30	49995.0
31	63130.0
32	80008.0
33	109466.0
34	276064.0
35	242785.0
36	203031.0
37	350842.0
38	472708.0
39	235214.0
40	835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.69804551539492	24.263668005354752	29.237633199464526	13.80065327978581
2	30.605708165997324	25.860112449799193	30.939973226238287	12.594206157965193
3	28.711282463186077	25.221054886211512	33.32305220883534	12.744610441767069
4	26.60613654618474	27.276380187416333	32.207250334672025	13.910232931726906
5	24.040653279785808	31.295357429718873	31.192160642570283	13.471828647925033
6	22.774661311914322	39.94593842034806	26.641906291834	10.637493975903615
7	88.26431057563588	3.5093761713520752	6.585831325301204	1.6404819277108436
8	88.83362784471218	3.1543775100401605	6.276883534136546	1.7351111111111113
9	84.51311378848729	5.424835341365462	7.580230254350736	2.4818206157965195
10-11	41.19121820615796	28.16340562248996	19.43256760374833	11.212808567603748
12-13	30.163791164658633	25.52869611780455	27.918543507362788	16.38896921017403
14-15	16.96314859437751	31.93872021419009	34.733258366800534	16.36487282463186
16-17	22.40027844712182	26.081092369477908	32.237686746987954	19.280942436412314
18-19	24.398372155287817	27.86533868808568	30.20671485943775	17.529574297188756
20-21	26.526757697456492	28.570923694779115	28.872803212851405	16.029515394912984
22-23	21.529274431057562	24.69410441767068	32.67283534136546	21.10378580990629
24-25	20.222607764390897	27.635855421686745	34.12170281124498	18.019834002677378
26-27	17.8334672021419	33.5337295850067	30.239357429718876	18.39344578313253
28-29	17.001103078982595	28.88182061579652	35.54891566265061	18.56816064257028
30-31	22.65368674698795	28.581847389558234	30.478350736278447	18.286115127175368
32-33	26.125601070950466	28.309997322623833	28.602580990629185	16.96182061579652
34-35	20.136203480589025	28.684530120481927	31.12319143239625	20.056074966532798
36-37	22.845408299866133	30.34467469879518	29.870029451137885	16.9398875502008
38-39	20.3514859437751	31.789472556894243	27.862704149933066	19.996337349397592
40-41	20.944171352074967	27.373108433734938	29.03687282463186	22.64584738955823
42-43	20.566468540829987	27.532615796519412	28.758211512717537	23.142704149933067
44-45	19.85441499330656	31.323223560910307	28.40130655957162	20.42105488621151
46-47	21.57772423025435	30.259448460508704	28.154302543507363	20.008524765729586
48-49	21.020444444444443	28.114720214190093	31.04659705488621	19.81823828647925
50-51	19.651726907630522	31.1735046854083	29.112589022757696	20.06217938420348
52-53	22.26960107095047	27.261729585006695	30.150875502008034	20.317793842034806
54-55	22.49280856760375	27.28109236947791	30.852283801874165	19.37381526104418
56-57	22.195555555555554	26.486318607764392	29.140690763052206	22.177435073627844
58-59	24.03645515394913	28.557665327978583	27.868080321285138	19.537799196787148
60-61	24.621493975903615	29.14338955823293	26.9560374832664	19.279078982597056
62-63	22.965354752342705	26.7800374832664	30.585659973226235	19.66894779116466
64-65	20.44515127175368	30.2589344042838	30.549161981258365	18.746752342704152
66-67	20.051791164658635	29.366961178045514	26.545670682730925	24.035576974564925
68-69	18.826409638554217	32.01816331994645	26.411330655957162	22.744096385542168
70-71	19.316562248995982	30.671078982597056	29.326457831325303	20.685900937081662
72-73	22.403062918340026	30.744953145917002	27.42498527443106	19.426998661311913
74-75	21.004123159303884	32.22956894243641	25.285868808567603	21.4804390896921
76-77	25.373386880856764	28.46504953145917	26.638500669344044	19.523062918340024
78-79	19.690024096385542	29.74318607764391	29.559282463186076	21.007507362784473
80-81	17.935228915662652	29.13942704149933	30.004605087014724	22.920738955823296
82-83	19.420251673360106	28.50799464524766	30.782586345381524	21.28916733601071
84-85	18.091116465863454	27.655175368139222	29.733954484605086	24.51975368139224
86	19.333954484605087	27.738216867469877	30.53399732262383	22.393831325301207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1957.0
1	2371.5
2	6526.5
3	6679.5
4	3520.0
5	4471.0
6	5499.5
7	6005.0
8	6333.0
9	6571.5
10	6233.5
11	5870.5
12	5705.0
13	5691.5
14	5729.0
15	5735.5
16	5680.0
17	5671.5
18	5997.5
19	7384.5
20	8021.0
21	7543.0
22	7619.5
23	9061.5
24	12254.5
25	14141.5
26	14888.0
27	17429.0
28	25076.0
29	30869.0
30	32923.0
31	38744.0
32	44793.0
33	50717.0
34	53631.5
35	58850.0
36	64796.0
37	64171.0
38	67581.5
39	74762.0
40	85151.0
41	98547.0
42	108962.0
43	113225.0
44	118005.5
45	123214.5
46	130833.0
47	133719.5
48	129143.5
49	118114.5
50	107358.0
51	94961.5
52	78200.0
53	70185.5
54	64672.0
55	60961.0
56	55212.5
57	51311.0
58	45331.5
59	33787.5
60	25425.5
61	21104.0
62	16569.5
63	11203.0
64	8847.0
65	6693.5
66	3854.5
67	2722.5
68	2089.5
69	1562.0
70	955.5
71	690.0
72	583.5
73	394.0
74	249.0
75	122.5
76	73.5
77	71.0
78	37.0
79	21.0
80	20.0
81	15.5
82	10.0
83	10.5
84	8.0
85	3.5
86	4.0
87	3.0
88	4.0
89	3.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2334375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.543597645388713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41254726929216	12.343566827492893
2	8.662094623206043	2.6928022717879934
3	2.6615206575309585	1.2410881867665486
4	1.2392127388702872	0.7704729684015957
5	0.8092938253860258	0.6289668799348928
6	0.5411690594399738	0.5047028470841041
7	0.4205430820094089	0.45757267215141717
8	0.3351452939884766	0.41674908820019146
9	0.2658901257854518	0.3719600219781771
>10	2.6112108154634313	9.309567444987188
>50	1.1103261280168142	12.613618638570731
>100	1.9168720336549046	56.20328984320684
>500	0.011117135181251498	1.0828529309750468
>1k	0.0027792837953128746	0.6919355060810389
>5k	0.0	0.0
>10k+	2.7792837953128746E-4	0.6708538723813731
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15529	0.665231593038822	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2805	0.12016064257028111	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2412	0.10332530120481928	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.28380187416332E-5	0.0	0.0	0.04755020080321285	0.0
2	4.28380187416332E-5	0.0	0.0	0.131855421686747	0.0
3	4.28380187416332E-5	0.0	0.0	0.1922570281124498	0.0
4	4.28380187416332E-5	0.0	0.0	0.3028219544846051	0.0
5	4.28380187416332E-5	0.0	0.0	0.5438714859437751	0.0
6	4.28380187416332E-5	0.0	0.0	0.7276037483266399	0.0
7	4.28380187416332E-5	0.0	0.0	0.8532476572958501	0.0
8	4.28380187416332E-5	0.0	0.0	1.111475234270415	0.0
9	4.28380187416332E-5	0.0	0.0	1.2010923694779116	0.0
10	4.28380187416332E-5	0.0	0.0	1.4161820615796519	0.0
11	4.28380187416332E-5	0.0	0.0	1.7143775100401606	0.0
12	4.28380187416332E-5	0.0	0.0	1.953156626506024	0.0
13	4.28380187416332E-5	0.0	0.0	2.029408299866131	0.0
14	4.28380187416332E-5	0.0	0.0	2.0688621151271755	0.0
15	4.28380187416332E-5	0.0	0.0	2.125065595716198	0.0
16	4.28380187416332E-5	0.0	0.0	2.228562248995984	0.0
17	4.28380187416332E-5	0.0	0.0	2.3638447121820616	0.0
18	4.28380187416332E-5	0.0	0.0	2.523202141900937	0.0
19	4.28380187416332E-5	0.0	0.0	2.6403212851405624	0.0
20	4.28380187416332E-5	0.0	0.0	2.760995983935743	0.0
21	4.28380187416332E-5	0.0	0.0	2.8982918340026775	0.0
22	4.28380187416332E-5	0.0	0.0	3.0563212851405623	0.0
23	4.28380187416332E-5	0.0	0.0	3.213965194109772	0.0
24	4.28380187416332E-5	0.0	0.0	3.3325836680053547	0.0
25	4.28380187416332E-5	0.0	0.0	3.441263721552878	0.0
26	4.28380187416332E-5	0.0	0.0	3.548144578313253	0.0
27	4.28380187416332E-5	0.0	0.0	3.6871967871485944	0.0
28	4.28380187416332E-5	0.0	0.0	3.8112128514056223	0.0
29	4.28380187416332E-5	0.0	0.0	3.965215528781794	0.0
30	4.28380187416332E-5	0.0	0.0	4.11489156626506	0.0
31	4.28380187416332E-5	0.0	0.0	4.253858099062918	0.0
32	4.28380187416332E-5	0.0	0.0	4.38584203480589	0.0
33	4.28380187416332E-5	0.0	0.0	4.518382864792503	0.0
34	4.28380187416332E-5	0.0	0.0	4.671228915662651	0.0
35	4.28380187416332E-5	0.0	0.0	4.838082998661312	0.0
36	4.28380187416332E-5	0.0	0.0	4.987930388219545	0.0
37	4.28380187416332E-5	0.0	0.0	5.152085676037483	0.0
38	4.28380187416332E-5	0.0	0.0	5.325751004016064	0.0
39	4.28380187416332E-5	0.0	0.0	5.611480589022758	0.0
40	4.28380187416332E-5	0.0	0.0	5.794955823293173	0.0
41	8.56760374832664E-5	0.0	0.0	5.99004016064257	0.0
42	8.56760374832664E-5	0.0	0.0	6.190393574297189	0.0
43	8.56760374832664E-5	0.0	0.0	6.36170281124498	0.0
44	8.56760374832664E-5	0.0	0.0	6.524273092369478	0.0
45	8.56760374832664E-5	0.0	0.0	6.690270414993306	0.0
46	8.56760374832664E-5	0.0	0.0	6.878800535475234	0.0
47	8.56760374832664E-5	0.0	0.0	7.077697456492637	0.0
48	8.56760374832664E-5	0.0	0.0	7.262672021419009	0.0
49	8.56760374832664E-5	0.0	0.0	7.4580133868808565	0.0
50	1.285140562248996E-4	0.0	0.0	7.662821954484605	0.0
51	1.285140562248996E-4	0.0	0.0	7.87041499330656	0.0
52	1.285140562248996E-4	0.0	0.0	8.11566265060241	0.0
53	1.285140562248996E-4	0.0	0.0	8.330966532797857	0.0
54	1.285140562248996E-4	0.0	0.0	8.540915662650603	0.0
55	1.285140562248996E-4	0.0	0.0	8.760032128514057	0.0
56	1.285140562248996E-4	0.0	0.0	8.98334672021419	0.0
57	1.285140562248996E-4	0.0	0.0	9.210688085676038	0.0
58	1.285140562248996E-4	0.0	0.0	9.425734939759035	0.0
59	1.285140562248996E-4	0.0	0.0	9.649435073627846	0.0
60	1.285140562248996E-4	0.0	0.0	9.899180722891566	0.0
61	1.713520749665328E-4	0.0	0.0	10.164862115127175	0.0
62	1.713520749665328E-4	0.0	0.0	10.488289156626506	0.0
63	1.713520749665328E-4	0.0	0.0	10.770420348058902	0.0
64	1.713520749665328E-4	0.0	0.0	11.041713520749665	0.0
65	1.713520749665328E-4	0.0	0.0	11.2860187416332	0.0
66	2.14190093708166E-4	0.0	0.0	11.516487282463187	0.0
67	2.14190093708166E-4	0.0	0.0	11.761991967871486	0.0
68	2.14190093708166E-4	0.0	0.0	12.069868808567604	0.0
69	2.14190093708166E-4	0.0	0.0	12.493322623828648	0.0
70	2.14190093708166E-4	0.0	0.0	12.77742436412316	0.0
71	2.570281124497992E-4	0.0	0.0	13.045633199464525	0.0
72	2.570281124497992E-4	0.0	0.0	13.31071485943775	0.0
73	2.9986613119143237E-4	0.0	0.0	13.594987951807228	0.0
74	2.9986613119143237E-4	0.0	0.0	13.907319946452477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCG	75	0.0	74.666664	1
TACGGGA	1015	0.0	71.72414	4
CGTAAGG	205	0.0	70.243904	2
CGTTTTT	7635	0.0	68.788475	1
GCGCGAC	130	0.0	67.69231	9
TGTAGCG	370	0.0	65.945946	1
TAGGGAC	3130	0.0	65.43131	5
TAGCGGG	2060	0.0	64.46603	3
TATAGCG	255	0.0	64.31372	1
CGATAGG	400	0.0	64.0	2
CGTAGGG	1115	0.0	63.497753	3
TTACGGG	1095	0.0	62.465755	3
ATTAGCG	205	0.0	62.439026	1
TAGGGAT	4185	0.0	62.222225	5
TACCGCG	45	9.429641E-9	62.222225	1
GTAGGGA	3710	0.0	61.886787	4
GTACGGG	830	0.0	61.686745	3
ACGTAGG	435	0.0	61.609196	2
TAGGGAG	2755	0.0	61.41561	5
CTAGGGA	1700	0.0	61.411766	4
>>END_MODULE